Anthurium mosaic-associated virus
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9U544|D9U544_9VIRU Coat protein OS=Anthurium mosaic-associated virus OX=664255 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 5.08 SEE4 pKa = 4.65 IKK6 pKa = 10.33 NDD8 pKa = 3.39 SGRR11 pKa = 11.84 FSADD15 pKa = 2.93 KK16 pKa = 10.37 IVSRR20 pKa = 11.84 KK21 pKa = 9.06 LRR23 pKa = 11.84 AYY25 pKa = 10.44 LGSTKK30 pKa = 9.68 CTLEE34 pKa = 3.37 YY35 pKa = 10.3 RR36 pKa = 11.84 EE37 pKa = 5.12 RR38 pKa = 11.84 IFSSLGRR45 pKa = 11.84 LNDD48 pKa = 3.62 KK49 pKa = 10.76 VIEE52 pKa = 4.1 GLYY55 pKa = 10.62 AIVIPSGGGKK65 pKa = 6.03 TTMARR70 pKa = 11.84 EE71 pKa = 3.91 FGFIDD76 pKa = 3.38 VDD78 pKa = 3.86 EE79 pKa = 5.24 VIGNQAVMEE88 pKa = 4.24 KK89 pKa = 10.44 LVLEE93 pKa = 4.06 RR94 pKa = 11.84 RR95 pKa = 11.84 EE96 pKa = 4.07 LIRR99 pKa = 11.84 WGKK102 pKa = 9.93 DD103 pKa = 2.18 RR104 pKa = 11.84 WEE106 pKa = 4.02 EE107 pKa = 4.19 HH108 pKa = 5.62 NKK110 pKa = 8.51 VWYY113 pKa = 9.26 NQVHH117 pKa = 5.63 SVLAKK122 pKa = 10.53 YY123 pKa = 10.18 DD124 pKa = 3.61 FQNNPGIIMVHH135 pKa = 4.74 TEE137 pKa = 3.48 EE138 pKa = 4.04 MAYY141 pKa = 10.15 EE142 pKa = 4.77 LGAQPLIALTPKK154 pKa = 10.68 DD155 pKa = 3.52 SLYY158 pKa = 9.31 KK159 pKa = 10.49 QRR161 pKa = 11.84 MKK163 pKa = 10.9 GRR165 pKa = 11.84 NEE167 pKa = 4.15 LEE169 pKa = 3.77 RR170 pKa = 11.84 MLAVDD175 pKa = 3.07 NLEE178 pKa = 4.18 IVRR181 pKa = 11.84 HH182 pKa = 5.06 RR183 pKa = 11.84 TQSVDD188 pKa = 2.99 IIGYY192 pKa = 8.71 GSWKK196 pKa = 9.81 EE197 pKa = 3.91 LKK199 pKa = 10.34 QLIAGVAWEE208 pKa = 4.5 EE209 pKa = 4.28 VEE211 pKa = 4.04 MAAPFRR217 pKa = 11.84 DD218 pKa = 4.26 YY219 pKa = 11.11 NRR221 pKa = 11.84 KK222 pKa = 8.11 PAHH225 pKa = 6.21 GWPIGYY231 pKa = 10.25 GEE233 pKa = 5.8 DD234 pKa = 3.25 VALWIMQGKK243 pKa = 7.53 TDD245 pKa = 4.11 DD246 pKa = 4.01 VEE248 pKa = 4.32 DD249 pKa = 3.59 MRR251 pKa = 11.84 IVEE254 pKa = 4.12 RR255 pKa = 11.84 LHH257 pKa = 7.89 RR258 pKa = 11.84 EE259 pKa = 3.47 GSVPDD264 pKa = 3.38 ICLSYY269 pKa = 10.83 YY270 pKa = 9.18 SEE272 pKa = 4.75 KK273 pKa = 10.39 IYY275 pKa = 10.47 GIKK278 pKa = 10.23 KK279 pKa = 9.72 IEE281 pKa = 4.34 TKK283 pKa = 11.07 GEE285 pKa = 4.12 TLCRR289 pKa = 11.84 EE290 pKa = 4.49 WISICGEE297 pKa = 3.57 ITNCGKK303 pKa = 9.62 MDD305 pKa = 3.84 TVTEE309 pKa = 4.18 MEE311 pKa = 5.02 VIANDD316 pKa = 3.61 LNLVYY321 pKa = 9.93 PYY323 pKa = 9.87 EE324 pKa = 4.72 SKK326 pKa = 10.23 MAMNKK331 pKa = 8.36 RR332 pKa = 11.84 TIGLRR337 pKa = 11.84 RR338 pKa = 11.84 LLEE341 pKa = 4.08 NMEE344 pKa = 4.18 AMRR347 pKa = 11.84 NDD349 pKa = 2.84 IMRR352 pKa = 11.84 TVLLNRR358 pKa = 11.84 RR359 pKa = 11.84 GSNQNLVVGILIWVGGVLSNMRR381 pKa = 11.84 VEE383 pKa = 4.38 IQDD386 pKa = 3.32 SVLRR390 pKa = 11.84 SEE392 pKa = 4.5 ILLVPDD398 pKa = 3.89 EE399 pKa = 5.08 DD400 pKa = 4.17 WIKK403 pKa = 10.25 MGKK406 pKa = 9.14 KK407 pKa = 8.2 VHH409 pKa = 6.72 DD410 pKa = 3.63 MVRR413 pKa = 11.84 RR414 pKa = 11.84 TGTFFGTTINVQEE427 pKa = 4.35 AQKK430 pKa = 11.21 LMYY433 pKa = 10.91 LHH435 pKa = 6.34 MLYY438 pKa = 10.79 GRR440 pKa = 11.84 FVYY443 pKa = 10.28 QVDD446 pKa = 3.77 EE447 pKa = 4.1 EE448 pKa = 4.58 AEE450 pKa = 3.54 IDD452 pKa = 3.45 KK453 pKa = 10.77 RR454 pKa = 11.84 KK455 pKa = 10.31 RR456 pKa = 11.84 EE457 pKa = 4.05 LNDD460 pKa = 2.87 TKK462 pKa = 10.34 MAYY465 pKa = 10.38 LDD467 pKa = 3.89 GEE469 pKa = 4.26 WRR471 pKa = 11.84 VDD473 pKa = 3.49 EE474 pKa = 4.24 FDD476 pKa = 3.65 KK477 pKa = 11.75 LEE479 pKa = 4.3 LEE481 pKa = 5.06 GIRR484 pKa = 11.84 DD485 pKa = 3.98 AYY487 pKa = 10.77 RR488 pKa = 11.84 RR489 pKa = 11.84 LCDD492 pKa = 3.58 SITPQRR498 pKa = 11.84 VMDD501 pKa = 5.42 FSEE504 pKa = 4.31 FWKK507 pKa = 10.44 RR508 pKa = 11.84 RR509 pKa = 11.84 RR510 pKa = 11.84 SWAAKK515 pKa = 9.0 GSTVVYY521 pKa = 9.77 EE522 pKa = 4.3 GEE524 pKa = 4.11 KK525 pKa = 10.44 KK526 pKa = 10.83 YY527 pKa = 10.31 MLEE530 pKa = 3.88 IVDD533 pKa = 3.65 EE534 pKa = 4.78 VIRR537 pKa = 11.84 QLEE540 pKa = 3.94 LRR542 pKa = 11.84 HH543 pKa = 5.71 NKK545 pKa = 9.28 KK546 pKa = 10.67 SLMEE550 pKa = 5.48 DD551 pKa = 3.13 EE552 pKa = 5.54 AEE554 pKa = 4.12 CCKK557 pKa = 10.29 IISGIIRR564 pKa = 11.84 DD565 pKa = 4.15 LGRR568 pKa = 11.84 NDD570 pKa = 3.0 TKK572 pKa = 10.41 MVPKK576 pKa = 10.38 FEE578 pKa = 4.46 SAAKK582 pKa = 10.01 RR583 pKa = 11.84 ALLPGNLYY591 pKa = 10.26 HH592 pKa = 6.49 YY593 pKa = 10.89 VVFSYY598 pKa = 11.37 VLLIFEE604 pKa = 4.66 NCASIGDD611 pKa = 3.76 VRR613 pKa = 11.84 LGNDD617 pKa = 3.2 RR618 pKa = 11.84 DD619 pKa = 3.73 NSFGAFDD626 pKa = 4.82 SKK628 pKa = 11.58 LEE630 pKa = 4.19 TNLTRR635 pKa = 11.84 FVYY638 pKa = 10.64 DD639 pKa = 3.72 FADD642 pKa = 4.83 FNAQHH647 pKa = 6.34 SRR649 pKa = 11.84 RR650 pKa = 11.84 SMARR654 pKa = 11.84 VMSVLSEE661 pKa = 4.06 TVDD664 pKa = 3.56 PNEE667 pKa = 4.05 TLGFCLKK674 pKa = 9.93 WMSLSFNKK682 pKa = 9.79 MEE684 pKa = 4.45 VIKK687 pKa = 10.84 EE688 pKa = 4.0 GGGVEE693 pKa = 4.11 KK694 pKa = 10.56 LKK696 pKa = 11.06 SGLYY700 pKa = 9.77 SGWRR704 pKa = 11.84 GTTWINSVLNHH715 pKa = 6.59 AYY717 pKa = 9.09 MYY719 pKa = 9.67 VARR722 pKa = 11.84 VCFNRR727 pKa = 11.84 LYY729 pKa = 10.52 GYY731 pKa = 10.33 EE732 pKa = 4.07 PFVEE736 pKa = 4.22 YY737 pKa = 10.46 EE738 pKa = 4.15 GAGDD742 pKa = 4.31 DD743 pKa = 3.49 VDD745 pKa = 5.49 GVVKK749 pKa = 10.64 DD750 pKa = 3.36 ISTAGKK756 pKa = 9.65 LYY758 pKa = 10.54 RR759 pKa = 11.84 ITVEE763 pKa = 4.49 MGLEE767 pKa = 4.2 SNVQKK772 pKa = 10.9 QLFGKK777 pKa = 9.72 RR778 pKa = 11.84 AEE780 pKa = 4.17 FLRR783 pKa = 11.84 VSYY786 pKa = 10.57 EE787 pKa = 3.58 SGYY790 pKa = 10.94 AGSSICRR797 pKa = 11.84 VLGNFISGNWEE808 pKa = 3.95 GEE810 pKa = 4.2 GGSVSDD816 pKa = 4.56 RR817 pKa = 11.84 LTSAIDD823 pKa = 3.72 NILTLGRR830 pKa = 11.84 RR831 pKa = 11.84 GLDD834 pKa = 2.95 EE835 pKa = 5.52 SMVKK839 pKa = 10.03 VLYY842 pKa = 10.26 RR843 pKa = 11.84 CVLMHH848 pKa = 6.66 VGRR851 pKa = 11.84 IFDD854 pKa = 3.69 GDD856 pKa = 3.31 EE857 pKa = 4.15 WIGLSPCILHH867 pKa = 7.09 GRR869 pKa = 11.84 MEE871 pKa = 5.14 DD872 pKa = 3.34 NGFGIPDD879 pKa = 3.35 EE880 pKa = 5.23 DD881 pKa = 3.96 GCVWEE886 pKa = 4.97 LEE888 pKa = 4.49 KK889 pKa = 10.84 KK890 pKa = 10.72 APAPDD895 pKa = 3.03 GWLGYY900 pKa = 9.99 IKK902 pKa = 10.66 LPAKK906 pKa = 8.88 TASRR910 pKa = 11.84 DD911 pKa = 3.33 WVDD914 pKa = 4.26 VIDD917 pKa = 4.19 EE918 pKa = 4.55 EE919 pKa = 5.07 IRR921 pKa = 11.84 DD922 pKa = 3.76 RR923 pKa = 11.84 GLKK926 pKa = 10.35 VGDD929 pKa = 3.43 VDD931 pKa = 5.13 RR932 pKa = 11.84 LSEE935 pKa = 4.0 LLARR939 pKa = 11.84 DD940 pKa = 3.66 SYY942 pKa = 11.25 DD943 pKa = 3.32 VKK945 pKa = 11.11 SVADD949 pKa = 3.89 RR950 pKa = 11.84 FGTEE954 pKa = 3.69 ISPEE958 pKa = 3.36 IWKK961 pKa = 10.29 KK962 pKa = 9.44 YY963 pKa = 4.74 WTYY966 pKa = 10.16 KK967 pKa = 10.09 CKK969 pKa = 10.45 VVKK972 pKa = 10.05 KK973 pKa = 9.65 YY974 pKa = 10.64 KK975 pKa = 10.21 IEE977 pKa = 3.93 TDD979 pKa = 3.75 VIDD982 pKa = 4.39 EE983 pKa = 4.3 GMLLDD988 pKa = 3.84 FLEE991 pKa = 4.31 WLGGEE996 pKa = 4.14 EE997 pKa = 4.94 TPTLDD1002 pKa = 3.92 QLSKK1006 pKa = 11.55 LEE1008 pKa = 3.81 ILGPYY1013 pKa = 9.64 IEE1015 pKa = 5.26 TIVSGDD1021 pKa = 3.68 DD1022 pKa = 3.4 KK1023 pKa = 11.2 PVTEE1027 pKa = 4.31 EE1028 pKa = 3.3 EE1029 pKa = 4.97 WYY1031 pKa = 11.06 GEE1033 pKa = 4.51 LYY1035 pKa = 10.4 DD1036 pKa = 4.5 APRR1039 pKa = 11.84 RR1040 pKa = 11.84 LEE1042 pKa = 3.87 NMKK1045 pKa = 9.79 LQRR1048 pKa = 11.84 WKK1050 pKa = 11.08 VVLCPPLVQSKK1061 pKa = 9.48 IARR1064 pKa = 11.84 WCKK1067 pKa = 10.35 EE1068 pKa = 3.34 MVANDD1073 pKa = 3.69 MVTQFTGMRR1082 pKa = 11.84 IYY1084 pKa = 10.66 QIVSNTYY1091 pKa = 8.33 GSIFGFEE1098 pKa = 3.85 II1099 pKa = 4.17
