bacteria symbiont BFo1 of Frankliniella occidentalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; unclassified Erwiniaceae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J8YC16|A0A0J8YC16_9GAMM Thioredoxin-like_fold domain-containing protein OS=bacteria symbiont BFo1 of Frankliniella occidentalis OX=1628855 GN=AI28_17150 PE=4 SV=1
VV1 pKa = 6.7PAADD5 pKa = 3.56VAKK8 pKa = 10.2WPAGQLAIVVSGADD22 pKa = 3.13ASGNPVTITQNITVDD37 pKa = 4.08LSSVAISVDD46 pKa = 3.99TIASDD51 pKa = 4.55GIINAAEE58 pKa = 4.07KK59 pKa = 10.63AADD62 pKa = 3.84LTLSGTTQNVEE73 pKa = 3.63AGRR76 pKa = 11.84DD77 pKa = 3.56VSVNFAGHH85 pKa = 6.18QYY87 pKa = 5.56TTRR90 pKa = 11.84VQADD94 pKa = 4.15GSWSITVAAADD105 pKa = 3.76MKK107 pKa = 11.04ALKK110 pKa = 10.79DD111 pKa = 3.35GDD113 pKa = 3.88ATVSVSVSNAAGNGATAANTAQVDD137 pKa = 4.04TAAPTLTINTIAGNNVLNGAEE158 pKa = 4.07ANADD162 pKa = 3.58VAIGGTSTAEE172 pKa = 3.86AGQQVTLTLNGQTYY186 pKa = 6.97TASVSADD193 pKa = 3.62GTWSTTLPAASAGALADD210 pKa = 3.45GTYY213 pKa = 9.42TVSASVSDD221 pKa = 3.34IAGNSSRR228 pKa = 11.84ATGSLLVDD236 pKa = 3.33VTAPVVTINTVAGDD250 pKa = 3.92DD251 pKa = 3.76VLNTAEE257 pKa = 4.29HH258 pKa = 6.2AQAQVITGGSTGTQAGNLVTLVIDD282 pKa = 3.56GHH284 pKa = 6.15TYY286 pKa = 7.03TTVVDD291 pKa = 4.72ARR293 pKa = 11.84GNWSIGVPASAISALASGDD312 pKa = 3.41TTFTVSITDD321 pKa = 3.45NAGNTGSTDD330 pKa = 3.22HH331 pKa = 7.4LITVMTDD338 pKa = 3.02ALSLSIDD345 pKa = 4.2TIAQDD350 pKa = 4.88DD351 pKa = 4.56IINAAEE357 pKa = 4.39KK358 pKa = 10.88GNSLTLSGSSEE369 pKa = 3.92KK370 pKa = 10.59LVSGTEE376 pKa = 3.99VAVVLNGKK384 pKa = 7.66TYY386 pKa = 9.54TVQVDD391 pKa = 3.64VKK393 pKa = 11.03GDD395 pKa = 3.38WTLSIPATDD404 pKa = 3.5VAKK407 pKa = 9.64LTDD410 pKa = 3.72GYY412 pKa = 9.82YY413 pKa = 9.99TVTASASDD421 pKa = 3.51TAGNPVSATHH431 pKa = 7.0DD432 pKa = 3.62VAVDD436 pKa = 3.68TTAPALLINPIAADD450 pKa = 4.8GILNAAEE457 pKa = 4.48LGQALTLSGTTTAQAGQTVHH477 pKa = 5.15VTLNSVTYY485 pKa = 6.62TTQVSADD492 pKa = 4.0GLWSVQVPQNDD503 pKa = 3.26VAALQDD509 pKa = 3.5GTLTVTASTSDD520 pKa = 3.49VAGNPASANASVIVDD535 pKa = 3.79TLAPTLTINTIAGDD549 pKa = 4.19DD550 pKa = 4.07IINSAEE556 pKa = 3.8QHH558 pKa = 5.92AGQAIGGTTSAEE570 pKa = 3.89AGQTVTVTFNHH581 pKa = 6.51RR582 pKa = 11.84EE583 pKa = 3.83YY584 pKa = 10.37HH585 pKa = 5.41ATVASDD591 pKa = 3.77GTWSVTVPAADD602 pKa = 3.71FLGAADD608 pKa = 4.19GSYY611 pKa = 9.35TVSASVSDD619 pKa = 3.85VAGNPVSAASSVTLSGEE636 pKa = 3.91MPTISINTFAQDD648 pKa = 3.58DD649 pKa = 4.26VVNAAEE655 pKa = 5.02HH656 pKa = 5.59GTSLAISGTTTAPANSLVTVTLNGQTYY683 pKa = 5.98TTSVAADD690 pKa = 3.94LSWTLNLSANAVSALADD707 pKa = 3.69GNNYY711 pKa = 10.34AITASVTNSIGNTGSDD727 pKa = 2.71IHH729 pKa = 6.81EE730 pKa = 4.32IAVDD734 pKa = 3.8TTAPSMTITIDD745 pKa = 3.53SLSEE749 pKa = 3.63DD750 pKa = 3.56TGRR753 pKa = 11.84SNTDD757 pKa = 3.39FVTSDD762 pKa = 3.01NTITLKK768 pKa = 11.01GGLSAALGNAEE779 pKa = 4.23SAQISLDD786 pKa = 4.17GGISWQTLTVVGTTWSYY803 pKa = 12.08APGSALADD811 pKa = 3.3GSYY814 pKa = 9.73TYY816 pKa = 10.97NVRR819 pKa = 11.84VIDD822 pKa = 3.57AAGNVGSTATKK833 pKa = 9.29TVVIDD838 pKa = 3.94TVAPTASQSITIDD851 pKa = 3.16AVSQDD856 pKa = 3.74TGLSSTDD863 pKa = 4.83FITSDD868 pKa = 2.92NTINLRR874 pKa = 11.84GTLGAALNADD884 pKa = 4.47EE885 pKa = 4.65YY886 pKa = 11.47AQISIDD892 pKa = 5.73GGTTWKK898 pKa = 10.57DD899 pKa = 3.41VNISGLTWTYY909 pKa = 10.47IDD911 pKa = 4.7SRR913 pKa = 11.84TLADD917 pKa = 3.49GDD919 pKa = 4.01YY920 pKa = 8.9NYY922 pKa = 10.92QMRR925 pKa = 11.84VVDD928 pKa = 3.85LAGNVGATASQVVTIDD944 pKa = 3.2TVAPDD949 pKa = 3.34SSKK952 pKa = 10.02TISIDD957 pKa = 3.23AVSNDD962 pKa = 3.63TGLSSSDD969 pKa = 3.33FVTSDD974 pKa = 2.77TSLTVSGTLGATLAADD990 pKa = 4.7EE991 pKa = 4.37YY992 pKa = 11.57AQISLDD998 pKa = 5.09GGVTWLNTTTIGTRR1012 pKa = 11.84WRR1014 pKa = 11.84YY1015 pKa = 9.84VDD1017 pKa = 5.78DD1018 pKa = 3.69RR1019 pKa = 11.84TLTDD1023 pKa = 3.4GDD1025 pKa = 3.48YY1026 pKa = 9.09TYY1028 pKa = 11.44YY1029 pKa = 10.93VRR1031 pKa = 11.84VVDD1034 pKa = 3.6AAGNVGSISSQVVTVDD1050 pKa = 3.26TVAPDD1055 pKa = 3.15SSKK1058 pKa = 9.97TVTIDD1063 pKa = 4.73SIATDD1068 pKa = 3.74TGVSASDD1075 pKa = 4.92FITSDD1080 pKa = 2.82TSLTVTGSIGQALGAGEE1097 pKa = 4.36VVQISIDD1104 pKa = 3.64NGASWTTVALSGLKK1118 pKa = 9.24WSYY1121 pKa = 11.5SDD1123 pKa = 5.18GRR1125 pKa = 11.84TLTDD1129 pKa = 3.21GQTLYY1134 pKa = 10.52QVRR1137 pKa = 11.84VVDD1140 pKa = 3.48QAGNVGQTAQQVVTIDD1156 pKa = 3.3TTAPSQAITIGAIGTDD1172 pKa = 3.34TGDD1175 pKa = 3.47NSHH1178 pKa = 7.28DD1179 pKa = 4.41FITSDD1184 pKa = 3.17TTLTFSGNLSATLASDD1200 pKa = 3.54EE1201 pKa = 4.15WAQISLDD1208 pKa = 4.45GGQTWIDD1215 pKa = 3.32VVTRR1219 pKa = 11.84GRR1221 pKa = 11.84TWSYY1225 pKa = 11.27DD1226 pKa = 3.29ATGTALGDD1234 pKa = 3.27GVYY1237 pKa = 10.18NVQVRR1242 pKa = 11.84VIDD1245 pKa = 3.8LAGNTGAVGSQLVTVDD1261 pKa = 3.52TLNPEE1266 pKa = 4.11AQATIASYY1274 pKa = 11.42SDD1276 pKa = 3.18NKK1278 pKa = 10.66GDD1280 pKa = 3.69RR1281 pKa = 11.84QGSFGSGTHH1290 pKa = 5.76TDD1292 pKa = 3.18EE1293 pKa = 4.54TAPTLSGTLSKK1304 pKa = 10.98ALADD1308 pKa = 3.92GEE1310 pKa = 4.42VLKK1313 pKa = 10.43IYY1315 pKa = 10.7RR1316 pKa = 11.84NGVYY1320 pKa = 10.27VGVATVVGLAWTFSDD1335 pKa = 4.49SALADD1340 pKa = 3.15GTYY1343 pKa = 9.83IYY1345 pKa = 10.99VATSQDD1351 pKa = 3.08LAGNVTSSSDD1361 pKa = 3.32FTLTVDD1367 pKa = 3.5TSIPTTIATVTGEE1380 pKa = 4.34STQDD1384 pKa = 3.05TTPIISGTLSEE1395 pKa = 4.43ALGEE1399 pKa = 4.18GMYY1402 pKa = 10.52VQVVVNGVTYY1412 pKa = 9.91TSEE1415 pKa = 3.76SGGAVVVDD1423 pKa = 5.09PINNTWYY1430 pKa = 10.2LQLPGSLALATYY1442 pKa = 10.5DD1443 pKa = 2.76VTAQVRR1449 pKa = 11.84SSAGNGNEE1457 pKa = 3.74AGISHH1462 pKa = 7.03GSLVVYY1468 pKa = 10.8AEE1470 pKa = 4.31TAVDD1474 pKa = 3.76TSWASTAGATTTNAMAFSLNSSGLWNVVTGISDD1507 pKa = 3.74YY1508 pKa = 10.92TSSGLNSYY1516 pKa = 9.17TGTTLTNSRR1525 pKa = 11.84GNIVVSYY1532 pKa = 9.17TLADD1536 pKa = 3.41TDD1538 pKa = 4.46RR1539 pKa = 11.84NGTSDD1544 pKa = 3.48IYY1546 pKa = 10.88ATEE1549 pKa = 4.09TNYY1552 pKa = 10.92GGSIQGKK1559 pKa = 5.07WTANTDD1565 pKa = 3.0GTYY1568 pKa = 9.99TYY1570 pKa = 9.99GQQAIGTTIWYY1581 pKa = 8.91GGIMAYY1587 pKa = 10.51DD1588 pKa = 3.52KK1589 pKa = 11.34TGDD1592 pKa = 3.14GWLDD1596 pKa = 3.31MAYY1599 pKa = 10.36GDD1601 pKa = 4.42AGGDD1605 pKa = 3.48SRR1607 pKa = 11.84SYY1609 pKa = 10.84LVNNNGVLSPDD1620 pKa = 3.24GTYY1623 pKa = 10.89GAAGLAGVTYY1633 pKa = 10.64RR1634 pKa = 11.84EE1635 pKa = 3.96ISGVDD1640 pKa = 3.16INNDD1644 pKa = 3.02GTVDD1648 pKa = 3.53IVQHH1652 pKa = 5.26TNRR1655 pKa = 11.84SGAYY1659 pKa = 9.49ALTVINNSGTGALTEE1674 pKa = 4.23TQNIANVFVPNAANTTTAVSMTWADD1699 pKa = 3.73FNGDD1703 pKa = 3.75GLMDD1707 pKa = 4.68LYY1709 pKa = 10.51ISSGLNPNGSYY1720 pKa = 10.92NYY1722 pKa = 10.57GGAIYY1727 pKa = 10.11YY1728 pKa = 10.54NSGQGTLLTTPTAVEE1743 pKa = 4.02TSTTNTASAGYY1754 pKa = 10.14LSAAVDD1760 pKa = 3.42WDD1762 pKa = 3.7GDD1764 pKa = 3.42GDD1766 pKa = 3.68MDD1768 pKa = 4.67IIKK1771 pKa = 10.36FSTYY1775 pKa = 10.14SASQTAQLYY1784 pKa = 10.3VNDD1787 pKa = 4.29GTGKK1791 pKa = 9.29GWSSSTLASSLVNVTGIATLDD1812 pKa = 3.71YY1813 pKa = 10.65DD1814 pKa = 3.91WNGTQDD1820 pKa = 4.33LLVSQANGKK1829 pKa = 8.53VVYY1832 pKa = 9.64IANNASIADD1841 pKa = 3.71GTALHH1846 pKa = 6.63LRR1848 pKa = 11.84ILDD1851 pKa = 3.84GEE1853 pKa = 4.51GMNVFYY1859 pKa = 11.09GNTVQLYY1866 pKa = 8.28NSAGEE1871 pKa = 4.15RR1872 pKa = 11.84VASQIINAQSGVGVNDD1888 pKa = 3.39SSALVNFYY1896 pKa = 11.19GLDD1899 pKa = 3.56PNEE1902 pKa = 4.34TYY1904 pKa = 10.86SAVLVRR1910 pKa = 11.84NINGVSTNATWTDD1923 pKa = 3.51LSAGDD1928 pKa = 3.99GTSSYY1933 pKa = 11.59SLTADD1938 pKa = 3.32AANGGTSGTLTGTGYY1953 pKa = 11.1NDD1955 pKa = 3.23TFIAEE1960 pKa = 4.28SGTYY1964 pKa = 9.7VYY1966 pKa = 10.92NGGGGWSSSSDD1977 pKa = 3.18HH1978 pKa = 6.27ATWSATGGQDD1988 pKa = 3.93IVDD1991 pKa = 4.69FKK1993 pKa = 11.31NATSGVTVDD2002 pKa = 4.83LSSSSAQNTGFNTATFKK2019 pKa = 10.88DD2020 pKa = 3.41IEE2022 pKa = 4.88GIAGSNYY2029 pKa = 10.14DD2030 pKa = 4.19DD2031 pKa = 5.26VITGSAGNNVFEE2043 pKa = 4.6GRR2045 pKa = 11.84GGNDD2049 pKa = 2.93TFNLGKK2055 pKa = 10.55GGHH2058 pKa = 6.33DD2059 pKa = 3.13TLLYY2063 pKa = 10.65KK2064 pKa = 10.98LLDD2067 pKa = 3.91SSDD2070 pKa = 3.4ATGGNGVDD2078 pKa = 3.96TINGFKK2084 pKa = 10.54VGTWEE2089 pKa = 4.06GTANTDD2095 pKa = 4.46RR2096 pKa = 11.84LDD2098 pKa = 4.97LSDD2101 pKa = 5.11LLQSSGYY2108 pKa = 10.39SGTADD2113 pKa = 2.88ASYY2116 pKa = 11.32VNGVATLGSGVGNLANYY2133 pKa = 10.41LSVTTEE2139 pKa = 4.01GNNTVIKK2146 pKa = 10.64VDD2148 pKa = 3.94LDD2150 pKa = 3.84GSGTAQSMTTIATLTNVHH2168 pKa = 6.42TDD2170 pKa = 3.4LATLLANHH2178 pKa = 5.99QLVVGG2183 pKa = 4.31

Molecular weight: 221.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J8YI28|A0A0J8YI28_9GAMM Lysophospholipid transporter LplT OS=bacteria symbiont BFo1 of Frankliniella occidentalis OX=1628855 GN=lplT PE=3 SV=1
MM1 pKa = 7.11KK2 pKa = 9.36THH4 pKa = 6.38NLSGFTLLEE13 pKa = 3.9MMVVVTLVSLLSLGGWHH30 pKa = 6.91GWNSFQQRR38 pKa = 11.84QQLNDD43 pKa = 3.26SALQIQRR50 pKa = 11.84LLQRR54 pKa = 11.84LRR56 pKa = 11.84SDD58 pKa = 4.37ANWHH62 pKa = 5.37NRR64 pKa = 11.84TRR66 pKa = 11.84LLWLKK71 pKa = 10.41PGAGWCLGAGEE82 pKa = 4.65GAQPCSTPGPLHH94 pKa = 6.58LRR96 pKa = 11.84APYY99 pKa = 9.75PGIVVQTLTDD109 pKa = 3.2GMGFYY114 pKa = 10.62GKK116 pKa = 10.36KK117 pKa = 9.3NAARR121 pKa = 11.84PGRR124 pKa = 11.84IVIAGEE130 pKa = 3.59AGEE133 pKa = 4.03RR134 pKa = 11.84RR135 pKa = 11.84IIVSARR141 pKa = 11.84GRR143 pKa = 11.84VRR145 pKa = 11.84ICAQDD150 pKa = 3.53SEE152 pKa = 4.71EE153 pKa = 4.18CRR155 pKa = 3.62

Molecular weight: 17.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4649

0

4649

1442933

31

3363

310.4

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.321 ± 0.042

1.018 ± 0.013

5.159 ± 0.028

5.411 ± 0.035

3.735 ± 0.028

7.521 ± 0.038

2.218 ± 0.018

5.459 ± 0.033

3.972 ± 0.033

11.051 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.019

3.576 ± 0.026

4.512 ± 0.027

4.89 ± 0.038

5.804 ± 0.034

6.31 ± 0.033

5.172 ± 0.03

6.975 ± 0.03

1.517 ± 0.017

2.685 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski