Escherichia phage T1 (Bacteriophage T1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tunavirinae; Tunavirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6XQE8|Q6XQE8_BPT1 Uncharacterized protein OS=Escherichia phage T1 OX=1921008 GN=62 PE=4 SV=1
MM1 pKa = 7.06MVSTDD6 pKa = 4.43KK7 pKa = 11.14FFTCTKK13 pKa = 7.72TSEE16 pKa = 4.26VFEE19 pKa = 4.93LVHH22 pKa = 6.35TDD24 pKa = 3.22NGDD27 pKa = 3.48FMHH30 pKa = 7.35DD31 pKa = 3.21GCDD34 pKa = 3.08AFIEE38 pKa = 4.6VKK40 pKa = 10.57EE41 pKa = 4.01SDD43 pKa = 3.89YY44 pKa = 11.82DD45 pKa = 3.81DD46 pKa = 3.5GVYY49 pKa = 10.86YY50 pKa = 10.88NPAVNTQFFTPIEE63 pKa = 4.26EE64 pKa = 4.39EE65 pKa = 4.39GEE67 pKa = 4.17EE68 pKa = 4.09AA69 pKa = 5.17

Molecular weight: 7.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6XQF0|Q6XQF0_BPT1 Putative HNH endonuclease OS=Escherichia phage T1 OX=1921008 GN=63 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 5.82GDD4 pKa = 3.57ITNNRR9 pKa = 11.84IEE11 pKa = 4.14NLRR14 pKa = 11.84LVTRR18 pKa = 11.84TINNRR23 pKa = 11.84NQKK26 pKa = 9.49KK27 pKa = 10.16RR28 pKa = 11.84SDD30 pKa = 3.55NTTGVSGVTFMKK42 pKa = 10.74DD43 pKa = 2.5RR44 pKa = 11.84GKK46 pKa = 10.91YY47 pKa = 8.55RR48 pKa = 11.84AQVRR52 pKa = 11.84NKK54 pKa = 10.37RR55 pKa = 11.84LGQFDD60 pKa = 4.57TIEE63 pKa = 4.03EE64 pKa = 3.86AAKK67 pKa = 10.28AVKK70 pKa = 10.2DD71 pKa = 3.57EE72 pKa = 4.12RR73 pKa = 11.84DD74 pKa = 3.38RR75 pKa = 11.84LGLFTKK81 pKa = 10.53RR82 pKa = 11.84HH83 pKa = 4.81GVV85 pKa = 3.18

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

2

80

15068

36

1172

188.3

21.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.103 ± 0.39

1.294 ± 0.146

6.179 ± 0.203

6.63 ± 0.238

3.876 ± 0.153

7.174 ± 0.236

1.805 ± 0.216

6.776 ± 0.211

7.035 ± 0.3

6.464 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.073 ± 0.153

5.044 ± 0.284

3.285 ± 0.179

3.776 ± 0.255

5.004 ± 0.275

6.769 ± 0.283

5.648 ± 0.259

7.154 ± 0.242

1.42 ± 0.11

3.491 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski