Streptomyces indicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7387 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9DYZ9|A0A1G9DYZ9_9ACTN SsrA-binding protein OS=Streptomyces indicus OX=417292 GN=smpB PE=3 SV=1
MM1 pKa = 7.59SGTDD5 pKa = 3.56GNGVPGYY12 pKa = 10.36GAGDD16 pKa = 4.36GYY18 pKa = 11.48GSGGHH23 pKa = 5.58VSNGQTGEE31 pKa = 3.78GSYY34 pKa = 11.72GDD36 pKa = 4.04GTGGPYY42 pKa = 10.68GDD44 pKa = 4.25GTGGSYY50 pKa = 11.24GDD52 pKa = 4.01GAGAPYY58 pKa = 10.68EE59 pKa = 4.33NGGGAAYY66 pKa = 9.96GHH68 pKa = 6.78GGWQPTSQGDD78 pKa = 3.76YY79 pKa = 11.1DD80 pKa = 4.09GDD82 pKa = 3.52ATAFVALPEE91 pKa = 4.43GLADD95 pKa = 4.04TPLAAPGGNFVPPQISVVPGAAADD119 pKa = 3.78PAATGTFVIPSLVPQMGADD138 pKa = 3.47SAHH141 pKa = 6.41AQPASSPVPSAPAPAPAPVQWPEE164 pKa = 3.88PNAPQQPAPPHH175 pKa = 5.3EE176 pKa = 5.58AGMTGQWSFTEE187 pKa = 4.31AAAQAGPAGQPEE199 pKa = 4.6PVPDD203 pKa = 3.93GQAAQASHH211 pKa = 6.7SPQGNGPHH219 pKa = 7.06ASGPHH224 pKa = 5.85ANVTGQWSIPLAQGDD239 pKa = 4.33LPDD242 pKa = 3.83EE243 pKa = 4.68SGEE246 pKa = 4.29FTSSLADD253 pKa = 3.18QWADD257 pKa = 3.7RR258 pKa = 11.84APATLPGGAPAPWAHH273 pKa = 7.05LVPQDD278 pKa = 4.11EE279 pKa = 4.53EE280 pKa = 4.37QAGQPAQTPHH290 pKa = 6.95GAQEE294 pKa = 3.93PQPAQVGPAEE304 pKa = 4.21AAAAGPEE311 pKa = 4.09PVAAQPEE318 pKa = 4.59DD319 pKa = 3.58PAVPDD324 pKa = 3.85QEE326 pKa = 4.33PQAAEE331 pKa = 4.06APEE334 pKa = 4.06QTDD337 pKa = 3.07QTEE340 pKa = 4.14QTDD343 pKa = 3.54QTEE346 pKa = 4.16QTEE349 pKa = 4.57PARR352 pKa = 11.84QADD355 pKa = 3.71PADD358 pKa = 4.26RR359 pKa = 11.84PEE361 pKa = 4.38QPDD364 pKa = 3.35EE365 pKa = 4.07TAEE368 pKa = 4.07SAEE371 pKa = 4.09AGRR374 pKa = 11.84DD375 pKa = 3.75TEE377 pKa = 4.06EE378 pKa = 3.97SAAFFADD385 pKa = 3.66EE386 pKa = 5.0HH387 pKa = 6.56PLGSYY392 pKa = 7.74VLRR395 pKa = 11.84VNGTDD400 pKa = 4.45RR401 pKa = 11.84PVTDD405 pKa = 2.98AWIGEE410 pKa = 4.15SLLYY414 pKa = 10.18VLRR417 pKa = 11.84EE418 pKa = 3.94RR419 pKa = 11.84LGLAGAKK426 pKa = 9.72DD427 pKa = 3.75GCSQGEE433 pKa = 4.32CGACAVQVDD442 pKa = 3.9GRR444 pKa = 11.84LVASCLVPAATAAGSEE460 pKa = 4.08VRR462 pKa = 11.84TVEE465 pKa = 4.06GLAADD470 pKa = 4.65GQPSDD475 pKa = 3.76VQRR478 pKa = 11.84ALAASCAVQCGFCVPGMAMTVHH500 pKa = 7.27DD501 pKa = 5.21LLEE504 pKa = 4.93GNPQPTEE511 pKa = 3.98RR512 pKa = 11.84EE513 pKa = 3.93TRR515 pKa = 11.84QALCGNLCRR524 pKa = 11.84CSGYY528 pKa = 10.62KK529 pKa = 10.22GVLEE533 pKa = 4.01AVKK536 pKa = 10.63DD537 pKa = 4.19VVAEE541 pKa = 4.12RR542 pKa = 11.84QAAVPDD548 pKa = 3.75EE549 pKa = 5.09DD550 pKa = 4.1DD551 pKa = 3.82PARR554 pKa = 11.84IPHH557 pKa = 5.69QAPPGAGGVNYY568 pKa = 9.37PQDD571 pKa = 3.39GGRR574 pKa = 11.84AA575 pKa = 3.49

Molecular weight: 57.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9FBE4|A0A1G9FBE4_9ACTN Acetoacetate decarboxylase OS=Streptomyces indicus OX=417292 GN=SAMN05421806_11324 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.74GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7387

0

7387

2409369

28

2576

326.2

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.777 ± 0.045

0.776 ± 0.009

5.817 ± 0.02

5.984 ± 0.03

2.765 ± 0.016

9.551 ± 0.027

2.221 ± 0.016

3.24 ± 0.02

2.512 ± 0.024

10.313 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.75 ± 0.013

1.744 ± 0.017

6.081 ± 0.025

2.895 ± 0.016

7.748 ± 0.037

4.974 ± 0.023

5.996 ± 0.03

8.236 ± 0.025

1.516 ± 0.012

2.104 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski