Capybara microvirus Cap1_SP_123
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVP2|A0A4V1FVP2_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_123 OX=2585378 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.45 VFEE5 pKa = 4.64 GFEE8 pKa = 4.47 KK9 pKa = 10.55 INSFRR14 pKa = 11.84 IVAIPDD20 pKa = 3.35 AKK22 pKa = 10.59 RR23 pKa = 11.84 LFEE26 pKa = 5.0 LYY28 pKa = 9.68 RR29 pKa = 11.84 HH30 pKa = 5.97 SPNLVGNEE38 pKa = 4.09 EE39 pKa = 4.06 EE40 pKa = 4.65 TIDD43 pKa = 4.68 LALEE47 pKa = 4.21 SAQRR51 pKa = 11.84 PTDD54 pKa = 3.37 SMALYY59 pKa = 8.67 EE60 pKa = 4.11 QYY62 pKa = 11.0 ARR64 pKa = 11.84 SMDD67 pKa = 3.75 SEE69 pKa = 4.41
Molecular weight: 8.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.786
IPC2_protein 4.8
IPC_protein 4.622
Toseland 4.482
ProMoST 4.596
Dawson 4.546
Bjellqvist 4.762
Wikipedia 4.393
Rodwell 4.457
Grimsley 4.393
Solomon 4.546
Lehninger 4.495
Nozaki 4.66
DTASelect 4.749
Thurlkill 4.482
EMBOSS 4.418
Sillero 4.724
Patrickios 4.075
IPC_peptide 4.546
IPC2_peptide 4.724
IPC2.peptide.svr19 4.655
Protein with the highest isoelectric point:
>tr|A0A4P8W464|A0A4P8W464_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_123 OX=2585378 PE=4 SV=1
MM1 pKa = 7.71 CISPRR6 pKa = 11.84 IIPNPYY12 pKa = 10.33 YY13 pKa = 10.34 NDD15 pKa = 3.64 KK16 pKa = 11.59 GNTAWLHH23 pKa = 5.58 DD24 pKa = 4.41 TVCSHH29 pKa = 6.14 IRR31 pKa = 11.84 APCGHH36 pKa = 6.73 CPEE39 pKa = 5.68 CIHH42 pKa = 6.48 NKK44 pKa = 8.01 QNYY47 pKa = 6.13 WVQRR51 pKa = 11.84 CQMMEE56 pKa = 4.29 FDD58 pKa = 4.91 YY59 pKa = 10.81 IPYY62 pKa = 9.35 MVTLTYY68 pKa = 10.75 NHH70 pKa = 7.31 NIRR73 pKa = 11.84 KK74 pKa = 8.74 LHH76 pKa = 6.22 IGDD79 pKa = 3.84 YY80 pKa = 10.74 SIMYY84 pKa = 9.9 ADD86 pKa = 4.08 KK87 pKa = 11.06 TDD89 pKa = 3.23 IQNMFKK95 pKa = 10.74 RR96 pKa = 11.84 MRR98 pKa = 11.84 KK99 pKa = 7.78 YY100 pKa = 10.82 DD101 pKa = 3.34 VFGRR105 pKa = 11.84 EE106 pKa = 3.69 FKK108 pKa = 10.98 YY109 pKa = 10.39 IAVSEE114 pKa = 4.33 RR115 pKa = 11.84 GKK117 pKa = 8.88 EE118 pKa = 3.69 THH120 pKa = 6.41 RR121 pKa = 11.84 PHH123 pKa = 5.09 WHH125 pKa = 5.83 ILFFLKK131 pKa = 10.52 KK132 pKa = 9.92 LANEE136 pKa = 4.37 NEE138 pKa = 3.73 WSYY141 pKa = 11.42 ISHH144 pKa = 6.3 EE145 pKa = 4.8 KK146 pKa = 10.06 EE147 pKa = 3.8 LQSIFLNYY155 pKa = 6.24 WTRR158 pKa = 11.84 NYY160 pKa = 10.92 GSTRR164 pKa = 11.84 KK165 pKa = 9.24 PDD167 pKa = 3.81 YY168 pKa = 10.81 KK169 pKa = 10.77 PLLTLNKK176 pKa = 9.51 RR177 pKa = 11.84 RR178 pKa = 11.84 TDD180 pKa = 2.92 GRR182 pKa = 11.84 RR183 pKa = 11.84 NFDD186 pKa = 3.36 LHH188 pKa = 7.77 YY189 pKa = 10.59 CMPGPSGNDD198 pKa = 2.86 DD199 pKa = 3.4 VLFYY203 pKa = 9.21 CTKK206 pKa = 10.76 YY207 pKa = 10.27 ILKK210 pKa = 9.78 HH211 pKa = 6.1 DD212 pKa = 4.12 PYY214 pKa = 10.97 EE215 pKa = 4.7 RR216 pKa = 11.84 KK217 pKa = 9.48 LHH219 pKa = 5.58 SALMLNYY226 pKa = 9.99 EE227 pKa = 3.89 NDD229 pKa = 3.37 EE230 pKa = 4.33 AKK232 pKa = 10.75 SYY234 pKa = 10.12 WNTVKK239 pKa = 10.68 SRR241 pKa = 11.84 MLVSHH246 pKa = 6.69 GFGTPYY252 pKa = 9.68 TLSYY256 pKa = 10.52 EE257 pKa = 4.13 DD258 pKa = 4.31 KK259 pKa = 10.59 KK260 pKa = 11.36 VKK262 pKa = 8.99 KK263 pKa = 8.47 TPNPSVIRR271 pKa = 11.84 HH272 pKa = 4.97 IRR274 pKa = 11.84 KK275 pKa = 9.52 GLDD278 pKa = 3.14 LSTKK282 pKa = 8.62 MPLYY286 pKa = 10.35 INPNNGYY293 pKa = 7.86 TMPLSPIYY301 pKa = 10.0 KK302 pKa = 7.34 PHH304 pKa = 6.57 CMTRR308 pKa = 11.84 EE309 pKa = 4.15 DD310 pKa = 3.81 YY311 pKa = 10.88 QKK313 pKa = 10.78 QFDD316 pKa = 4.33 NNNLIDD322 pKa = 3.96 INGDD326 pKa = 3.75 KK327 pKa = 10.33 INHH330 pKa = 6.25 GSCFIDD336 pKa = 3.55 TSTKK340 pKa = 10.22 DD341 pKa = 3.23 YY342 pKa = 11.3 EE343 pKa = 4.61 RR344 pKa = 11.84 IIDD347 pKa = 3.88 QFNHH351 pKa = 7.49 RR352 pKa = 11.84 ILQINKK358 pKa = 9.38 HH359 pKa = 4.67 YY360 pKa = 11.09
Molecular weight: 43.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.546
IPC2_protein 8.507
IPC_protein 8.361
Toseland 8.712
ProMoST 8.873
Dawson 9.165
Bjellqvist 9.151
Wikipedia 9.341
Rodwell 9.268
Grimsley 9.151
Solomon 9.224
Lehninger 9.18
Nozaki 9.194
DTASelect 9.019
Thurlkill 9.077
EMBOSS 9.282
Sillero 9.282
Patrickios 3.999
IPC_peptide 9.209
IPC2_peptide 7.98
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1624
69
635
270.7
30.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.404 ± 1.905
1.478 ± 0.581
5.603 ± 0.577
5.172 ± 0.89
4.187 ± 1.124
4.372 ± 0.529
2.586 ± 0.948
6.897 ± 0.745
6.096 ± 1.452
7.451 ± 0.267
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.463 ± 0.36
8.067 ± 0.331
3.941 ± 0.596
4.31 ± 0.866
4.988 ± 0.526
8.682 ± 1.297
6.219 ± 1.2
4.31 ± 1.064
0.677 ± 0.296
6.096 ± 0.872
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here