Rhizopogon vinicolor AM-OR11-026

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Boletales; Suillineae; Rhizopogonaceae; Rhizopogon; Rhizopogon vinicolor

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B7N717|A0A1B7N717_9AGAM E3 ubiquitin-protein ligase listerin OS=Rhizopogon vinicolor AM-OR11-026 OX=1314800 GN=K503DRAFT_864487 PE=3 SV=1
MM1 pKa = 7.2FNIVNSAFSAVATVFQMIVSYY22 pKa = 9.19MIIALCFIGLMLIDD36 pKa = 5.9LIDD39 pKa = 3.96EE40 pKa = 4.37VSLQVSPASEE50 pKa = 4.12KK51 pKa = 10.54VVCSCVTDD59 pKa = 4.26CPHH62 pKa = 5.8TEE64 pKa = 3.72ARR66 pKa = 11.84EE67 pKa = 3.97EE68 pKa = 4.18LLVAPADD75 pKa = 3.55IDD77 pKa = 3.8EE78 pKa = 4.56VEE80 pKa = 3.84LGRR83 pKa = 11.84RR84 pKa = 11.84YY85 pKa = 10.31VEE87 pKa = 4.14STDD90 pKa = 3.83DD91 pKa = 3.77SDD93 pKa = 3.74VSRR96 pKa = 11.84SFEE99 pKa = 4.0FDD101 pKa = 2.71VSTFSTFEE109 pKa = 4.15SLLSSSPSFIEE120 pKa = 4.88LGSAPWAEE128 pKa = 4.87LDD130 pKa = 3.5TLEE133 pKa = 5.08CVTSSPHH140 pKa = 6.72SPTTTMDD147 pKa = 3.35AADD150 pKa = 3.43ISRR153 pKa = 11.84SFEE156 pKa = 3.83LDD158 pKa = 2.88ASVVGTFEE166 pKa = 4.58FLLSSSPSFIEE177 pKa = 5.36LGSAPCDD184 pKa = 3.42EE185 pKa = 5.72LDD187 pKa = 3.55TPRR190 pKa = 11.84CVTSWMDD197 pKa = 3.03GADD200 pKa = 3.06ISRR203 pKa = 11.84SFEE206 pKa = 4.2LNASVVGTFEE216 pKa = 4.58FLLSSSPSFIEE227 pKa = 5.36LGSAPCDD234 pKa = 3.42EE235 pKa = 5.72LDD237 pKa = 3.61TPRR240 pKa = 11.84CVAFPMDD247 pKa = 4.45DD248 pKa = 3.64ANISRR253 pKa = 11.84SFEE256 pKa = 4.38LNASMVGTFEE266 pKa = 4.67SLLSSSPSFIEE277 pKa = 4.67LGSVPCDD284 pKa = 3.4EE285 pKa = 6.02LDD287 pKa = 3.64TPQCVASSVDD297 pKa = 3.89DD298 pKa = 4.35ADD300 pKa = 3.65ISQSFEE306 pKa = 4.09LDD308 pKa = 3.22ASSLGAFEE316 pKa = 5.12SLLPSSPSFLEE327 pKa = 4.25LGSAPSVEE335 pKa = 4.96LDD337 pKa = 3.38TPEE340 pKa = 4.69CVSSSPYY347 pKa = 10.85LPTTTTMDD355 pKa = 4.08DD356 pKa = 3.57NTSVSLSMFDD366 pKa = 3.24VDD368 pKa = 5.69RR369 pKa = 11.84FPTLISSSTSFVALGEE385 pKa = 4.3ASCSYY390 pKa = 11.19YY391 pKa = 10.77LDD393 pKa = 3.88TSHH396 pKa = 7.01HH397 pKa = 5.58VTSSAPSPNSSVSLSVFDD415 pKa = 5.03LEE417 pKa = 4.93RR418 pKa = 11.84FPSLICPSSSFRR430 pKa = 11.84ALASASRR437 pKa = 11.84RR438 pKa = 11.84SSTASSFSLSTSINDD453 pKa = 3.57ADD455 pKa = 3.98VTISLSSSFSKK466 pKa = 10.98YY467 pKa = 9.89PSLLSSSPSFIALGSVLSS485 pKa = 3.92

Molecular weight: 51.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B7N1U5|A0A1B7N1U5_9AGAM Uncharacterized protein OS=Rhizopogon vinicolor AM-OR11-026 OX=1314800 GN=K503DRAFT_815831 PE=4 SV=1
MM1 pKa = 7.34TRR3 pKa = 11.84TGSPPSRR10 pKa = 11.84SLMILIAMMIKK21 pKa = 10.32SRR23 pKa = 11.84MTVDD27 pKa = 3.53TSHH30 pKa = 7.16TSPTRR35 pKa = 11.84TTTRR39 pKa = 11.84RR40 pKa = 11.84IRR42 pKa = 11.84RR43 pKa = 11.84MIIRR47 pKa = 11.84KK48 pKa = 7.05TSIRR52 pKa = 11.84RR53 pKa = 11.84TIKK56 pKa = 9.94KK57 pKa = 8.27RR58 pKa = 11.84TMISGTRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84TTKK70 pKa = 10.17RR71 pKa = 11.84RR72 pKa = 11.84TKK74 pKa = 10.06RR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84TITRR81 pKa = 11.84STMMNGMTTRR91 pKa = 11.84TMTRR95 pKa = 11.84RR96 pKa = 11.84MTRR99 pKa = 11.84RR100 pKa = 11.84MTRR103 pKa = 11.84RR104 pKa = 11.84XXXXTRR110 pKa = 11.84RR111 pKa = 11.84MTRR114 pKa = 11.84RR115 pKa = 11.84MTRR118 pKa = 11.84RR119 pKa = 11.84TTRR122 pKa = 11.84RR123 pKa = 11.84TTRR126 pKa = 11.84KK127 pKa = 6.45ITRR130 pKa = 11.84RR131 pKa = 11.84MTRR134 pKa = 11.84RR135 pKa = 11.84TMMNGTMTRR144 pKa = 11.84RR145 pKa = 11.84MMNGTTTRR153 pKa = 11.84RR154 pKa = 11.84TKK156 pKa = 9.05MRR158 pKa = 11.84KK159 pKa = 8.44IRR161 pKa = 11.84RR162 pKa = 11.84TMTRR166 pKa = 11.84TKK168 pKa = 9.84MIRR171 pKa = 11.84RR172 pKa = 11.84TIMNGTTTTRR182 pKa = 11.84TRR184 pKa = 11.84TRR186 pKa = 11.84RR187 pKa = 11.84TRR189 pKa = 11.84RR190 pKa = 11.84TKK192 pKa = 10.4RR193 pKa = 11.84RR194 pKa = 11.84MMINGTRR201 pKa = 11.84IKK203 pKa = 10.71SGTSLTATLLSNLRR217 pKa = 11.84VIDD220 pKa = 4.13LPKK223 pKa = 10.88KK224 pKa = 10.59NFTCVISYY232 pKa = 7.51TLL234 pKa = 3.54

Molecular weight: 27.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14447

0

14447

5225860

49

5049

361.7

40.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.113 ± 0.019

1.442 ± 0.01

5.545 ± 0.015

5.643 ± 0.022

3.822 ± 0.014

6.25 ± 0.021

2.721 ± 0.011

5.241 ± 0.016

4.371 ± 0.019

9.383 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.008

3.409 ± 0.011

6.239 ± 0.029

3.753 ± 0.011

6.134 ± 0.019

8.941 ± 0.03

6.073 ± 0.014

6.469 ± 0.016

1.492 ± 0.009

2.723 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski