Noviherbaspirillum galbum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Noviherbaspirillum

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B3SUH3|A0A6B3SUH3_9BURK Ubiquinol-cytochrome c reductase iron-sulfur subunit OS=Noviherbaspirillum galbum OX=2709383 GN=petA PE=4 SV=1
MM1 pKa = 7.15NAVTEE6 pKa = 4.35MPAPIVFTDD15 pKa = 3.76SAADD19 pKa = 3.62KK20 pKa = 10.23VAQLIAEE27 pKa = 4.52EE28 pKa = 4.68GNPDD32 pKa = 2.99LKK34 pKa = 11.13LRR36 pKa = 11.84VFVQGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 4.69EE55 pKa = 4.93IVNEE59 pKa = 5.02DD60 pKa = 4.04DD61 pKa = 3.2TTMTKK66 pKa = 10.66NGVQLLIDD74 pKa = 3.77SMSYY78 pKa = 10.23QYY80 pKa = 11.55LVGAEE85 pKa = 4.01IDD87 pKa = 3.89YY88 pKa = 11.28KK89 pKa = 11.39DD90 pKa = 4.47DD91 pKa = 4.08LEE93 pKa = 4.56GAQFVIKK100 pKa = 10.5NPNATTTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B3ST16|A0A6B3ST16_9BURK Thioesterase OS=Noviherbaspirillum galbum OX=2709383 GN=G3574_22735 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.19RR14 pKa = 11.84THH16 pKa = 5.61GFRR19 pKa = 11.84VRR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5472

0

5472

1783336

29

4901

325.9

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.165 ± 0.051

0.934 ± 0.011

5.435 ± 0.025

5.482 ± 0.033

3.605 ± 0.022

8.139 ± 0.03

2.236 ± 0.018

4.925 ± 0.023

3.754 ± 0.029

10.267 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.712 ± 0.016

3.003 ± 0.023

4.978 ± 0.028

3.86 ± 0.025

6.791 ± 0.036

5.722 ± 0.028

5.097 ± 0.029

7.249 ± 0.026

1.286 ± 0.015

2.359 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski