Human fecal virus Jorvi2
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGC9|A0A220IGC9_9CIRC Uncharacterized protein OS=Human fecal virus Jorvi2 OX=2017081 PE=4 SV=1
MM1 pKa = 7.52 LFDD4 pKa = 5.81 DD5 pKa = 4.36 NDD7 pKa = 4.1 YY8 pKa = 10.69 YY9 pKa = 11.73 DD10 pKa = 5.55 HH11 pKa = 7.49 IFGPDD16 pKa = 3.29 DD17 pKa = 3.39 STNDD21 pKa = 3.21 FNFNFDD27 pKa = 4.25 DD28 pKa = 5.18 FISNEE33 pKa = 4.31 PINDD37 pKa = 4.18 NIDD40 pKa = 4.05 TIPDD44 pKa = 3.56 DD45 pKa = 3.96 TTPEE49 pKa = 3.9 IDD51 pKa = 4.6 SIPPNPLPPPSNSDD65 pKa = 2.93 SVAYY69 pKa = 10.58 YY70 pKa = 10.74 FEE72 pKa = 6.83 DD73 pKa = 3.62 YY74 pKa = 9.51 TQLKK78 pKa = 9.86 EE79 pKa = 4.14 YY80 pKa = 10.71 VKK82 pKa = 10.56 LYY84 pKa = 9.47 YY85 pKa = 10.93 NNVRR89 pKa = 11.84 NHH91 pKa = 6.55 YY92 pKa = 10.15 ILLPVDD98 pKa = 4.8 AIGAIRR104 pKa = 11.84 VDD106 pKa = 3.3 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 SYY112 pKa = 11.48 SNYY115 pKa = 9.98 LIPPSCILLPHH126 pKa = 6.97 PP127 pKa = 4.94
Molecular weight: 14.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.821
IPC2_protein 3.961
IPC_protein 3.961
Toseland 3.732
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.139
Wikipedia 3.961
Rodwell 3.783
Grimsley 3.643
Solomon 3.973
Lehninger 3.923
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.808
EMBOSS 3.961
Sillero 4.088
Patrickios 0.604
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|A0A220IGC8|A0A220IGC8_9CIRC Uncharacterized protein OS=Human fecal virus Jorvi2 OX=2017081 PE=4 SV=1
MM1 pKa = 7.02 ARR3 pKa = 11.84 KK4 pKa = 8.76 YY5 pKa = 10.59 KK6 pKa = 10.16 KK7 pKa = 9.95 VYY9 pKa = 9.93 GSRR12 pKa = 11.84 RR13 pKa = 11.84 PLKK16 pKa = 8.08 TVKK19 pKa = 10.5 YY20 pKa = 10.46 SNEE23 pKa = 3.86 TSNITSSFSITQGGQLNASLVTASSIQGIRR53 pKa = 11.84 KK54 pKa = 9.22 AKK56 pKa = 10.1 NFSLKK61 pKa = 10.04 ILYY64 pKa = 10.14 AGSAPLMFVLVYY76 pKa = 10.11 VPQGQDD82 pKa = 2.44 VKK84 pKa = 11.13 AITRR88 pKa = 11.84 GTYY91 pKa = 7.3 TAPASLYY98 pKa = 10.16 EE99 pKa = 4.23 PNQNVIMSGYY109 pKa = 9.41 IVPNNSQAQTFRR121 pKa = 11.84 TRR123 pKa = 11.84 LARR126 pKa = 11.84 NLNSGDD132 pKa = 3.92 SIQIAICCAGASDD145 pKa = 4.18 TVTDD149 pKa = 4.12 ANIGISLNYY158 pKa = 10.39 AITFF162 pKa = 3.98
Molecular weight: 17.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.152
IPC2_protein 9.619
IPC_protein 9.75
Toseland 9.911
ProMoST 9.736
Dawson 10.189
Bjellqvist 9.911
Wikipedia 10.394
Rodwell 10.482
Grimsley 10.292
Solomon 10.218
Lehninger 10.175
Nozaki 9.94
DTASelect 9.911
Thurlkill 10.014
EMBOSS 10.335
Sillero 10.101
Patrickios 7.497
IPC_peptide 10.204
IPC2_peptide 8.756
IPC2.peptide.svr19 8.54
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
938
116
312
187.6
21.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.691 ± 1.05
1.173 ± 0.458
6.397 ± 1.684
5.224 ± 1.211
4.904 ± 0.677
4.264 ± 0.865
1.599 ± 0.477
7.676 ± 0.716
6.503 ± 1.659
7.889 ± 0.343
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.706 ± 0.401
7.569 ± 0.517
5.011 ± 1.313
2.985 ± 0.574
3.945 ± 0.464
7.783 ± 1.232
7.783 ± 1.657
4.797 ± 0.562
2.026 ± 1.006
6.077 ± 0.609
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here