Ureibacillus thermophilus
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2797 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P6UUM2|A0A4P6UUM2_9BACL Uncharacterized protein OS=Ureibacillus thermophilus OX=367743 GN=DKZ56_14145 PE=4 SV=1
MM1 pKa = 7.45 ALEE4 pKa = 4.01 ILKK7 pKa = 10.38 LAINASTTVATVPDD21 pKa = 3.85 VKK23 pKa = 10.93 KK24 pKa = 10.64 FFYY27 pKa = 10.59 LVNSTVTGEE36 pKa = 4.15 DD37 pKa = 3.21 TLTIYY42 pKa = 10.99 AKK44 pKa = 10.9 DD45 pKa = 3.75 FMDD48 pKa = 3.87 DD49 pKa = 3.21 TGTVGVTLPEE59 pKa = 4.11 LSANNSYY66 pKa = 8.97 LTVYY70 pKa = 10.57 VNGVQVMQDD79 pKa = 3.38 LLTYY83 pKa = 10.9 NPGEE87 pKa = 4.52 DD88 pKa = 3.73 EE89 pKa = 4.73 NGLLTIAVPEE99 pKa = 4.01 EE100 pKa = 4.11 SEE102 pKa = 4.43 IIQNSPVVLVVTNFAPTAQTTIEE125 pKa = 4.16 TT126 pKa = 4.06
Molecular weight: 13.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.783
IPC_protein 3.681
Toseland 3.49
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.592
Rodwell 3.516
Grimsley 3.414
Solomon 3.63
Lehninger 3.592
Nozaki 3.795
DTASelect 3.935
Thurlkill 3.554
EMBOSS 3.605
Sillero 3.795
Patrickios 1.837
IPC_peptide 3.63
IPC2_peptide 3.77
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A4P6URV4|A0A4P6URV4_9BACL AMP-binding protein OS=Ureibacillus thermophilus OX=367743 GN=DKZ56_09025 PE=4 SV=1
MM1 pKa = 8.0 RR2 pKa = 11.84 ILPPFVRR9 pKa = 11.84 LLLQLVRR16 pKa = 11.84 ILPPFVRR23 pKa = 11.84 LLPQLVRR30 pKa = 11.84 ILPPFVRR37 pKa = 11.84 LLPHH41 pKa = 6.98 LVRR44 pKa = 11.84 ILPPFARR51 pKa = 11.84 LLLHH55 pKa = 6.81 LVQILSKK62 pKa = 9.7 IKK64 pKa = 10.01 KK65 pKa = 9.97 VSFQKK70 pKa = 10.97 YY71 pKa = 6.38 FF72 pKa = 3.11
Molecular weight: 8.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.994
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.208
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.945
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.057
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2797
0
2797
810556
29
1492
289.8
32.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.991 ± 0.05
0.771 ± 0.014
4.811 ± 0.034
8.229 ± 0.057
4.54 ± 0.042
6.488 ± 0.047
2.076 ± 0.019
8.273 ± 0.048
7.427 ± 0.043
9.536 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.688 ± 0.021
4.447 ± 0.036
3.65 ± 0.031
3.689 ± 0.032
4.157 ± 0.034
5.497 ± 0.03
5.166 ± 0.033
6.988 ± 0.033
0.931 ± 0.015
3.646 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here