Molecular weight: 126.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.305
IPC2_protein 5.397
IPC_protein 5.372
Toseland 5.359
ProMoST 5.525
Dawson 5.385
Bjellqvist 5.499
Wikipedia 5.258
Rodwell 5.321
Grimsley 5.308
Solomon 5.385
Lehninger 5.347
Nozaki 5.525
DTASelect 5.664
Thurlkill 5.397
EMBOSS 5.347
Sillero 5.601
Patrickios 4.215
IPC_peptide 5.397
IPC2_peptide 5.601
IPC2.peptide.svr19 5.609
Protein with the highest isoelectric point:
>tr|D9U547|D9U547_9VIRU p26 OS=Anthurium mosaic-associated virus OX=664255 PE=4 SV=1
MM1 pKa = 6.31 TTPYY5 pKa = 10.36 PRR7 pKa = 11.84 FAINRR12 pKa = 11.84 AAGSISNQSFFSCSLLKK29 pKa = 10.95 YY30 pKa = 8.67 STNSLPVRR38 pKa = 11.84 LCPCSLRR45 pKa = 11.84 PSHH48 pKa = 6.36 NAWATHH54 pKa = 5.73 NPSPTTCFTFSFVPSAVTSLMNVSIPLNPKK84 pKa = 9.87 LSAIFLMKK92 pKa = 10.66 LIFGGFLAFRR102 pKa = 11.84 PPLFNLSVASMSSSSLLSFPFSGFTPASAFVFSFLLSPLFCLSSFSILSSRR153 pKa = 11.84 SLCALSFCSLLSFSIPRR170 pKa = 11.84 YY171 pKa = 8.32 VLKK174 pKa = 10.68 KK175 pKa = 10.29 SNLSFSIVSLPTLGSYY191 pKa = 10.93 SSFQFVSRR199 pKa = 11.84 YY200 pKa = 6.67 TSSYY204 pKa = 8.75 TPYY207 pKa = 9.61 PPSILAIVLWLTISSPIFCIALYY230 pKa = 8.18 PQNIIISSLSPP241 pKa = 3.44
Molecular weight: 26.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.271
IPC2_protein 9.428
IPC_protein 9.589
Toseland 9.516
ProMoST 9.545
Dawson 9.911
Bjellqvist 9.809
Wikipedia 10.145
Rodwell 10.014
Grimsley 10.014
Solomon 9.955
Lehninger 9.897
Nozaki 9.867
DTASelect 9.721
Thurlkill 9.736
EMBOSS 9.999
Sillero 9.911
Patrickios 0.909
IPC_peptide 9.94
IPC2_peptide 9.238
IPC2.peptide.svr19 8.392
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3544
241
1099
708.8
80.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.91 ± 0.421
2.088 ± 0.267
4.853 ± 0.898
8.465 ± 1.922
4.007 ± 1.441
7.562 ± 1.287
1.467 ± 0.224
6.405 ± 0.279
7.336 ± 1.319
9.255 ± 1.277
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.386 ± 0.375
4.374 ± 0.491
3.612 ± 1.218
1.862 ± 0.226
6.01 ± 0.922
8.775 ± 2.653
4.402 ± 0.221
6.123 ± 0.714
2.088 ± 0.252
3.019 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here