Fusarium sporotrichioides

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium sambucinum species complex

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A395RVZ6|A0A395RVZ6_FUSSP Necrosis inducing-domain-containing OS=Fusarium sporotrichioides OX=5514 GN=FSPOR_8307 PE=3 SV=1
MM1 pKa = 7.51GAQISVVTSNDD12 pKa = 2.83ATEE15 pKa = 3.92VANAIFNGPGVSVVGATLEE34 pKa = 3.98RR35 pKa = 11.84NYY37 pKa = 10.29WEE39 pKa = 4.65EE40 pKa = 3.79DD41 pKa = 3.43TLNGNRR47 pKa = 11.84GSVGTFTRR55 pKa = 11.84GPFGIGSGGILSSGWSRR72 pKa = 11.84DD73 pKa = 3.58AKK75 pKa = 10.86DD76 pKa = 3.59SSATGNRR83 pKa = 11.84DD84 pKa = 3.03TGVDD88 pKa = 3.38GSLYY92 pKa = 10.14CGPGSTTNGAVLSVDD107 pKa = 3.01ILVDD111 pKa = 3.24TGYY114 pKa = 11.17NGLSVEE120 pKa = 4.54FVLATDD126 pKa = 4.36EE127 pKa = 4.08NLQGNADD134 pKa = 3.57PMGIYY139 pKa = 10.31LDD141 pKa = 4.11DD142 pKa = 3.44VQYY145 pKa = 11.88ALDD148 pKa = 3.63TSGNRR153 pKa = 11.84ITVNSDD159 pKa = 3.08YY160 pKa = 10.6LQQPIGITDD169 pKa = 3.72NQLLSATMYY178 pKa = 11.19GNASPLLIMGLAASPGQHH196 pKa = 5.47RR197 pKa = 11.84MIFAICDD204 pKa = 3.51ANNGEE209 pKa = 4.44KK210 pKa = 10.59DD211 pKa = 3.32SALMVKK217 pKa = 10.49AGACVDD223 pKa = 3.71CVRR226 pKa = 11.84DD227 pKa = 3.49IKK229 pKa = 11.18LNYY232 pKa = 8.01ATTTTTVGPTPFVSTIKK249 pKa = 10.62AALTMSGTVVYY260 pKa = 9.11GVPEE264 pKa = 4.13PATTEE269 pKa = 3.95VTTTTEE275 pKa = 3.84EE276 pKa = 4.46AYY278 pKa = 9.37STTTADD284 pKa = 3.03ATSYY288 pKa = 10.63TEE290 pKa = 4.15EE291 pKa = 4.24TTSTTEE297 pKa = 3.76LMSMSEE303 pKa = 4.21EE304 pKa = 4.21STPTEE309 pKa = 3.84PTATADD315 pKa = 3.61VLSTSTEE322 pKa = 3.59ASTAAEE328 pKa = 4.14APTTNDD334 pKa = 2.92LPTTTGVSSEE344 pKa = 4.36EE345 pKa = 3.91LTSTDD350 pKa = 3.57EE351 pKa = 4.33SSTTADD357 pKa = 3.42YY358 pKa = 7.52TTTATTTTTYY368 pKa = 10.82NQGSEE373 pKa = 4.13TSSAEE378 pKa = 4.14EE379 pKa = 4.26PDD381 pKa = 3.56TTTTIATSEE390 pKa = 4.16TMTNSMDD397 pKa = 3.37VSEE400 pKa = 5.12SSVSTSMDD408 pKa = 3.03STLSNDD414 pKa = 3.33SALITAAATTTSTSQASMSTASTLTTIRR442 pKa = 11.84RR443 pKa = 11.84PCRR446 pKa = 11.84PP447 pKa = 3.24

Molecular weight: 46.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A395SPL0|A0A395SPL0_FUSSP S-adenosyl-L-methionine-dependent N-methyltransferase OS=Fusarium sporotrichioides OX=5514 GN=FSPOR_1714 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.18RR8 pKa = 11.84HH9 pKa = 4.47AAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APARR26 pKa = 11.84SNRR29 pKa = 11.84HH30 pKa = 4.32TVTTTTTTSKK40 pKa = 10.17PRR42 pKa = 11.84RR43 pKa = 11.84GMFGGGSTTRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 4.8GTTPVHH62 pKa = 5.36HH63 pKa = 6.39HH64 pKa = 5.26QRR66 pKa = 11.84RR67 pKa = 11.84PSMKK71 pKa = 10.02DD72 pKa = 2.95KK73 pKa = 11.41VSGALLKK80 pKa = 11.04LKK82 pKa = 10.68GSLTRR87 pKa = 11.84RR88 pKa = 11.84PGVKK92 pKa = 9.89AAGTRR97 pKa = 11.84RR98 pKa = 11.84MHH100 pKa = 5.65GTDD103 pKa = 2.99GRR105 pKa = 11.84GARR108 pKa = 11.84HH109 pKa = 5.87HH110 pKa = 7.23RR111 pKa = 11.84YY112 pKa = 9.44

Molecular weight: 12.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11960

0

11960

6079613

42

8933

508.3

56.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.327 ± 0.021

1.245 ± 0.009

5.94 ± 0.019

6.265 ± 0.026

3.773 ± 0.015

6.789 ± 0.022

2.335 ± 0.009

5.088 ± 0.017

5.091 ± 0.02

8.656 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.008

3.858 ± 0.011

5.922 ± 0.024

4.033 ± 0.018

5.754 ± 0.02

8.129 ± 0.022

6.092 ± 0.026

6.148 ± 0.018

1.511 ± 0.009

2.803 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski