Amino acid dipepetide frequency for Enterobacteria phage IME_EC2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.687AlaAla: 13.687 ± 2.158
0.391AlaCys: 0.391 ± 0.166
7.195AlaAsp: 7.195 ± 0.883
7.508AlaGlu: 7.508 ± 0.834
3.285AlaPhe: 3.285 ± 0.537
9.151AlaGly: 9.151 ± 1.331
1.799AlaHis: 1.799 ± 0.368
6.491AlaIle: 6.491 ± 0.638
5.475AlaLys: 5.475 ± 0.696
9.229AlaLeu: 9.229 ± 0.926
2.737AlaMet: 2.737 ± 0.42
4.38AlaAsn: 4.38 ± 0.743
4.145AlaPro: 4.145 ± 0.673
6.179AlaGln: 6.179 ± 1.082
7.743AlaArg: 7.743 ± 0.909
5.631AlaSer: 5.631 ± 0.68
7.195AlaThr: 7.195 ± 1.027
5.318AlaVal: 5.318 ± 0.622
1.251AlaTrp: 1.251 ± 0.283
2.894AlaTyr: 2.894 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
0.939CysAla: 0.939 ± 0.326
0.156CysCys: 0.156 ± 0.11
0.547CysAsp: 0.547 ± 0.256
0.704CysGlu: 0.704 ± 0.273
0.391CysPhe: 0.391 ± 0.22
1.095CysGly: 1.095 ± 0.345
0.391CysHis: 0.391 ± 0.19
0.235CysIle: 0.235 ± 0.139
0.626CysLys: 0.626 ± 0.21
0.547CysLeu: 0.547 ± 0.191
0.156CysMet: 0.156 ± 0.116
0.156CysAsn: 0.156 ± 0.104
0.391CysPro: 0.391 ± 0.159
0.078CysGln: 0.078 ± 0.069
1.408CysArg: 1.408 ± 0.395
0.235CysSer: 0.235 ± 0.139
0.235CysThr: 0.235 ± 0.197
0.626CysVal: 0.626 ± 0.209
0.078CysTrp: 0.078 ± 0.093
0.235CysTyr: 0.235 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
6.883AspAla: 6.883 ± 0.85
0.469AspCys: 0.469 ± 0.196
3.989AspAsp: 3.989 ± 0.666
5.475AspGlu: 5.475 ± 0.689
1.486AspPhe: 1.486 ± 0.411
5.709AspGly: 5.709 ± 0.714
1.486AspHis: 1.486 ± 0.383
2.659AspIle: 2.659 ± 0.515
3.05AspLys: 3.05 ± 0.569
5.631AspLeu: 5.631 ± 0.659
1.33AspMet: 1.33 ± 0.331
2.894AspAsn: 2.894 ± 0.536
4.38AspPro: 4.38 ± 0.677
3.598AspGln: 3.598 ± 0.695
3.441AspArg: 3.441 ± 0.534
2.581AspSer: 2.581 ± 0.434
2.894AspThr: 2.894 ± 0.449
3.989AspVal: 3.989 ± 0.587
2.425AspTrp: 2.425 ± 0.511
1.721AspTyr: 1.721 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
8.994GluAla: 8.994 ± 0.983
0.939GluCys: 0.939 ± 0.281
4.145GluAsp: 4.145 ± 0.637
4.067GluGlu: 4.067 ± 0.548
2.268GluPhe: 2.268 ± 0.48
3.832GluGly: 3.832 ± 0.587
0.86GluHis: 0.86 ± 0.302
3.676GluIle: 3.676 ± 0.675
5.24GluLys: 5.24 ± 0.639
5.475GluLeu: 5.475 ± 0.812
2.346GluMet: 2.346 ± 0.384
2.19GluAsn: 2.19 ± 0.513
3.05GluPro: 3.05 ± 0.541
3.207GluGln: 3.207 ± 0.609
4.302GluArg: 4.302 ± 0.71
3.754GluSer: 3.754 ± 0.544
2.737GluThr: 2.737 ± 0.419
3.441GluVal: 3.441 ± 0.539
1.486GluTrp: 1.486 ± 0.335
1.721GluTyr: 1.721 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
2.894PheAla: 2.894 ± 0.48
0.156PheCys: 0.156 ± 0.118
2.033PheAsp: 2.033 ± 0.376
2.033PheGlu: 2.033 ± 0.431
1.095PhePhe: 1.095 ± 0.352
2.894PheGly: 2.894 ± 0.414
0.547PheHis: 0.547 ± 0.231
1.251PheIle: 1.251 ± 0.299
1.642PheLys: 1.642 ± 0.367
2.503PheLeu: 2.503 ± 0.416
1.095PheMet: 1.095 ± 0.333
1.721PheAsn: 1.721 ± 0.312
1.173PhePro: 1.173 ± 0.277
1.017PheGln: 1.017 ± 0.305
2.425PheArg: 2.425 ± 0.346
1.799PheSer: 1.799 ± 0.342
0.939PheThr: 0.939 ± 0.222
2.033PheVal: 2.033 ± 0.36
1.095PheTrp: 1.095 ± 0.323
0.86PheTyr: 0.86 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
7.977GlyAla: 7.977 ± 1.232
0.469GlyCys: 0.469 ± 0.178
5.005GlyAsp: 5.005 ± 0.6
5.318GlyGlu: 5.318 ± 0.627
2.346GlyPhe: 2.346 ± 0.483
8.134GlyGly: 8.134 ± 1.109
0.939GlyHis: 0.939 ± 0.317
4.614GlyIle: 4.614 ± 0.663
4.223GlyLys: 4.223 ± 0.718
5.005GlyLeu: 5.005 ± 0.706
2.112GlyMet: 2.112 ± 0.437
2.581GlyAsn: 2.581 ± 0.502
2.737GlyPro: 2.737 ± 0.529
3.676GlyGln: 3.676 ± 0.699
5.24GlyArg: 5.24 ± 0.584
4.693GlySer: 4.693 ± 0.578
4.536GlyThr: 4.536 ± 0.815
5.005GlyVal: 5.005 ± 0.756
1.564GlyTrp: 1.564 ± 0.375
2.816GlyTyr: 2.816 ± 0.471
0.0GlyXaa: 0.0 ± 0.0
His
1.251HisAla: 1.251 ± 0.309
0.313HisCys: 0.313 ± 0.12
1.017HisAsp: 1.017 ± 0.276
1.564HisGlu: 1.564 ± 0.388
0.704HisPhe: 0.704 ± 0.274
0.547HisGly: 0.547 ± 0.209
0.547HisHis: 0.547 ± 0.294
1.017HisIle: 1.017 ± 0.277
0.86HisLys: 0.86 ± 0.302
1.408HisLeu: 1.408 ± 0.33
0.469HisMet: 0.469 ± 0.211
0.626HisAsn: 0.626 ± 0.206
1.173HisPro: 1.173 ± 0.326
0.626HisGln: 0.626 ± 0.262
0.939HisArg: 0.939 ± 0.331
0.704HisSer: 0.704 ± 0.191
1.017HisThr: 1.017 ± 0.341
0.782HisVal: 0.782 ± 0.248
0.469HisTrp: 0.469 ± 0.168
0.469HisTyr: 0.469 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
5.631IleAla: 5.631 ± 0.689
0.547IleCys: 0.547 ± 0.203
3.754IleAsp: 3.754 ± 0.49
4.145IleGlu: 4.145 ± 0.487
1.251IlePhe: 1.251 ± 0.341
3.05IleGly: 3.05 ± 0.561
0.704IleHis: 0.704 ± 0.25
2.503IleIle: 2.503 ± 0.667
2.581IleLys: 2.581 ± 0.41
3.441IleLeu: 3.441 ± 0.667
1.799IleMet: 1.799 ± 0.346
1.642IleAsn: 1.642 ± 0.358
3.05IlePro: 3.05 ± 0.485
2.112IleGln: 2.112 ± 0.455
3.285IleArg: 3.285 ± 0.646
2.346IleSer: 2.346 ± 0.434
2.894IleThr: 2.894 ± 0.472
3.441IleVal: 3.441 ± 0.645
0.704IleTrp: 0.704 ± 0.232
1.564IleTyr: 1.564 ± 0.373
0.0IleXaa: 0.0 ± 0.0
Lys
6.491LysAla: 6.491 ± 0.701
0.469LysCys: 0.469 ± 0.207
4.302LysAsp: 4.302 ± 0.556
4.302LysGlu: 4.302 ± 0.684
1.173LysPhe: 1.173 ± 0.258
3.519LysGly: 3.519 ± 0.518
1.251LysHis: 1.251 ± 0.306
2.503LysIle: 2.503 ± 0.457
4.145LysLys: 4.145 ± 0.667
4.223LysLeu: 4.223 ± 0.554
2.033LysMet: 2.033 ± 0.354
2.19LysAsn: 2.19 ± 0.38
2.425LysPro: 2.425 ± 0.38
2.894LysGln: 2.894 ± 0.47
3.207LysArg: 3.207 ± 0.52
2.346LysSer: 2.346 ± 0.416
2.503LysThr: 2.503 ± 0.463
2.894LysVal: 2.894 ± 0.437
0.626LysTrp: 0.626 ± 0.198
2.033LysTyr: 2.033 ± 0.539
0.0LysXaa: 0.0 ± 0.0
Leu
9.072LeuAla: 9.072 ± 0.834
0.782LeuCys: 0.782 ± 0.233
5.162LeuAsp: 5.162 ± 0.6
4.38LeuGlu: 4.38 ± 0.687
2.19LeuPhe: 2.19 ± 0.305
5.24LeuGly: 5.24 ± 0.907
1.095LeuHis: 1.095 ± 0.261
3.832LeuIle: 3.832 ± 0.464
3.598LeuLys: 3.598 ± 0.537
5.866LeuLeu: 5.866 ± 0.806
2.112LeuMet: 2.112 ± 0.437
3.676LeuAsn: 3.676 ± 0.555
4.145LeuPro: 4.145 ± 0.583
2.346LeuGln: 2.346 ± 0.512
4.536LeuArg: 4.536 ± 0.747
3.911LeuSer: 3.911 ± 0.523
5.084LeuThr: 5.084 ± 0.6
5.788LeuVal: 5.788 ± 0.6
1.017LeuTrp: 1.017 ± 0.301
2.112LeuTyr: 2.112 ± 0.413
0.0LeuXaa: 0.0 ± 0.0
Met
4.223MetAla: 4.223 ± 0.511
0.313MetCys: 0.313 ± 0.153
1.721MetAsp: 1.721 ± 0.53
1.877MetGlu: 1.877 ± 0.387
1.251MetPhe: 1.251 ± 0.363
1.173MetGly: 1.173 ± 0.292
0.156MetHis: 0.156 ± 0.12
2.268MetIle: 2.268 ± 0.396
1.251MetLys: 1.251 ± 0.306
1.173MetLeu: 1.173 ± 0.247
0.939MetMet: 0.939 ± 0.257
1.173MetAsn: 1.173 ± 0.22
1.564MetPro: 1.564 ± 0.338
1.408MetGln: 1.408 ± 0.289
1.721MetArg: 1.721 ± 0.438
1.408MetSer: 1.408 ± 0.354
1.564MetThr: 1.564 ± 0.309
1.642MetVal: 1.642 ± 0.505
0.235MetTrp: 0.235 ± 0.128
0.704MetTyr: 0.704 ± 0.234
0.0MetXaa: 0.0 ± 0.0
Asn
5.084AsnAla: 5.084 ± 0.589
0.156AsnCys: 0.156 ± 0.123
2.033AsnAsp: 2.033 ± 0.366
1.486AsnGlu: 1.486 ± 0.346
0.939AsnPhe: 0.939 ± 0.278
4.693AsnGly: 4.693 ± 0.544
0.86AsnHis: 0.86 ± 0.278
2.19AsnIle: 2.19 ± 0.479
2.581AsnLys: 2.581 ± 0.365
3.128AsnLeu: 3.128 ± 0.52
1.33AsnMet: 1.33 ± 0.297
1.408AsnAsn: 1.408 ± 0.389
2.816AsnPro: 2.816 ± 0.526
1.799AsnGln: 1.799 ± 0.43
2.112AsnArg: 2.112 ± 0.409
2.268AsnSer: 2.268 ± 0.39
1.955AsnThr: 1.955 ± 0.409
2.894AsnVal: 2.894 ± 0.586
0.86AsnTrp: 0.86 ± 0.247
0.86AsnTyr: 0.86 ± 0.233
0.0AsnXaa: 0.0 ± 0.0
Pro
5.788ProAla: 5.788 ± 0.807
0.547ProCys: 0.547 ± 0.238
4.614ProAsp: 4.614 ± 0.51
4.223ProGlu: 4.223 ± 0.583
1.799ProPhe: 1.799 ± 0.358
3.989ProGly: 3.989 ± 0.784
0.704ProHis: 0.704 ± 0.251
1.955ProIle: 1.955 ± 0.343
1.955ProLys: 1.955 ± 0.37
3.128ProLeu: 3.128 ± 0.638
0.704ProMet: 0.704 ± 0.23
1.486ProAsn: 1.486 ± 0.332
1.877ProPro: 1.877 ± 0.509
1.877ProGln: 1.877 ± 0.509
1.955ProArg: 1.955 ± 0.459
2.19ProSer: 2.19 ± 0.401
3.989ProThr: 3.989 ± 0.542
2.816ProVal: 2.816 ± 0.438
0.547ProTrp: 0.547 ± 0.183
1.799ProTyr: 1.799 ± 0.467
0.0ProXaa: 0.0 ± 0.0
Gln
5.944GlnAla: 5.944 ± 1.236
0.235GlnCys: 0.235 ± 0.2
2.581GlnAsp: 2.581 ± 0.427
2.972GlnGlu: 2.972 ± 0.562
1.564GlnPhe: 1.564 ± 0.344
2.659GlnGly: 2.659 ± 0.613
0.547GlnHis: 0.547 ± 0.232
2.033GlnIle: 2.033 ± 0.452
2.894GlnLys: 2.894 ± 0.509
3.754GlnLeu: 3.754 ± 0.621
1.408GlnMet: 1.408 ± 0.322
2.346GlnAsn: 2.346 ± 0.401
1.486GlnPro: 1.486 ± 0.324
5.162GlnGln: 5.162 ± 0.981
2.816GlnArg: 2.816 ± 0.59
1.721GlnSer: 1.721 ± 0.386
2.425GlnThr: 2.425 ± 0.425
2.425GlnVal: 2.425 ± 0.349
0.782GlnTrp: 0.782 ± 0.2
1.095GlnTyr: 1.095 ± 0.364
0.0GlnXaa: 0.0 ± 0.0
Arg
5.631ArgAla: 5.631 ± 0.595
0.704ArgCys: 0.704 ± 0.31
3.989ArgAsp: 3.989 ± 0.522
4.302ArgGlu: 4.302 ± 0.681
2.425ArgPhe: 2.425 ± 0.533
4.145ArgGly: 4.145 ± 0.531
0.86ArgHis: 0.86 ± 0.237
3.519ArgIle: 3.519 ± 0.683
4.145ArgLys: 4.145 ± 0.731
4.849ArgLeu: 4.849 ± 0.636
1.564ArgMet: 1.564 ± 0.378
3.363ArgAsn: 3.363 ± 0.597
2.346ArgPro: 2.346 ± 0.45
2.972ArgGln: 2.972 ± 0.411
3.441ArgArg: 3.441 ± 0.601
3.285ArgSer: 3.285 ± 0.479
2.894ArgThr: 2.894 ± 0.421
3.832ArgVal: 3.832 ± 0.65
0.782ArgTrp: 0.782 ± 0.271
2.033ArgTyr: 2.033 ± 0.412
0.0ArgXaa: 0.0 ± 0.0
Ser
4.38SerAla: 4.38 ± 0.699
0.235SerCys: 0.235 ± 0.127
3.363SerAsp: 3.363 ± 0.557
2.581SerGlu: 2.581 ± 0.457
1.799SerPhe: 1.799 ± 0.385
5.005SerGly: 5.005 ± 0.871
0.704SerHis: 0.704 ± 0.351
1.799SerIle: 1.799 ± 0.315
3.05SerLys: 3.05 ± 0.427
3.676SerLeu: 3.676 ± 0.546
1.173SerMet: 1.173 ± 0.275
2.503SerAsn: 2.503 ± 0.503
2.268SerPro: 2.268 ± 0.347
2.033SerGln: 2.033 ± 0.396
3.441SerArg: 3.441 ± 0.517
3.207SerSer: 3.207 ± 1.048
3.519SerThr: 3.519 ± 0.528
3.676SerVal: 3.676 ± 0.491
0.469SerTrp: 0.469 ± 0.2
1.408SerTyr: 1.408 ± 0.359
0.0SerXaa: 0.0 ± 0.0
Thr
5.709ThrAla: 5.709 ± 0.905
0.469ThrCys: 0.469 ± 0.202
4.067ThrAsp: 4.067 ± 0.571
3.441ThrGlu: 3.441 ± 0.627
1.33ThrPhe: 1.33 ± 0.306
5.709ThrGly: 5.709 ± 0.6
0.86ThrHis: 0.86 ± 0.267
2.659ThrIle: 2.659 ± 0.451
2.816ThrLys: 2.816 ± 0.5
4.38ThrLeu: 4.38 ± 0.589
1.251ThrMet: 1.251 ± 0.246
2.268ThrAsn: 2.268 ± 0.51
4.302ThrPro: 4.302 ± 0.728
2.346ThrGln: 2.346 ± 0.595
2.816ThrArg: 2.816 ± 0.505
2.816ThrSer: 2.816 ± 0.528
2.659ThrThr: 2.659 ± 0.563
3.598ThrVal: 3.598 ± 0.55
0.704ThrTrp: 0.704 ± 0.243
1.564ThrTyr: 1.564 ± 0.26
0.0ThrXaa: 0.0 ± 0.0
Val
6.804ValAla: 6.804 ± 0.752
1.095ValCys: 1.095 ± 0.325
3.363ValAsp: 3.363 ± 0.574
4.067ValGlu: 4.067 ± 0.552
2.033ValPhe: 2.033 ± 0.429
4.614ValGly: 4.614 ± 0.514
0.939ValHis: 0.939 ± 0.227
3.363ValIle: 3.363 ± 0.455
3.989ValLys: 3.989 ± 0.597
3.989ValLeu: 3.989 ± 0.526
1.877ValMet: 1.877 ± 0.323
2.581ValAsn: 2.581 ± 0.518
2.972ValPro: 2.972 ± 0.512
1.721ValGln: 1.721 ± 0.426
3.832ValArg: 3.832 ± 0.638
2.894ValSer: 2.894 ± 0.51
3.598ValThr: 3.598 ± 0.667
3.285ValVal: 3.285 ± 0.503
1.095ValTrp: 1.095 ± 0.372
1.877ValTyr: 1.877 ± 0.431
0.0ValXaa: 0.0 ± 0.0
Trp
0.86TrpAla: 0.86 ± 0.227
0.313TrpCys: 0.313 ± 0.148
1.251TrpAsp: 1.251 ± 0.299
1.408TrpGlu: 1.408 ± 0.32
0.86TrpPhe: 0.86 ± 0.278
1.486TrpGly: 1.486 ± 0.301
0.547TrpHis: 0.547 ± 0.236
1.017TrpIle: 1.017 ± 0.256
0.547TrpLys: 0.547 ± 0.177
1.486TrpLeu: 1.486 ± 0.36
0.391TrpMet: 0.391 ± 0.233
1.095TrpAsn: 1.095 ± 0.273
0.547TrpPro: 0.547 ± 0.188
0.782TrpGln: 0.782 ± 0.285
1.173TrpArg: 1.173 ± 0.266
0.704TrpSer: 0.704 ± 0.257
1.251TrpThr: 1.251 ± 0.323
0.547TrpVal: 0.547 ± 0.211
0.156TrpTrp: 0.156 ± 0.124
0.156TrpTyr: 0.156 ± 0.104
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.441TyrAla: 3.441 ± 0.491
0.469TyrCys: 0.469 ± 0.221
1.955TyrAsp: 1.955 ± 0.432
1.877TyrGlu: 1.877 ± 0.425
1.017TyrPhe: 1.017 ± 0.289
2.346TyrGly: 2.346 ± 0.468
0.704TyrHis: 0.704 ± 0.241
0.86TyrIle: 0.86 ± 0.237
0.86TyrLys: 0.86 ± 0.266
3.05TyrLeu: 3.05 ± 0.47
0.86TyrMet: 0.86 ± 0.265
1.173TyrAsn: 1.173 ± 0.348
1.095TyrPro: 1.095 ± 0.429
1.017TyrGln: 1.017 ± 0.201
1.33TyrArg: 1.33 ± 0.329
1.799TyrSer: 1.799 ± 0.461
1.877TyrThr: 1.877 ± 0.311
1.955TyrVal: 1.955 ± 0.299
0.235TyrTrp: 0.235 ± 0.15
1.017TyrTyr: 1.017 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (12787 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski