Camelpox virus (strain CMS)
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 261 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8QQ77|Q8QQ77_CAMPS CMP32L OS=Camelpox virus (strain CMS) OX=203172 GN=CMP32L PE=4 SV=1
MM1 pKa = 7.92 DD2 pKa = 4.65 NSMLPMFICNNIVDD16 pKa = 4.37 YY17 pKa = 11.49 VDD19 pKa = 5.93 DD20 pKa = 5.27 IDD22 pKa = 7.0 DD23 pKa = 4.46 IDD25 pKa = 6.7 DD26 pKa = 4.29 IDD28 pKa = 6.8 DD29 pKa = 4.29 IDD31 pKa = 6.97 DD32 pKa = 4.88 IDD34 pKa = 6.71 DD35 pKa = 3.73 IDD37 pKa = 3.96 NGIVQDD43 pKa = 3.86 IVQDD47 pKa = 3.84 IEE49 pKa = 5.58 DD50 pKa = 3.87 EE51 pKa = 4.28 ASNNDD56 pKa = 3.26 DD57 pKa = 3.51 HH58 pKa = 8.36 NYY60 pKa = 10.23 VYY62 pKa = 10.27 PLPEE66 pKa = 3.83 NMVYY70 pKa = 10.53 RR71 pKa = 11.84 FDD73 pKa = 3.76 KK74 pKa = 10.0 STNILDD80 pKa = 3.9 YY81 pKa = 11.21 LSTEE85 pKa = 4.1 RR86 pKa = 11.84 DD87 pKa = 3.57 HH88 pKa = 10.24 VMMAVQYY95 pKa = 10.31 YY96 pKa = 8.63 MSKK99 pKa = 10.26 QRR101 pKa = 11.84 LDD103 pKa = 3.35 DD104 pKa = 4.85 LYY106 pKa = 11.09 RR107 pKa = 11.84 QLPTKK112 pKa = 8.67 TRR114 pKa = 11.84 SYY116 pKa = 10.88 IDD118 pKa = 4.43 IINMYY123 pKa = 9.31 CDD125 pKa = 3.99 KK126 pKa = 11.33 VNNDD130 pKa = 3.47 YY131 pKa = 11.25 NRR133 pKa = 11.84 DD134 pKa = 3.51 MNIMYY139 pKa = 10.73 DD140 pKa = 3.5 MASTEE145 pKa = 4.21 SFTVYY150 pKa = 10.63 DD151 pKa = 3.93 INNEE155 pKa = 3.96 VNTILIDD162 pKa = 3.78 NKK164 pKa = 10.86 GLGVRR169 pKa = 11.84 LATISFITEE178 pKa = 4.16 LGRR181 pKa = 11.84 RR182 pKa = 11.84 CMNPVEE188 pKa = 4.32 TIKK191 pKa = 10.52 MFTLLSHH198 pKa = 6.92 TICDD202 pKa = 3.94 DD203 pKa = 3.66 CFVDD207 pKa = 4.46 YY208 pKa = 9.46 ITDD211 pKa = 3.65 ISPPNNTISNTSTRR225 pKa = 11.84 EE226 pKa = 3.74 YY227 pKa = 10.72 LKK229 pKa = 10.99 LIGITAIMFATYY241 pKa = 7.95 KK242 pKa = 8.27 TLKK245 pKa = 10.56 YY246 pKa = 10.15 MIGG249 pKa = 3.33
Molecular weight: 28.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.91
IPC_protein 3.948
Toseland 3.719
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.923
Rodwell 3.77
Grimsley 3.617
Solomon 3.961
Lehninger 3.91
Nozaki 4.075
DTASelect 4.38
Thurlkill 3.77
EMBOSS 3.935
Sillero 4.075
Patrickios 1.049
IPC_peptide 3.948
IPC2_peptide 4.05
IPC2.peptide.svr19 3.95
Protein with the highest isoelectric point:
>tr|Q8QQ13|Q8QQ13_CAMPS CMP176.5aL OS=Camelpox virus (strain CMS) OX=203172 GN=CMP176.5aL PE=4 SV=1
MM1 pKa = 7.65 LYY3 pKa = 10.11 ISVRR7 pKa = 11.84 KK8 pKa = 9.81 KK9 pKa = 10.42 RR10 pKa = 11.84 ISNTHH15 pKa = 5.49 VDD17 pKa = 2.95 SSRR20 pKa = 11.84 YY21 pKa = 8.66 INLGRR26 pKa = 11.84 RR27 pKa = 11.84 LPRR30 pKa = 11.84 HH31 pKa = 5.03 TCALSKK37 pKa = 10.22 WKK39 pKa = 10.47 DD40 pKa = 3.52 VAVLCLQIGQNHH52 pKa = 7.34 GIWILVVSRR61 pKa = 11.84 SILQMNGCHH70 pKa = 6.39 LL71 pKa = 4.09
Molecular weight: 8.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.794
IPC_protein 10.613
Toseland 10.789
ProMoST 10.482
Dawson 10.877
Bjellqvist 10.599
Wikipedia 11.082
Rodwell 11.111
Grimsley 10.921
Solomon 11.008
Lehninger 10.979
Nozaki 10.789
DTASelect 10.584
Thurlkill 10.774
EMBOSS 11.184
Sillero 10.818
Patrickios 10.906
IPC_peptide 11.008
IPC2_peptide 9.897
IPC2.peptide.svr19 8.362
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
261
0
261
62949
53
1869
241.2
27.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.746 ± 0.115
2.13 ± 0.094
6.44 ± 0.112
5.117 ± 0.129
4.693 ± 0.129
3.937 ± 0.099
2.167 ± 0.087
9.665 ± 0.189
6.966 ± 0.117
9.003 ± 0.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.813 ± 0.086
6.901 ± 0.125
3.358 ± 0.102
2.2 ± 0.073
4.316 ± 0.11
8.181 ± 0.147
6.13 ± 0.139
6.132 ± 0.085
0.758 ± 0.041
5.347 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here