Desulfuromonas acetexigens
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3347 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A550JHF4|A0A550JHF4_9DELT Ribonuclease VapC OS=Desulfuromonas acetexigens OX=38815 GN=vapC PE=3 SV=1
MM1 pKa = 8.1 RR2 pKa = 11.84 STYY5 pKa = 9.07 WKK7 pKa = 10.58 GVFLAWFVLALVTPCLGSSITFEE30 pKa = 3.99 ATNLNDD36 pKa = 4.11 SGGADD41 pKa = 3.05 RR42 pKa = 11.84 WAYY45 pKa = 10.07 SYY47 pKa = 10.82 RR48 pKa = 11.84 VSGNFNMFSGFTVYY62 pKa = 10.63 FGVGDD67 pKa = 3.94 SANISASNPQDD78 pKa = 3.66 LGDD81 pKa = 3.66 WDD83 pKa = 4.09 VLTWEE88 pKa = 5.18 PDD90 pKa = 3.13 PGIPDD95 pKa = 4.04 DD96 pKa = 4.08 GAYY99 pKa = 10.75 DD100 pKa = 4.18 AMALVDD106 pKa = 3.94 NASLNSVFNVGFDD119 pKa = 3.41 WMGSSAPGSQYY130 pKa = 10.87 FEE132 pKa = 4.75 IYY134 pKa = 10.52 DD135 pKa = 3.66 EE136 pKa = 4.29 NFQILEE142 pKa = 4.05 SGYY145 pKa = 7.26 TTLALSAPIATPEE158 pKa = 4.4 PGTFLLVGFGGVALLIFKK176 pKa = 9.78 FRR178 pKa = 11.84 KK179 pKa = 10.02 SNTEE183 pKa = 3.42 AA184 pKa = 3.75
Molecular weight: 19.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A550J5N0|A0A550J5N0_9DELT Cation transporter OS=Desulfuromonas acetexigens OX=38815 GN=FL622_16165 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.32 QPSNVKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 7.89 RR14 pKa = 11.84 THH16 pKa = 5.86 GFRR19 pKa = 11.84 KK20 pKa = 10.07 RR21 pKa = 11.84 MQTANGRR28 pKa = 11.84 IVIKK32 pKa = 10.32 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.36 GRR39 pKa = 11.84 KK40 pKa = 8.32 RR41 pKa = 11.84 LVVSIPVKK49 pKa = 10.61
Molecular weight: 5.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3347
0
3347
1093803
34
2505
326.8
36.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.216 ± 0.054
1.224 ± 0.027
5.353 ± 0.03
7.026 ± 0.043
3.999 ± 0.031
8.17 ± 0.04
2.114 ± 0.029
5.246 ± 0.038
4.039 ± 0.042
11.611 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.02
2.824 ± 0.026
4.916 ± 0.032
3.352 ± 0.022
7.157 ± 0.047
5.114 ± 0.032
4.865 ± 0.031
6.987 ± 0.034
1.079 ± 0.015
2.549 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here