Amino acid dipepetide frequency for Pseudomonas phage KP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.013AlaAla: 13.013 ± 2.604
0.603AlaCys: 0.603 ± 0.217
4.395AlaAsp: 4.395 ± 0.597
5.946AlaGlu: 5.946 ± 0.78
2.585AlaPhe: 2.585 ± 0.391
6.808AlaGly: 6.808 ± 1.036
1.465AlaHis: 1.465 ± 0.395
5.515AlaIle: 5.515 ± 0.796
6.377AlaLys: 6.377 ± 0.954
8.962AlaLeu: 8.962 ± 0.978
1.896AlaMet: 1.896 ± 0.481
4.998AlaAsn: 4.998 ± 0.848
4.481AlaPro: 4.481 ± 0.754
4.998AlaGln: 4.998 ± 1.141
4.826AlaArg: 4.826 ± 0.576
8.359AlaSer: 8.359 ± 0.973
7.067AlaThr: 7.067 ± 1.193
8.273AlaVal: 8.273 ± 1.051
1.206AlaTrp: 1.206 ± 0.427
3.447AlaTyr: 3.447 ± 0.48
0.0AlaXaa: 0.0 ± 0.0
Cys
0.776CysAla: 0.776 ± 0.304
0.431CysCys: 0.431 ± 0.238
0.948CysAsp: 0.948 ± 0.275
0.862CysGlu: 0.862 ± 0.242
0.431CysPhe: 0.431 ± 0.217
0.948CysGly: 0.948 ± 0.369
0.517CysHis: 0.517 ± 0.235
0.431CysIle: 0.431 ± 0.177
0.345CysLys: 0.345 ± 0.25
0.862CysLeu: 0.862 ± 0.265
0.086CysMet: 0.086 ± 0.071
0.517CysAsn: 0.517 ± 0.189
0.862CysPro: 0.862 ± 0.303
0.0CysGln: 0.0 ± 0.0
0.603CysArg: 0.603 ± 0.189
0.431CysSer: 0.431 ± 0.184
0.431CysThr: 0.431 ± 0.178
0.431CysVal: 0.431 ± 0.18
0.0CysTrp: 0.0 ± 0.0
0.603CysTyr: 0.603 ± 0.249
0.0CysXaa: 0.0 ± 0.0
Asp
5.515AspAla: 5.515 ± 0.839
0.517AspCys: 0.517 ± 0.228
2.068AspAsp: 2.068 ± 0.462
4.223AspGlu: 4.223 ± 0.564
2.413AspPhe: 2.413 ± 0.424
4.912AspGly: 4.912 ± 0.802
0.603AspHis: 0.603 ± 0.28
2.413AspIle: 2.413 ± 0.35
3.102AspLys: 3.102 ± 0.48
4.654AspLeu: 4.654 ± 0.455
1.206AspMet: 1.206 ± 0.239
2.499AspAsn: 2.499 ± 0.356
2.93AspPro: 2.93 ± 0.39
1.293AspGln: 1.293 ± 0.426
2.585AspArg: 2.585 ± 0.493
2.758AspSer: 2.758 ± 0.623
2.499AspThr: 2.499 ± 0.492
3.102AspVal: 3.102 ± 0.489
0.517AspTrp: 0.517 ± 0.178
2.241AspTyr: 2.241 ± 0.468
0.0AspXaa: 0.0 ± 0.0
Glu
5.688GluAla: 5.688 ± 0.897
0.345GluCys: 0.345 ± 0.142
2.93GluAsp: 2.93 ± 0.641
4.481GluGlu: 4.481 ± 0.722
2.068GluPhe: 2.068 ± 0.441
3.447GluGly: 3.447 ± 0.652
1.12GluHis: 1.12 ± 0.381
3.878GluIle: 3.878 ± 0.689
3.447GluLys: 3.447 ± 0.612
5.774GluLeu: 5.774 ± 0.641
1.896GluMet: 1.896 ± 0.344
2.413GluAsn: 2.413 ± 0.34
1.637GluPro: 1.637 ± 0.45
2.671GluGln: 2.671 ± 0.516
3.619GluArg: 3.619 ± 0.483
2.93GluSer: 2.93 ± 0.435
3.878GluThr: 3.878 ± 0.603
4.395GluVal: 4.395 ± 0.624
1.465GluTrp: 1.465 ± 0.444
2.327GluTyr: 2.327 ± 0.349
0.0GluXaa: 0.0 ± 0.0
Phe
3.533PheAla: 3.533 ± 0.596
0.603PheCys: 0.603 ± 0.22
3.619PheAsp: 3.619 ± 0.513
2.585PheGlu: 2.585 ± 0.44
1.637PhePhe: 1.637 ± 0.414
2.93PheGly: 2.93 ± 0.494
0.517PheHis: 0.517 ± 0.235
2.241PheIle: 2.241 ± 0.339
2.068PheLys: 2.068 ± 0.394
1.379PheLeu: 1.379 ± 0.325
1.12PheMet: 1.12 ± 0.306
2.154PheAsn: 2.154 ± 0.406
1.551PhePro: 1.551 ± 0.329
1.12PheGln: 1.12 ± 0.319
2.068PheArg: 2.068 ± 0.342
2.241PheSer: 2.241 ± 0.506
2.154PheThr: 2.154 ± 0.577
3.102PheVal: 3.102 ± 0.521
0.517PheTrp: 0.517 ± 0.182
1.379PheTyr: 1.379 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
6.98GlyAla: 6.98 ± 1.045
1.206GlyCys: 1.206 ± 0.375
3.706GlyAsp: 3.706 ± 0.516
4.309GlyGlu: 4.309 ± 0.592
3.533GlyPhe: 3.533 ± 0.468
5.171GlyGly: 5.171 ± 0.791
0.948GlyHis: 0.948 ± 0.367
3.361GlyIle: 3.361 ± 0.585
3.792GlyLys: 3.792 ± 0.476
7.411GlyLeu: 7.411 ± 0.941
1.724GlyMet: 1.724 ± 0.354
3.102GlyAsn: 3.102 ± 0.452
2.327GlyPro: 2.327 ± 0.4
3.102GlyGln: 3.102 ± 0.613
3.533GlyArg: 3.533 ± 0.495
4.481GlySer: 4.481 ± 0.758
5.257GlyThr: 5.257 ± 0.593
5.946GlyVal: 5.946 ± 0.743
1.12GlyTrp: 1.12 ± 0.399
2.068GlyTyr: 2.068 ± 0.306
0.0GlyXaa: 0.0 ± 0.0
His
1.551HisAla: 1.551 ± 0.42
0.517HisCys: 0.517 ± 0.192
0.603HisAsp: 0.603 ± 0.214
0.776HisGlu: 0.776 ± 0.269
0.517HisPhe: 0.517 ± 0.201
0.689HisGly: 0.689 ± 0.248
0.172HisHis: 0.172 ± 0.106
0.948HisIle: 0.948 ± 0.313
0.603HisLys: 0.603 ± 0.218
1.379HisLeu: 1.379 ± 0.351
0.345HisMet: 0.345 ± 0.161
0.689HisAsn: 0.689 ± 0.275
0.603HisPro: 0.603 ± 0.246
0.345HisGln: 0.345 ± 0.175
1.034HisArg: 1.034 ± 0.401
1.12HisSer: 1.12 ± 0.293
0.689HisThr: 0.689 ± 0.278
1.379HisVal: 1.379 ± 0.331
0.172HisTrp: 0.172 ± 0.125
0.345HisTyr: 0.345 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
6.98IleAla: 6.98 ± 0.722
0.517IleCys: 0.517 ± 0.199
4.05IleAsp: 4.05 ± 0.504
4.74IleGlu: 4.74 ± 0.763
1.551IlePhe: 1.551 ± 0.358
4.137IleGly: 4.137 ± 0.681
1.206IleHis: 1.206 ± 0.429
2.499IleIle: 2.499 ± 0.453
3.275IleLys: 3.275 ± 0.457
2.93IleLeu: 2.93 ± 0.46
0.948IleMet: 0.948 ± 0.384
3.275IleAsn: 3.275 ± 0.361
3.189IlePro: 3.189 ± 0.534
1.724IleGln: 1.724 ± 0.342
1.724IleArg: 1.724 ± 0.278
3.102IleSer: 3.102 ± 0.482
3.878IleThr: 3.878 ± 0.467
3.102IleVal: 3.102 ± 0.529
0.259IleTrp: 0.259 ± 0.152
2.068IleTyr: 2.068 ± 0.416
0.0IleXaa: 0.0 ± 0.0
Lys
6.722LysAla: 6.722 ± 0.783
0.517LysCys: 0.517 ± 0.223
2.413LysAsp: 2.413 ± 0.517
3.447LysGlu: 3.447 ± 0.628
2.499LysPhe: 2.499 ± 0.426
3.447LysGly: 3.447 ± 0.739
0.862LysHis: 0.862 ± 0.282
2.585LysIle: 2.585 ± 0.476
4.309LysLys: 4.309 ± 0.817
6.032LysLeu: 6.032 ± 0.684
1.379LysMet: 1.379 ± 0.307
3.016LysAsn: 3.016 ± 0.564
2.241LysPro: 2.241 ± 0.489
2.241LysGln: 2.241 ± 0.455
2.585LysArg: 2.585 ± 0.427
3.964LysSer: 3.964 ± 0.609
3.619LysThr: 3.619 ± 0.571
3.964LysVal: 3.964 ± 0.779
0.689LysTrp: 0.689 ± 0.25
1.637LysTyr: 1.637 ± 0.281
0.0LysXaa: 0.0 ± 0.0
Leu
8.273LeuAla: 8.273 ± 0.954
0.689LeuCys: 0.689 ± 0.237
4.395LeuAsp: 4.395 ± 0.563
4.998LeuGlu: 4.998 ± 0.534
3.447LeuPhe: 3.447 ± 0.597
4.74LeuGly: 4.74 ± 0.58
1.637LeuHis: 1.637 ± 0.493
5.343LeuIle: 5.343 ± 0.623
5.602LeuLys: 5.602 ± 0.675
5.774LeuLeu: 5.774 ± 0.779
1.896LeuMet: 1.896 ± 0.493
5.257LeuAsn: 5.257 ± 0.592
4.137LeuPro: 4.137 ± 0.594
3.102LeuGln: 3.102 ± 0.471
3.619LeuArg: 3.619 ± 0.456
5.774LeuSer: 5.774 ± 0.703
4.654LeuThr: 4.654 ± 0.829
3.792LeuVal: 3.792 ± 0.552
1.206LeuTrp: 1.206 ± 0.276
2.93LeuTyr: 2.93 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
3.016MetAla: 3.016 ± 0.616
0.086MetCys: 0.086 ± 0.081
0.603MetAsp: 0.603 ± 0.186
0.603MetGlu: 0.603 ± 0.24
0.776MetPhe: 0.776 ± 0.197
1.724MetGly: 1.724 ± 0.336
0.431MetHis: 0.431 ± 0.245
1.379MetIle: 1.379 ± 0.282
1.896MetLys: 1.896 ± 0.42
2.068MetLeu: 2.068 ± 0.541
0.776MetMet: 0.776 ± 0.194
0.862MetAsn: 0.862 ± 0.301
2.154MetPro: 2.154 ± 0.499
0.689MetGln: 0.689 ± 0.263
1.379MetArg: 1.379 ± 0.324
1.982MetSer: 1.982 ± 0.454
1.81MetThr: 1.81 ± 0.35
0.603MetVal: 0.603 ± 0.184
0.259MetTrp: 0.259 ± 0.14
0.517MetTyr: 0.517 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
4.826AsnAla: 4.826 ± 0.726
0.862AsnCys: 0.862 ± 0.289
2.758AsnAsp: 2.758 ± 0.441
3.102AsnGlu: 3.102 ± 0.44
1.12AsnPhe: 1.12 ± 0.281
4.912AsnGly: 4.912 ± 0.665
0.603AsnHis: 0.603 ± 0.197
3.361AsnIle: 3.361 ± 0.649
3.361AsnLys: 3.361 ± 0.61
3.102AsnLeu: 3.102 ± 0.474
0.862AsnMet: 0.862 ± 0.251
2.154AsnAsn: 2.154 ± 0.544
2.671AsnPro: 2.671 ± 0.414
1.724AsnGln: 1.724 ± 0.405
2.844AsnArg: 2.844 ± 0.424
3.102AsnSer: 3.102 ± 0.504
3.189AsnThr: 3.189 ± 0.519
4.74AsnVal: 4.74 ± 0.475
0.517AsnTrp: 0.517 ± 0.209
1.206AsnTyr: 1.206 ± 0.333
0.0AsnXaa: 0.0 ± 0.0
Pro
4.567ProAla: 4.567 ± 0.688
0.517ProCys: 0.517 ± 0.197
2.499ProAsp: 2.499 ± 0.571
2.499ProGlu: 2.499 ± 0.502
1.724ProPhe: 1.724 ± 0.343
4.309ProGly: 4.309 ± 0.772
0.862ProHis: 0.862 ± 0.268
3.189ProIle: 3.189 ± 0.453
1.81ProLys: 1.81 ± 0.404
3.533ProLeu: 3.533 ± 0.447
1.206ProMet: 1.206 ± 0.365
2.585ProAsn: 2.585 ± 0.474
2.93ProPro: 2.93 ± 0.54
1.724ProGln: 1.724 ± 0.41
1.551ProArg: 1.551 ± 0.367
3.189ProSer: 3.189 ± 0.414
3.275ProThr: 3.275 ± 0.582
3.361ProVal: 3.361 ± 0.463
0.689ProTrp: 0.689 ± 0.208
1.896ProTyr: 1.896 ± 0.381
0.0ProXaa: 0.0 ± 0.0
Gln
5.429GlnAla: 5.429 ± 1.449
0.259GlnCys: 0.259 ± 0.133
1.81GlnAsp: 1.81 ± 0.433
1.206GlnGlu: 1.206 ± 0.381
1.206GlnPhe: 1.206 ± 0.381
2.585GlnGly: 2.585 ± 0.536
0.345GlnHis: 0.345 ± 0.178
2.413GlnIle: 2.413 ± 0.47
1.896GlnLys: 1.896 ± 0.478
4.567GlnLeu: 4.567 ± 0.811
1.12GlnMet: 1.12 ± 0.38
2.068GlnAsn: 2.068 ± 0.722
1.465GlnPro: 1.465 ± 0.311
4.912GlnGln: 4.912 ± 2.485
2.585GlnArg: 2.585 ± 0.521
2.413GlnSer: 2.413 ± 0.515
2.93GlnThr: 2.93 ± 0.553
1.982GlnVal: 1.982 ± 0.354
0.431GlnTrp: 0.431 ± 0.177
0.862GlnTyr: 0.862 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
3.533ArgAla: 3.533 ± 0.455
0.172ArgCys: 0.172 ± 0.13
2.93ArgAsp: 2.93 ± 0.531
2.068ArgGlu: 2.068 ± 0.537
2.327ArgPhe: 2.327 ± 0.483
2.241ArgGly: 2.241 ± 0.432
0.517ArgHis: 0.517 ± 0.19
3.964ArgIle: 3.964 ± 0.572
3.016ArgLys: 3.016 ± 0.557
5.688ArgLeu: 5.688 ± 0.497
1.465ArgMet: 1.465 ± 0.364
3.189ArgAsn: 3.189 ± 0.571
2.499ArgPro: 2.499 ± 0.59
2.327ArgGln: 2.327 ± 0.408
2.327ArgArg: 2.327 ± 0.615
2.758ArgSer: 2.758 ± 0.462
3.016ArgThr: 3.016 ± 0.429
2.499ArgVal: 2.499 ± 0.364
1.034ArgTrp: 1.034 ± 0.234
1.379ArgTyr: 1.379 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
6.722SerAla: 6.722 ± 0.782
0.431SerCys: 0.431 ± 0.222
3.964SerAsp: 3.964 ± 0.505
3.361SerGlu: 3.361 ± 0.576
2.327SerPhe: 2.327 ± 0.453
5.86SerGly: 5.86 ± 0.756
0.776SerHis: 0.776 ± 0.273
3.189SerIle: 3.189 ± 0.5
4.654SerLys: 4.654 ± 0.545
4.223SerLeu: 4.223 ± 0.631
1.379SerMet: 1.379 ± 0.289
3.016SerAsn: 3.016 ± 0.515
2.93SerPro: 2.93 ± 0.447
3.275SerGln: 3.275 ± 0.773
3.016SerArg: 3.016 ± 0.492
3.533SerSer: 3.533 ± 0.521
3.878SerThr: 3.878 ± 0.473
4.05SerVal: 4.05 ± 0.572
0.431SerTrp: 0.431 ± 0.183
2.241SerTyr: 2.241 ± 0.405
0.0SerXaa: 0.0 ± 0.0
Thr
6.722ThrAla: 6.722 ± 1.119
0.259ThrCys: 0.259 ± 0.146
2.671ThrAsp: 2.671 ± 0.366
4.481ThrGlu: 4.481 ± 0.549
3.016ThrPhe: 3.016 ± 0.592
6.205ThrGly: 6.205 ± 0.931
0.689ThrHis: 0.689 ± 0.262
3.275ThrIle: 3.275 ± 0.582
2.499ThrLys: 2.499 ± 0.534
4.395ThrLeu: 4.395 ± 0.619
1.465ThrMet: 1.465 ± 0.313
3.361ThrAsn: 3.361 ± 0.42
3.275ThrPro: 3.275 ± 0.456
2.585ThrGln: 2.585 ± 0.603
2.241ThrArg: 2.241 ± 0.421
3.275ThrSer: 3.275 ± 0.694
3.275ThrThr: 3.275 ± 0.581
4.74ThrVal: 4.74 ± 0.947
0.948ThrTrp: 0.948 ± 0.25
1.896ThrTyr: 1.896 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
7.756ValAla: 7.756 ± 0.797
0.776ValCys: 0.776 ± 0.227
3.016ValAsp: 3.016 ± 0.41
4.309ValGlu: 4.309 ± 0.635
3.275ValPhe: 3.275 ± 0.463
4.395ValGly: 4.395 ± 0.679
0.603ValHis: 0.603 ± 0.16
3.361ValIle: 3.361 ± 0.516
3.619ValLys: 3.619 ± 0.524
5.171ValLeu: 5.171 ± 0.815
1.551ValMet: 1.551 ± 0.338
3.533ValAsn: 3.533 ± 0.531
3.533ValPro: 3.533 ± 0.546
2.758ValGln: 2.758 ± 0.555
3.619ValArg: 3.619 ± 0.454
4.309ValSer: 4.309 ± 0.603
3.533ValThr: 3.533 ± 0.81
3.706ValVal: 3.706 ± 0.413
1.379ValTrp: 1.379 ± 0.315
2.068ValTyr: 2.068 ± 0.532
0.0ValXaa: 0.0 ± 0.0
Trp
0.689TrpAla: 0.689 ± 0.189
0.603TrpCys: 0.603 ± 0.351
0.603TrpAsp: 0.603 ± 0.362
0.603TrpGlu: 0.603 ± 0.218
0.776TrpPhe: 0.776 ± 0.259
0.948TrpGly: 0.948 ± 0.457
0.086TrpHis: 0.086 ± 0.076
0.345TrpIle: 0.345 ± 0.228
0.689TrpLys: 0.689 ± 0.227
1.034TrpLeu: 1.034 ± 0.233
0.431TrpMet: 0.431 ± 0.171
0.431TrpAsn: 0.431 ± 0.188
1.12TrpPro: 1.12 ± 0.331
0.689TrpGln: 0.689 ± 0.205
1.551TrpArg: 1.551 ± 0.362
0.431TrpSer: 0.431 ± 0.207
0.259TrpThr: 0.259 ± 0.124
1.206TrpVal: 1.206 ± 0.318
0.345TrpTrp: 0.345 ± 0.191
0.776TrpTyr: 0.776 ± 0.338
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.93TyrAla: 2.93 ± 0.696
0.603TyrCys: 0.603 ± 0.345
2.068TyrAsp: 2.068 ± 0.478
1.81TyrGlu: 1.81 ± 0.291
1.293TyrPhe: 1.293 ± 0.341
2.671TyrGly: 2.671 ± 0.48
0.517TyrHis: 0.517 ± 0.193
1.465TyrIle: 1.465 ± 0.372
1.81TyrLys: 1.81 ± 0.475
2.327TyrLeu: 2.327 ± 0.412
0.776TyrMet: 0.776 ± 0.373
1.982TyrAsn: 1.982 ± 0.449
1.379TyrPro: 1.379 ± 0.324
1.206TyrGln: 1.206 ± 0.366
1.637TyrArg: 1.637 ± 0.293
3.102TyrSer: 3.102 ± 0.511
1.982TyrThr: 1.982 ± 0.439
1.896TyrVal: 1.896 ± 0.389
0.431TyrTrp: 0.431 ± 0.197
0.862TyrTyr: 0.862 ± 0.273
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (11605 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski