Parolsenella catena 
Average proteome isoelectric point is 5.69 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 1584 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A3G9JXY2|A0A3G9JXY2_9ACTN RNA polymerase sigma-37 factor OS=Parolsenella catena OX=2003188 GN=sigB PE=4 SV=1 
MM1 pKa = 7.95  DD2 pKa = 6.32  PIIISVDD9 pKa = 3.03  EE10 pKa = 4.25  RR11 pKa = 11.84  LAGAGSVLPVAGHH24 pKa = 7.01  IDD26 pKa = 3.78  DD27 pKa = 5.53  DD28 pKa = 4.84  SYY30 pKa = 12.17  SVGDD34 pKa = 3.68  HH35 pKa = 7.2  DD36 pKa = 4.84  FRR38 pKa = 11.84  LPGGIDD44 pKa = 3.49  YY45 pKa = 11.23  DD46 pKa = 4.54  LVLTNAGEE54 pKa = 4.76  GILASGIVRR63 pKa = 11.84  AHH65 pKa = 6.11  VLGTCDD71 pKa = 3.37  RR72 pKa = 11.84  CLEE75 pKa = 4.22  DD76 pKa = 4.85  AEE78 pKa = 4.46  FDD80 pKa = 3.38  IASEE84 pKa = 3.87  VDD86 pKa = 3.47  EE87 pKa = 4.7  YY88 pKa = 11.74  FLFEE92 pKa = 4.55  APAEE96 pKa = 4.13  EE97 pKa = 4.98  DD98 pKa = 3.61  LSDD101 pKa = 5.94  DD102 pKa = 3.64  EE103 pKa = 6.06  DD104 pKa = 3.85  EE105 pKa = 4.7  VDD107 pKa = 4.43  FSLVGEE113 pKa = 4.4  DD114 pKa = 3.35  HH115 pKa = 6.96  TIDD118 pKa = 3.4  LAEE121 pKa = 5.5  PIMAAIVMEE130 pKa = 4.39  TPFVVLCRR138 pKa = 11.84  EE139 pKa = 4.22  DD140 pKa = 4.47  CKK142 pKa = 11.13  GLCPHH147 pKa = 7.15  CGANLNEE154 pKa = 4.51  GDD156 pKa = 4.28  CGCAEE161 pKa = 4.21  KK162 pKa = 10.08  MASVDD167 pKa = 3.25  AATGPFAALAGLRR180 pKa = 11.84  LDD182 pKa = 5.36  GEE184 pKa = 5.23  DD185 pKa = 4.04  GDD187 pKa = 4.63  SAQDD191 pKa = 3.19   
 Molecular weight: 20.14 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.718 
IPC2_protein 3.643 
IPC_protein 3.668 
Toseland    3.439 
ProMoST     3.808 
Dawson      3.656 
Bjellqvist  3.834 
Wikipedia   3.592 
Rodwell     3.49 
Grimsley    3.35 
Solomon     3.656 
Lehninger   3.605 
Nozaki      3.77 
DTASelect   4.012 
Thurlkill   3.503 
EMBOSS      3.605 
Sillero     3.783 
Patrickios  0.846 
IPC_peptide 3.643 
IPC2_peptide  3.757 
IPC2.peptide.svr19  3.725 
 Protein with the highest isoelectric point: 
>tr|A0A3G9K9Q8|A0A3G9K9Q8_9ACTN Molybdate ABC transporter substrate-binding protein OS=Parolsenella catena OX=2003188 GN=modA PE=3 SV=1 
MM1 pKa = 7.19  TARR4 pKa = 11.84  FTVIVIAKK12 pKa = 7.22  MWNSILSLSFMGAAPFRR29 pKa = 11.84  PPRR32 pKa = 11.84  QAASVRR38 pKa = 11.84  RR39 pKa = 11.84  FLAIRR44 pKa = 11.84  GAAKK48 pKa = 10.43  SLFGFHH54 pKa = 7.22  AARR57 pKa = 11.84  GRR59 pKa = 11.84  LPRR62 pKa = 11.84  SRR64 pKa = 11.84  APLASPDD71 pKa = 3.63  GGPFLASPVPLAATAGRR88 pKa = 11.84  PRR90 pKa = 11.84  PP91 pKa = 3.68   
 Molecular weight: 9.68 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.436 
IPC2_protein 10.935 
IPC_protein 12.544 
Toseland    12.705 
ProMoST     13.203 
Dawson      12.705 
Bjellqvist  12.705 
Wikipedia   13.173 
Rodwell     12.281 
Grimsley    12.749 
Solomon     13.203 
Lehninger   13.1 
Nozaki      12.705 
DTASelect   12.705 
Thurlkill   12.705 
EMBOSS      13.203 
Sillero     12.705 
Patrickios  12.018 
IPC_peptide 13.203 
IPC2_peptide  12.193 
IPC2.peptide.svr19  9.126 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        1584 
 
        
        0
 
        
        1584 
         
        527162
 
        42
 
        2467
 
        332.8
 
        36.02
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        11.944 ± 0.086
1.622 ± 0.027
 
        6.356 ± 0.047
6.689 ± 0.053
 
        3.266 ± 0.034
8.615 ± 0.059
 
        2.023 ± 0.029
4.608 ± 0.049
       
        3.445 ± 0.048
9.232 ± 0.067
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.593 ± 0.028
2.743 ± 0.037
 
        4.331 ± 0.04
2.749 ± 0.029
 
        6.467 ± 0.066
5.856 ± 0.048
 
        5.414 ± 0.048
8.367 ± 0.057
       
        1.058 ± 0.023
2.622 ± 0.031
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here