Amino acid dipepetide frequency for Streptomyces sp. WAC04770

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.967AlaAla: 20.967 ± 0.13
1.079AlaCys: 1.079 ± 0.022
8.289AlaAsp: 8.289 ± 0.066
9.086AlaGlu: 9.086 ± 0.091
3.521AlaPhe: 3.521 ± 0.041
12.584AlaGly: 12.584 ± 0.092
2.869AlaHis: 2.869 ± 0.038
3.54AlaIle: 3.54 ± 0.048
2.895AlaLys: 2.895 ± 0.052
14.694AlaLeu: 14.694 ± 0.118
2.455AlaMet: 2.455 ± 0.033
1.864AlaAsn: 1.864 ± 0.035
7.217AlaPro: 7.217 ± 0.066
3.63AlaGln: 3.63 ± 0.049
10.136AlaArg: 10.136 ± 0.079
5.905AlaSer: 5.905 ± 0.057
7.117AlaThr: 7.117 ± 0.061
12.225AlaVal: 12.225 ± 0.082
1.757AlaTrp: 1.757 ± 0.031
2.807AlaTyr: 2.807 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.058CysAla: 1.058 ± 0.025
0.099CysCys: 0.099 ± 0.008
0.446CysAsp: 0.446 ± 0.017
0.402CysGlu: 0.402 ± 0.015
0.22CysPhe: 0.22 ± 0.011
0.964CysGly: 0.964 ± 0.022
0.177CysHis: 0.177 ± 0.009
0.223CysIle: 0.223 ± 0.011
0.114CysLys: 0.114 ± 0.008
0.761CysLeu: 0.761 ± 0.022
0.12CysMet: 0.12 ± 0.008
0.128CysAsn: 0.128 ± 0.008
0.494CysPro: 0.494 ± 0.017
0.161CysGln: 0.161 ± 0.01
0.602CysArg: 0.602 ± 0.018
0.441CysSer: 0.441 ± 0.014
0.542CysThr: 0.542 ± 0.016
0.637CysVal: 0.637 ± 0.019
0.124CysTrp: 0.124 ± 0.008
0.153CysTyr: 0.153 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.719AspAla: 7.719 ± 0.061
0.406AspCys: 0.406 ± 0.014
3.493AspAsp: 3.493 ± 0.044
3.96AspGlu: 3.96 ± 0.051
1.616AspPhe: 1.616 ± 0.032
6.498AspGly: 6.498 ± 0.063
1.49AspHis: 1.49 ± 0.028
1.955AspIle: 1.955 ± 0.033
1.144AspLys: 1.144 ± 0.029
5.984AspLeu: 5.984 ± 0.058
0.771AspMet: 0.771 ± 0.018
0.915AspAsn: 0.915 ± 0.021
4.551AspPro: 4.551 ± 0.046
1.466AspGln: 1.466 ± 0.025
5.199AspArg: 5.199 ± 0.059
2.507AspSer: 2.507 ± 0.034
3.189AspThr: 3.189 ± 0.041
4.531AspVal: 4.531 ± 0.053
0.975AspTrp: 0.975 ± 0.023
1.048AspTyr: 1.048 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.835GluAla: 7.835 ± 0.085
0.361GluCys: 0.361 ± 0.014
2.866GluAsp: 2.866 ± 0.04
3.883GluGlu: 3.883 ± 0.055
1.528GluPhe: 1.528 ± 0.025
4.738GluGly: 4.738 ± 0.054
1.46GluHis: 1.46 ± 0.025
2.362GluIle: 2.362 ± 0.04
1.718GluLys: 1.718 ± 0.034
7.025GluLeu: 7.025 ± 0.068
0.89GluMet: 0.89 ± 0.023
1.101GluAsn: 1.101 ± 0.023
3.471GluPro: 3.471 ± 0.043
2.229GluGln: 2.229 ± 0.033
5.743GluArg: 5.743 ± 0.056
2.631GluSer: 2.631 ± 0.037
3.053GluThr: 3.053 ± 0.037
4.55GluVal: 4.55 ± 0.051
0.805GluTrp: 0.805 ± 0.022
1.12GluTyr: 1.12 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.661PheAla: 3.661 ± 0.047
0.272PheCys: 0.272 ± 0.013
2.018PheAsp: 2.018 ± 0.033
1.45PheGlu: 1.45 ± 0.029
0.892PhePhe: 0.892 ± 0.021
3.028PheGly: 3.028 ± 0.044
0.607PheHis: 0.607 ± 0.014
0.782PheIle: 0.782 ± 0.019
0.509PheLys: 0.509 ± 0.017
2.577PheLeu: 2.577 ± 0.039
0.383PheMet: 0.383 ± 0.014
0.566PheAsn: 0.566 ± 0.016
1.397PhePro: 1.397 ± 0.029
0.659PheGln: 0.659 ± 0.02
1.843PheArg: 1.843 ± 0.032
1.475PheSer: 1.475 ± 0.027
2.101PheThr: 2.101 ± 0.033
2.169PheVal: 2.169 ± 0.033
0.415PheTrp: 0.415 ± 0.015
0.564PheTyr: 0.564 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.19GlyAla: 11.19 ± 0.092
0.841GlyCys: 0.841 ± 0.019
5.075GlyAsp: 5.075 ± 0.063
5.451GlyGlu: 5.451 ± 0.051
2.85GlyPhe: 2.85 ± 0.039
8.924GlyGly: 8.924 ± 0.095
2.28GlyHis: 2.28 ± 0.033
3.576GlyIle: 3.576 ± 0.045
2.572GlyLys: 2.572 ± 0.038
9.444GlyLeu: 9.444 ± 0.081
1.913GlyMet: 1.913 ± 0.03
1.808GlyAsn: 1.808 ± 0.036
5.393GlyPro: 5.393 ± 0.056
2.685GlyGln: 2.685 ± 0.037
7.856GlyArg: 7.856 ± 0.058
5.559GlySer: 5.559 ± 0.069
6.39GlyThr: 6.39 ± 0.068
7.182GlyVal: 7.182 ± 0.057
1.657GlyTrp: 1.657 ± 0.029
2.23GlyTyr: 2.23 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.624HisAla: 2.624 ± 0.034
0.218HisCys: 0.218 ± 0.009
1.322HisAsp: 1.322 ± 0.028
1.198HisGlu: 1.198 ± 0.023
0.63HisPhe: 0.63 ± 0.017
2.341HisGly: 2.341 ± 0.035
0.734HisHis: 0.734 ± 0.021
0.799HisIle: 0.799 ± 0.02
0.347HisLys: 0.347 ± 0.013
2.502HisLeu: 2.502 ± 0.039
0.331HisMet: 0.331 ± 0.012
0.356HisAsn: 0.356 ± 0.013
1.79HisPro: 1.79 ± 0.029
0.667HisGln: 0.667 ± 0.019
2.246HisArg: 2.246 ± 0.033
1.007HisSer: 1.007 ± 0.02
1.355HisThr: 1.355 ± 0.027
1.589HisVal: 1.589 ± 0.028
0.356HisTrp: 0.356 ± 0.013
0.458HisTyr: 0.458 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.952IleAla: 4.952 ± 0.053
0.304IleCys: 0.304 ± 0.013
2.345IleAsp: 2.345 ± 0.038
2.071IleGlu: 2.071 ± 0.034
0.738IlePhe: 0.738 ± 0.019
3.725IleGly: 3.725 ± 0.052
0.691IleHis: 0.691 ± 0.018
0.954IleIle: 0.954 ± 0.024
0.776IleLys: 0.776 ± 0.021
2.5IleLeu: 2.5 ± 0.038
0.503IleMet: 0.503 ± 0.015
0.722IleAsn: 0.722 ± 0.019
1.771IlePro: 1.771 ± 0.03
0.749IleGln: 0.749 ± 0.021
2.309IleArg: 2.309 ± 0.035
1.769IleSer: 1.769 ± 0.03
2.322IleThr: 2.322 ± 0.033
2.793IleVal: 2.793 ± 0.042
0.388IleTrp: 0.388 ± 0.014
0.534IleTyr: 0.534 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
3.056LysAla: 3.056 ± 0.047
0.104LysCys: 0.104 ± 0.007
1.392LysAsp: 1.392 ± 0.031
1.35LysGlu: 1.35 ± 0.029
0.509LysPhe: 0.509 ± 0.015
1.938LysGly: 1.938 ± 0.04
0.451LysHis: 0.451 ± 0.015
0.904LysIle: 0.904 ± 0.021
0.984LysLys: 0.984 ± 0.034
2.129LysLeu: 2.129 ± 0.038
0.379LysMet: 0.379 ± 0.014
0.571LysAsn: 0.571 ± 0.018
1.378LysPro: 1.378 ± 0.028
0.707LysGln: 0.707 ± 0.019
1.536LysArg: 1.536 ± 0.031
1.191LysSer: 1.191 ± 0.03
1.315LysThr: 1.315 ± 0.028
1.959LysVal: 1.959 ± 0.035
0.247LysTrp: 0.247 ± 0.011
0.441LysTyr: 0.441 ± 0.013
0.0LysXaa: 0.0 ± 0.0
Leu
14.88LeuAla: 14.88 ± 0.121
0.829LeuCys: 0.829 ± 0.023
6.71LeuAsp: 6.71 ± 0.068
4.928LeuGlu: 4.928 ± 0.054
2.636LeuPhe: 2.636 ± 0.038
9.136LeuGly: 9.136 ± 0.068
2.348LeuHis: 2.348 ± 0.034
3.503LeuIle: 3.503 ± 0.049
2.126LeuLys: 2.126 ± 0.04
11.418LeuLeu: 11.418 ± 0.108
1.682LeuMet: 1.682 ± 0.03
1.722LeuAsn: 1.722 ± 0.03
6.522LeuPro: 6.522 ± 0.063
2.143LeuGln: 2.143 ± 0.03
8.565LeuArg: 8.565 ± 0.08
5.387LeuSer: 5.387 ± 0.056
7.054LeuThr: 7.054 ± 0.063
8.658LeuVal: 8.658 ± 0.079
1.301LeuTrp: 1.301 ± 0.026
1.932LeuTyr: 1.932 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.27MetAla: 2.27 ± 0.03
0.17MetCys: 0.17 ± 0.009
0.87MetAsp: 0.87 ± 0.02
0.805MetGlu: 0.805 ± 0.017
0.46MetPhe: 0.46 ± 0.014
1.299MetGly: 1.299 ± 0.026
0.334MetHis: 0.334 ± 0.014
0.649MetIle: 0.649 ± 0.016
0.448MetLys: 0.448 ± 0.015
1.626MetLeu: 1.626 ± 0.026
0.301MetMet: 0.301 ± 0.013
0.432MetAsn: 0.432 ± 0.014
1.075MetPro: 1.075 ± 0.022
0.416MetGln: 0.416 ± 0.016
1.352MetArg: 1.352 ± 0.026
1.308MetSer: 1.308 ± 0.023
1.502MetThr: 1.502 ± 0.025
1.252MetVal: 1.252 ± 0.021
0.198MetTrp: 0.198 ± 0.01
0.327MetTyr: 0.327 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.225AsnAla: 2.225 ± 0.038
0.161AsnCys: 0.161 ± 0.009
0.948AsnAsp: 0.948 ± 0.023
0.836AsnGlu: 0.836 ± 0.022
0.491AsnPhe: 0.491 ± 0.014
1.921AsnGly: 1.921 ± 0.038
0.386AsnHis: 0.386 ± 0.013
0.656AsnIle: 0.656 ± 0.019
0.431AsnLys: 0.431 ± 0.015
1.632AsnLeu: 1.632 ± 0.031
0.308AsnMet: 0.308 ± 0.012
0.433AsnAsn: 0.433 ± 0.018
1.331AsnPro: 1.331 ± 0.03
0.522AsnGln: 0.522 ± 0.016
1.296AsnArg: 1.296 ± 0.024
0.935AsnSer: 0.935 ± 0.021
1.138AsnThr: 1.138 ± 0.024
1.343AsnVal: 1.343 ± 0.026
0.288AsnTrp: 0.288 ± 0.011
0.427AsnTyr: 0.427 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.395ProAla: 8.395 ± 0.064
0.336ProCys: 0.336 ± 0.014
4.58ProAsp: 4.58 ± 0.051
4.677ProGlu: 4.677 ± 0.052
1.596ProPhe: 1.596 ± 0.025
6.815ProGly: 6.815 ± 0.071
1.326ProHis: 1.326 ± 0.027
1.253ProIle: 1.253 ± 0.022
1.26ProLys: 1.26 ± 0.025
5.427ProLeu: 5.427 ± 0.064
0.983ProMet: 0.983 ± 0.024
0.87ProAsn: 0.87 ± 0.021
3.366ProPro: 3.366 ± 0.051
1.719ProGln: 1.719 ± 0.031
3.775ProArg: 3.775 ± 0.042
3.054ProSer: 3.054 ± 0.04
3.104ProThr: 3.104 ± 0.041
5.703ProVal: 5.703 ± 0.055
0.883ProTrp: 0.883 ± 0.021
1.463ProTyr: 1.463 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.534GlnAla: 3.534 ± 0.052
0.161GlnCys: 0.161 ± 0.008
1.347GlnAsp: 1.347 ± 0.023
1.592GlnGlu: 1.592 ± 0.027
0.645GlnPhe: 0.645 ± 0.017
2.273GlnGly: 2.273 ± 0.037
0.661GlnHis: 0.661 ± 0.022
1.076GlnIle: 1.076 ± 0.023
0.662GlnLys: 0.662 ± 0.021
3.083GlnLeu: 3.083 ± 0.039
0.465GlnMet: 0.465 ± 0.016
0.498GlnAsn: 0.498 ± 0.017
1.579GlnPro: 1.579 ± 0.032
1.223GlnGln: 1.223 ± 0.036
2.312GlnArg: 2.312 ± 0.029
1.189GlnSer: 1.189 ± 0.025
1.274GlnThr: 1.274 ± 0.027
2.37GlnVal: 2.37 ± 0.035
0.455GlnTrp: 0.455 ± 0.016
0.582GlnTyr: 0.582 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.729ArgAla: 9.729 ± 0.08
0.582ArgCys: 0.582 ± 0.016
4.228ArgAsp: 4.228 ± 0.05
4.846ArgGlu: 4.846 ± 0.053
2.36ArgPhe: 2.36 ± 0.029
5.734ArgGly: 5.734 ± 0.056
2.034ArgHis: 2.034 ± 0.033
3.464ArgIle: 3.464 ± 0.042
1.722ArgLys: 1.722 ± 0.031
8.703ArgLeu: 8.703 ± 0.085
1.659ArgMet: 1.659 ± 0.027
1.451ArgAsn: 1.451 ± 0.027
4.924ArgPro: 4.924 ± 0.06
2.267ArgGln: 2.267 ± 0.035
7.336ArgArg: 7.336 ± 0.083
4.2ArgSer: 4.2 ± 0.049
5.936ArgThr: 5.936 ± 0.071
5.616ArgVal: 5.616 ± 0.051
1.342ArgTrp: 1.342 ± 0.029
1.786ArgTyr: 1.786 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.694SerAla: 6.694 ± 0.064
0.448SerCys: 0.448 ± 0.013
2.66SerAsp: 2.66 ± 0.037
2.441SerGlu: 2.441 ± 0.037
1.524SerPhe: 1.524 ± 0.033
6.068SerGly: 6.068 ± 0.066
1.032SerHis: 1.032 ± 0.024
1.491SerIle: 1.491 ± 0.031
1.039SerLys: 1.039 ± 0.024
4.892SerLeu: 4.892 ± 0.053
1.094SerMet: 1.094 ± 0.024
0.837SerAsn: 0.837 ± 0.022
3.191SerPro: 3.191 ± 0.042
1.239SerGln: 1.239 ± 0.029
3.614SerArg: 3.614 ± 0.041
2.84SerSer: 2.84 ± 0.048
3.149SerThr: 3.149 ± 0.044
4.239SerVal: 4.239 ± 0.047
0.929SerTrp: 0.929 ± 0.021
1.299SerTyr: 1.299 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
8.996ThrAla: 8.996 ± 0.069
0.421ThrCys: 0.421 ± 0.017
3.651ThrAsp: 3.651 ± 0.041
3.568ThrGlu: 3.568 ± 0.043
1.675ThrPhe: 1.675 ± 0.027
7.129ThrGly: 7.129 ± 0.058
1.253ThrHis: 1.253 ± 0.024
1.726ThrIle: 1.726 ± 0.029
1.182ThrLys: 1.182 ± 0.027
5.812ThrLeu: 5.812 ± 0.056
0.917ThrMet: 0.917 ± 0.02
0.991ThrAsn: 0.991 ± 0.02
4.168ThrPro: 4.168 ± 0.055
1.274ThrGln: 1.274 ± 0.026
3.844ThrArg: 3.844 ± 0.043
3.182ThrSer: 3.182 ± 0.039
3.976ThrThr: 3.976 ± 0.064
6.361ThrVal: 6.361 ± 0.054
0.886ThrTrp: 0.886 ± 0.022
1.419ThrTyr: 1.419 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
10.391ValAla: 10.391 ± 0.077
0.751ValCys: 0.751 ± 0.02
4.947ValAsp: 4.947 ± 0.047
4.781ValGlu: 4.781 ± 0.056
2.354ValPhe: 2.354 ± 0.031
6.381ValGly: 6.381 ± 0.058
2.007ValHis: 2.007 ± 0.028
2.922ValIle: 2.922 ± 0.038
1.759ValLys: 1.759 ± 0.039
9.437ValLeu: 9.437 ± 0.075
1.396ValMet: 1.396 ± 0.027
1.642ValAsn: 1.642 ± 0.031
5.146ValPro: 5.146 ± 0.05
2.048ValGln: 2.048 ± 0.029
7.229ValArg: 7.229 ± 0.066
4.301ValSer: 4.301 ± 0.048
5.604ValThr: 5.604 ± 0.053
7.716ValVal: 7.716 ± 0.077
1.074ValTrp: 1.074 ± 0.022
1.616ValTyr: 1.616 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.629TrpAla: 1.629 ± 0.028
0.152TrpCys: 0.152 ± 0.008
0.778TrpAsp: 0.778 ± 0.019
0.76TrpGlu: 0.76 ± 0.02
0.487TrpPhe: 0.487 ± 0.013
1.012TrpGly: 1.012 ± 0.023
0.35TrpHis: 0.35 ± 0.015
0.577TrpIle: 0.577 ± 0.014
0.389TrpLys: 0.389 ± 0.014
1.717TrpLeu: 1.717 ± 0.031
0.292TrpMet: 0.292 ± 0.011
0.409TrpAsn: 0.409 ± 0.014
0.772TrpPro: 0.772 ± 0.021
0.587TrpGln: 0.587 ± 0.017
1.296TrpArg: 1.296 ± 0.028
0.941TrpSer: 0.941 ± 0.021
1.012TrpThr: 1.012 ± 0.024
0.931TrpVal: 0.931 ± 0.021
0.32TrpTrp: 0.32 ± 0.013
0.336TrpTyr: 0.336 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.833TyrAla: 2.833 ± 0.036
0.171TyrCys: 0.171 ± 0.01
1.452TyrAsp: 1.452 ± 0.031
1.284TyrGlu: 1.284 ± 0.025
0.636TyrPhe: 0.636 ± 0.018
2.367TyrGly: 2.367 ± 0.038
0.379TyrHis: 0.379 ± 0.014
0.504TyrIle: 0.504 ± 0.017
0.464TyrLys: 0.464 ± 0.014
2.116TyrLeu: 2.116 ± 0.031
0.252TyrMet: 0.252 ± 0.012
0.392TyrAsn: 0.392 ± 0.014
1.049TyrPro: 1.049 ± 0.024
0.589TyrGln: 0.589 ± 0.018
1.832TyrArg: 1.832 ± 0.027
0.899TyrSer: 0.899 ± 0.022
1.298TyrThr: 1.298 ± 0.026
1.678TyrVal: 1.678 ± 0.027
0.345TyrTrp: 0.345 ± 0.012
0.435TyrTyr: 0.435 ± 0.013
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7341 proteins (2193684 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski