Mycobacterium phage DarthPhader

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9S433|A0A1I9S433_9CAUD Uncharacterized protein OS=Mycobacterium phage DarthPhader OX=1912975 GN=SEA_DARTHPHADER_87 PE=4 SV=1
MM1 pKa = 7.77PNLTNVLLSLVAAGAALGAMALADD25 pKa = 4.27PYY27 pKa = 10.29EE28 pKa = 4.65ASAEE32 pKa = 4.09PEE34 pKa = 3.87EE35 pKa = 4.52TTTVEE40 pKa = 3.9VDD42 pKa = 3.4SVLLDD47 pKa = 3.46FPVCHH52 pKa = 7.39LEE54 pKa = 4.31DD55 pKa = 5.1CSDD58 pKa = 3.35QAEE61 pKa = 4.33QVGVWISNEE70 pKa = 4.04GISYY74 pKa = 9.66LIVGEE79 pKa = 4.14STTRR83 pKa = 11.84ITRR86 pKa = 3.46

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9S3Z8|A0A1I9S3Z8_9CAUD Metallophosphoesterase OS=Mycobacterium phage DarthPhader OX=1912975 GN=SEA_DARTHPHADER_52 PE=4 SV=1
MM1 pKa = 7.41TPTRR5 pKa = 11.84LPYY8 pKa = 9.66LHH10 pKa = 7.02KK11 pKa = 10.39NARR14 pKa = 11.84SRR16 pKa = 11.84QITSKK21 pKa = 10.54EE22 pKa = 3.58IRR24 pKa = 11.84EE25 pKa = 4.16VFADD29 pKa = 4.06EE30 pKa = 4.4VARR33 pKa = 11.84GFQPTRR39 pKa = 11.84QMSRR43 pKa = 11.84EE44 pKa = 3.72QYY46 pKa = 9.52LRR48 pKa = 11.84KK49 pKa = 10.23VMPP52 pKa = 4.49

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

16769

27

1037

184.3

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.221 ± 0.39

0.829 ± 0.118

6.059 ± 0.213

6.381 ± 0.303

3.345 ± 0.179

9.017 ± 0.65

1.867 ± 0.163

5.146 ± 0.155

4.58 ± 0.181

7.949 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.147

3.196 ± 0.144

5.337 ± 0.272

3.751 ± 0.222

6.262 ± 0.298

6.071 ± 0.261

5.945 ± 0.178

6.87 ± 0.195

1.878 ± 0.145

2.785 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski