Firmicutes bacterium CAG:460

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7IED8|R7IED8_9FIRM Surface antigen BspA-like OS=Firmicutes bacterium CAG:460 OX=1263024 GN=BN665_00720 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.34KK3 pKa = 10.74LNVNEE8 pKa = 3.94NCIGCGMCAAIDD20 pKa = 3.93PEE22 pKa = 4.17HH23 pKa = 7.23FEE25 pKa = 4.24IVDD28 pKa = 3.83GLSQATNSDD37 pKa = 3.66NLDD40 pKa = 3.66SKK42 pKa = 11.34DD43 pKa = 3.75LEE45 pKa = 4.44NAISSCPTAAIEE57 pKa = 5.25IIEE60 pKa = 4.2EE61 pKa = 4.25EE62 pKa = 4.18NN63 pKa = 3.12

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7I9J2|R7I9J2_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:460 OX=1263024 GN=BN665_00141 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.4KK4 pKa = 10.86SMIVKK9 pKa = 9.4NQRR12 pKa = 11.84APKK15 pKa = 9.73FSTRR19 pKa = 11.84AYY21 pKa = 8.32TRR23 pKa = 11.84CNRR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 6.61GVLRR35 pKa = 11.84KK36 pKa = 10.07YY37 pKa = 9.84GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.85LANEE50 pKa = 4.18GKK52 pKa = 10.74LPGVKK57 pKa = 9.87KK58 pKa = 10.9SSWW61 pKa = 3.1

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1169

0

1169

325602

29

1437

278.5

31.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.592 ± 0.072

1.08 ± 0.028

6.123 ± 0.055

7.34 ± 0.083

4.423 ± 0.057

5.413 ± 0.074

1.245 ± 0.028

9.824 ± 0.092

10.019 ± 0.077

9.176 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.031

7.607 ± 0.071

2.369 ± 0.038

1.779 ± 0.031

2.97 ± 0.048

6.09 ± 0.069

5.266 ± 0.066

6.462 ± 0.066

0.608 ± 0.02

5.18 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski