Eubacterium sp. CAG:76

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2278 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7NBV4|R7NBV4_9FIRM Putative manganese efflux pump MntP OS=Eubacterium sp. CAG:76 OX=1262892 GN=mntP PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.26KK3 pKa = 10.33KK4 pKa = 10.32LVSLMLVAAMTASLTACGSKK24 pKa = 8.77KK25 pKa = 8.96TEE27 pKa = 4.04EE28 pKa = 4.3PTSTVHH34 pKa = 7.42KK35 pKa = 10.14SITAAEE41 pKa = 3.92YY42 pKa = 10.49DD43 pKa = 3.48AAITSDD49 pKa = 2.85AAIYY53 pKa = 10.13KK54 pKa = 10.12KK55 pKa = 10.53AFTMPEE61 pKa = 3.81YY62 pKa = 10.59KK63 pKa = 10.42GIQVTVDD70 pKa = 3.22KK71 pKa = 11.2SVLNVAEE78 pKa = 4.68SDD80 pKa = 3.3VDD82 pKa = 4.75DD83 pKa = 5.1YY84 pKa = 11.81INKK87 pKa = 10.0NIVSAEE93 pKa = 3.79ATTEE97 pKa = 4.01NVTSGVTADD106 pKa = 3.92GDD108 pKa = 4.54TVILDD113 pKa = 3.58YY114 pKa = 11.09SGKK117 pKa = 10.3LDD119 pKa = 3.68GEE121 pKa = 4.37AFSGGTATDD130 pKa = 3.3TTYY133 pKa = 10.8TIGSGNFISDD143 pKa = 3.68LDD145 pKa = 3.84KK146 pKa = 11.56GLVGLTVGQEE156 pKa = 3.74YY157 pKa = 10.23DD158 pKa = 3.64IPCTFPDD165 pKa = 4.27NYY167 pKa = 10.67TSDD170 pKa = 3.92LAGKK174 pKa = 9.59SVIFTVKK181 pKa = 8.57VTAIQKK187 pKa = 5.41TTYY190 pKa = 10.48PEE192 pKa = 4.03ITDD195 pKa = 3.11EE196 pKa = 3.95WVVNNKK202 pKa = 9.59EE203 pKa = 4.18SLNLSGDD210 pKa = 3.54TVADD214 pKa = 3.87FRR216 pKa = 11.84EE217 pKa = 4.24EE218 pKa = 3.56VRR220 pKa = 11.84KK221 pKa = 9.9IVEE224 pKa = 3.85ANANDD229 pKa = 3.89TYY231 pKa = 11.55LNNTFTSAYY240 pKa = 9.94QIISEE245 pKa = 4.46NIGDD249 pKa = 3.63VNYY252 pKa = 9.65PQDD255 pKa = 4.3EE256 pKa = 4.19IDD258 pKa = 4.1SLVEE262 pKa = 3.73VLNTNIEE269 pKa = 4.51SEE271 pKa = 4.31FNTYY275 pKa = 9.41GSYY278 pKa = 11.03YY279 pKa = 10.25GISDD283 pKa = 4.41LDD285 pKa = 3.85TYY287 pKa = 10.79KK288 pKa = 10.81KK289 pKa = 10.29SVYY292 pKa = 10.54GFDD295 pKa = 4.77SIDD298 pKa = 3.43AFNEE302 pKa = 4.1YY303 pKa = 9.08ATSSAQQYY311 pKa = 9.89LLQKK315 pKa = 10.1MIVTVIAADD324 pKa = 3.61NDD326 pKa = 3.46IHH328 pKa = 8.6VSEE331 pKa = 5.52DD332 pKa = 3.56EE333 pKa = 4.15INSYY337 pKa = 11.54GNDD340 pKa = 3.0LAQYY344 pKa = 10.15YY345 pKa = 10.76GYY347 pKa = 10.74DD348 pKa = 3.66DD349 pKa = 4.53FNAIVDD355 pKa = 3.97AVGSEE360 pKa = 4.32VVSEE364 pKa = 4.04IGYY367 pKa = 9.68EE368 pKa = 3.62ILYY371 pKa = 9.74QKK373 pKa = 10.5VVEE376 pKa = 4.92FEE378 pKa = 4.22CSQITEE384 pKa = 4.31VEE386 pKa = 4.1QQ387 pKa = 3.78

Molecular weight: 42.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7NBP4|R7NBP4_9FIRM tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase OS=Eubacterium sp. CAG:76 OX=1262892 GN=miaB PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.78TRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.82VHH16 pKa = 5.97GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2278

0

2278

740048

29

2413

324.9

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.165 ± 0.049

1.488 ± 0.022

6.453 ± 0.041

6.786 ± 0.047

3.758 ± 0.038

6.596 ± 0.048

1.501 ± 0.022

8.579 ± 0.044

7.374 ± 0.045

8.048 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.108 ± 0.025

5.792 ± 0.043

2.729 ± 0.026

2.695 ± 0.026

3.798 ± 0.033

6.228 ± 0.049

5.426 ± 0.046

7.169 ± 0.046

0.713 ± 0.016

4.595 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski