Macacine betaherpesvirus 3 (Rhesus cytomegalovirus)
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 174 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3WEZ2|I3WEZ2_9BETA Rh150 OS=Macacine betaherpesvirus 3 OX=47929 PE=4 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 WLAMRR7 pKa = 11.84 WLLLIVATSAKK18 pKa = 10.29 ASSTTAGNTSTPTASTITSTVTTASTTNMSTPSPASSPATTNTTTSSPSPKK69 pKa = 10.11 SSTSSTNTTITTTGTASATTPKK91 pKa = 10.4 SSTSVTSSPANTTSTSTKK109 pKa = 7.5 PTNVSSPTVSSPASNTSTTTSTTSTVNGTNSTTPKK144 pKa = 10.21 TNTTSLPNTTADD156 pKa = 3.49 MNVTTTEE163 pKa = 4.5 PYY165 pKa = 10.96 NSTGYY170 pKa = 11.03 EE171 pKa = 3.7 NVTINITTPSPYY183 pKa = 10.32 EE184 pKa = 3.56 PLQIVDD190 pKa = 4.74 LCNEE194 pKa = 4.43 TISIVFKK201 pKa = 11.15 DD202 pKa = 3.83 PGEE205 pKa = 4.03 EE206 pKa = 3.86 DD207 pKa = 5.01 DD208 pKa = 4.31 INEE211 pKa = 3.87 SSEE214 pKa = 3.95 YY215 pKa = 10.89 SNEE218 pKa = 3.92 EE219 pKa = 3.84 PEE221 pKa = 4.52 TNSEE225 pKa = 3.81 EE226 pKa = 4.08 DD227 pKa = 3.73 TYY229 pKa = 11.23 FPQSPGYY236 pKa = 7.88 TLTYY240 pKa = 9.43 DD241 pKa = 3.78 TEE243 pKa = 4.17 DD244 pKa = 3.27 TIYY247 pKa = 10.65 FQATCDD253 pKa = 4.87 RR254 pKa = 11.84 NDD256 pKa = 3.19 TYY258 pKa = 11.81 QNITSCDD265 pKa = 3.63 YY266 pKa = 10.58 TNKK269 pKa = 10.2 SVNSWSTVTSVSFFPPTLTPCHH291 pKa = 6.64 KK292 pKa = 9.58 PLAIIKK298 pKa = 9.74 IGNDD302 pKa = 3.25 SLVVSASATSNLVDD316 pKa = 5.76 AIYY319 pKa = 10.99 KK320 pKa = 10.49 LLGLPDD326 pKa = 3.8 VNSDD330 pKa = 3.77 FLNQLGRR337 pKa = 11.84 YY338 pKa = 8.56 HH339 pKa = 7.47 PIILQGQIEE348 pKa = 4.25 YY349 pKa = 10.26 RR350 pKa = 11.84 DD351 pKa = 3.61 WYY353 pKa = 7.62 TTEE356 pKa = 3.67
Molecular weight: 37.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.996
IPC2_protein 4.253
IPC_protein 4.202
Toseland 4.012
ProMoST 4.291
Dawson 4.164
Bjellqvist 4.342
Wikipedia 4.05
Rodwell 4.024
Grimsley 3.923
Solomon 4.151
Lehninger 4.113
Nozaki 4.266
DTASelect 4.457
Thurlkill 4.037
EMBOSS 4.062
Sillero 4.317
Patrickios 0.947
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.217
Protein with the highest isoelectric point:
>tr|I3WF96|I3WF96_9BETA Rh228.1 OS=Macacine betaherpesvirus 3 OX=47929 PE=4 SV=1
MM1 pKa = 6.63 WHH3 pKa = 7.65 AITVHH8 pKa = 5.76 TPSGVASAARR18 pKa = 11.84 AVNNALSDD26 pKa = 3.8 LVCRR30 pKa = 11.84 ISMLCARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 AVTQEE46 pKa = 3.52 TATAGSSEE54 pKa = 3.83 ARR56 pKa = 11.84 QAEE59 pKa = 4.37 QKK61 pKa = 10.23 QEE63 pKa = 3.93 QPQTSSSTSFLGRR76 pKa = 11.84 ALVLVGQQLLAANQAACPPPAWLWKK101 pKa = 10.35
Molecular weight: 10.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.326
IPC_protein 10.218
Toseland 10.847
ProMoST 10.95
Dawson 10.862
Bjellqvist 10.672
Wikipedia 11.155
Rodwell 10.847
Grimsley 10.877
Solomon 11.155
Lehninger 11.111
Nozaki 10.877
DTASelect 10.657
Thurlkill 10.818
EMBOSS 11.272
Sillero 10.833
Patrickios 10.716
IPC_peptide 11.169
IPC2_peptide 10.218
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
174
0
174
63229
73
2177
363.4
41.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.587 ± 0.145
2.649 ± 0.109
4.76 ± 0.139
5.183 ± 0.167
4.142 ± 0.126
4.552 ± 0.127
3.049 ± 0.092
5.21 ± 0.146
3.861 ± 0.133
9.87 ± 0.201
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.603 ± 0.079
4.332 ± 0.128
5.445 ± 0.192
3.924 ± 0.134
6.404 ± 0.169
7.947 ± 0.162
7.435 ± 0.272
6.94 ± 0.155
1.397 ± 0.084
3.71 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here