Hubei tombus-like virus 30

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG52|A0A1L3KG52_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 30 OX=1923278 PE=4 SV=1
MM1 pKa = 7.54ANSRR5 pKa = 11.84NNDD8 pKa = 3.42PATPGLIHH16 pKa = 7.71KK17 pKa = 7.63MITYY21 pKa = 9.51LFSKK25 pKa = 10.42DD26 pKa = 3.51EE27 pKa = 4.55PEE29 pKa = 4.4TIEE32 pKa = 5.85DD33 pKa = 3.94DD34 pKa = 4.63LEE36 pKa = 5.71SITNAAINLVSLQNSLGEE54 pKa = 4.17IKK56 pKa = 10.64AGATLSEE63 pKa = 4.49VTRR66 pKa = 11.84QEE68 pKa = 4.29QILEE72 pKa = 4.2KK73 pKa = 10.13KK74 pKa = 10.51NKK76 pKa = 9.72IIEE79 pKa = 4.14LLEE82 pKa = 4.25VNDD85 pKa = 4.0QKK87 pKa = 11.58NLEE90 pKa = 4.1PSYY93 pKa = 9.93EE94 pKa = 3.89EE95 pKa = 3.49CRR97 pKa = 11.84AYY99 pKa = 9.84IEE101 pKa = 4.09NRR103 pKa = 11.84VTGKK107 pKa = 10.66LVDD110 pKa = 3.5NTYY113 pKa = 11.01LAFCAALARR122 pKa = 11.84QYY124 pKa = 11.39LSTRR128 pKa = 11.84KK129 pKa = 9.3QVTEE133 pKa = 4.41RR134 pKa = 11.84SANRR138 pKa = 11.84IVQTVLQDD146 pKa = 3.32HH147 pKa = 6.97LEE149 pKa = 4.56GIRR152 pKa = 11.84PKK154 pKa = 10.15WNYY157 pKa = 10.53NIYY160 pKa = 10.76NILKK164 pKa = 9.79HH165 pKa = 6.13NDD167 pKa = 3.39NIGFAEE173 pKa = 3.86NMYY176 pKa = 10.57LKK178 pKa = 10.38FGRR181 pKa = 11.84WVMHH185 pKa = 6.24IPILSWSLPEE195 pKa = 3.85NAQRR199 pKa = 11.84PGITGHH205 pKa = 6.16PFRR208 pKa = 11.84YY209 pKa = 9.51LLSILATLALLYY221 pKa = 10.15ICLSGGSSTAHH232 pKa = 6.11QSTTPAPSITIPNAPPPPLPPLKK255 pKa = 10.57SDD257 pKa = 3.65TPPLNSMSTDD267 pKa = 3.31QNTLSHH273 pKa = 7.33LLTFGSSIANQVSGAVMSTYY293 pKa = 10.72HH294 pKa = 7.62KK295 pKa = 10.16LTTPQKK301 pKa = 9.63EE302 pKa = 4.11NTIKK306 pKa = 10.68EE307 pKa = 4.13LLISLGLVEE316 pKa = 6.07RR317 pKa = 11.84SQPWYY322 pKa = 10.86VRR324 pKa = 11.84LLSWKK329 pKa = 10.25NIAQASTKK337 pKa = 10.62LQDD340 pKa = 3.25SYY342 pKa = 11.25KK343 pKa = 10.39QEE345 pKa = 3.6IHH347 pKa = 6.66PSILSTVDD355 pKa = 2.92TLNQLKK361 pKa = 10.26EE362 pKa = 4.22PLKK365 pKa = 9.68QTYY368 pKa = 8.63TSAKK372 pKa = 9.16EE373 pKa = 4.11HH374 pKa = 5.49TTSAAQKK381 pKa = 10.44LKK383 pKa = 11.14DD384 pKa = 3.75SVANGAGTLSATIPPLMHH402 pKa = 6.7TSRR405 pKa = 11.84SKK407 pKa = 10.26CSSYY411 pKa = 10.11ATSFIEE417 pKa = 4.23NAKK420 pKa = 9.13DD421 pKa = 3.84TIRR424 pKa = 11.84IWNPWRR430 pKa = 11.84AEE432 pKa = 3.84PFTTRR437 pKa = 11.84VRR439 pKa = 11.84RR440 pKa = 11.84ATVSATKK447 pKa = 10.56SLGHH451 pKa = 6.62GCLAMLTLVWEE462 pKa = 4.68TVSSITTYY470 pKa = 10.94LYY472 pKa = 10.35TLLDD476 pKa = 3.6SSVYY480 pKa = 10.75EE481 pKa = 4.0GMPLSMEE488 pKa = 4.27TTASSSQTNPSQPTSTSKK506 pKa = 10.77FFDD509 pKa = 3.46SLIWKK514 pKa = 9.6ALFFLRR520 pKa = 11.84SIIFIKK526 pKa = 10.72LSFAVLGLSTIRR538 pKa = 11.84TRR540 pKa = 11.84TRR542 pKa = 11.84LLCLIPLACVVYY554 pKa = 9.11MVV556 pKa = 4.16

Molecular weight: 61.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG52|A0A1L3KG52_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 30 OX=1923278 PE=4 SV=1
MM1 pKa = 7.67LEE3 pKa = 3.96RR4 pKa = 11.84RR5 pKa = 11.84IQYY8 pKa = 9.94RR9 pKa = 11.84PSIYY13 pKa = 10.52NPGSFYY19 pKa = 11.01YY20 pKa = 10.59YY21 pKa = 8.44PQCSATTVAAIEE33 pKa = 4.2IRR35 pKa = 11.84HH36 pKa = 6.1APVKK40 pKa = 10.16LDD42 pKa = 3.51EE43 pKa = 4.12YY44 pKa = 10.66RR45 pKa = 11.84PEE47 pKa = 4.03HH48 pKa = 6.17FKK50 pKa = 11.3SLTHH54 pKa = 7.06LRR56 pKa = 11.84QFYY59 pKa = 9.78CKK61 pKa = 9.67PGVWSRR67 pKa = 11.84DD68 pKa = 3.35EE69 pKa = 4.61YY70 pKa = 10.78ISQIDD75 pKa = 3.69DD76 pKa = 3.57PAKK79 pKa = 8.97RR80 pKa = 11.84KK81 pKa = 9.78YY82 pKa = 7.48YY83 pKa = 10.51QRR85 pKa = 11.84VADD88 pKa = 3.55QLGFGRR94 pKa = 11.84KK95 pKa = 7.78VSALVCAFTKK105 pKa = 10.56LEE107 pKa = 4.29KK108 pKa = 10.95YY109 pKa = 8.51STSKK113 pKa = 10.95YY114 pKa = 9.36KK115 pKa = 10.66APRR118 pKa = 11.84LIQARR123 pKa = 11.84DD124 pKa = 3.17PSFNLEE130 pKa = 3.63YY131 pKa = 10.84GRR133 pKa = 11.84YY134 pKa = 8.32IKK136 pKa = 10.42PIEE139 pKa = 4.26RR140 pKa = 11.84ALKK143 pKa = 9.09TNVHH147 pKa = 6.5FGKK150 pKa = 9.0GTYY153 pKa = 9.36DD154 pKa = 2.71ICGAKK159 pKa = 9.38IEE161 pKa = 4.18RR162 pKa = 11.84LRR164 pKa = 11.84SKK166 pKa = 9.18WRR168 pKa = 11.84WYY170 pKa = 8.46TEE172 pKa = 4.15CDD174 pKa = 3.12HH175 pKa = 6.51STFDD179 pKa = 3.24AHH181 pKa = 5.85VTVEE185 pKa = 4.0MLKK188 pKa = 10.46LCHH191 pKa = 5.98QFYY194 pKa = 10.64RR195 pKa = 11.84KK196 pKa = 9.55CQGHH200 pKa = 5.53NPYY203 pKa = 9.26MEE205 pKa = 4.24SLARR209 pKa = 11.84RR210 pKa = 11.84TIHH213 pKa = 6.48NKK215 pKa = 7.83GTTRR219 pKa = 11.84YY220 pKa = 8.84GEE222 pKa = 4.21RR223 pKa = 11.84YY224 pKa = 8.91KK225 pKa = 10.46VTGTRR230 pKa = 11.84MSGDD234 pKa = 3.1VDD236 pKa = 3.63TSLGNSLINYY246 pKa = 7.97HH247 pKa = 6.36ILIHH251 pKa = 6.28ALRR254 pKa = 11.84LIGIRR259 pKa = 11.84GDD261 pKa = 3.88AIVNGDD267 pKa = 3.79DD268 pKa = 5.08SIIFTNEE275 pKa = 3.9PIPTDD280 pKa = 3.25KK281 pKa = 10.82YY282 pKa = 10.53VQILRR287 pKa = 11.84QFNMEE292 pKa = 4.4SVVLPSVNNIHH303 pKa = 6.74KK304 pKa = 10.63VEE306 pKa = 4.8FCRR309 pKa = 11.84TRR311 pKa = 11.84LIYY314 pKa = 10.68HH315 pKa = 7.02PDD317 pKa = 3.38KK318 pKa = 11.33NPTLMFDD325 pKa = 3.81PARR328 pKa = 11.84LRR330 pKa = 11.84SIYY333 pKa = 10.59GMTWKK338 pKa = 10.36SYY340 pKa = 9.42PDD342 pKa = 3.5HH343 pKa = 7.64VYY345 pKa = 10.69VKK347 pKa = 9.58YY348 pKa = 11.08LEE350 pKa = 4.69AVSHH354 pKa = 6.81ANSCMNSNSPVGRR367 pKa = 11.84EE368 pKa = 3.71WKK370 pKa = 10.17ALEE373 pKa = 5.06RR374 pKa = 11.84IGKK377 pKa = 8.1QLNLLEE383 pKa = 5.1RR384 pKa = 11.84NIQLEE389 pKa = 4.08LAKK392 pKa = 10.76QNGANRR398 pKa = 11.84PYY400 pKa = 9.74TWDD403 pKa = 3.57YY404 pKa = 10.84LDD406 pKa = 4.12PSITEE411 pKa = 4.6AYY413 pKa = 8.78PNYY416 pKa = 10.61RR417 pKa = 11.84MTDD420 pKa = 3.24KK421 pKa = 10.82QHH423 pKa = 5.75PTVPRR428 pKa = 11.84EE429 pKa = 4.11VTPLDD434 pKa = 3.35HH435 pKa = 7.37LINHH439 pKa = 5.79NTQEE443 pKa = 4.32LLLGPLGYY451 pKa = 10.87

Molecular weight: 52.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1007

451

556

503.5

57.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.157 ± 0.645

1.589 ± 0.248

3.774 ± 0.674

5.263 ± 0.036

2.681 ± 0.123

4.27 ± 0.507

2.979 ± 0.618

6.753 ± 0.197

5.76 ± 0.274

10.427 ± 1.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.986 ± 0.006

5.263 ± 0.1

5.76 ± 0.003

3.774 ± 0.138

5.958 ± 1.236

8.342 ± 1.573

8.044 ± 1.256

4.667 ± 0.264

1.49 ± 0.097

5.065 ± 1.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski