Parolsenella catena
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1584 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G9JXY2|A0A3G9JXY2_9ACTN RNA polymerase sigma-37 factor OS=Parolsenella catena OX=2003188 GN=sigB PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 6.32 PIIISVDD9 pKa = 3.03 EE10 pKa = 4.25 RR11 pKa = 11.84 LAGAGSVLPVAGHH24 pKa = 7.01 IDD26 pKa = 3.78 DD27 pKa = 5.53 DD28 pKa = 4.84 SYY30 pKa = 12.17 SVGDD34 pKa = 3.68 HH35 pKa = 7.2 DD36 pKa = 4.84 FRR38 pKa = 11.84 LPGGIDD44 pKa = 3.49 YY45 pKa = 11.23 DD46 pKa = 4.54 LVLTNAGEE54 pKa = 4.76 GILASGIVRR63 pKa = 11.84 AHH65 pKa = 6.11 VLGTCDD71 pKa = 3.37 RR72 pKa = 11.84 CLEE75 pKa = 4.22 DD76 pKa = 4.85 AEE78 pKa = 4.46 FDD80 pKa = 3.38 IASEE84 pKa = 3.87 VDD86 pKa = 3.47 EE87 pKa = 4.7 YY88 pKa = 11.74 FLFEE92 pKa = 4.55 APAEE96 pKa = 4.13 EE97 pKa = 4.98 DD98 pKa = 3.61 LSDD101 pKa = 5.94 DD102 pKa = 3.64 EE103 pKa = 6.06 DD104 pKa = 3.85 EE105 pKa = 4.7 VDD107 pKa = 4.43 FSLVGEE113 pKa = 4.4 DD114 pKa = 3.35 HH115 pKa = 6.96 TIDD118 pKa = 3.4 LAEE121 pKa = 5.5 PIMAAIVMEE130 pKa = 4.39 TPFVVLCRR138 pKa = 11.84 EE139 pKa = 4.22 DD140 pKa = 4.47 CKK142 pKa = 11.13 GLCPHH147 pKa = 7.15 CGANLNEE154 pKa = 4.51 GDD156 pKa = 4.28 CGCAEE161 pKa = 4.21 KK162 pKa = 10.08 MASVDD167 pKa = 3.25 AATGPFAALAGLRR180 pKa = 11.84 LDD182 pKa = 5.36 GEE184 pKa = 5.23 DD185 pKa = 4.04 GDD187 pKa = 4.63 SAQDD191 pKa = 3.19
Molecular weight: 20.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.643
IPC_protein 3.668
Toseland 3.439
ProMoST 3.808
Dawson 3.656
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.49
Grimsley 3.35
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.012
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.783
Patrickios 0.846
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.725
Protein with the highest isoelectric point:
>tr|A0A3G9K9Q8|A0A3G9K9Q8_9ACTN Molybdate ABC transporter substrate-binding protein OS=Parolsenella catena OX=2003188 GN=modA PE=3 SV=1
MM1 pKa = 7.19 TARR4 pKa = 11.84 FTVIVIAKK12 pKa = 7.22 MWNSILSLSFMGAAPFRR29 pKa = 11.84 PPRR32 pKa = 11.84 QAASVRR38 pKa = 11.84 RR39 pKa = 11.84 FLAIRR44 pKa = 11.84 GAAKK48 pKa = 10.43 SLFGFHH54 pKa = 7.22 AARR57 pKa = 11.84 GRR59 pKa = 11.84 LPRR62 pKa = 11.84 SRR64 pKa = 11.84 APLASPDD71 pKa = 3.63 GGPFLASPVPLAATAGRR88 pKa = 11.84 PRR90 pKa = 11.84 PP91 pKa = 3.68
Molecular weight: 9.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.018
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.126
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1584
0
1584
527162
42
2467
332.8
36.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.944 ± 0.086
1.622 ± 0.027
6.356 ± 0.047
6.689 ± 0.053
3.266 ± 0.034
8.615 ± 0.059
2.023 ± 0.029
4.608 ± 0.049
3.445 ± 0.048
9.232 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.593 ± 0.028
2.743 ± 0.037
4.331 ± 0.04
2.749 ± 0.029
6.467 ± 0.066
5.856 ± 0.048
5.414 ± 0.048
8.367 ± 0.057
1.058 ± 0.023
2.622 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here