Wuhan insect virus 18
Average proteome isoelectric point is 9.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KI68|A0A1L3KI68_9VIRU RNA-dependent RNA polymerase OS=Wuhan insect virus 18 OX=1923722 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 9.89 PPRR5 pKa = 11.84 PTEE8 pKa = 3.66 VHH10 pKa = 6.2 KK11 pKa = 10.81 DD12 pKa = 3.26 FRR14 pKa = 11.84 LPVLLRR20 pKa = 11.84 ACRR23 pKa = 11.84 VGGTWSRR30 pKa = 11.84 DD31 pKa = 2.95 VSRR34 pKa = 11.84 YY35 pKa = 8.93 LVPALQDD42 pKa = 3.51 GVGVRR47 pKa = 11.84 PGEE50 pKa = 4.27 GGNPQTRR57 pKa = 11.84 PRR59 pKa = 11.84 KK60 pKa = 7.74 PAKK63 pKa = 10.39 SVDD66 pKa = 3.4 LEE68 pKa = 3.88 RR69 pKa = 11.84 TRR71 pKa = 11.84 RR72 pKa = 11.84 YY73 pKa = 10.75 DD74 pKa = 3.15 LTVRR78 pKa = 11.84 WRR80 pKa = 11.84 KK81 pKa = 7.58 EE82 pKa = 3.33 FRR84 pKa = 11.84 SRR86 pKa = 11.84 GRR88 pKa = 11.84 LVEE91 pKa = 4.21 GAWAAVHH98 pKa = 6.02 AALVAAHH105 pKa = 7.42 PDD107 pKa = 3.18 LGRR110 pKa = 11.84 ADD112 pKa = 3.3 MSTRR116 pKa = 11.84 AGRR119 pKa = 11.84 DD120 pKa = 3.49 RR121 pKa = 11.84 IWAWRR126 pKa = 11.84 RR127 pKa = 11.84 LAVWMVRR134 pKa = 11.84 WIDD137 pKa = 3.4 RR138 pKa = 11.84 DD139 pKa = 3.46 PRR141 pKa = 11.84 DD142 pKa = 3.48 MVRR145 pKa = 11.84 GWKK148 pKa = 9.32 QLAATFRR155 pKa = 11.84 EE156 pKa = 4.02 ASIMQKK162 pKa = 10.37 PYY164 pKa = 10.96 DD165 pKa = 3.73 WAAAPAGFPKK175 pKa = 10.45 RR176 pKa = 11.84 LRR178 pKa = 11.84 PVLAGVRR185 pKa = 11.84 LTPPVLDD192 pKa = 3.26 QLSYY196 pKa = 10.56 IGRR199 pKa = 11.84 ALPQGDD205 pKa = 3.0 AWVQRR210 pKa = 11.84 KK211 pKa = 8.86 ALDD214 pKa = 3.45 DD215 pKa = 3.75 HH216 pKa = 7.12 RR217 pKa = 11.84 EE218 pKa = 4.21 CLSSAPRR225 pKa = 11.84 PLANHH230 pKa = 5.97 VLAAARR236 pKa = 11.84 DD237 pKa = 4.26 FARR240 pKa = 11.84 SWASRR245 pKa = 11.84 HH246 pKa = 4.66 VRR248 pKa = 11.84 PSGRR252 pKa = 11.84 ISHH255 pKa = 6.39 PKK257 pKa = 9.05 VLEE260 pKa = 3.96 SATIQEE266 pKa = 4.35 PRR268 pKa = 11.84 RR269 pKa = 11.84 SGGVAAHH276 pKa = 6.99 LCKK279 pKa = 10.27 QGFDD283 pKa = 3.74 LPPFEE288 pKa = 5.26 GLTDD292 pKa = 3.63 EE293 pKa = 5.81 LRR295 pKa = 11.84 DD296 pKa = 3.54 QIRR299 pKa = 11.84 AMGEE303 pKa = 4.17 SAPSDD308 pKa = 3.69 CDD310 pKa = 3.35 LEE312 pKa = 6.01 AIGRR316 pKa = 11.84 STAFRR321 pKa = 11.84 DD322 pKa = 3.19 WALGQLCCPDD332 pKa = 3.7 ASPPRR337 pKa = 11.84 SRR339 pKa = 11.84 VEE341 pKa = 4.28 AIPEE345 pKa = 3.94 RR346 pKa = 11.84 GFKK349 pKa = 10.66 ARR351 pKa = 11.84 VVTVPDD357 pKa = 3.81 LPEE360 pKa = 5.79 SILGSALRR368 pKa = 11.84 CYY370 pKa = 10.58 LLPALRR376 pKa = 11.84 RR377 pKa = 11.84 TPEE380 pKa = 4.13 CADD383 pKa = 3.28 VLSGKK388 pKa = 10.18 EE389 pKa = 3.59 EE390 pKa = 3.86 LAAMRR395 pKa = 11.84 IASSRR400 pKa = 11.84 RR401 pKa = 11.84 PIEE404 pKa = 4.07 GDD406 pKa = 3.3 WIVSSDD412 pKa = 3.4 LTAASDD418 pKa = 3.68 TLSFEE423 pKa = 4.45 LLQALVDD430 pKa = 3.93 GLEE433 pKa = 4.27 EE434 pKa = 5.0 SGTLPPWAIRR444 pKa = 11.84 ALKK447 pKa = 10.37 VLVGPQEE454 pKa = 4.02 LVYY457 pKa = 10.46 PDD459 pKa = 3.89 GSVLVTRR466 pKa = 11.84 RR467 pKa = 11.84 GTLMGLGHH475 pKa = 6.62 TWSILSLSHH484 pKa = 7.08 LFWLDD489 pKa = 2.9 WAHH492 pKa = 6.88 RR493 pKa = 11.84 SVDD496 pKa = 3.37 PRR498 pKa = 11.84 VADD501 pKa = 3.67 VVRR504 pKa = 11.84 RR505 pKa = 11.84 TAAVCGDD512 pKa = 4.16 DD513 pKa = 3.82 LVVRR517 pKa = 11.84 APLKK521 pKa = 9.49 WVQSYY526 pKa = 9.39 HH527 pKa = 6.19 QIAEE531 pKa = 3.9 ASGARR536 pKa = 11.84 FSTGKK541 pKa = 9.97 HH542 pKa = 3.87 FVAKK546 pKa = 9.88 RR547 pKa = 11.84 RR548 pKa = 11.84 YY549 pKa = 9.33 VFLEE553 pKa = 4.48 RR554 pKa = 11.84 IWSLKK559 pKa = 10.54 SSLQRR564 pKa = 11.84 VRR566 pKa = 11.84 LPRR569 pKa = 11.84 EE570 pKa = 3.56 RR571 pKa = 11.84 TRR573 pKa = 11.84 RR574 pKa = 11.84 AGRR577 pKa = 11.84 WVRR580 pKa = 11.84 EE581 pKa = 3.96 FPPLGARR588 pKa = 11.84 PVKK591 pKa = 10.28 VRR593 pKa = 11.84 PTVVRR598 pKa = 11.84 SIPSRR603 pKa = 11.84 SVPSTAIPLAGLTAGEE619 pKa = 4.41 VDD621 pKa = 3.86 CHH623 pKa = 6.69 FGPVRR628 pKa = 11.84 NLVLPNWVRR637 pKa = 11.84 LGMAVEE643 pKa = 4.41 TLLHH647 pKa = 6.04 SPEE650 pKa = 3.86 AARR653 pKa = 11.84 KK654 pKa = 7.5 VRR656 pKa = 11.84 KK657 pKa = 9.0 VVRR660 pKa = 11.84 ALYY663 pKa = 9.63 PGLPRR668 pKa = 11.84 WMRR671 pKa = 11.84 RR672 pKa = 11.84 HH673 pKa = 5.88 GVQPYY678 pKa = 10.23 LPRR681 pKa = 11.84 FLGGGGLLPPGRR693 pKa = 11.84 NVTRR697 pKa = 11.84 IGRR700 pKa = 11.84 LPRR703 pKa = 11.84 PVAKK707 pKa = 10.35 AVTTWVYY714 pKa = 11.0 GRR716 pKa = 11.84 SPRR719 pKa = 11.84 EE720 pKa = 3.7 NLSLAKK726 pKa = 9.94 IYY728 pKa = 10.89 LPTTRR733 pKa = 11.84 RR734 pKa = 11.84 ITNYY738 pKa = 10.54 AVGEE742 pKa = 3.93 QHH744 pKa = 5.78 TAVRR748 pKa = 11.84 FQQVSAVLCRR758 pKa = 11.84 AGKK761 pKa = 10.41 KK762 pKa = 10.19 PFDD765 pKa = 3.3 GHH767 pKa = 5.1 GRR769 pKa = 11.84 RR770 pKa = 11.84 LGTLSEE776 pKa = 4.26 APEE779 pKa = 3.98 AAALAIARR787 pKa = 11.84 EE788 pKa = 4.18 LSMVLLPEE796 pKa = 4.38 PSEE799 pKa = 3.71 EE800 pKa = 3.62 RR801 pKa = 11.84 AYY803 pKa = 10.85 RR804 pKa = 11.84 RR805 pKa = 11.84 LLRR808 pKa = 11.84 PSQVGQTFRR817 pKa = 11.84 RR818 pKa = 11.84 LVLRR822 pKa = 11.84 ANRR825 pKa = 11.84 RR826 pKa = 11.84 WPGSKK831 pKa = 9.67 PICPRR836 pKa = 11.84 APRR839 pKa = 11.84 RR840 pKa = 11.84 ALLARR845 pKa = 11.84 CKK847 pKa = 10.11 AVEE850 pKa = 4.22 EE851 pKa = 4.82 GFVWWANPRR860 pKa = 11.84 LLTQFGFGGWGLEE873 pKa = 3.98 PAAQRR878 pKa = 11.84 VVKK881 pKa = 10.53 DD882 pKa = 3.36 ALGWSTFRR890 pKa = 11.84 PTPGRR895 pKa = 11.84 GTHH898 pKa = 6.02
Molecular weight: 100.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.253
IPC2_protein 9.765
IPC_protein 11.023
Toseland 11.155
ProMoST 11.506
Dawson 11.184
Bjellqvist 11.096
Wikipedia 11.579
Rodwell 11.067
Grimsley 11.228
Solomon 11.579
Lehninger 11.491
Nozaki 11.155
DTASelect 11.096
Thurlkill 11.155
EMBOSS 11.623
Sillero 11.169
Patrickios 10.76
IPC_peptide 11.579
IPC2_peptide 10.526
IPC2.peptide.svr19 9.258
Protein with the highest isoelectric point:
>tr|A0A1L3KI68|A0A1L3KI68_9VIRU RNA-dependent RNA polymerase OS=Wuhan insect virus 18 OX=1923722 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 9.89 PPRR5 pKa = 11.84 PTEE8 pKa = 3.66 VHH10 pKa = 6.2 KK11 pKa = 10.81 DD12 pKa = 3.26 FRR14 pKa = 11.84 LPVLLRR20 pKa = 11.84 ACRR23 pKa = 11.84 VGGTWSRR30 pKa = 11.84 DD31 pKa = 2.95 VSRR34 pKa = 11.84 YY35 pKa = 8.93 LVPALQDD42 pKa = 3.51 GVGVRR47 pKa = 11.84 PGEE50 pKa = 4.27 GGNPQTRR57 pKa = 11.84 PRR59 pKa = 11.84 KK60 pKa = 7.74 PAKK63 pKa = 10.39 SVDD66 pKa = 3.4 LEE68 pKa = 3.88 RR69 pKa = 11.84 TRR71 pKa = 11.84 RR72 pKa = 11.84 YY73 pKa = 10.75 DD74 pKa = 3.15 LTVRR78 pKa = 11.84 WRR80 pKa = 11.84 KK81 pKa = 7.58 EE82 pKa = 3.33 FRR84 pKa = 11.84 SRR86 pKa = 11.84 GRR88 pKa = 11.84 LVEE91 pKa = 4.21 GAWAAVHH98 pKa = 6.02 AALVAAHH105 pKa = 7.42 PDD107 pKa = 3.18 LGRR110 pKa = 11.84 ADD112 pKa = 3.3 MSTRR116 pKa = 11.84 AGRR119 pKa = 11.84 DD120 pKa = 3.49 RR121 pKa = 11.84 IWAWRR126 pKa = 11.84 RR127 pKa = 11.84 LAVWMVRR134 pKa = 11.84 WIDD137 pKa = 3.4 RR138 pKa = 11.84 DD139 pKa = 3.46 PRR141 pKa = 11.84 DD142 pKa = 3.48 MVRR145 pKa = 11.84 GWKK148 pKa = 9.32 QLAATFRR155 pKa = 11.84 EE156 pKa = 4.02 ASIMQKK162 pKa = 10.37 PYY164 pKa = 10.96 DD165 pKa = 3.73 WAAAPAGFPKK175 pKa = 10.45 RR176 pKa = 11.84 LRR178 pKa = 11.84 PVLAGVRR185 pKa = 11.84 LTPPVLDD192 pKa = 3.26 QLSYY196 pKa = 10.56 IGRR199 pKa = 11.84 ALPQGDD205 pKa = 3.0 AWVQRR210 pKa = 11.84 KK211 pKa = 8.86 ALDD214 pKa = 3.45 DD215 pKa = 3.75 HH216 pKa = 7.12 RR217 pKa = 11.84 EE218 pKa = 4.21 CLSSAPRR225 pKa = 11.84 PLANHH230 pKa = 5.97 VLAAARR236 pKa = 11.84 DD237 pKa = 4.26 FARR240 pKa = 11.84 SWASRR245 pKa = 11.84 HH246 pKa = 4.66 VRR248 pKa = 11.84 PSGRR252 pKa = 11.84 ISHH255 pKa = 6.39 PKK257 pKa = 9.05 VLEE260 pKa = 3.96 SATIQEE266 pKa = 4.35 PRR268 pKa = 11.84 RR269 pKa = 11.84 SGGVAAHH276 pKa = 6.99 LCKK279 pKa = 10.27 QGFDD283 pKa = 3.74 LPPFEE288 pKa = 5.26 GLTDD292 pKa = 3.63 EE293 pKa = 5.81 LRR295 pKa = 11.84 DD296 pKa = 3.54 QIRR299 pKa = 11.84 AMGEE303 pKa = 4.17 SAPSDD308 pKa = 3.69 CDD310 pKa = 3.35 LEE312 pKa = 6.01 AIGRR316 pKa = 11.84 STAFRR321 pKa = 11.84 DD322 pKa = 3.19 WALGQLCCPDD332 pKa = 3.7 ASPPRR337 pKa = 11.84 SRR339 pKa = 11.84 VEE341 pKa = 4.28 AIPEE345 pKa = 3.94 RR346 pKa = 11.84 GFKK349 pKa = 10.66 ARR351 pKa = 11.84 VVTVPDD357 pKa = 3.81 LPEE360 pKa = 5.79 SILGSALRR368 pKa = 11.84 CYY370 pKa = 10.58 LLPALRR376 pKa = 11.84 RR377 pKa = 11.84 TPEE380 pKa = 4.13 CADD383 pKa = 3.28 VLSGKK388 pKa = 10.18 EE389 pKa = 3.59 EE390 pKa = 3.86 LAAMRR395 pKa = 11.84 IASSRR400 pKa = 11.84 RR401 pKa = 11.84 PIEE404 pKa = 4.07 GDD406 pKa = 3.3 WIVSSDD412 pKa = 3.4 LTAASDD418 pKa = 3.68 TLSFEE423 pKa = 4.45 LLQALVDD430 pKa = 3.93 GLEE433 pKa = 4.27 EE434 pKa = 5.0 SGTLPPWAIRR444 pKa = 11.84 ALKK447 pKa = 10.37 VLVGPQEE454 pKa = 4.02 LVYY457 pKa = 10.46 PDD459 pKa = 3.89 GSVLVTRR466 pKa = 11.84 RR467 pKa = 11.84 GTLMGLGHH475 pKa = 6.62 TWSILSLSHH484 pKa = 7.08 LFWLDD489 pKa = 2.9 WAHH492 pKa = 6.88 RR493 pKa = 11.84 SVDD496 pKa = 3.37 PRR498 pKa = 11.84 VADD501 pKa = 3.67 VVRR504 pKa = 11.84 RR505 pKa = 11.84 TAAVCGDD512 pKa = 4.16 DD513 pKa = 3.82 LVVRR517 pKa = 11.84 APLKK521 pKa = 9.49 WVQSYY526 pKa = 9.39 HH527 pKa = 6.19 QIAEE531 pKa = 3.9 ASGARR536 pKa = 11.84 FSTGKK541 pKa = 9.97 HH542 pKa = 3.87 FVAKK546 pKa = 9.88 RR547 pKa = 11.84 RR548 pKa = 11.84 YY549 pKa = 9.33 VFLEE553 pKa = 4.48 RR554 pKa = 11.84 IWSLKK559 pKa = 10.54 SSLQRR564 pKa = 11.84 VRR566 pKa = 11.84 LPRR569 pKa = 11.84 EE570 pKa = 3.56 RR571 pKa = 11.84 TRR573 pKa = 11.84 RR574 pKa = 11.84 AGRR577 pKa = 11.84 WVRR580 pKa = 11.84 EE581 pKa = 3.96 FPPLGARR588 pKa = 11.84 PVKK591 pKa = 10.28 VRR593 pKa = 11.84 PTVVRR598 pKa = 11.84 SIPSRR603 pKa = 11.84 SVPSTAIPLAGLTAGEE619 pKa = 4.41 VDD621 pKa = 3.86 CHH623 pKa = 6.69 FGPVRR628 pKa = 11.84 NLVLPNWVRR637 pKa = 11.84 LGMAVEE643 pKa = 4.41 TLLHH647 pKa = 6.04 SPEE650 pKa = 3.86 AARR653 pKa = 11.84 KK654 pKa = 7.5 VRR656 pKa = 11.84 KK657 pKa = 9.0 VVRR660 pKa = 11.84 ALYY663 pKa = 9.63 PGLPRR668 pKa = 11.84 WMRR671 pKa = 11.84 RR672 pKa = 11.84 HH673 pKa = 5.88 GVQPYY678 pKa = 10.23 LPRR681 pKa = 11.84 FLGGGGLLPPGRR693 pKa = 11.84 NVTRR697 pKa = 11.84 IGRR700 pKa = 11.84 LPRR703 pKa = 11.84 PVAKK707 pKa = 10.35 AVTTWVYY714 pKa = 11.0 GRR716 pKa = 11.84 SPRR719 pKa = 11.84 EE720 pKa = 3.7 NLSLAKK726 pKa = 9.94 IYY728 pKa = 10.89 LPTTRR733 pKa = 11.84 RR734 pKa = 11.84 ITNYY738 pKa = 10.54 AVGEE742 pKa = 3.93 QHH744 pKa = 5.78 TAVRR748 pKa = 11.84 FQQVSAVLCRR758 pKa = 11.84 AGKK761 pKa = 10.41 KK762 pKa = 10.19 PFDD765 pKa = 3.3 GHH767 pKa = 5.1 GRR769 pKa = 11.84 RR770 pKa = 11.84 LGTLSEE776 pKa = 4.26 APEE779 pKa = 3.98 AAALAIARR787 pKa = 11.84 EE788 pKa = 4.18 LSMVLLPEE796 pKa = 4.38 PSEE799 pKa = 3.71 EE800 pKa = 3.62 RR801 pKa = 11.84 AYY803 pKa = 10.85 RR804 pKa = 11.84 RR805 pKa = 11.84 LLRR808 pKa = 11.84 PSQVGQTFRR817 pKa = 11.84 RR818 pKa = 11.84 LVLRR822 pKa = 11.84 ANRR825 pKa = 11.84 RR826 pKa = 11.84 WPGSKK831 pKa = 9.67 PICPRR836 pKa = 11.84 APRR839 pKa = 11.84 RR840 pKa = 11.84 ALLARR845 pKa = 11.84 CKK847 pKa = 10.11 AVEE850 pKa = 4.22 EE851 pKa = 4.82 GFVWWANPRR860 pKa = 11.84 LLTQFGFGGWGLEE873 pKa = 3.98 PAAQRR878 pKa = 11.84 VVKK881 pKa = 10.53 DD882 pKa = 3.36 ALGWSTFRR890 pKa = 11.84 PTPGRR895 pKa = 11.84 GTHH898 pKa = 6.02
Molecular weight: 100.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.253
IPC2_protein 9.765
IPC_protein 11.023
Toseland 11.155
ProMoST 11.506
Dawson 11.184
Bjellqvist 11.096
Wikipedia 11.579
Rodwell 11.067
Grimsley 11.228
Solomon 11.579
Lehninger 11.491
Nozaki 11.155
DTASelect 11.096
Thurlkill 11.155
EMBOSS 11.623
Sillero 11.169
Patrickios 10.76
IPC_peptide 11.579
IPC2_peptide 10.526
IPC2.peptide.svr19 9.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
898
898
898
898.0
100.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.579 ± 0.0
1.448 ± 0.0
4.343 ± 0.0
4.677 ± 0.0
2.673 ± 0.0
7.572 ± 0.0
2.116 ± 0.0
2.673 ± 0.0
3.118 ± 0.0
10.69 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.225 ± 0.0
1.002 ± 0.0
8.352 ± 0.0
2.673 ± 0.0
13.252 ± 0.0
6.125 ± 0.0
4.343 ± 0.0
8.463 ± 0.0
3.118 ± 0.0
1.559 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here