Asikkala orthohantavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Hantaviridae; Mammantavirinae; Orthohantavirus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JAI3|R4JAI3_9VIRU Envelopment polyprotein (Fragment) OS=Asikkala orthohantavirus OX=1980458 PE=3 SV=1
MM1 pKa = 7.68GDD3 pKa = 3.0IKK5 pKa = 10.84EE6 pKa = 4.5LEE8 pKa = 4.04RR9 pKa = 11.84EE10 pKa = 4.28LKK12 pKa = 10.43NVEE15 pKa = 4.07GQLEE19 pKa = 4.11IAQKK23 pKa = 10.13KK24 pKa = 9.52LEE26 pKa = 4.43RR27 pKa = 11.84ATSDD31 pKa = 3.39YY32 pKa = 10.32QTDD35 pKa = 3.56DD36 pKa = 4.52DD37 pKa = 4.38EE38 pKa = 5.0TNKK41 pKa = 9.21ATYY44 pKa = 10.07DD45 pKa = 3.35RR46 pKa = 11.84RR47 pKa = 11.84TLEE50 pKa = 4.0VSQLQAKK57 pKa = 9.33IAQLKK62 pKa = 10.24KK63 pKa = 10.58SLADD67 pKa = 3.1ATAAGRR73 pKa = 11.84QSMAAAEE80 pKa = 4.5DD81 pKa = 4.01PTGKK85 pKa = 10.54EE86 pKa = 3.59SDD88 pKa = 4.18DD89 pKa = 3.82YY90 pKa = 11.52LGQRR94 pKa = 11.84SMLRR98 pKa = 11.84YY99 pKa = 10.42GNTIDD104 pKa = 4.83VNAIDD109 pKa = 4.84LDD111 pKa = 4.17EE112 pKa = 5.39PSGQTADD119 pKa = 3.49WISIITYY126 pKa = 9.72ILTFVDD132 pKa = 4.67TILLKK137 pKa = 10.59GLYY140 pKa = 9.13MLTTRR145 pKa = 11.84GRR147 pKa = 11.84QTVKK151 pKa = 10.63DD152 pKa = 3.77NKK154 pKa = 8.95GTRR157 pKa = 11.84IRR159 pKa = 11.84LKK161 pKa = 10.71DD162 pKa = 3.34DD163 pKa = 3.19TSFVDD168 pKa = 3.53TEE170 pKa = 4.21TGRR173 pKa = 11.84KK174 pKa = 8.33PRR176 pKa = 11.84HH177 pKa = 6.09LYY179 pKa = 10.19ISMPNAQSSMRR190 pKa = 11.84ADD192 pKa = 4.39EE193 pKa = 4.25ITPGRR198 pKa = 11.84YY199 pKa = 7.28RR200 pKa = 11.84TVVCGLFPAQVRR212 pKa = 11.84QRR214 pKa = 11.84QMISPVMGVIGFPVIAKK231 pKa = 9.27NWTDD235 pKa = 3.73RR236 pKa = 11.84IEE238 pKa = 3.95QFLASDD244 pKa = 4.36CPFLKK249 pKa = 10.28QVLQVALLSPDD260 pKa = 3.2KK261 pKa = 11.22NRR263 pKa = 11.84DD264 pKa = 3.75FISDD268 pKa = 3.55RR269 pKa = 11.84QANLDD274 pKa = 3.55KK275 pKa = 10.67MITEE279 pKa = 4.69EE280 pKa = 4.1ARR282 pKa = 11.84KK283 pKa = 9.38IQDD286 pKa = 3.69AIAAKK291 pKa = 9.94SHH293 pKa = 5.49TVPTSLDD300 pKa = 3.43SPFSVWVFAGAPDD313 pKa = 4.0RR314 pKa = 11.84CPPTSLYY321 pKa = 9.2VAGMAEE327 pKa = 4.25LGAFFSILQDD337 pKa = 3.29MRR339 pKa = 11.84NTIIASKK346 pKa = 9.36TVGTAEE352 pKa = 4.09EE353 pKa = 4.16KK354 pKa = 10.68LKK356 pKa = 10.88KK357 pKa = 10.03KK358 pKa = 10.65SSFYY362 pKa = 10.41QSYY365 pKa = 10.61LRR367 pKa = 11.84RR368 pKa = 11.84TQSMGVQLDD377 pKa = 3.15QRR379 pKa = 11.84IIVLYY384 pKa = 6.32MTAWGKK390 pKa = 9.59EE391 pKa = 4.2AVDD394 pKa = 4.16HH395 pKa = 6.05FHH397 pKa = 7.34LGDD400 pKa = 4.56DD401 pKa = 3.88MDD403 pKa = 4.97PEE405 pKa = 4.6LRR407 pKa = 11.84ATAQNLIDD415 pKa = 3.87QKK417 pKa = 11.32VKK419 pKa = 10.41EE420 pKa = 4.23ISNMEE425 pKa = 4.11PMKK428 pKa = 10.89LL429 pKa = 3.47

Molecular weight: 48.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JAI5|R4JAI5_9VIRU Replicase (Fragment) OS=Asikkala orthohantavirus OX=1980458 PE=4 SV=1
SS1 pKa = 6.93IDD3 pKa = 3.92EE4 pKa = 4.1LVEE7 pKa = 4.09YY8 pKa = 9.76CKK10 pKa = 10.6KK11 pKa = 9.96WLSSPHH17 pKa = 6.25KK18 pKa = 10.18FVFHH22 pKa = 6.61EE23 pKa = 4.46VTGKK27 pKa = 10.49AVLEE31 pKa = 3.94QFEE34 pKa = 4.48KK35 pKa = 10.92HH36 pKa = 5.81EE37 pKa = 4.28EE38 pKa = 3.94LHH40 pKa = 5.8FDD42 pKa = 3.34KK43 pKa = 11.22YY44 pKa = 11.24GLSHH48 pKa = 7.1HH49 pKa = 6.84PRR51 pKa = 11.84NFLLIQCALGRR62 pKa = 11.84NYY64 pKa = 10.4RR65 pKa = 11.84PATIVSDD72 pKa = 4.09QLDD75 pKa = 3.62TRR77 pKa = 11.84NACLEE82 pKa = 3.95VLRR85 pKa = 11.84FQPRR89 pKa = 11.84SGPTALVLDD98 pKa = 3.75MAYY101 pKa = 10.43RR102 pKa = 11.84YY103 pKa = 10.76LNLDD107 pKa = 3.47KK108 pKa = 11.37GDD110 pKa = 4.18LLSFYY115 pKa = 10.75ASKK118 pKa = 11.48SNFEE122 pKa = 3.75ATNNVKK128 pKa = 10.73APGTFKK134 pKa = 11.07LPASQLRR141 pKa = 11.84DD142 pKa = 3.42DD143 pKa = 4.7SKK145 pKa = 11.49ILLEE149 pKa = 5.45LIGKK153 pKa = 8.08HH154 pKa = 5.3EE155 pKa = 5.05KK156 pKa = 10.79GSSHH160 pKa = 6.25KK161 pKa = 10.65KK162 pKa = 9.97KK163 pKa = 10.28IEE165 pKa = 3.86SLEE168 pKa = 3.78IRR170 pKa = 11.84SQSVHH175 pKa = 6.04NDD177 pKa = 2.96CVGVITRR184 pKa = 11.84ILSDD188 pKa = 3.79LEE190 pKa = 4.2MNIGDD195 pKa = 4.06PTKK198 pKa = 10.59FDD200 pKa = 3.51QTPTKK205 pKa = 8.68HH206 pKa = 5.89TYY208 pKa = 10.42VDD210 pKa = 4.0TILDD214 pKa = 3.45RR215 pKa = 11.84FYY217 pKa = 11.45KK218 pKa = 10.74NEE220 pKa = 3.86LQRR223 pKa = 11.84YY224 pKa = 8.9LIDD227 pKa = 3.63ILKK230 pKa = 8.66KK231 pKa = 8.38TNAWHH236 pKa = 6.33IGHH239 pKa = 7.73MIRR242 pKa = 11.84DD243 pKa = 3.54ISEE246 pKa = 3.99SLIAHH251 pKa = 7.1AGLKK255 pKa = 9.37RR256 pKa = 11.84SKK258 pKa = 9.38YY259 pKa = 9.27WSIHH263 pKa = 5.79AYY265 pKa = 10.51NDD267 pKa = 3.69GNVLLCILPSKK278 pKa = 10.3SLEE281 pKa = 4.21SAGSFIRR288 pKa = 11.84YY289 pKa = 5.83ITAFRR294 pKa = 11.84LGPGLVDD301 pKa = 4.94AEE303 pKa = 4.35NLDD306 pKa = 4.74SIITDD311 pKa = 3.73EE312 pKa = 4.46AGSWAFSKK320 pKa = 11.14VMSIDD325 pKa = 3.76LNRR328 pKa = 11.84LLALNIAFEE337 pKa = 4.34KK338 pKa = 10.92SLVATATWFQYY349 pKa = 8.65YY350 pKa = 8.96TEE352 pKa = 4.89DD353 pKa = 3.27QGQFPLQHH361 pKa = 7.42AIRR364 pKa = 11.84SVFAYY369 pKa = 10.18HH370 pKa = 6.84FLLSVCQKK378 pKa = 10.0MKK380 pKa = 10.36VCAIFDD386 pKa = 3.67NLRR389 pKa = 11.84YY390 pKa = 9.94LIPAVTSLYY399 pKa = 10.89SGFPSLIEE407 pKa = 3.89NLFEE411 pKa = 4.88RR412 pKa = 11.84PFKK415 pKa = 10.99SSLEE419 pKa = 3.92VYY421 pKa = 9.77IYY423 pKa = 11.46NNIKK427 pKa = 10.46SLLIALAQNNKK438 pKa = 7.96IRR440 pKa = 11.84YY441 pKa = 6.9YY442 pKa = 10.85SKK444 pKa = 10.84VRR446 pKa = 11.84LLGLTVDD453 pKa = 3.77QSTVGASGIYY463 pKa = 9.72PSFLSRR469 pKa = 11.84VIYY472 pKa = 10.15KK473 pKa = 9.92HH474 pKa = 5.43YY475 pKa = 10.35KK476 pKa = 9.16SLISEE481 pKa = 4.13VTTCFFLFEE490 pKa = 4.96KK491 pKa = 10.53GLHH494 pKa = 5.78GNVNEE499 pKa = 4.08EE500 pKa = 4.07AKK502 pKa = 10.26IHH504 pKa = 6.34LEE506 pKa = 4.09TVEE509 pKa = 3.89WALKK513 pKa = 9.63FRR515 pKa = 11.84KK516 pKa = 9.59KK517 pKa = 9.33EE518 pKa = 4.09EE519 pKa = 3.97KK520 pKa = 10.58YY521 pKa = 9.51GTHH524 pKa = 6.09LVEE527 pKa = 3.71NGYY530 pKa = 10.57RR531 pKa = 11.84IFDD534 pKa = 4.35LYY536 pKa = 11.37LNPDD540 pKa = 3.43LVEE543 pKa = 3.99QQLYY547 pKa = 9.03CQDD550 pKa = 3.65VVEE553 pKa = 5.16LGAFEE558 pKa = 5.07LNNMLLSKK566 pKa = 8.9TQVVGNSIINKK577 pKa = 8.65HH578 pKa = 4.91WNLPYY583 pKa = 10.42FSQTRR588 pKa = 11.84NISLKK593 pKa = 10.42GMSGSIQEE601 pKa = 4.87DD602 pKa = 3.78GHH604 pKa = 7.53LSSSVTLIEE613 pKa = 5.26AIRR616 pKa = 11.84YY617 pKa = 6.67LQNSRR622 pKa = 11.84HH623 pKa = 5.58NPSLLQLYY631 pKa = 10.03EE632 pKa = 3.97EE633 pKa = 4.43TRR635 pKa = 11.84SAKK638 pKa = 9.6AQARR642 pKa = 11.84IVRR645 pKa = 11.84KK646 pKa = 7.46YY647 pKa = 10.49QRR649 pKa = 11.84TEE651 pKa = 3.26ADD653 pKa = 2.82RR654 pKa = 11.84GFFITTLPTRR664 pKa = 11.84CRR666 pKa = 11.84LEE668 pKa = 4.86IIEE671 pKa = 5.2DD672 pKa = 4.13YY673 pKa = 11.28YY674 pKa = 11.62DD675 pKa = 5.49AIAKK679 pKa = 7.92NVPEE683 pKa = 4.55EE684 pKa = 4.12YY685 pKa = 9.57ISYY688 pKa = 10.17GGEE691 pKa = 3.58RR692 pKa = 11.84KK693 pKa = 9.39ILNIQQALEE702 pKa = 3.98KK703 pKa = 10.21ALRR706 pKa = 11.84WASGEE711 pKa = 4.11SHH713 pKa = 7.13LEE715 pKa = 3.7LSTGKK720 pKa = 10.09IIPMKK725 pKa = 9.97RR726 pKa = 11.84KK727 pKa = 9.85LMYY730 pKa = 10.47VSADD734 pKa = 3.22ATKK737 pKa = 9.98WSPGDD742 pKa = 3.31NSAKK746 pKa = 9.66FRR748 pKa = 11.84RR749 pKa = 11.84FTAVLHH755 pKa = 6.19NGLRR759 pKa = 11.84DD760 pKa = 3.67DD761 pKa = 4.05KK762 pKa = 11.38LRR764 pKa = 11.84NCVIDD769 pKa = 4.04ALTNIYY775 pKa = 8.69KK776 pKa = 9.69TDD778 pKa = 3.53FFMSRR783 pKa = 11.84KK784 pKa = 8.49LKK786 pKa = 10.56KK787 pKa = 10.26YY788 pKa = 8.42INNMEE793 pKa = 4.1VLDD796 pKa = 4.04DD797 pKa = 3.88HH798 pKa = 7.07VKK800 pKa = 10.57EE801 pKa = 4.25FLSFFPDD808 pKa = 3.59GNSGEE813 pKa = 4.25VHH815 pKa = 6.49GNWLQGNLNKK825 pKa = 10.24CSSLFAVGMSLLFKK839 pKa = 10.59RR840 pKa = 11.84LWKK843 pKa = 10.38EE844 pKa = 3.42LFPEE848 pKa = 4.68LEE850 pKa = 4.61CFFEE854 pKa = 4.35

Molecular weight: 98.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1787

429

854

595.7

68.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.156 ± 0.798

2.518 ± 1.194

5.428 ± 1.054

5.988 ± 0.366

5.092 ± 0.766

5.316 ± 0.238

2.518 ± 0.59

6.547 ± 0.086

6.939 ± 0.204

10.241 ± 1.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.616

4.421 ± 0.7

3.414 ± 0.314

4.197 ± 0.736

5.148 ± 0.544

7.275 ± 0.58

6.212 ± 1.109

5.092 ± 0.13

1.455 ± 0.277

3.973 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski