Bandicoot papillomatosis carcinomatosis virus type 1
Average proteome isoelectric point is 5.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9QH33|A9QH33_9PAPI Putative small T antigen OS=Bandicoot papillomatosis carcinomatosis virus type 1 OX=479058 PE=4 SV=1
MM1 pKa = 7.99 DD2 pKa = 4.52 NSDD5 pKa = 3.62 YY6 pKa = 11.73 LEE8 pKa = 3.87 FLKK11 pKa = 11.28 LIGCDD16 pKa = 3.09 TCDD19 pKa = 3.45 GPEE22 pKa = 4.29 NIKK25 pKa = 10.54 KK26 pKa = 10.2 SYY28 pKa = 10.51 RR29 pKa = 11.84 KK30 pKa = 9.35 AALKK34 pKa = 7.61 MHH36 pKa = 7.13 PDD38 pKa = 3.3 KK39 pKa = 11.49 GGSTNAMKK47 pKa = 10.72 RR48 pKa = 11.84 LNEE51 pKa = 4.01 LNQSYY56 pKa = 10.61 LEE58 pKa = 4.12 YY59 pKa = 10.9 GPPQNKK65 pKa = 9.25 RR66 pKa = 11.84 YY67 pKa = 9.98 RR68 pKa = 11.84 PTPQYY73 pKa = 11.03 EE74 pKa = 3.98 EE75 pKa = 5.72 DD76 pKa = 3.73 LFCYY80 pKa = 10.11 EE81 pKa = 5.04 SEE83 pKa = 4.87 GDD85 pKa = 5.07 DD86 pKa = 4.48 EE87 pKa = 6.35 PDD89 pKa = 3.08 TCSYY93 pKa = 10.69 NRR95 pKa = 11.84 SDD97 pKa = 3.38 SGFSEE102 pKa = 5.25 GTCPTPDD109 pKa = 3.28 PSGFSASNVSFHH121 pKa = 6.09 QSCKK125 pKa = 9.41 NVKK128 pKa = 9.87 LLLDD132 pKa = 3.28 ITEE135 pKa = 4.44 TLGHH139 pKa = 6.34 GRR141 pKa = 11.84 NCAHH145 pKa = 6.49 TLEE148 pKa = 6.06 AIDD151 pKa = 4.49 KK152 pKa = 8.84 NNRR155 pKa = 11.84 MDD157 pKa = 3.44 WEE159 pKa = 4.41 LFDD162 pKa = 4.66 TFCSTPGNHH171 pKa = 7.0 HH172 pKa = 6.62 LTLMFIDD179 pKa = 3.86 HH180 pKa = 7.48 CYY182 pKa = 10.13 MKK184 pKa = 10.83 LKK186 pKa = 10.29 DD187 pKa = 3.95 WQWSLMNFVNYY198 pKa = 9.56 GPPYY202 pKa = 10.31 LEE204 pKa = 4.85 EE205 pKa = 4.52 IKK207 pKa = 10.62 EE208 pKa = 4.02 AANGIDD214 pKa = 3.43 WSEE217 pKa = 4.2 LDD219 pKa = 5.57 DD220 pKa = 4.84 MIFSS224 pKa = 4.21
Molecular weight: 25.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.482
IPC2_protein 4.673
IPC_protein 4.622
Toseland 4.469
ProMoST 4.749
Dawson 4.584
Bjellqvist 4.724
Wikipedia 4.469
Rodwell 4.469
Grimsley 4.38
Solomon 4.584
Lehninger 4.546
Nozaki 4.698
DTASelect 4.889
Thurlkill 4.482
EMBOSS 4.495
Sillero 4.749
Patrickios 2.956
IPC_peptide 4.584
IPC2_peptide 4.736
IPC2.peptide.svr19 4.695
Protein with the highest isoelectric point:
>tr|A9QH33|A9QH33_9PAPI Putative small T antigen OS=Bandicoot papillomatosis carcinomatosis virus type 1 OX=479058 PE=4 SV=1
MM1 pKa = 7.93 AIWQPNGKK9 pKa = 9.93 VYY11 pKa = 10.68 LPPSAPVARR20 pKa = 11.84 ILNTEE25 pKa = 4.2 EE26 pKa = 4.02 YY27 pKa = 9.62 IQRR30 pKa = 11.84 TSLFYY35 pKa = 10.49 HH36 pKa = 7.0 ANTEE40 pKa = 3.95 RR41 pKa = 11.84 LLTVGHH47 pKa = 7.3 PYY49 pKa = 10.5 FEE51 pKa = 5.06 IKK53 pKa = 10.5 DD54 pKa = 3.74 KK55 pKa = 11.33 LDD57 pKa = 3.24 QTKK60 pKa = 10.2 IGVPKK65 pKa = 10.55 VSANQYY71 pKa = 8.75 RR72 pKa = 11.84 VFRR75 pKa = 11.84 IKK77 pKa = 10.92 LPDD80 pKa = 4.01 PNRR83 pKa = 11.84 FALGDD88 pKa = 3.46 KK89 pKa = 10.95 SFFDD93 pKa = 4.04 PEE95 pKa = 4.09 KK96 pKa = 10.79 EE97 pKa = 3.83 RR98 pKa = 11.84 LVWGIRR104 pKa = 11.84 GLEE107 pKa = 4.05 VEE109 pKa = 5.01 RR110 pKa = 11.84 GQPLNVGSTGNVLYY124 pKa = 11.1 NKK126 pKa = 10.13 FKK128 pKa = 10.46 DD129 pKa = 3.7 TEE131 pKa = 4.25 NPNNYY136 pKa = 9.73 AKK138 pKa = 10.8 EE139 pKa = 4.25 GDD141 pKa = 3.96 DD142 pKa = 3.31 EE143 pKa = 4.42 RR144 pKa = 11.84 QNVSHH149 pKa = 7.54 DD150 pKa = 3.82 PKK152 pKa = 11.1 QMQLLLVGCKK162 pKa = 9.42 PATGEE167 pKa = 3.82 HH168 pKa = 6.02 WDD170 pKa = 3.69 AAKK173 pKa = 9.52 TCADD177 pKa = 3.85 RR178 pKa = 11.84 PLNKK182 pKa = 9.83 GDD184 pKa = 4.24 CPPLEE189 pKa = 4.22 LVNTIIEE196 pKa = 4.99 DD197 pKa = 3.79 GDD199 pKa = 3.82 MMDD202 pKa = 4.03 IGYY205 pKa = 11.06 GNMNFKK211 pKa = 10.8 ALTGNKK217 pKa = 9.52 SDD219 pKa = 3.65 VPLEE223 pKa = 4.02 ISRR226 pKa = 11.84 STTKK230 pKa = 10.82 YY231 pKa = 10.2 PDD233 pKa = 3.73 FLKK236 pKa = 8.54 MTSDD240 pKa = 3.44 QYY242 pKa = 11.69 GDD244 pKa = 3.6 SLFFYY249 pKa = 9.21 TKK251 pKa = 10.41 KK252 pKa = 9.25 EE253 pKa = 3.85 STYY256 pKa = 11.18 ARR258 pKa = 11.84 HH259 pKa = 5.47 FWTRR263 pKa = 11.84 GGTSGDD269 pKa = 4.63 PIPEE273 pKa = 4.32 DD274 pKa = 3.79 LFVAPTDD281 pKa = 3.54 NQKK284 pKa = 9.98 ILGSANYY291 pKa = 7.34 FTVPSGSLINSEE303 pKa = 3.8 AQLFNRR309 pKa = 11.84 PYY311 pKa = 8.49 WLQRR315 pKa = 11.84 ATGQNNGICWNNDD328 pKa = 2.88 LFITIVDD335 pKa = 3.46 NTRR338 pKa = 11.84 NCNFTISVFNKK349 pKa = 9.52 ADD351 pKa = 3.47 EE352 pKa = 4.72 SQGQTYY358 pKa = 10.18 KK359 pKa = 10.69 SSNYY363 pKa = 9.69 SVYY366 pKa = 10.28 LRR368 pKa = 11.84 HH369 pKa = 6.71 VEE371 pKa = 3.92 EE372 pKa = 5.04 FEE374 pKa = 4.85 LNFIFEE380 pKa = 4.24 ICKK383 pKa = 10.17 VPLKK387 pKa = 10.58 PEE389 pKa = 3.88 VLAHH393 pKa = 6.38 INAMDD398 pKa = 3.85 PRR400 pKa = 11.84 ILEE403 pKa = 4.23 DD404 pKa = 3.06 WSLGFIPSNNITLEE418 pKa = 3.66 DD419 pKa = 3.77 RR420 pKa = 11.84 YY421 pKa = 11.14 RR422 pKa = 11.84 FINSLATRR430 pKa = 11.84 CPDD433 pKa = 3.48 KK434 pKa = 10.29 EE435 pKa = 4.59 TPKK438 pKa = 10.71 EE439 pKa = 3.96 KK440 pKa = 10.25 EE441 pKa = 4.08 DD442 pKa = 3.91 PYY444 pKa = 11.31 KK445 pKa = 11.31 SNTFWNIDD453 pKa = 3.39 LEE455 pKa = 4.32 DD456 pKa = 4.2 RR457 pKa = 11.84 MSSEE461 pKa = 4.26 LDD463 pKa = 3.17 QYY465 pKa = 11.36 PLGRR469 pKa = 11.84 KK470 pKa = 8.57 FLYY473 pKa = 9.87 QSSISTINTIAKK485 pKa = 9.0 PRR487 pKa = 11.84 TGKK490 pKa = 10.37 RR491 pKa = 11.84 KK492 pKa = 8.56 STTSPTTKK500 pKa = 9.33 TKK502 pKa = 10.22 RR503 pKa = 11.84 RR504 pKa = 11.84 KK505 pKa = 9.36
Molecular weight: 57.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.605
IPC2_protein 6.825
IPC_protein 6.883
Toseland 6.751
ProMoST 7.512
Dawson 7.512
Bjellqvist 7.541
Wikipedia 7.439
Rodwell 7.512
Grimsley 6.825
Solomon 7.571
Lehninger 7.585
Nozaki 7.805
DTASelect 7.673
Thurlkill 7.717
EMBOSS 7.775
Sillero 7.936
Patrickios 4.342
IPC_peptide 7.571
IPC2_peptide 7.0
IPC2.peptide.svr19 7.138
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1198
224
505
399.3
45.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.756 ± 0.321
1.503 ± 0.652
7.095 ± 0.483
6.678 ± 0.316
4.341 ± 0.352
6.594 ± 0.513
1.753 ± 0.354
6.427 ± 0.961
6.093 ± 0.702
8.097 ± 0.21
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.836 ± 0.436
6.594 ± 0.584
7.346 ± 0.707
2.922 ± 0.253
4.758 ± 0.502
7.012 ± 0.31
7.763 ± 0.76
4.508 ± 0.878
1.085 ± 0.384
3.84 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here