Pontibacillus chungwhensis BH030062

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Pontibacillus; Pontibacillus chungwhensis

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2V383|A0A0A2V383_9BACI Uncharacterized protein OS=Pontibacillus chungwhensis BH030062 OX=1385513 GN=N780_10730 PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 8.85YY4 pKa = 9.04TIVDD8 pKa = 3.65KK9 pKa = 8.27EE10 pKa = 4.35TCIACGACGAAAPDD24 pKa = 3.33IYY26 pKa = 10.99DD27 pKa = 3.84YY28 pKa = 11.45DD29 pKa = 4.3DD30 pKa = 3.73EE31 pKa = 7.05GIAFVILDD39 pKa = 4.14DD40 pKa = 4.17NKK42 pKa = 9.6GTAEE46 pKa = 3.94IPEE49 pKa = 4.64DD50 pKa = 3.46LHH52 pKa = 9.17EE53 pKa = 4.93DD54 pKa = 3.49MEE56 pKa = 5.08DD57 pKa = 3.5AFEE60 pKa = 4.47GCPTDD65 pKa = 3.74SIKK68 pKa = 10.93VEE70 pKa = 4.37DD71 pKa = 4.43EE72 pKa = 4.36PFDD75 pKa = 4.46GDD77 pKa = 3.75ALKK80 pKa = 11.02FEE82 pKa = 4.68

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2UWR7|A0A0A2UWR7_9BACI 4-hydroxy-tetrahydrodipicolinate synthase OS=Pontibacillus chungwhensis BH030062 OX=1385513 GN=dapA PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.5KK14 pKa = 9.25VHH16 pKa = 5.37GFRR19 pKa = 11.84NRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.37GRR39 pKa = 11.84KK40 pKa = 6.43TLSAA44 pKa = 4.15

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3684

0

3684

1063223

29

1494

288.6

32.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.737 ± 0.039

0.616 ± 0.011

5.426 ± 0.04

7.99 ± 0.053

4.46 ± 0.031

7.041 ± 0.039

2.256 ± 0.021

7.063 ± 0.04

6.265 ± 0.038

9.539 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.886 ± 0.02

4.07 ± 0.027

3.677 ± 0.022

4.106 ± 0.032

4.124 ± 0.034

6.249 ± 0.037

5.495 ± 0.027

7.24 ± 0.031

1.098 ± 0.014

3.664 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski