Cetobacterium ceti
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2511 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4PPN8|A0A1T4PPN8_9FUSO RNA polymerase sigma factor SigS OS=Cetobacterium ceti OX=180163 GN=SAMN02745174_01999 PE=3 SV=1
MM1 pKa = 7.43 EE2 pKa = 4.81 KK3 pKa = 9.21 WRR5 pKa = 11.84 CVICDD10 pKa = 3.51 YY11 pKa = 11.35 VYY13 pKa = 10.82 DD14 pKa = 4.21 PEE16 pKa = 5.27 VGDD19 pKa = 3.88 VDD21 pKa = 4.84 SGIAPGTKK29 pKa = 9.9 FEE31 pKa = 6.11 DD32 pKa = 4.08 IPDD35 pKa = 3.67 DD36 pKa = 4.06 WVCPICSVGKK46 pKa = 9.96 DD47 pKa = 3.3 QFEE50 pKa = 4.47 KK51 pKa = 10.33 MDD53 pKa = 3.42
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.617
ProMoST 3.923
Dawson 3.834
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 1.914
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A1T4KJI6|A0A1T4KJI6_9FUSO Uncharacterized membrane protein OS=Cetobacterium ceti OX=180163 GN=SAMN02745174_00453 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.04 KK3 pKa = 10.29 YY4 pKa = 10.45 RR5 pKa = 11.84 GVLVALILCLFTGCGATIRR24 pKa = 11.84 MSNKK28 pKa = 9.53 KK29 pKa = 9.0 ISRR32 pKa = 11.84 YY33 pKa = 6.67 FTARR37 pKa = 11.84 EE38 pKa = 3.77 ALYY41 pKa = 10.6 SATAKK46 pKa = 10.05 RR47 pKa = 11.84 YY48 pKa = 9.38 NMSNIPLSKK57 pKa = 10.16 ATDD60 pKa = 3.86 NIYY63 pKa = 9.77 YY64 pKa = 9.3 CAWRR68 pKa = 11.84 MDD70 pKa = 3.13 KK71 pKa = 10.55 VRR73 pKa = 11.84 RR74 pKa = 11.84 FLGTPIHH81 pKa = 6.12 VNSWYY86 pKa = 10.3 RR87 pKa = 11.84 SPRR90 pKa = 11.84 VNRR93 pKa = 11.84 KK94 pKa = 9.28 VGGVSHH100 pKa = 7.03 SYY102 pKa = 10.0 HH103 pKa = 5.29 VQGLAVDD110 pKa = 5.85 FYY112 pKa = 10.47 TKK114 pKa = 10.66 KK115 pKa = 10.24 NPKK118 pKa = 9.16 YY119 pKa = 10.39 VYY121 pKa = 10.14 NKK123 pKa = 9.72 LRR125 pKa = 11.84 RR126 pKa = 11.84 SRR128 pKa = 11.84 LSFDD132 pKa = 3.02 QLIYY136 pKa = 10.77 YY137 pKa = 7.98 PRR139 pKa = 11.84 QKK141 pKa = 10.24 RR142 pKa = 11.84 FHH144 pKa = 6.37 ISFRR148 pKa = 11.84 RR149 pKa = 11.84 NIRR152 pKa = 11.84 QEE154 pKa = 3.41 RR155 pKa = 11.84 RR156 pKa = 11.84 QAFVKK161 pKa = 10.54 YY162 pKa = 10.41
Molecular weight: 19.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 10.292
IPC_protein 11.023
Toseland 10.877
ProMoST 10.701
Dawson 11.008
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 11.213
Grimsley 11.067
Solomon 11.14
Lehninger 11.082
Nozaki 10.862
DTASelect 10.76
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.935
Patrickios 10.906
IPC_peptide 11.14
IPC2_peptide 9.853
IPC2.peptide.svr19 7.785
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2511
0
2511
788890
39
5295
314.2
35.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.944 ± 0.057
0.833 ± 0.019
4.929 ± 0.041
8.003 ± 0.065
4.921 ± 0.049
6.954 ± 0.06
1.284 ± 0.019
10.065 ± 0.055
9.906 ± 0.071
9.706 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.474 ± 0.026
6.47 ± 0.061
2.874 ± 0.03
2.065 ± 0.022
3.322 ± 0.037
5.701 ± 0.038
4.795 ± 0.054
5.829 ± 0.049
0.656 ± 0.014
4.267 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here