Ostreococcus tauri virus 1
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 230 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7U0D1|C7U0D1_9PHYC Uncharacterized protein OS=Ostreococcus tauri virus 1 OX=642926 GN=OTV1_099 PE=4 SV=1
MM1 pKa = 7.28 MKK3 pKa = 9.75 TGFGEE8 pKa = 4.13 SSGDD12 pKa = 3.65 YY13 pKa = 9.7 EE14 pKa = 5.51 ASQQQALIGILLPVLEE30 pKa = 5.03 KK31 pKa = 11.13 SMVMAAEE38 pKa = 4.06 YY39 pKa = 10.97 SKK41 pKa = 11.45 ACGRR45 pKa = 11.84 NTVLPEE51 pKa = 4.14 DD52 pKa = 3.44 MEE54 pKa = 4.46 YY55 pKa = 10.7 AIKK58 pKa = 10.22 YY59 pKa = 9.78 CVMHH63 pKa = 6.14 TVGQNIGSLFPEE75 pKa = 4.91 IYY77 pKa = 10.35 DD78 pKa = 3.76 EE79 pKa = 4.39 EE80 pKa = 5.69 SSDD83 pKa = 3.81 EE84 pKa = 4.28 EE85 pKa = 4.21 EE86 pKa = 4.79 LEE88 pKa = 4.13 EE89 pKa = 5.47 VPPEE93 pKa = 4.16 DD94 pKa = 3.83 CPPFEE99 pKa = 5.8 RR100 pKa = 11.84 YY101 pKa = 8.11 TGDD104 pKa = 3.2 DD105 pKa = 3.23 TTFRR109 pKa = 11.84 QMNEE113 pKa = 3.65 AYY115 pKa = 10.1 DD116 pKa = 4.02 RR117 pKa = 11.84 WEE119 pKa = 3.58 QWVPQSPVEE128 pKa = 4.04 EE129 pKa = 4.05 MLKK132 pKa = 10.56 NAINSNEE139 pKa = 4.05 YY140 pKa = 9.9 IGSGGLDD147 pKa = 3.37 DD148 pKa = 5.04 FF149 pKa = 5.68
Molecular weight: 16.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.973
IPC_protein 3.897
Toseland 3.732
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.732
Grimsley 3.643
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.088
Thurlkill 3.745
EMBOSS 3.732
Sillero 4.012
Patrickios 1.036
IPC_peptide 3.846
IPC2_peptide 3.999
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|C7U0F0|C7U0F0_9PHYC Uncharacterized protein OS=Ostreococcus tauri virus 1 OX=642926 GN=OTV1_118 PE=4 SV=1
MM1 pKa = 7.43 LPDD4 pKa = 3.11 ITSQKK9 pKa = 9.58 VAIPAALFLALSPGVLLTTDD29 pKa = 3.08 GRR31 pKa = 11.84 RR32 pKa = 11.84 VNFVNGKK39 pKa = 6.16 TSQMAVMFHH48 pKa = 6.59 ALVFFLVYY56 pKa = 10.7 SLVAKK61 pKa = 10.91 AMGLVLTKK69 pKa = 10.17 TDD71 pKa = 5.2 LIVTTALFLALSPGLLLTIPPGSGGLLRR99 pKa = 11.84 SGQTSLPAALTHH111 pKa = 5.98 AVVFAVVFALLRR123 pKa = 11.84 RR124 pKa = 11.84 QFPQFYY130 pKa = 10.62
Molecular weight: 13.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.911
IPC_protein 10.672
Toseland 10.818
ProMoST 10.511
Dawson 10.921
Bjellqvist 10.599
Wikipedia 11.111
Rodwell 11.228
Grimsley 10.965
Solomon 11.023
Lehninger 10.994
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.818
EMBOSS 11.213
Sillero 10.833
Patrickios 11.023
IPC_peptide 11.038
IPC2_peptide 9.37
IPC2.peptide.svr19 8.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
230
0
230
57286
65
3398
249.1
28.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.169 ± 0.291
1.637 ± 0.143
5.898 ± 0.172
6.761 ± 0.206
4.172 ± 0.101
6.256 ± 0.278
2.172 ± 0.137
5.998 ± 0.146
7.833 ± 0.41
7.978 ± 0.221
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.901 ± 0.117
5.85 ± 0.552
4.001 ± 0.22
3.5 ± 0.142
5.268 ± 0.185
5.682 ± 0.163
6.255 ± 0.198
6.639 ± 0.266
1.072 ± 0.079
3.956 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here