Beihai blue swimmer crab virus 3
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFC4|A0A1L3KFC4_9VIRU Uncharacterized protein OS=Beihai blue swimmer crab virus 3 OX=1922370 PE=4 SV=1
MM1 pKa = 7.01 SHH3 pKa = 6.61 TYY5 pKa = 10.27 SVEE8 pKa = 4.0 HH9 pKa = 6.7 EE10 pKa = 4.61 SKK12 pKa = 10.97 CGGKK16 pKa = 10.36 LYY18 pKa = 10.39 TGTVDD23 pKa = 3.48 GSVEE27 pKa = 3.94 FSVVGLGKK35 pKa = 10.69 GDD37 pKa = 4.53 FSMQLQTRR45 pKa = 11.84 PSEE48 pKa = 3.95 EE49 pKa = 3.88 DD50 pKa = 3.21 LKK52 pKa = 11.34 RR53 pKa = 11.84 EE54 pKa = 3.79 AARR57 pKa = 11.84 RR58 pKa = 11.84 EE59 pKa = 3.83 LVKK62 pKa = 10.58 QKK64 pKa = 10.77 KK65 pKa = 8.22 EE66 pKa = 3.94 KK67 pKa = 10.19 KK68 pKa = 8.91 DD69 pKa = 3.59 NKK71 pKa = 7.71 STRR74 pKa = 11.84 VEE76 pKa = 4.21 KK77 pKa = 10.71 LGHH80 pKa = 5.87 ALKK83 pKa = 10.33 HH84 pKa = 5.75 LSVSKK89 pKa = 10.55 QDD91 pKa = 3.89 EE92 pKa = 4.41 NSSSSSLPHH101 pKa = 5.53 MRR103 pKa = 11.84 AVMSEE108 pKa = 4.14 LTPLEE113 pKa = 4.14 PSLVQVCKK121 pKa = 10.38 VSQHH125 pKa = 5.74 RR126 pKa = 11.84 FMGFLDD132 pKa = 3.92 GLNAEE137 pKa = 5.43 LIDD140 pKa = 4.2 SSGAFNSDD148 pKa = 3.27 LLGEE152 pKa = 4.33 QLRR155 pKa = 11.84 KK156 pKa = 10.15 LPGYY160 pKa = 9.74 NKK162 pKa = 9.51 RR163 pKa = 11.84 DD164 pKa = 3.69 YY165 pKa = 11.66 DD166 pKa = 5.34 DD167 pKa = 3.62 MQVWLAAVDD176 pKa = 3.61 KK177 pKa = 10.95 CQGEE181 pKa = 4.17 SHH183 pKa = 6.88 ARR185 pKa = 11.84 LLYY188 pKa = 11.24 NMACSITRR196 pKa = 11.84 AQLVQNSTLYY206 pKa = 10.2 NSEE209 pKa = 3.87 RR210 pKa = 11.84 RR211 pKa = 11.84 TGSVLGYY218 pKa = 7.44 THH220 pKa = 7.38 FDD222 pKa = 2.87 SHH224 pKa = 6.8 TISSQYY230 pKa = 9.58 EE231 pKa = 3.99 HH232 pKa = 7.13 GPGLVEE238 pKa = 4.02 VNMYY242 pKa = 11.21 ADD244 pKa = 3.81 VSRR247 pKa = 11.84 PATVTVVDD255 pKa = 3.73 RR256 pKa = 11.84 DD257 pKa = 3.81 SFRR260 pKa = 11.84 GEE262 pKa = 4.36 HH263 pKa = 6.18 VSHH266 pKa = 6.52 LHH268 pKa = 6.41 IPNKK272 pKa = 7.2 TAKK275 pKa = 10.28 QIAFVLGHH283 pKa = 5.47 SGKK286 pKa = 10.36 RR287 pKa = 11.84 EE288 pKa = 4.05 VKK290 pKa = 10.22 DD291 pKa = 3.18 SAFNFEE297 pKa = 5.24 FGIPDD302 pKa = 4.06 LGVKK306 pKa = 9.45 NVSYY310 pKa = 10.04 TDD312 pKa = 3.38 KK313 pKa = 11.36 VEE315 pKa = 4.28 PDD317 pKa = 2.98 QVLDD321 pKa = 3.72 YY322 pKa = 10.44 FVQDD326 pKa = 3.5 TLALDD331 pKa = 3.79 LSRR334 pKa = 11.84 YY335 pKa = 9.96 SIAEE339 pKa = 3.76 MTAALQVYY347 pKa = 8.44 IKK349 pKa = 10.7 SMRR352 pKa = 11.84 LEE354 pKa = 3.93 AEE356 pKa = 4.09 YY357 pKa = 10.93 LVVCEE362 pKa = 4.24 VMNLSGFFPLPDD374 pKa = 3.56 TRR376 pKa = 11.84 EE377 pKa = 4.27 GTWWSRR383 pKa = 11.84 FRR385 pKa = 11.84 VQVCLPEE392 pKa = 4.28 FFSLRR397 pKa = 11.84 SAHH400 pKa = 6.16 PMMVEE405 pKa = 4.09 GSPYY409 pKa = 9.51 TIEE412 pKa = 5.07 KK413 pKa = 9.95 IGAHH417 pKa = 5.06 TTSISQQNSYY427 pKa = 10.86 RR428 pKa = 11.84 SMIDD432 pKa = 3.3 YY433 pKa = 11.08 AVMNYY438 pKa = 10.31 RR439 pKa = 11.84 IMLGVYY445 pKa = 8.6 EE446 pKa = 4.23 KK447 pKa = 10.73 VRR449 pKa = 11.84 RR450 pKa = 11.84 VAEE453 pKa = 5.08 GIDD456 pKa = 3.55 SFGPDD461 pKa = 3.15 SAAHH465 pKa = 5.96 DD466 pKa = 3.79 VPYY469 pKa = 10.47 EE470 pKa = 3.89 YY471 pKa = 11.13 LSVFEE476 pKa = 4.23 STEE479 pKa = 4.11 LEE481 pKa = 4.24 SGLVPGYY488 pKa = 9.96 GVNYY492 pKa = 9.44 NAEE495 pKa = 4.33 EE496 pKa = 4.15 IARR499 pKa = 11.84 AYY501 pKa = 10.8 ADD503 pKa = 3.75 GDD505 pKa = 3.63 VKK507 pKa = 11.05 YY508 pKa = 10.39 YY509 pKa = 10.8 YY510 pKa = 10.82 YY511 pKa = 10.16 EE512 pKa = 4.34 TDD514 pKa = 3.45 PDD516 pKa = 3.73 SYY518 pKa = 10.89 AAHH521 pKa = 6.33 MKK523 pKa = 11.02 DD524 pKa = 3.43 NDD526 pKa = 3.55 VLPAHH531 pKa = 6.97 KK532 pKa = 10.92 GEE534 pKa = 4.25 LCAGNTTSVPPPTSLVPLYY553 pKa = 9.5 GACPAEE559 pKa = 3.95 LLAYY563 pKa = 10.2 QYY565 pKa = 10.61 MNGGRR570 pKa = 11.84 IEE572 pKa = 5.58 AFDD575 pKa = 3.66 QSKK578 pKa = 10.2 GAAEE582 pKa = 3.97 YY583 pKa = 10.36 FSYY586 pKa = 11.18 QEE588 pKa = 3.99 MMLVANFLRR597 pKa = 11.84 LNRR600 pKa = 11.84 TDD602 pKa = 3.6 STWQEE607 pKa = 3.96 VQRR610 pKa = 11.84 GCDD613 pKa = 3.21 TEE615 pKa = 4.63 VFTGAMSKK623 pKa = 8.53 VTHH626 pKa = 6.45 APFYY630 pKa = 10.64 YY631 pKa = 9.96 DD632 pKa = 4.83 DD633 pKa = 5.11 PMVQYY638 pKa = 11.22 LNTSTRR644 pKa = 11.84 KK645 pKa = 9.33 RR646 pKa = 11.84 DD647 pKa = 3.59 PEE649 pKa = 4.12 SRR651 pKa = 11.84 SCGPGYY657 pKa = 10.56 QSICTYY663 pKa = 10.14 GLQVAYY669 pKa = 10.03 RR670 pKa = 11.84 ALWSGVKK677 pKa = 9.64 SYY679 pKa = 11.3 RR680 pKa = 11.84 RR681 pKa = 11.84 FTVSYY686 pKa = 9.8 RR687 pKa = 11.84 GVRR690 pKa = 11.84 ANRR693 pKa = 11.84 RR694 pKa = 11.84 SRR696 pKa = 11.84 PLEE699 pKa = 3.8 FKK701 pKa = 10.57 VAGSATYY708 pKa = 10.21 HH709 pKa = 6.37 KK710 pKa = 10.21 FVGGAPLTSPEE721 pKa = 3.86 QDD723 pKa = 3.15 HH724 pKa = 6.76 LGEE727 pKa = 4.76 SNFHH731 pKa = 6.39 KK732 pKa = 10.37 VQKK735 pKa = 9.97 AAPAVGPSTIVHH747 pKa = 5.54 NTASTQDD754 pKa = 3.16 TGDD757 pKa = 3.13 QHH759 pKa = 7.42 AAEE762 pKa = 4.89 ISQDD766 pKa = 3.81 LPEE769 pKa = 4.07 QTPPVEE775 pKa = 4.24 EE776 pKa = 4.74 GGSGKK781 pKa = 8.57 QQ782 pKa = 3.14
Molecular weight: 87.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.555
IPC2_protein 5.55
IPC_protein 5.601
Toseland 5.995
ProMoST 5.919
Dawson 5.83
Bjellqvist 5.855
Wikipedia 5.817
Rodwell 5.804
Grimsley 6.16
Solomon 5.83
Lehninger 5.817
Nozaki 6.084
DTASelect 6.262
Thurlkill 6.262
EMBOSS 6.211
Sillero 6.173
Patrickios 3.795
IPC_peptide 5.842
IPC2_peptide 6.186
IPC2.peptide.svr19 6.105
Protein with the highest isoelectric point:
>tr|A0A1L3KFC4|A0A1L3KFC4_9VIRU Uncharacterized protein OS=Beihai blue swimmer crab virus 3 OX=1922370 PE=4 SV=1
MM1 pKa = 6.9 MAVTGTIEE9 pKa = 4.04 YY10 pKa = 9.75 VEE12 pKa = 5.0 KK13 pKa = 10.46 YY14 pKa = 9.61 LHH16 pKa = 6.12 YY17 pKa = 9.46 NQIVAGDD24 pKa = 3.89 CSGMFLATLEE34 pKa = 4.01 YY35 pKa = 10.07 EE36 pKa = 4.27 RR37 pKa = 11.84 RR38 pKa = 11.84 VLSALVLRR46 pKa = 11.84 NVHH49 pKa = 6.06 TNTASLYY56 pKa = 10.48 LRR58 pKa = 11.84 ADD60 pKa = 3.86 YY61 pKa = 10.58 KK62 pKa = 11.28 YY63 pKa = 9.83 GAYY66 pKa = 9.12 WPFFKK71 pKa = 10.6 QVVSTNMCVNTSRR84 pKa = 11.84 AAVYY88 pKa = 10.04 GVDD91 pKa = 3.36 YY92 pKa = 10.61 SAPFRR97 pKa = 11.84 HH98 pKa = 6.15 LASSSKK104 pKa = 10.32 VKK106 pKa = 10.31 QSRR109 pKa = 11.84 KK110 pKa = 10.38 DD111 pKa = 3.41 IILLTTSEE119 pKa = 3.9 QVAYY123 pKa = 10.82 LEE125 pKa = 4.12 EE126 pKa = 4.31 LPRR129 pKa = 11.84 AKK131 pKa = 10.02 ISADD135 pKa = 3.15 HH136 pKa = 6.95 HH137 pKa = 5.85 IHH139 pKa = 6.44 YY140 pKa = 10.44 SAIDD144 pKa = 3.14 SWNVLKK150 pKa = 10.97 EE151 pKa = 3.76 KK152 pKa = 10.87 DD153 pKa = 3.37 RR154 pKa = 11.84 DD155 pKa = 3.63 LATSLVSLLSRR166 pKa = 11.84 QKK168 pKa = 9.35 GWTMTGISGVLGYY181 pKa = 10.66 AALLGKK187 pKa = 10.38 CCAFRR192 pKa = 11.84 LHH194 pKa = 6.59 SRR196 pKa = 11.84 DD197 pKa = 3.1 FFTPGSSMNEE207 pKa = 3.16 WYY209 pKa = 10.34 GFHH212 pKa = 7.45 KK213 pKa = 10.3 ATSTNYY219 pKa = 10.17 KK220 pKa = 7.89 MHH222 pKa = 6.93 ANIMNYY228 pKa = 9.56 RR229 pKa = 11.84 LEE231 pKa = 3.95 EE232 pKa = 4.36 LYY234 pKa = 10.7 EE235 pKa = 4.14 VEE237 pKa = 4.3 VLVNRR242 pKa = 11.84 LDD244 pKa = 5.79 LGMDD248 pKa = 3.07 WDD250 pKa = 3.82 KK251 pKa = 11.44 EE252 pKa = 4.47 YY253 pKa = 11.69 VNRR256 pKa = 11.84 TTMNLTRR263 pKa = 11.84 LQDD266 pKa = 3.54 AQVYY270 pKa = 9.12 KK271 pKa = 10.45 AASWLFRR278 pKa = 11.84 TARR281 pKa = 11.84 ASGKK285 pKa = 10.12 RR286 pKa = 11.84 PNKK289 pKa = 9.5 QNWNKK294 pKa = 9.24 FWSKK298 pKa = 8.87 RR299 pKa = 11.84 WEE301 pKa = 3.93 WSTTGATNYY310 pKa = 8.43 PYY312 pKa = 10.44 PNDD315 pKa = 3.47 FQYY318 pKa = 10.58 PAWYY322 pKa = 9.98 AKK324 pKa = 10.35 NKK326 pKa = 9.57 FMTYY330 pKa = 9.86 LQMRR334 pKa = 11.84 SGLEE338 pKa = 4.01 LNEE341 pKa = 4.01 LAAMPARR348 pKa = 11.84 ITAKK352 pKa = 10.41 PSIKK356 pKa = 10.57 YY357 pKa = 9.06 EE358 pKa = 3.8 WGKK361 pKa = 8.07 TRR363 pKa = 11.84 AIYY366 pKa = 10.46 GCDD369 pKa = 3.97 DD370 pKa = 3.43 ISHH373 pKa = 6.9 LVYY376 pKa = 10.52 TFVLGSPEE384 pKa = 4.1 EE385 pKa = 4.55 LLPSWIPLGASSKK398 pKa = 11.1 DD399 pKa = 3.53 EE400 pKa = 4.11 YY401 pKa = 11.38 VHH403 pKa = 6.67 NLLNQVSSKK412 pKa = 10.82 GYY414 pKa = 8.83 SLCLDD419 pKa = 3.74 FEE421 pKa = 5.24 DD422 pKa = 5.84 FNSQHH427 pKa = 6.48 SSGNMRR433 pKa = 11.84 AVLEE437 pKa = 4.28 AFFDD441 pKa = 4.23 VYY443 pKa = 11.34 SQDD446 pKa = 3.78 LTDD449 pKa = 4.61 DD450 pKa = 3.61 QLKK453 pKa = 9.82 LRR455 pKa = 11.84 RR456 pKa = 11.84 WVLDD460 pKa = 3.42 SVLNQYY466 pKa = 9.54 VMPGSGNTHH475 pKa = 7.29 AYY477 pKa = 7.75 RR478 pKa = 11.84 TEE480 pKa = 3.89 GTLFSGWRR488 pKa = 11.84 LTTFMNTVMNYY499 pKa = 10.12 CYY501 pKa = 8.88 TQCLVEE507 pKa = 4.9 DD508 pKa = 4.56 SGAKK512 pKa = 10.04 SYY514 pKa = 8.47 GTLHH518 pKa = 6.69 SGDD521 pKa = 4.18 DD522 pKa = 3.57 VFMSVMEE529 pKa = 4.47 PNDD532 pKa = 4.03 YY533 pKa = 11.0 IRR535 pKa = 11.84 MQFSAEE541 pKa = 3.46 RR542 pKa = 11.84 WNIRR546 pKa = 11.84 LSLQKK551 pKa = 10.85 CFMTSIKK558 pKa = 10.5 EE559 pKa = 4.13 FLRR562 pKa = 11.84 KK563 pKa = 9.49 DD564 pKa = 3.6 HH565 pKa = 5.2 QHH567 pKa = 6.44 KK568 pKa = 10.89 AGAQYY573 pKa = 10.76 LARR576 pKa = 11.84 GVSTLVHH583 pKa = 6.1 GRR585 pKa = 11.84 TEE587 pKa = 4.52 SVQHH591 pKa = 6.2 SDD593 pKa = 3.96 IIEE596 pKa = 4.01 LLKK599 pKa = 11.2 ANEE602 pKa = 4.01 TRR604 pKa = 11.84 LEE606 pKa = 4.23 EE607 pKa = 3.95 IVNRR611 pKa = 11.84 EE612 pKa = 3.85 GSPKK616 pKa = 10.3 VVDD619 pKa = 4.0 HH620 pKa = 6.26 FRR622 pKa = 11.84 KK623 pKa = 9.93 IYY625 pKa = 9.43 LARR628 pKa = 11.84 IKK630 pKa = 10.28 RR631 pKa = 11.84 DD632 pKa = 3.78 PIYY635 pKa = 10.78 EE636 pKa = 4.78 GITDD640 pKa = 3.59 EE641 pKa = 4.81 EE642 pKa = 3.76 IRR644 pKa = 11.84 AIYY647 pKa = 9.07 EE648 pKa = 3.53 LHH650 pKa = 6.51 PVNKK654 pKa = 8.82 GMNMAAEE661 pKa = 4.2 AKK663 pKa = 10.37 GYY665 pKa = 10.15 GLQLVSRR672 pKa = 11.84 RR673 pKa = 11.84 KK674 pKa = 8.39 HH675 pKa = 4.3 TLEE678 pKa = 5.32 GYY680 pKa = 9.84 DD681 pKa = 3.52 LPGVRR686 pKa = 11.84 DD687 pKa = 3.67 YY688 pKa = 11.76 VHH690 pKa = 6.79 EE691 pKa = 4.29 LKK693 pKa = 10.64 KK694 pKa = 10.63 YY695 pKa = 10.78 LKK697 pKa = 9.08 DD698 pKa = 3.47 TKK700 pKa = 10.4 HH701 pKa = 5.67 VKK703 pKa = 9.46 QLSRR707 pKa = 11.84 SIWNATEE714 pKa = 3.92 RR715 pKa = 11.84 VIFSTVTRR723 pKa = 11.84 VSGFIEE729 pKa = 3.7 MDD731 pKa = 3.34 LAEE734 pKa = 4.28 RR735 pKa = 11.84 RR736 pKa = 11.84 VMKK739 pKa = 10.29 SLYY742 pKa = 10.15 KK743 pKa = 10.55 LYY745 pKa = 10.84 SRR747 pKa = 11.84 YY748 pKa = 9.88 KK749 pKa = 10.62 ADD751 pKa = 2.67 IHH753 pKa = 6.56 YY754 pKa = 8.67 GKK756 pKa = 10.27 CRR758 pKa = 11.84 LVGMDD763 pKa = 3.34 VVSRR767 pKa = 11.84 DD768 pKa = 3.17 LSEE771 pKa = 4.83 SIRR774 pKa = 11.84 HH775 pKa = 4.65 VDD777 pKa = 3.26 IVIGDD782 pKa = 3.66 KK783 pKa = 10.61 PNYY786 pKa = 9.31 YY787 pKa = 9.68 EE788 pKa = 4.24 WLSILII794 pKa = 4.35
Molecular weight: 91.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.149
IPC2_protein 8.258
IPC_protein 8.112
Toseland 8.375
ProMoST 8.58
Dawson 8.902
Bjellqvist 8.916
Wikipedia 9.048
Rodwell 8.975
Grimsley 8.77
Solomon 8.946
Lehninger 8.902
Nozaki 8.916
DTASelect 8.785
Thurlkill 8.829
EMBOSS 9.019
Sillero 9.033
Patrickios 4.151
IPC_peptide 8.931
IPC2_peptide 7.688
IPC2.peptide.svr19 7.726
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1576
782
794
788.0
89.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.853 ± 0.221
1.332 ± 0.053
5.457 ± 0.121
6.345 ± 0.401
3.426 ± 0.11
6.282 ± 0.629
3.173 ± 0.017
3.426 ± 0.713
5.52 ± 0.473
8.693 ± 0.73
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.046 ± 0.166
3.934 ± 0.436
3.617 ± 0.797
3.617 ± 0.523
6.028 ± 0.379
8.82 ± 0.46
5.584 ± 0.061
7.487 ± 0.498
1.459 ± 0.587
5.901 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here