Magnaporthe grisea (Crabgrass-specific blast fungus) (Pyricularia grisea)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P8B9F8|A0A6P8B9F8_MAGGR uncharacterized protein OS=Magnaporthe grisea OX=148305 GN=PgNI_02904 PE=3 SV=1
MM1 pKa = 7.58PNKK4 pKa = 10.39SQMLLVLAAALVPLAWGGSSSSILRR29 pKa = 11.84PRR31 pKa = 11.84QQGTQDD37 pKa = 3.22MPRR40 pKa = 11.84PTFGPVPWGEE50 pKa = 3.7QLGRR54 pKa = 11.84DD55 pKa = 4.1DD56 pKa = 5.18FPCARR61 pKa = 11.84AGQIAFTFEE70 pKa = 5.2DD71 pKa = 4.98GPSPYY76 pKa = 9.79TPSILNTLSQYY87 pKa = 11.39NIIASFFMTGSFEE100 pKa = 5.25DD101 pKa = 3.94GGLAQPRR108 pKa = 11.84YY109 pKa = 9.19HH110 pKa = 6.41GLARR114 pKa = 11.84FMYY117 pKa = 9.86NQGHH121 pKa = 6.88LLGSHH126 pKa = 6.63SYY128 pKa = 10.44SHH130 pKa = 7.93DD131 pKa = 3.47DD132 pKa = 3.23LRR134 pKa = 11.84SFTEE138 pKa = 3.95EE139 pKa = 3.66EE140 pKa = 3.98VRR142 pKa = 11.84ADD144 pKa = 3.94LLEE147 pKa = 4.84LEE149 pKa = 4.41ATFADD154 pKa = 3.91VLGVVPTYY162 pKa = 10.36FRR164 pKa = 11.84APFTEE169 pKa = 4.84CDD171 pKa = 3.49VGDD174 pKa = 4.08CLVNIRR180 pKa = 11.84PLVYY184 pKa = 10.41HH185 pKa = 6.51IVDD188 pKa = 3.66YY189 pKa = 11.47NIDD192 pKa = 3.62SFDD195 pKa = 3.86SYY197 pKa = 11.64EE198 pKa = 4.19LADD201 pKa = 4.23PADD204 pKa = 3.38LVAFFRR210 pKa = 11.84EE211 pKa = 4.25TMEE214 pKa = 4.36NADD217 pKa = 4.2PAVASYY223 pKa = 10.44IVRR226 pKa = 11.84FHH228 pKa = 6.71DD229 pKa = 3.83TEE231 pKa = 4.19EE232 pKa = 4.11VTANGLLEE240 pKa = 4.16EE241 pKa = 5.47FILIAATRR249 pKa = 11.84GFEE252 pKa = 3.95FVTVGEE258 pKa = 4.74CLGDD262 pKa = 3.77DD263 pKa = 3.62PGNFYY268 pKa = 10.97RR269 pKa = 11.84NPQTGNALGNGNEE282 pKa = 4.69PPPSPDD288 pKa = 3.18PSSSTEE294 pKa = 3.85STAVSASTSVSSSSVDD310 pKa = 3.51LTSSAIEE317 pKa = 4.02TTGTPEE323 pKa = 3.41ITGNPDD329 pKa = 2.92SMVSDD334 pKa = 4.11TQTSTATDD342 pKa = 3.4QEE344 pKa = 4.52VTITLDD350 pKa = 3.14TLTQEE355 pKa = 4.53TPSVASTTIDD365 pKa = 3.17TAGSDD370 pKa = 3.9DD371 pKa = 3.72PTITEE376 pKa = 4.41TQTITQTEE384 pKa = 4.5TDD386 pKa = 3.37TADD389 pKa = 3.46FPGATTTPAVAGTSDD404 pKa = 3.58MVTTSDD410 pKa = 3.47STPTITEE417 pKa = 3.67ISVSSVADD425 pKa = 3.45VTSSEE430 pKa = 4.25EE431 pKa = 4.23TTSDD435 pKa = 3.08ASQTALEE442 pKa = 4.38SSLDD446 pKa = 3.67ATSSLVTTEE455 pKa = 4.68LASQTTAAATSEE467 pKa = 4.4EE468 pKa = 4.28PSASHH473 pKa = 6.7SLITMSIISEE483 pKa = 4.37TTSTGFDD490 pKa = 3.48TPSSSVVDD498 pKa = 4.14GQTTSGAPSDD508 pKa = 4.98DD509 pKa = 4.81GITTSTSTAPTSEE522 pKa = 4.3VPTSEE527 pKa = 3.87VTDD530 pKa = 3.5TGFCTSTASFAGSLVPSDD548 pKa = 3.86TVSDD552 pKa = 3.46ISTPTATITTTCSTATCTTAATTSADD578 pKa = 3.27EE579 pKa = 4.3TAPSVTSTDD588 pKa = 3.19SGSQSSAATNFPHH601 pKa = 7.53SIITVVTKK609 pKa = 10.07TLDD612 pKa = 3.3STTQDD617 pKa = 2.9AQTNSQNTPSPSSSISPSDD636 pKa = 3.63VTPLPLSSSTEE647 pKa = 3.66IRR649 pKa = 11.84DD650 pKa = 3.3SSTIEE655 pKa = 4.11EE656 pKa = 4.48DD657 pKa = 3.46TSSATAMPNSASYY670 pKa = 7.92TTFPHH675 pKa = 5.77QFSSAEE681 pKa = 4.09VQSTGAASTTINDD694 pKa = 3.13QDD696 pKa = 3.96TNEE699 pKa = 4.2TANPATHH706 pKa = 5.42THH708 pKa = 6.12YY709 pKa = 10.95GSSTTITHH717 pKa = 6.17TAGATDD723 pKa = 4.61CPTATAANDD732 pKa = 4.02DD733 pKa = 4.26AQQSPPTPTEE743 pKa = 3.92TPQNGGGPGQEE754 pKa = 3.81TSSRR758 pKa = 11.84CGGCQDD764 pKa = 3.51GVVGGEE770 pKa = 3.94GSFGNWLTVTRR781 pKa = 11.84SSAIGRR787 pKa = 11.84DD788 pKa = 3.84DD789 pKa = 3.92LQSAGTSAGGISAGVGIQTYY809 pKa = 9.8VISSAQPPSASVAGQGAGEE828 pKa = 4.34LPSGAEE834 pKa = 3.89TGGVGGSSAVPTRR847 pKa = 11.84VVGAGGFAVGASGCAAVFAAVMAWMVLL874 pKa = 3.56

Molecular weight: 89.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P8BIR3|A0A6P8BIR3_MAGGR Mannosyltransferase OS=Magnaporthe grisea OX=148305 GN=PgNI_01330 PE=3 SV=1
MM1 pKa = 7.26VFQSRR6 pKa = 11.84LRR8 pKa = 11.84FFPRR12 pKa = 11.84PGSHH16 pKa = 6.49KK17 pKa = 10.62SFRR20 pKa = 11.84TKK22 pKa = 10.45QKK24 pKa = 9.84LAKK27 pKa = 9.55AQKK30 pKa = 8.59QNRR33 pKa = 11.84PIPQWIRR40 pKa = 11.84LRR42 pKa = 11.84TGNTIRR48 pKa = 11.84YY49 pKa = 5.79NAKK52 pKa = 8.89RR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.14WRR57 pKa = 11.84KK58 pKa = 7.41TRR60 pKa = 11.84LGII63 pKa = 4.46

Molecular weight: 7.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12446

9

12455

5706119

31

6480

458.1

50.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.221 ± 0.027

1.252 ± 0.01

5.761 ± 0.019

5.886 ± 0.025

3.513 ± 0.013

7.322 ± 0.024

2.325 ± 0.011

4.502 ± 0.015

4.735 ± 0.022

8.595 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.008

3.6 ± 0.012

6.225 ± 0.026

4.059 ± 0.02

6.288 ± 0.019

8.225 ± 0.031

5.984 ± 0.022

6.289 ± 0.018

1.412 ± 0.009

2.547 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski