Streptomyces phage Faust

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Annadreamyvirus; unclassified Annadreamyvirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9UZ89|A0A7G9UZ89_9CAUD Uncharacterized protein OS=Streptomyces phage Faust OX=2767565 GN=272 PE=4 SV=1
MM1 pKa = 8.07PYY3 pKa = 10.62GIAQEE8 pKa = 4.93DD9 pKa = 3.66TAEE12 pKa = 4.37WIKK15 pKa = 11.26SRR17 pKa = 11.84ANDD20 pKa = 3.54GNVSDD25 pKa = 5.1RR26 pKa = 11.84IEE28 pKa = 4.29LEE30 pKa = 4.28SEE32 pKa = 3.71KK33 pKa = 11.12SNYY36 pKa = 7.73TLSVIIDD43 pKa = 3.43GALYY47 pKa = 10.74EE48 pKa = 5.42EE49 pKa = 4.64FDD51 pKa = 3.66FWSNGYY57 pKa = 9.52AVNVADD63 pKa = 4.24WVAEE67 pKa = 3.85LTEE70 pKa = 4.5RR71 pKa = 11.84DD72 pKa = 4.11GITDD76 pKa = 3.6YY77 pKa = 11.74DD78 pKa = 4.29VMVSEE83 pKa = 5.34IDD85 pKa = 4.7SPDD88 pKa = 3.3CLYY91 pKa = 10.71RR92 pKa = 11.84KK93 pKa = 10.11SGFTYY98 pKa = 10.69

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9UYZ6|A0A7G9UYZ6_9CAUD Uncharacterized protein OS=Streptomyces phage Faust OX=2767565 GN=175 PE=4 SV=1
MM1 pKa = 7.19SRR3 pKa = 11.84LVRR6 pKa = 11.84FARR9 pKa = 11.84AVAEE13 pKa = 4.36KK14 pKa = 8.64PTEE17 pKa = 4.47AIEE20 pKa = 4.96GIIAMAVTLVGIWFISPFYY39 pKa = 10.56KK40 pKa = 10.18ATTSVSAQAWANGTIPQYY58 pKa = 10.62IGAFQAVVGAILLFALVKK76 pKa = 10.56KK77 pKa = 10.37SWARR81 pKa = 11.84RR82 pKa = 11.84QAARR86 pKa = 11.84RR87 pKa = 11.84LTTFAIFILYY97 pKa = 9.85LFYY100 pKa = 11.12GLSSTIILGLGRR112 pKa = 11.84VSWIATFALALIAGVAHH129 pKa = 7.28LRR131 pKa = 11.84LKK133 pKa = 10.13WEE135 pKa = 4.5EE136 pKa = 3.97GNHH139 pKa = 6.16ARR141 pKa = 11.84DD142 pKa = 3.44

Molecular weight: 15.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

238

0

238

36866

19

2101

154.9

17.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.148 ± 0.348

1.012 ± 0.115

6.483 ± 0.164

7.112 ± 0.312

4.053 ± 0.15

7.59 ± 0.248

1.896 ± 0.141

5.455 ± 0.129

6.103 ± 0.237

6.925 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.112

4.497 ± 0.138

3.711 ± 0.155

3.233 ± 0.167

5.607 ± 0.192

5.729 ± 0.171

6.223 ± 0.349

7.367 ± 0.172

1.975 ± 0.105

4.058 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski