Amino acid dipepetide frequency for Thermoactinomyces sp. DSM 45891

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.279AlaAla: 4.279 ± 0.09	0.662AlaCys: 0.662 ± 0.029	3.377AlaAsp: 3.377 ± 0.066	4.403AlaGlu: 4.403 ± 0.081	2.791AlaPhe: 2.791 ± 0.058	4.946AlaGly: 4.946 ± 0.091	1.461AlaHis: 1.461 ± 0.043	5.432AlaIle: 5.432 ± 0.086	4.293AlaLys: 4.293 ± 0.081	6.601AlaLeu: 6.601 ± 0.093	1.946AlaMet: 1.946 ± 0.054	2.552AlaAsn: 2.552 ± 0.055	2.179AlaPro: 2.179 ± 0.054	2.439AlaGln: 2.439 ± 0.055	3.17AlaArg: 3.17 ± 0.067	4.05AlaSer: 4.05 ± 0.07	3.988AlaThr: 3.988 ± 0.085	4.908AlaVal: 4.908 ± 0.084	0.731AlaTrp: 0.731 ± 0.029	2.239AlaTyr: 2.239 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.48CysAla: 0.48 ± 0.031	0.113CysCys: 0.113 ± 0.011	0.446CysAsp: 0.446 ± 0.022	0.493CysGlu: 0.493 ± 0.025	0.408CysPhe: 0.408 ± 0.022	0.753CysGly: 0.753 ± 0.028	0.21CysHis: 0.21 ± 0.017	0.629CysIle: 0.629 ± 0.029	0.458CysLys: 0.458 ± 0.026	0.824CysLeu: 0.824 ± 0.036	0.259CysMet: 0.259 ± 0.017	0.319CysAsn: 0.319 ± 0.018	0.404CysPro: 0.404 ± 0.022	0.368CysGln: 0.368 ± 0.023	0.39CysArg: 0.39 ± 0.023	0.596CysSer: 0.596 ± 0.029	0.509CysThr: 0.509 ± 0.022	0.553CysVal: 0.553 ± 0.029	0.105CysTrp: 0.105 ± 0.013	0.322CysTyr: 0.322 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.128AspAla: 3.128 ± 0.069	0.419AspCys: 0.419 ± 0.024	2.372AspAsp: 2.372 ± 0.055	3.882AspGlu: 3.882 ± 0.083	2.326AspPhe: 2.326 ± 0.063	3.602AspGly: 3.602 ± 0.08	1.386AspHis: 1.386 ± 0.043	3.312AspIle: 3.312 ± 0.067	2.547AspLys: 2.547 ± 0.065	5.721AspLeu: 5.721 ± 0.089	1.117AspMet: 1.117 ± 0.039	1.406AspAsn: 1.406 ± 0.046	2.502AspPro: 2.502 ± 0.061	2.707AspGln: 2.707 ± 0.064	2.867AspArg: 2.867 ± 0.061	2.878AspSer: 2.878 ± 0.064	2.549AspThr: 2.549 ± 0.064	3.651AspVal: 3.651 ± 0.067	0.773AspTrp: 0.773 ± 0.031	1.889AspTyr: 1.889 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.858GluAla: 4.858 ± 0.08	0.542GluCys: 0.542 ± 0.022	3.275GluAsp: 3.275 ± 0.064	6.077GluGlu: 6.077 ± 0.109	2.359GluPhe: 2.359 ± 0.055	4.035GluGly: 4.035 ± 0.059	1.453GluHis: 1.453 ± 0.039	5.438GluIle: 5.438 ± 0.088	5.53GluLys: 5.53 ± 0.088	6.763GluLeu: 6.763 ± 0.093	2.298GluMet: 2.298 ± 0.054	2.704GluAsn: 2.704 ± 0.055	2.036GluPro: 2.036 ± 0.054	3.694GluGln: 3.694 ± 0.069	3.861GluArg: 3.861 ± 0.067	3.77GluSer: 3.77 ± 0.076	3.329GluThr: 3.329 ± 0.059	5.159GluVal: 5.159 ± 0.091	1.036GluTrp: 1.036 ± 0.035	2.24GluTyr: 2.24 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	2.794PheAla: 2.794 ± 0.055	0.42PheCys: 0.42 ± 0.021	2.307PheAsp: 2.307 ± 0.056	2.529PheGlu: 2.529 ± 0.057	2.082PhePhe: 2.082 ± 0.071	3.088PheGly: 3.088 ± 0.067	1.13PheHis: 1.13 ± 0.034	2.969PheIle: 2.969 ± 0.072	1.567PheLys: 1.567 ± 0.047	4.318PheLeu: 4.318 ± 0.083	1.039PheMet: 1.039 ± 0.038	1.312PheAsn: 1.312 ± 0.041	1.773PhePro: 1.773 ± 0.049	1.947PheGln: 1.947 ± 0.053	1.876PheArg: 1.876 ± 0.046	2.993PheSer: 2.993 ± 0.078	2.558PheThr: 2.558 ± 0.063	3.085PheVal: 3.085 ± 0.063	0.474PheTrp: 0.474 ± 0.025	1.522PheTyr: 1.522 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.991GlyAla: 4.991 ± 0.108	0.687GlyCys: 0.687 ± 0.031	3.528GlyAsp: 3.528 ± 0.074	4.529GlyGlu: 4.529 ± 0.072	3.185GlyPhe: 3.185 ± 0.074	4.846GlyGly: 4.846 ± 0.105	1.432GlyHis: 1.432 ± 0.041	5.617GlyIle: 5.617 ± 0.105	4.849GlyLys: 4.849 ± 0.087	6.473GlyLeu: 6.473 ± 0.086	2.105GlyMet: 2.105 ± 0.052	2.805GlyAsn: 2.805 ± 0.091	2.842GlyPro: 2.842 ± 0.19	2.265GlyGln: 2.265 ± 0.057	3.073GlyArg: 3.073 ± 0.068	4.46GlySer: 4.46 ± 0.077	4.265GlyThr: 4.265 ± 0.103	5.519GlyVal: 5.519 ± 0.095	0.91GlyTrp: 0.91 ± 0.039	2.538GlyTyr: 2.538 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.401HisAla: 1.401 ± 0.044	0.213HisCys: 0.213 ± 0.015	1.067HisAsp: 1.067 ± 0.028	1.421HisGlu: 1.421 ± 0.042	1.147HisPhe: 1.147 ± 0.04	1.487HisGly: 1.487 ± 0.041	0.768HisHis: 0.768 ± 0.034	1.64HisIle: 1.64 ± 0.043	1.1HisLys: 1.1 ± 0.038	2.402HisLeu: 2.402 ± 0.055	0.548HisMet: 0.548 ± 0.026	0.722HisAsn: 0.722 ± 0.029	1.333HisPro: 1.333 ± 0.036	1.158HisGln: 1.158 ± 0.039	1.213HisArg: 1.213 ± 0.04	1.528HisSer: 1.528 ± 0.044	1.193HisThr: 1.193 ± 0.037	1.591HisVal: 1.591 ± 0.047	0.271HisTrp: 0.271 ± 0.019	0.935HisTyr: 0.935 ± 0.033	0.001HisXaa: 0.001 ± 0.001
Ile	5.56IleAla: 5.56 ± 0.089	0.763IleCys: 0.763 ± 0.036	3.855IleAsp: 3.855 ± 0.07	5.059IleGlu: 5.059 ± 0.077	2.668IlePhe: 2.668 ± 0.065	5.558IleGly: 5.558 ± 0.097	2.007IleHis: 2.007 ± 0.054	4.353IleIle: 4.353 ± 0.087	3.448IleLys: 3.448 ± 0.067	6.645IleLeu: 6.645 ± 0.098	1.543IleMet: 1.543 ± 0.045	2.438IleAsn: 2.438 ± 0.053	3.587IlePro: 3.587 ± 0.066	3.657IleGln: 3.657 ± 0.073	3.871IleArg: 3.871 ± 0.072	4.887IleSer: 4.887 ± 0.085	4.232IleThr: 4.232 ± 0.076	4.942IleVal: 4.942 ± 0.085	0.788IleTrp: 0.788 ± 0.03	2.362IleTyr: 2.362 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	3.996LysAla: 3.996 ± 0.071	0.406LysCys: 0.406 ± 0.023	3.137LysAsp: 3.137 ± 0.079	5.443LysGlu: 5.443 ± 0.099	1.622LysPhe: 1.622 ± 0.05	4.072LysGly: 4.072 ± 0.07	1.215LysHis: 1.215 ± 0.04	3.97LysIle: 3.97 ± 0.076	5.523LysLys: 5.523 ± 0.119	5.115LysLeu: 5.115 ± 0.08	2.008LysMet: 2.008 ± 0.046	2.421LysAsn: 2.421 ± 0.056	2.347LysPro: 2.347 ± 0.059	3.133LysGln: 3.133 ± 0.06	3.546LysArg: 3.546 ± 0.068	3.429LysSer: 3.429 ± 0.063	3.047LysThr: 3.047 ± 0.065	4.565LysVal: 4.565 ± 0.079	0.879LysTrp: 0.879 ± 0.037	1.875LysTyr: 1.875 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	7.281LeuAla: 7.281 ± 0.098	0.9LeuCys: 0.9 ± 0.038	5.104LeuAsp: 5.104 ± 0.083	6.693LeuGlu: 6.693 ± 0.096	4.448LeuPhe: 4.448 ± 0.089	6.633LeuGly: 6.633 ± 0.092	2.257LeuHis: 2.257 ± 0.059	6.324LeuIle: 6.324 ± 0.094	5.196LeuLys: 5.196 ± 0.085	9.661LeuLeu: 9.661 ± 0.128	2.395LeuMet: 2.395 ± 0.054	3.373LeuAsn: 3.373 ± 0.072	4.132LeuPro: 4.132 ± 0.077	4.371LeuGln: 4.371 ± 0.08	4.382LeuArg: 4.382 ± 0.073	7.222LeuSer: 7.222 ± 0.117	5.421LeuThr: 5.421 ± 0.079	6.753LeuVal: 6.753 ± 0.093	0.93LeuTrp: 0.93 ± 0.033	3.269LeuTyr: 3.269 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	1.806MetAla: 1.806 ± 0.054	0.185MetCys: 0.185 ± 0.015	1.568MetAsp: 1.568 ± 0.043	1.989MetGlu: 1.989 ± 0.049	0.925MetPhe: 0.925 ± 0.034	1.973MetGly: 1.973 ± 0.05	0.434MetHis: 0.434 ± 0.023	2.315MetIle: 2.315 ± 0.06	2.384MetLys: 2.384 ± 0.055	2.328MetLeu: 2.328 ± 0.054	0.975MetMet: 0.975 ± 0.036	1.428MetAsn: 1.428 ± 0.041	0.841MetPro: 0.841 ± 0.033	0.911MetGln: 0.911 ± 0.036	1.249MetArg: 1.249 ± 0.04	1.839MetSer: 1.839 ± 0.048	1.449MetThr: 1.449 ± 0.038	1.936MetVal: 1.936 ± 0.046	0.231MetTrp: 0.231 ± 0.017	0.719MetTyr: 0.719 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	2.219AsnAla: 2.219 ± 0.051	0.304AsnCys: 0.304 ± 0.021	1.657AsnAsp: 1.657 ± 0.05	2.57AsnGlu: 2.57 ± 0.052	1.304AsnPhe: 1.304 ± 0.04	2.76AsnGly: 2.76 ± 0.077	1.057AsnHis: 1.057 ± 0.031	2.537AsnIle: 2.537 ± 0.065	2.181AsnLys: 2.181 ± 0.058	3.445AsnLeu: 3.445 ± 0.063	0.941AsnMet: 0.941 ± 0.033	1.385AsnAsn: 1.385 ± 0.047	2.169AsnPro: 2.169 ± 0.056	2.411AsnGln: 2.411 ± 0.051	2.145AsnArg: 2.145 ± 0.052	2.104AsnSer: 2.104 ± 0.058	2.105AsnThr: 2.105 ± 0.076	2.592AsnVal: 2.592 ± 0.058	0.548AsnTrp: 0.548 ± 0.027	1.191AsnTyr: 1.191 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.372ProAla: 2.372 ± 0.059	0.282ProCys: 0.282 ± 0.018	2.383ProAsp: 2.383 ± 0.054	3.128ProGlu: 3.128 ± 0.058	1.958ProPhe: 1.958 ± 0.048	2.433ProGly: 2.433 ± 0.059	0.944ProHis: 0.944 ± 0.036	3.05ProIle: 3.05 ± 0.066	2.264ProLys: 2.264 ± 0.061	3.571ProLeu: 3.571 ± 0.068	0.924ProMet: 0.924 ± 0.033	1.801ProAsn: 1.801 ± 0.05	1.224ProPro: 1.224 ± 0.043	2.004ProGln: 2.004 ± 0.118	1.538ProArg: 1.538 ± 0.043	2.767ProSer: 2.767 ± 0.059	2.699ProThr: 2.699 ± 0.072	2.982ProVal: 2.982 ± 0.064	0.444ProTrp: 0.444 ± 0.024	1.552ProTyr: 1.552 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.215GlnAla: 3.215 ± 0.065	0.269GlnCys: 0.269 ± 0.019	2.119GlnAsp: 2.119 ± 0.054	3.307GlnGlu: 3.307 ± 0.07	1.72GlnPhe: 1.72 ± 0.046	3.499GlnGly: 3.499 ± 0.135	0.883GlnHis: 0.883 ± 0.03	3.32GlnIle: 3.32 ± 0.07	3.096GlnLys: 3.096 ± 0.072	4.441GlnLeu: 4.441 ± 0.074	1.404GlnMet: 1.404 ± 0.043	1.695GlnAsn: 1.695 ± 0.043	1.457GlnPro: 1.457 ± 0.038	1.971GlnGln: 1.971 ± 0.06	1.869GlnArg: 1.869 ± 0.052	2.602GlnSer: 2.602 ± 0.059	2.286GlnThr: 2.286 ± 0.056	3.539GlnVal: 3.539 ± 0.074	0.47GlnTrp: 0.47 ± 0.028	1.513GlnTyr: 1.513 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.865ArgAla: 2.865 ± 0.059	0.389ArgCys: 0.389 ± 0.024	2.524ArgAsp: 2.524 ± 0.048	3.829ArgGlu: 3.829 ± 0.071	2.327ArgPhe: 2.327 ± 0.051	2.893ArgGly: 2.893 ± 0.058	0.933ArgHis: 0.933 ± 0.037	3.564ArgIle: 3.564 ± 0.062	3.379ArgLys: 3.379 ± 0.065	4.893ArgLeu: 4.893 ± 0.082	1.596ArgMet: 1.596 ± 0.044	2.045ArgAsn: 2.045 ± 0.052	1.703ArgPro: 1.703 ± 0.043	1.897ArgGln: 1.897 ± 0.046	2.394ArgArg: 2.394 ± 0.069	2.871ArgSer: 2.871 ± 0.053	2.468ArgThr: 2.468 ± 0.052	3.536ArgVal: 3.536 ± 0.063	0.626ArgTrp: 0.626 ± 0.027	1.918ArgTyr: 1.918 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	3.746SerAla: 3.746 ± 0.071	0.507SerCys: 0.507 ± 0.022	3.016SerAsp: 3.016 ± 0.065	3.673SerGlu: 3.673 ± 0.065	3.194SerPhe: 3.194 ± 0.072	4.739SerGly: 4.739 ± 0.076	1.519SerHis: 1.519 ± 0.045	4.937SerIle: 4.937 ± 0.074	3.941SerLys: 3.941 ± 0.072	6.446SerLeu: 6.446 ± 0.102	1.88SerMet: 1.88 ± 0.048	2.469SerAsn: 2.469 ± 0.063	2.433SerPro: 2.433 ± 0.061	2.449SerGln: 2.449 ± 0.049	2.947SerArg: 2.947 ± 0.058	4.444SerSer: 4.444 ± 0.084	3.64SerThr: 3.64 ± 0.07	4.393SerVal: 4.393 ± 0.069	0.786SerTrp: 0.786 ± 0.032	2.273SerTyr: 2.273 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	3.504ThrAla: 3.504 ± 0.063	0.48ThrCys: 0.48 ± 0.025	2.784ThrAsp: 2.784 ± 0.067	3.398ThrGlu: 3.398 ± 0.066	2.408ThrPhe: 2.408 ± 0.058	5.338ThrGly: 5.338 ± 0.195	1.289ThrHis: 1.289 ± 0.037	4.23ThrIle: 4.23 ± 0.075	3.14ThrLys: 3.14 ± 0.068	5.259ThrLeu: 5.259 ± 0.073	1.347ThrMet: 1.347 ± 0.041	2.194ThrAsn: 2.194 ± 0.059	2.687ThrPro: 2.687 ± 0.06	1.891ThrGln: 1.891 ± 0.049	2.255ThrArg: 2.255 ± 0.049	3.518ThrSer: 3.518 ± 0.06	3.304ThrThr: 3.304 ± 0.079	3.981ThrVal: 3.981 ± 0.081	0.691ThrTrp: 0.691 ± 0.033	2.049ThrTyr: 2.049 ± 0.073	0.002ThrXaa: 0.002 ± 0.002
Val	5.248ValAla: 5.248 ± 0.092	0.678ValCys: 0.678 ± 0.028	3.884ValAsp: 3.884 ± 0.064	5.175ValGlu: 5.175 ± 0.076	2.855ValPhe: 2.855 ± 0.061	5.248ValGly: 5.248 ± 0.093	1.613ValHis: 1.613 ± 0.046	5.216ValIle: 5.216 ± 0.097	4.292ValLys: 4.292 ± 0.081	6.71ValLeu: 6.71 ± 0.09	1.936ValMet: 1.936 ± 0.051	2.685ValAsn: 2.685 ± 0.062	2.957ValPro: 2.957 ± 0.062	2.919ValGln: 2.919 ± 0.058	3.519ValArg: 3.519 ± 0.067	4.694ValSer: 4.694 ± 0.082	4.23ValThr: 4.23 ± 0.079	5.572ValVal: 5.572 ± 0.097	0.737ValTrp: 0.737 ± 0.032	2.283ValTyr: 2.283 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.645TrpAla: 0.645 ± 0.027	0.121TrpCys: 0.121 ± 0.011	0.689TrpAsp: 0.689 ± 0.033	0.755TrpGlu: 0.755 ± 0.031	0.535TrpPhe: 0.535 ± 0.025	0.698TrpGly: 0.698 ± 0.031	0.22TrpHis: 0.22 ± 0.018	1.148TrpIle: 1.148 ± 0.044	0.899TrpLys: 0.899 ± 0.034	1.368TrpLeu: 1.368 ± 0.047	0.457TrpMet: 0.457 ± 0.024	0.624TrpAsn: 0.624 ± 0.034	0.24TrpPro: 0.24 ± 0.02	0.435TrpGln: 0.435 ± 0.023	0.507TrpArg: 0.507 ± 0.024	0.729TrpSer: 0.729 ± 0.031	0.613TrpThr: 0.613 ± 0.029	0.781TrpVal: 0.781 ± 0.032	0.183TrpTrp: 0.183 ± 0.014	0.375TrpTyr: 0.375 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.06TyrAla: 2.06 ± 0.051	0.296TyrCys: 0.296 ± 0.02	1.943TyrAsp: 1.943 ± 0.08	2.105TyrGlu: 2.105 ± 0.051	1.519TyrPhe: 1.519 ± 0.045	2.321TyrGly: 2.321 ± 0.052	0.928TyrHis: 0.928 ± 0.034	2.225TyrIle: 2.225 ± 0.05	1.663TyrLys: 1.663 ± 0.052	3.76TyrLeu: 3.76 ± 0.067	0.763TyrMet: 0.763 ± 0.034	1.249TyrAsn: 1.249 ± 0.05	1.475TyrPro: 1.475 ± 0.046	2.046TyrGln: 2.046 ± 0.049	1.976TyrArg: 1.976 ± 0.049	2.051TyrSer: 2.051 ± 0.051	1.863TyrThr: 1.863 ± 0.053	2.412TyrVal: 2.412 ± 0.049	0.408TyrTrp: 0.408 ± 0.021	1.356TyrTyr: 1.356 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.03XaaXaa: 0.03 ± 0.018

Statistics based on 2915 proteins (868414 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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