Octopus vulgaris (Common octopus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda; Coleoidea; Octopodiformes; Octopoda; Incirrata; Octopodidae; Octopus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7E6F7M3|A0A7E6F7M3_OCTVU Isoform of A0A7E6F7L6 Non-specific serine/threonine protein kinase OS=Octopus vulgaris OX=6645 GN=LOC115217321 PE=3 SV=1
MM1 pKa = 7.39LQQIMSFLAIGEE13 pKa = 4.1AAKK16 pKa = 10.41YY17 pKa = 10.6VPGKK21 pKa = 9.75PKK23 pKa = 10.55LPPYY27 pKa = 10.3GQDD30 pKa = 3.25KK31 pKa = 11.13LLMAVPDD38 pKa = 4.08KK39 pKa = 10.57PCRR42 pKa = 11.84SSKK45 pKa = 10.57IEE47 pKa = 3.73WFLFTDD53 pKa = 4.28ADD55 pKa = 4.13NLGSWLEE62 pKa = 4.26AIKK65 pKa = 8.2TTLPQQPDD73 pKa = 3.51SSNGTPLCTEE83 pKa = 4.3TSVNMLPQPPFSTPPLQPLPPPPPPPGGPYY113 pKa = 10.23YY114 pKa = 10.78LPNTFGTNINRR125 pKa = 11.84APPSYY130 pKa = 10.32PPMYY134 pKa = 8.76QQQVTTISPVGYY146 pKa = 9.14PRR148 pKa = 11.84IQQPPPQTVIVRR160 pKa = 11.84EE161 pKa = 4.12EE162 pKa = 3.9RR163 pKa = 11.84SSNDD167 pKa = 2.41GFAPGLLLGDD177 pKa = 3.83ALGFGLGAGWFSGGGYY193 pKa = 10.2GGGYY197 pKa = 8.65TGFDD201 pKa = 3.66DD202 pKa = 4.88SQNIQINNYY211 pKa = 9.65YY212 pKa = 10.73DD213 pKa = 3.45VDD215 pKa = 3.62NTNIEE220 pKa = 4.23NTDD223 pKa = 3.8FTDD226 pKa = 2.99VDD228 pKa = 3.63NSFDD232 pKa = 3.89TNNFDD237 pKa = 4.6IGNNDD242 pKa = 3.45GFCDD246 pKa = 4.54DD247 pKa = 4.69GFNFF251 pKa = 4.53

Molecular weight: 27.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P7SRX2|A0A6P7SRX2_OCTVU ubiquinol-cytochrome-c reductase complex assembly factor 1 isoform X1 OS=Octopus vulgaris OX=6645 GN=LOC115215843 PE=3 SV=1
MM1 pKa = 8.05PYY3 pKa = 10.02FADD6 pKa = 3.68EE7 pKa = 4.13VRR9 pKa = 11.84IKK11 pKa = 10.65QNSPIRR17 pKa = 11.84RR18 pKa = 11.84SSTTRR23 pKa = 11.84RR24 pKa = 11.84SSTTRR29 pKa = 11.84RR30 pKa = 11.84SSTILRR36 pKa = 11.84SSPTRR41 pKa = 11.84RR42 pKa = 11.84SSPTRR47 pKa = 11.84RR48 pKa = 11.84SSPTLRR54 pKa = 11.84SSPTRR59 pKa = 11.84RR60 pKa = 11.84SSPTRR65 pKa = 11.84RR66 pKa = 11.84SSTTRR71 pKa = 11.84RR72 pKa = 11.84SSLARR77 pKa = 11.84RR78 pKa = 11.84NSPIRR83 pKa = 11.84RR84 pKa = 11.84NSPIRR89 pKa = 11.84RR90 pKa = 11.84NSPTRR95 pKa = 11.84RR96 pKa = 11.84SSPTLRR102 pKa = 11.84NSPTLRR108 pKa = 11.84SSPIRR113 pKa = 11.84RR114 pKa = 11.84SSPIRR119 pKa = 11.84RR120 pKa = 11.84NSPIRR125 pKa = 11.84RR126 pKa = 11.84NSPTRR131 pKa = 11.84RR132 pKa = 11.84SSTTLRR138 pKa = 11.84NSPIRR143 pKa = 11.84RR144 pKa = 11.84SSPIRR149 pKa = 11.84RR150 pKa = 11.84SSPTRR155 pKa = 11.84RR156 pKa = 11.84SSPTRR161 pKa = 11.84RR162 pKa = 11.84SSPTRR167 pKa = 11.84RR168 pKa = 11.84RR169 pKa = 11.84SPTRR173 pKa = 11.84RR174 pKa = 11.84SSSFII179 pKa = 3.76

Molecular weight: 20.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18617

11713

30330

20536984

9

18301

677.1

76.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.312 ± 0.013

2.245 ± 0.016

5.561 ± 0.016

6.734 ± 0.025

3.718 ± 0.011

5.148 ± 0.021

2.74 ± 0.013

5.555 ± 0.013

7.197 ± 0.018

8.47 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.007

5.433 ± 0.017

5.05 ± 0.019

4.558 ± 0.016

4.637 ± 0.014

9.423 ± 0.028

6.351 ± 0.03

5.765 ± 0.012

0.908 ± 0.005

2.995 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski