Ground squirrel hepatitis virus (strain 27) (GSHV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Orthohepadnavirus

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03144-3|HBSAG-3_GSHV Isoform of P03144 Isoform S of Large envelope protein OS=Ground squirrel hepatitis virus (strain 27) OX=10406 GN=S PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 10.24NQTGHH7 pKa = 6.09LQGFAEE13 pKa = 4.79GLRR16 pKa = 11.84ALTTSDD22 pKa = 3.51HH23 pKa = 6.63HH24 pKa = 6.52NSAYY28 pKa = 10.43GDD30 pKa = 3.82PFTTLSPVVPTVSTTLSPPLTIGDD54 pKa = 4.02PVLSTEE60 pKa = 4.3MSPSGLLGLLAGLQVVYY77 pKa = 8.81FLWTKK82 pKa = 10.34ILTIAQSLDD91 pKa = 3.07WWWTSLSFPGGIPEE105 pKa = 4.17CTGQNLQFQTCKK117 pKa = 10.28HH118 pKa = 6.29LPTSCPPTCNGFRR131 pKa = 11.84WMYY134 pKa = 9.79LRR136 pKa = 11.84RR137 pKa = 11.84FIIYY141 pKa = 10.35LLVLLLFLTFLLVLLDD157 pKa = 3.83WKK159 pKa = 11.02GLLPVCPMMPATEE172 pKa = 4.2TTVNCRR178 pKa = 11.84QCTISAQDD186 pKa = 3.43TFTTPYY192 pKa = 9.77CCCLKK197 pKa = 9.41PTAGNCTCWPIPSSWALGSYY217 pKa = 9.13LWEE220 pKa = 3.58WALARR225 pKa = 11.84FSWLSLLVPLLQWLGGISLTVWLLLIWMIWFWGPVLMSILPPFIPIFALFFLIWAYY281 pKa = 9.91II282 pKa = 3.43

Molecular weight: 31.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03144-2|HBSAG-2_GSHV Isoform of P03144 Isoform M of Large envelope protein OS=Ground squirrel hepatitis virus (strain 27) OX=10406 GN=S PE=3 SV=1
MM1 pKa = 8.03DD2 pKa = 5.37IDD4 pKa = 4.05PYY6 pKa = 11.45KK7 pKa = 10.72EE8 pKa = 4.12FGSSYY13 pKa = 10.8QLLNFLPLDD22 pKa = 3.88FFPDD26 pKa = 3.91LNALVDD32 pKa = 3.96TAAALYY38 pKa = 10.25EE39 pKa = 4.12EE40 pKa = 4.9EE41 pKa = 4.22LTGRR45 pKa = 11.84EE46 pKa = 4.29HH47 pKa = 7.77CSPHH51 pKa = 5.3HH52 pKa = 4.74TAIRR56 pKa = 11.84QALVCWEE63 pKa = 4.11EE64 pKa = 4.01LTRR67 pKa = 11.84LITWMSEE74 pKa = 3.44NTTEE78 pKa = 3.99EE79 pKa = 3.73VRR81 pKa = 11.84RR82 pKa = 11.84IIVDD86 pKa = 3.38HH87 pKa = 6.85VNNTWGLKK95 pKa = 9.12VRR97 pKa = 11.84QTLWFHH103 pKa = 6.52LSCLTFGQHH112 pKa = 5.07TVQEE116 pKa = 4.43FLVSFGVWIRR126 pKa = 11.84TPAPYY131 pKa = 9.94RR132 pKa = 11.84PPNAPILSTLPEE144 pKa = 3.9HH145 pKa = 5.34TVIRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84GGSRR155 pKa = 11.84AARR158 pKa = 11.84SPRR161 pKa = 11.84RR162 pKa = 11.84RR163 pKa = 11.84TPSPRR168 pKa = 11.84RR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84SQSPRR176 pKa = 11.84RR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84SQSPASNCC187 pKa = 3.53

Molecular weight: 21.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

2

7

2355

138

881

336.4

38.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.605 ± 0.428

3.312 ± 0.443

3.057 ± 0.331

3.015 ± 0.558

5.011 ± 0.223

5.563 ± 0.393

2.718 ± 0.562

4.926 ± 0.429

3.142 ± 0.741

13.079 ± 1.146

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.741 ± 0.342

3.779 ± 0.714

7.771 ± 0.656

3.907 ± 0.102

5.902 ± 1.253

7.813 ± 0.547

7.983 ± 0.679

4.841 ± 0.179

3.864 ± 0.743

2.972 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski