Amino acid dipepetide frequency for Mycobacterium phage QuickMath

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.889AlaAla: 13.889 ± 1.546
1.148AlaCys: 1.148 ± 0.284
7.655AlaAsp: 7.655 ± 0.587
6.944AlaGlu: 6.944 ± 0.669
3.007AlaPhe: 3.007 ± 0.455
9.678AlaGly: 9.678 ± 1.115
2.515AlaHis: 2.515 ± 0.36
4.484AlaIle: 4.484 ± 0.611
4.703AlaLys: 4.703 ± 0.534
7.874AlaLeu: 7.874 ± 0.911
2.734AlaMet: 2.734 ± 0.43
2.461AlaAsn: 2.461 ± 0.366
5.031AlaPro: 5.031 ± 0.497
3.5AlaGln: 3.5 ± 0.402
8.093AlaArg: 8.093 ± 0.723
5.577AlaSer: 5.577 ± 0.571
7.218AlaThr: 7.218 ± 0.515
6.562AlaVal: 6.562 ± 0.701
2.461AlaTrp: 2.461 ± 0.396
2.023AlaTyr: 2.023 ± 0.329
0.0AlaXaa: 0.0 ± 0.0
Cys
0.82CysAla: 0.82 ± 0.235
0.0CysCys: 0.0 ± 0.0
1.422CysAsp: 1.422 ± 0.395
0.656CysGlu: 0.656 ± 0.188
0.164CysPhe: 0.164 ± 0.091
1.914CysGly: 1.914 ± 0.422
0.219CysHis: 0.219 ± 0.119
0.219CysIle: 0.219 ± 0.12
0.383CysLys: 0.383 ± 0.15
0.875CysLeu: 0.875 ± 0.25
0.164CysMet: 0.164 ± 0.106
0.437CysAsn: 0.437 ± 0.155
1.258CysPro: 1.258 ± 0.281
0.273CysGln: 0.273 ± 0.117
0.547CysArg: 0.547 ± 0.174
0.984CysSer: 0.984 ± 0.226
0.711CysThr: 0.711 ± 0.22
0.656CysVal: 0.656 ± 0.191
0.273CysTrp: 0.273 ± 0.12
0.164CysTyr: 0.164 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
7.108AspAla: 7.108 ± 0.662
0.93AspCys: 0.93 ± 0.202
4.538AspAsp: 4.538 ± 0.529
3.664AspGlu: 3.664 ± 0.565
1.914AspPhe: 1.914 ± 0.259
6.835AspGly: 6.835 ± 0.679
1.367AspHis: 1.367 ± 0.273
2.406AspIle: 2.406 ± 0.358
1.695AspLys: 1.695 ± 0.299
5.906AspLeu: 5.906 ± 0.557
0.93AspMet: 0.93 ± 0.271
1.476AspAsn: 1.476 ± 0.338
5.523AspPro: 5.523 ± 0.614
2.242AspGln: 2.242 ± 0.339
5.249AspArg: 5.249 ± 0.683
3.226AspSer: 3.226 ± 0.504
3.882AspThr: 3.882 ± 0.537
4.32AspVal: 4.32 ± 0.646
1.531AspTrp: 1.531 ± 0.295
1.914AspTyr: 1.914 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
6.452GluAla: 6.452 ± 0.659
0.82GluCys: 0.82 ± 0.226
2.625GluAsp: 2.625 ± 0.305
3.062GluGlu: 3.062 ± 0.55
2.078GluPhe: 2.078 ± 0.33
3.281GluGly: 3.281 ± 0.456
1.859GluHis: 1.859 ± 0.443
1.969GluIle: 1.969 ± 0.317
2.187GluLys: 2.187 ± 0.301
5.304GluLeu: 5.304 ± 0.622
1.75GluMet: 1.75 ± 0.324
1.859GluAsn: 1.859 ± 0.32
3.117GluPro: 3.117 ± 0.425
2.625GluGln: 2.625 ± 0.407
4.867GluArg: 4.867 ± 0.634
3.062GluSer: 3.062 ± 0.5
3.882GluThr: 3.882 ± 0.557
4.32GluVal: 4.32 ± 0.477
1.75GluTrp: 1.75 ± 0.276
1.804GluTyr: 1.804 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
2.953PheAla: 2.953 ± 0.396
0.437PheCys: 0.437 ± 0.148
2.351PheAsp: 2.351 ± 0.4
1.695PheGlu: 1.695 ± 0.32
1.094PhePhe: 1.094 ± 0.301
2.625PheGly: 2.625 ± 0.621
0.437PheHis: 0.437 ± 0.143
1.422PheIle: 1.422 ± 0.387
1.039PheLys: 1.039 ± 0.217
1.914PheLeu: 1.914 ± 0.278
0.656PheMet: 0.656 ± 0.221
1.094PheAsn: 1.094 ± 0.317
1.586PhePro: 1.586 ± 0.329
1.094PheGln: 1.094 ± 0.325
1.586PheArg: 1.586 ± 0.257
1.64PheSer: 1.64 ± 0.305
2.406PheThr: 2.406 ± 0.444
2.023PheVal: 2.023 ± 0.286
0.547PheTrp: 0.547 ± 0.141
0.82PheTyr: 0.82 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
9.241GlyAla: 9.241 ± 1.044
1.094GlyCys: 1.094 ± 0.27
6.507GlyAsp: 6.507 ± 0.575
4.648GlyGlu: 4.648 ± 0.598
2.406GlyPhe: 2.406 ± 0.472
10.335GlyGly: 10.335 ± 1.993
1.859GlyHis: 1.859 ± 0.305
4.374GlyIle: 4.374 ± 0.529
2.679GlyLys: 2.679 ± 0.395
5.796GlyLeu: 5.796 ± 0.533
2.406GlyMet: 2.406 ± 0.517
2.843GlyAsn: 2.843 ± 0.392
4.32GlyPro: 4.32 ± 0.571
2.351GlyGln: 2.351 ± 0.535
5.687GlyArg: 5.687 ± 0.599
5.632GlySer: 5.632 ± 0.841
6.179GlyThr: 6.179 ± 0.778
5.906GlyVal: 5.906 ± 0.537
2.679GlyTrp: 2.679 ± 0.392
2.133GlyTyr: 2.133 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
1.914HisAla: 1.914 ± 0.346
0.219HisCys: 0.219 ± 0.123
1.039HisAsp: 1.039 ± 0.218
1.094HisGlu: 1.094 ± 0.237
0.601HisPhe: 0.601 ± 0.162
1.859HisGly: 1.859 ± 0.293
0.766HisHis: 0.766 ± 0.25
1.367HisIle: 1.367 ± 0.311
0.766HisLys: 0.766 ± 0.226
1.586HisLeu: 1.586 ± 0.312
0.437HisMet: 0.437 ± 0.131
1.039HisAsn: 1.039 ± 0.216
1.476HisPro: 1.476 ± 0.282
0.766HisGln: 0.766 ± 0.208
2.133HisArg: 2.133 ± 0.409
1.094HisSer: 1.094 ± 0.198
1.531HisThr: 1.531 ± 0.389
1.258HisVal: 1.258 ± 0.298
0.656HisTrp: 0.656 ± 0.191
0.875HisTyr: 0.875 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
5.359IleAla: 5.359 ± 0.548
0.766IleCys: 0.766 ± 0.288
4.156IleAsp: 4.156 ± 0.478
3.5IleGlu: 3.5 ± 0.406
0.711IlePhe: 0.711 ± 0.217
3.882IleGly: 3.882 ± 0.499
1.476IleHis: 1.476 ± 0.356
1.422IleIle: 1.422 ± 0.273
1.312IleLys: 1.312 ± 0.272
2.461IleLeu: 2.461 ± 0.443
0.273IleMet: 0.273 ± 0.118
1.64IleAsn: 1.64 ± 0.304
2.625IlePro: 2.625 ± 0.294
1.312IleGln: 1.312 ± 0.256
2.351IleArg: 2.351 ± 0.412
2.406IleSer: 2.406 ± 0.385
3.609IleThr: 3.609 ± 0.46
3.007IleVal: 3.007 ± 0.411
0.875IleTrp: 0.875 ± 0.245
0.766IleTyr: 0.766 ± 0.239
0.0IleXaa: 0.0 ± 0.0
Lys
4.101LysAla: 4.101 ± 0.579
0.328LysCys: 0.328 ± 0.12
2.133LysAsp: 2.133 ± 0.29
1.422LysGlu: 1.422 ± 0.275
1.312LysPhe: 1.312 ± 0.23
2.406LysGly: 2.406 ± 0.406
1.039LysHis: 1.039 ± 0.232
1.039LysIle: 1.039 ± 0.253
1.969LysLys: 1.969 ± 0.466
2.406LysLeu: 2.406 ± 0.452
0.437LysMet: 0.437 ± 0.148
0.93LysAsn: 0.93 ± 0.258
2.461LysPro: 2.461 ± 0.408
1.64LysGln: 1.64 ± 0.273
2.461LysArg: 2.461 ± 0.423
2.242LysSer: 2.242 ± 0.367
2.461LysThr: 2.461 ± 0.434
2.461LysVal: 2.461 ± 0.403
0.656LysTrp: 0.656 ± 0.216
0.82LysTyr: 0.82 ± 0.221
0.0LysXaa: 0.0 ± 0.0
Leu
7.929LeuAla: 7.929 ± 0.883
0.656LeuCys: 0.656 ± 0.201
4.429LeuAsp: 4.429 ± 0.561
3.609LeuGlu: 3.609 ± 0.417
2.351LeuPhe: 2.351 ± 0.308
5.249LeuGly: 5.249 ± 0.511
1.039LeuHis: 1.039 ± 0.24
3.336LeuIle: 3.336 ± 0.477
2.406LeuLys: 2.406 ± 0.366
4.867LeuLeu: 4.867 ± 0.596
1.804LeuMet: 1.804 ± 0.297
2.351LeuAsn: 2.351 ± 0.375
5.413LeuPro: 5.413 ± 0.655
2.57LeuGln: 2.57 ± 0.422
5.413LeuArg: 5.413 ± 0.637
5.085LeuSer: 5.085 ± 0.533
5.468LeuThr: 5.468 ± 0.56
4.757LeuVal: 4.757 ± 0.469
1.148LeuTrp: 1.148 ± 0.248
2.297LeuTyr: 2.297 ± 0.368
0.0LeuXaa: 0.0 ± 0.0
Met
2.625MetAla: 2.625 ± 0.382
0.219MetCys: 0.219 ± 0.192
1.367MetAsp: 1.367 ± 0.298
0.875MetGlu: 0.875 ± 0.17
0.601MetPhe: 0.601 ± 0.18
1.695MetGly: 1.695 ± 0.297
0.164MetHis: 0.164 ± 0.096
0.601MetIle: 0.601 ± 0.153
0.711MetLys: 0.711 ± 0.198
1.914MetLeu: 1.914 ± 0.249
0.547MetMet: 0.547 ± 0.188
0.984MetAsn: 0.984 ± 0.21
1.094MetPro: 1.094 ± 0.241
0.492MetGln: 0.492 ± 0.143
1.476MetArg: 1.476 ± 0.259
2.789MetSer: 2.789 ± 0.424
2.078MetThr: 2.078 ± 0.381
1.531MetVal: 1.531 ± 0.329
0.273MetTrp: 0.273 ± 0.124
0.273MetTyr: 0.273 ± 0.1
0.0MetXaa: 0.0 ± 0.0
Asn
3.281AsnAla: 3.281 ± 0.381
0.164AsnCys: 0.164 ± 0.087
1.695AsnAsp: 1.695 ± 0.313
1.531AsnGlu: 1.531 ± 0.32
0.82AsnPhe: 0.82 ± 0.316
4.374AsnGly: 4.374 ± 0.629
0.875AsnHis: 0.875 ± 0.162
1.64AsnIle: 1.64 ± 0.417
0.984AsnLys: 0.984 ± 0.231
2.242AsnLeu: 2.242 ± 0.364
0.656AsnMet: 0.656 ± 0.196
1.64AsnAsn: 1.64 ± 0.341
2.242AsnPro: 2.242 ± 0.297
1.039AsnGln: 1.039 ± 0.294
2.187AsnArg: 2.187 ± 0.296
1.75AsnSer: 1.75 ± 0.309
2.023AsnThr: 2.023 ± 0.274
2.023AsnVal: 2.023 ± 0.363
0.547AsnTrp: 0.547 ± 0.15
0.711AsnTyr: 0.711 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
5.906ProAla: 5.906 ± 0.652
0.82ProCys: 0.82 ± 0.238
4.374ProAsp: 4.374 ± 0.521
4.21ProGlu: 4.21 ± 0.45
1.804ProPhe: 1.804 ± 0.343
6.616ProGly: 6.616 ± 0.641
1.258ProHis: 1.258 ± 0.262
2.133ProIle: 2.133 ± 0.314
2.133ProLys: 2.133 ± 0.449
4.429ProLeu: 4.429 ± 0.517
1.258ProMet: 1.258 ± 0.271
1.914ProAsn: 1.914 ± 0.36
4.156ProPro: 4.156 ± 0.586
2.187ProGln: 2.187 ± 0.381
3.336ProArg: 3.336 ± 0.483
3.226ProSer: 3.226 ± 0.389
3.554ProThr: 3.554 ± 0.454
4.648ProVal: 4.648 ± 0.592
1.039ProTrp: 1.039 ± 0.256
1.695ProTyr: 1.695 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
4.812GlnAla: 4.812 ± 0.628
0.273GlnCys: 0.273 ± 0.147
1.531GlnAsp: 1.531 ± 0.266
1.804GlnGlu: 1.804 ± 0.345
1.039GlnPhe: 1.039 ± 0.246
2.461GlnGly: 2.461 ± 0.429
0.984GlnHis: 0.984 ± 0.229
1.586GlnIle: 1.586 ± 0.304
1.312GlnLys: 1.312 ± 0.236
2.625GlnLeu: 2.625 ± 0.366
0.547GlnMet: 0.547 ± 0.161
1.039GlnAsn: 1.039 ± 0.273
2.734GlnPro: 2.734 ± 0.397
1.094GlnGln: 1.094 ± 0.324
2.461GlnArg: 2.461 ± 0.381
2.406GlnSer: 2.406 ± 0.414
1.531GlnThr: 1.531 ± 0.301
2.078GlnVal: 2.078 ± 0.402
0.437GlnTrp: 0.437 ± 0.137
0.93GlnTyr: 0.93 ± 0.292
0.0GlnXaa: 0.0 ± 0.0
Arg
6.562ArgAla: 6.562 ± 0.699
1.203ArgCys: 1.203 ± 0.334
4.484ArgAsp: 4.484 ± 0.569
4.812ArgGlu: 4.812 ± 0.615
2.406ArgPhe: 2.406 ± 0.381
4.757ArgGly: 4.757 ± 0.405
1.476ArgHis: 1.476 ± 0.432
4.265ArgIle: 4.265 ± 0.583
2.297ArgLys: 2.297 ± 0.429
4.374ArgLeu: 4.374 ± 0.567
2.625ArgMet: 2.625 ± 0.363
2.515ArgAsn: 2.515 ± 0.403
3.718ArgPro: 3.718 ± 0.445
2.023ArgGln: 2.023 ± 0.434
5.632ArgArg: 5.632 ± 0.789
3.718ArgSer: 3.718 ± 0.437
3.718ArgThr: 3.718 ± 0.46
5.195ArgVal: 5.195 ± 0.541
2.078ArgTrp: 2.078 ± 0.367
1.969ArgTyr: 1.969 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
5.413SerAla: 5.413 ± 0.699
0.547SerCys: 0.547 ± 0.17
4.32SerAsp: 4.32 ± 0.462
3.445SerGlu: 3.445 ± 0.455
2.023SerPhe: 2.023 ± 0.46
6.124SerGly: 6.124 ± 0.88
1.094SerHis: 1.094 ± 0.224
2.898SerIle: 2.898 ± 0.433
2.406SerLys: 2.406 ± 0.396
3.937SerLeu: 3.937 ± 0.466
1.64SerMet: 1.64 ± 0.298
2.242SerAsn: 2.242 ± 0.436
3.226SerPro: 3.226 ± 0.384
1.804SerGln: 1.804 ± 0.28
3.336SerArg: 3.336 ± 0.399
3.5SerSer: 3.5 ± 0.557
3.445SerThr: 3.445 ± 0.374
4.265SerVal: 4.265 ± 0.515
1.476SerTrp: 1.476 ± 0.263
1.422SerTyr: 1.422 ± 0.242
0.0SerXaa: 0.0 ± 0.0
Thr
7.218ThrAla: 7.218 ± 0.634
0.656ThrCys: 0.656 ± 0.22
3.937ThrAsp: 3.937 ± 0.661
3.992ThrGlu: 3.992 ± 0.449
1.914ThrPhe: 1.914 ± 0.356
5.96ThrGly: 5.96 ± 0.69
1.586ThrHis: 1.586 ± 0.282
3.718ThrIle: 3.718 ± 0.437
1.914ThrLys: 1.914 ± 0.296
4.757ThrLeu: 4.757 ± 0.598
1.258ThrMet: 1.258 ± 0.257
2.461ThrAsn: 2.461 ± 0.384
4.593ThrPro: 4.593 ± 0.738
2.133ThrGln: 2.133 ± 0.373
4.156ThrArg: 4.156 ± 0.51
3.828ThrSer: 3.828 ± 0.452
4.265ThrThr: 4.265 ± 0.429
5.468ThrVal: 5.468 ± 0.613
1.258ThrTrp: 1.258 ± 0.297
1.695ThrTyr: 1.695 ± 0.338
0.0ThrXaa: 0.0 ± 0.0
Val
7.601ValAla: 7.601 ± 0.659
1.203ValCys: 1.203 ± 0.251
5.195ValAsp: 5.195 ± 0.587
4.648ValGlu: 4.648 ± 0.634
1.914ValPhe: 1.914 ± 0.4
5.906ValGly: 5.906 ± 0.713
1.64ValHis: 1.64 ± 0.339
2.789ValIle: 2.789 ± 0.384
2.406ValLys: 2.406 ± 0.323
4.867ValLeu: 4.867 ± 0.469
1.148ValMet: 1.148 ± 0.235
2.078ValAsn: 2.078 ± 0.35
3.5ValPro: 3.5 ± 0.335
2.898ValGln: 2.898 ± 0.346
4.32ValArg: 4.32 ± 0.581
4.374ValSer: 4.374 ± 0.465
4.867ValThr: 4.867 ± 0.468
6.234ValVal: 6.234 ± 0.687
1.859ValTrp: 1.859 ± 0.333
1.367ValTyr: 1.367 ± 0.294
0.0ValXaa: 0.0 ± 0.0
Trp
1.859TrpAla: 1.859 ± 0.307
0.219TrpCys: 0.219 ± 0.103
1.422TrpAsp: 1.422 ± 0.289
1.312TrpGlu: 1.312 ± 0.383
0.547TrpPhe: 0.547 ± 0.166
1.148TrpGly: 1.148 ± 0.227
0.547TrpHis: 0.547 ± 0.175
1.148TrpIle: 1.148 ± 0.207
0.711TrpLys: 0.711 ± 0.181
1.859TrpLeu: 1.859 ± 0.322
0.875TrpMet: 0.875 ± 0.288
0.711TrpAsn: 0.711 ± 0.226
1.312TrpPro: 1.312 ± 0.339
0.984TrpGln: 0.984 ± 0.268
2.133TrpArg: 2.133 ± 0.415
1.148TrpSer: 1.148 ± 0.261
2.023TrpThr: 2.023 ± 0.368
1.695TrpVal: 1.695 ± 0.442
1.039TrpTrp: 1.039 ± 0.203
0.547TrpTyr: 0.547 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.515TyrAla: 2.515 ± 0.435
0.437TyrCys: 0.437 ± 0.199
1.476TyrAsp: 1.476 ± 0.349
1.75TyrGlu: 1.75 ± 0.273
0.711TyrPhe: 0.711 ± 0.215
1.969TyrGly: 1.969 ± 0.394
0.273TyrHis: 0.273 ± 0.112
1.094TyrIle: 1.094 ± 0.227
0.766TyrLys: 0.766 ± 0.246
2.187TyrLeu: 2.187 ± 0.308
0.109TyrMet: 0.109 ± 0.075
0.711TyrAsn: 0.711 ± 0.156
1.312TyrPro: 1.312 ± 0.239
0.656TyrGln: 0.656 ± 0.164
2.406TyrArg: 2.406 ± 0.351
0.875TyrSer: 0.875 ± 0.219
1.914TyrThr: 1.914 ± 0.365
2.406TyrVal: 2.406 ± 0.358
0.656TyrTrp: 0.656 ± 0.142
0.656TyrTyr: 0.656 ± 0.177
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (18289 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski