Porphyromonas sp. oral taxon 279 str. F0450
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1729 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J6GYR0|J6GYR0_9PORP Uncharacterized protein OS=Porphyromonas sp. oral taxon 279 str. F0450 OX=1125723 GN=HMPREF1323_1005 PE=4 SV=1
MM1 pKa = 7.56 GNCSLASLGKK11 pKa = 9.11 PLPINIMVEE20 pKa = 4.16 DD21 pKa = 4.29 EE22 pKa = 4.21 LLGYY26 pKa = 10.77 DD27 pKa = 3.9 DD28 pKa = 4.01 TASVAFIRR36 pKa = 11.84 NYY38 pKa = 9.66 IPQEE42 pKa = 3.94 LKK44 pKa = 10.88 EE45 pKa = 4.52 LLSDD49 pKa = 3.78 DD50 pKa = 4.98 DD51 pKa = 3.41 IVYY54 pKa = 9.89 FVDD57 pKa = 6.12 LIYY60 pKa = 10.54 DD61 pKa = 3.81 YY62 pKa = 11.34 YY63 pKa = 10.93 EE64 pKa = 4.07 SRR66 pKa = 11.84 GYY68 pKa = 11.14 LSDD71 pKa = 5.79 DD72 pKa = 3.67 DD73 pKa = 4.68 TDD75 pKa = 4.04 DD76 pKa = 4.27 EE77 pKa = 4.59 EE78 pKa = 6.52 PIEE81 pKa = 4.26 IDD83 pKa = 3.0 EE84 pKa = 5.18 DD85 pKa = 3.96 EE86 pKa = 4.48 LVDD89 pKa = 3.77 YY90 pKa = 9.87 VVRR93 pKa = 11.84 NAQKK97 pKa = 10.87 DD98 pKa = 4.08 GVGKK102 pKa = 9.34 FAPDD106 pKa = 3.12 QIRR109 pKa = 11.84 FVVQGEE115 pKa = 4.24 LEE117 pKa = 4.18 YY118 pKa = 11.3 CEE120 pKa = 5.06 SINLFDD126 pKa = 4.14
Molecular weight: 14.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.592
IPC_protein 3.579
Toseland 3.363
ProMoST 3.719
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.528
Rodwell 3.414
Grimsley 3.274
Solomon 3.567
Lehninger 3.528
Nozaki 3.706
DTASelect 3.935
Thurlkill 3.427
EMBOSS 3.541
Sillero 3.706
Patrickios 0.655
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.706
Protein with the highest isoelectric point:
>tr|J6H436|J6H436_9PORP EamA-like transporter family protein OS=Porphyromonas sp. oral taxon 279 str. F0450 OX=1125723 GN=HMPREF1323_0975 PE=4 SV=1
MM1 pKa = 6.83 NTQAMNHH8 pKa = 6.7 DD9 pKa = 3.56 PHH11 pKa = 8.45 SPVFIRR17 pKa = 11.84 GKK19 pKa = 10.46 ASPDD23 pKa = 3.29 KK24 pKa = 9.04 QTKK27 pKa = 8.94 GQVSPSRR34 pKa = 11.84 FVDD37 pKa = 2.87 WRR39 pKa = 11.84 RR40 pKa = 11.84 IIEE43 pKa = 4.19 LALLLTLVVAAVYY56 pKa = 10.0 LISRR60 pKa = 11.84 VVTPQVVTIVGVIAGFLLLRR80 pKa = 11.84 FVVRR84 pKa = 11.84 TILQVTFTILGYY96 pKa = 10.16 LLWLAIFVAILICILL111 pKa = 3.56
Molecular weight: 12.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.672
ProMoST 10.394
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.965
Grimsley 10.833
Solomon 10.906
Lehninger 10.877
Nozaki 10.657
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.774
IPC_peptide 10.906
IPC2_peptide 9.589
IPC2.peptide.svr19 8.473
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1729
0
1729
641230
36
2373
370.9
41.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.595 ± 0.052
0.868 ± 0.02
5.284 ± 0.036
6.656 ± 0.063
4.016 ± 0.034
7.266 ± 0.042
2.164 ± 0.029
5.936 ± 0.043
4.891 ± 0.047
10.655 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.126 ± 0.026
3.235 ± 0.05
4.432 ± 0.032
3.657 ± 0.029
6.196 ± 0.047
6.701 ± 0.046
5.949 ± 0.044
6.12 ± 0.04
1.096 ± 0.019
4.158 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here