Rheinheimera phage vB_RspM_Barba9A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Barbavirus; unclassified Barbavirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8MZI5|A0A4P8MZI5_9CAUD Uncharacterized protein OS=Rheinheimera phage vB_RspM_Barba9A OX=2565697 GN=Barba9A_gp059 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 6.46KK3 pKa = 10.48YY4 pKa = 10.21DD5 pKa = 4.16CPDD8 pKa = 3.2NGVTEE13 pKa = 3.97ICNNYY18 pKa = 10.15DD19 pKa = 3.56VIAIQINGNDD29 pKa = 3.8KK30 pKa = 10.81LVTMAEE36 pKa = 4.0NLLKK40 pKa = 10.81GFYY43 pKa = 10.28GDD45 pKa = 2.96NWSLEE50 pKa = 4.1RR51 pKa = 11.84ATEE54 pKa = 3.91LRR56 pKa = 11.84AIYY59 pKa = 9.77PNYY62 pKa = 10.67DD63 pKa = 3.31DD64 pKa = 6.53FRR66 pKa = 11.84DD67 pKa = 3.63TKK69 pKa = 9.73YY70 pKa = 9.27TVHH73 pKa = 7.0EE74 pKa = 4.26LTDD77 pKa = 3.73LVIDD81 pKa = 4.88DD82 pKa = 4.29EE83 pKa = 4.6

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8N055|A0A4P8N055_9CAUD Uncharacterized protein OS=Rheinheimera phage vB_RspM_Barba9A OX=2565697 GN=Barba9A_gp071 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.37RR3 pKa = 11.84KK4 pKa = 10.04LIQRR8 pKa = 11.84NKK10 pKa = 9.23QFLSSRR16 pKa = 11.84YY17 pKa = 7.63KK18 pKa = 10.62HH19 pKa = 6.26NYY21 pKa = 8.31SGNGSFQRR29 pKa = 11.84IRR31 pKa = 11.84VHH33 pKa = 6.8KK34 pKa = 9.94PFIDD38 pKa = 3.47SHH40 pKa = 7.9GEE42 pKa = 3.66LQIRR46 pKa = 11.84KK47 pKa = 8.55PRR49 pKa = 11.84YY50 pKa = 6.44IWRR53 pKa = 11.84WFFNAEE59 pKa = 3.87WFSDD63 pKa = 3.7LVGWFSDD70 pKa = 3.94LRR72 pKa = 11.84GEE74 pKa = 4.37VHH76 pKa = 7.43DD77 pKa = 4.56

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

138

0

138

24100

24

950

174.6

19.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.174 ± 0.377

1.461 ± 0.112

6.311 ± 0.202

6.436 ± 0.227

4.278 ± 0.144

6.618 ± 0.262

1.581 ± 0.105

6.174 ± 0.182

6.838 ± 0.299

8.357 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.143

5.751 ± 0.202

3.237 ± 0.183

3.863 ± 0.143

4.033 ± 0.138

6.755 ± 0.248

6.581 ± 0.347

7.195 ± 0.207

1.407 ± 0.099

4.49 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski