Flavobacterium rivuli WB 3.3-2 = DSM 21788

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium; Flavobacterium rivuli

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3691 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2M1V0|A0A0A2M1V0_9FLAO Uncharacterized protein OS=Flavobacterium rivuli WB 3.3-2 = DSM 21788 OX=1121895 GN=Q765_11595 PE=4 SV=1
MM1 pKa = 7.39VLFAVLGGIVASCEE15 pKa = 4.2TEE17 pKa = 3.93PVDD20 pKa = 5.41PLLLTDD26 pKa = 5.25DD27 pKa = 4.3NGGTDD32 pKa = 4.16TIADD36 pKa = 3.75PGIFTVKK43 pKa = 10.1IGGTLFVADD52 pKa = 3.94STIATLGNGQIVIKK66 pKa = 10.2GYY68 pKa = 10.64KK69 pKa = 7.99GTEE72 pKa = 4.18GEE74 pKa = 4.31NVTITVQATTTGTYY88 pKa = 7.15TALMVYY94 pKa = 10.47DD95 pKa = 5.5PGTSDD100 pKa = 3.45NFYY103 pKa = 11.16SNISPASGPSGSATITTINTAARR126 pKa = 11.84VISGSFSFTGYY137 pKa = 11.0YY138 pKa = 10.19SDD140 pKa = 4.02TAQNLPNVAFTEE152 pKa = 5.11GIFTNISYY160 pKa = 11.03GGGTPPTNPSGPGVFTASVDD180 pKa = 3.78GAPFTAAVTNAVLGSGLFSIQGVDD204 pKa = 3.25ATGKK208 pKa = 9.91AISIAVEE215 pKa = 3.9EE216 pKa = 4.63TTPGTYY222 pKa = 9.35TGSQVLLAYY231 pKa = 10.12YY232 pKa = 10.3EE233 pKa = 4.22NATSEE238 pKa = 4.18DD239 pKa = 4.29SYY241 pKa = 12.1SNLLNDD247 pKa = 4.06TGTLTITSIDD257 pKa = 4.01TVNHH261 pKa = 6.9TITGTFSFTGDD272 pKa = 3.41YY273 pKa = 11.17SDD275 pKa = 4.12IDD277 pKa = 3.55NPRR280 pKa = 11.84TAKK283 pKa = 10.64VITSGVFTNVPYY295 pKa = 10.76VGSIDD300 pKa = 3.48NTDD303 pKa = 2.91IFTATVNGTAITYY316 pKa = 9.47GGSDD320 pKa = 3.7LVIGFIDD327 pKa = 3.63VNEE330 pKa = 4.65FSAVTLRR337 pKa = 11.84GTNATHH343 pKa = 7.25VIEE346 pKa = 4.23LQINEE351 pKa = 4.25DD352 pKa = 4.32TPVGTYY358 pKa = 10.32PFTDD362 pKa = 3.29ATNALPKK369 pKa = 10.0ATFTDD374 pKa = 4.25TSAPDD379 pKa = 4.73DD380 pKa = 3.21ITYY383 pKa = 10.76NITRR387 pKa = 11.84GSLVITANDD396 pKa = 3.69GVRR399 pKa = 11.84IKK401 pKa = 10.37GTFSFEE407 pKa = 3.76VRR409 pKa = 11.84NPANPNGAPLHH420 pKa = 5.63TVTGGTFDD428 pKa = 4.1IEE430 pKa = 4.32FF431 pKa = 4.31

Molecular weight: 44.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2M9B3|A0A0A2M9B3_9FLAO Uncharacterized protein OS=Flavobacterium rivuli WB 3.3-2 = DSM 21788 OX=1121895 GN=Q765_03940 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.39KK3 pKa = 10.22IIFTVAAIFAMGIASAQTNPTRR25 pKa = 11.84VPGTTTPTTTSPTTTSPTTTNGTINNGTNPNGTLNNGTTNGTLNNGVNNGTLNNPNTVPVPSTTTRR91 pKa = 11.84QNNNTRR97 pKa = 11.84STAIPGTTSPSTTSPSTTNNNAIRR121 pKa = 11.84PQTTASPSPTSTSTAVPSTSTRR143 pKa = 11.84MPASGTTTTTTTPSSTGTTPQPP165 pKa = 3.39

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3691

0

3691

1210709

41

3502

328.0

36.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.648 ± 0.043

0.753 ± 0.013

5.498 ± 0.03

5.912 ± 0.045

4.961 ± 0.032

6.743 ± 0.038

1.771 ± 0.019

7.401 ± 0.037

7.201 ± 0.05

9.011 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.018

5.952 ± 0.036

3.653 ± 0.025

3.441 ± 0.021

3.443 ± 0.025

5.982 ± 0.036

6.469 ± 0.064

6.568 ± 0.032

1.06 ± 0.017

4.299 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski