Moraxella phage Mcat6
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PH79|A0A0R6PH79_9CAUD Terminase OS=Moraxella phage Mcat6 OX=1647552 PE=4 SV=1
MM1 pKa = 7.2 SQFATLDD8 pKa = 3.58 EE9 pKa = 4.72 VKK11 pKa = 10.53 HH12 pKa = 5.6 HH13 pKa = 6.43 LRR15 pKa = 11.84 YY16 pKa = 10.34 DD17 pKa = 3.62 DD18 pKa = 3.87 TDD20 pKa = 3.73 SDD22 pKa = 4.56 EE23 pKa = 4.49 ILTIYY28 pKa = 9.62 LQSAQTAVKK37 pKa = 10.54 NYY39 pKa = 8.41 ITDD42 pKa = 4.59 EE43 pKa = 4.39 INDD46 pKa = 3.86 DD47 pKa = 3.87 MLPALKK53 pKa = 10.44 VATLLLVGYY62 pKa = 10.51 LDD64 pKa = 5.13 DD65 pKa = 5.2 NRR67 pKa = 11.84 NSEE70 pKa = 4.13 NGAEE74 pKa = 4.14 FGNYY78 pKa = 9.11 LPAPVRR84 pKa = 11.84 QMLAPYY90 pKa = 7.83 RR91 pKa = 11.84 TPTFF95 pKa = 3.48
Molecular weight: 10.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.255
IPC2_protein 4.317
IPC_protein 4.228
Toseland 4.037
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.164
Rodwell 4.062
Grimsley 3.948
Solomon 4.215
Lehninger 4.164
Nozaki 4.342
DTASelect 4.571
Thurlkill 4.088
EMBOSS 4.164
Sillero 4.355
Patrickios 3.35
IPC_peptide 4.215
IPC2_peptide 4.342
IPC2.peptide.svr19 4.268
Protein with the highest isoelectric point:
>tr|A0A0R6PHC8|A0A0R6PHC8_9CAUD Uncharacterized protein OS=Moraxella phage Mcat6 OX=1647552 PE=4 SV=1
MM1 pKa = 7.7 AKK3 pKa = 10.25 LGNLPPRR10 pKa = 11.84 LKK12 pKa = 9.39 PTQSHH17 pKa = 4.64 TPKK20 pKa = 10.51 RR21 pKa = 11.84 NWGKK25 pKa = 10.32 GRR27 pKa = 11.84 EE28 pKa = 4.11 GRR30 pKa = 11.84 AWRR33 pKa = 11.84 RR34 pKa = 11.84 LRR36 pKa = 11.84 AKK38 pKa = 9.92 MLVRR42 pKa = 11.84 DD43 pKa = 4.06 NYY45 pKa = 9.72 TCQLCGRR52 pKa = 11.84 IGGRR56 pKa = 11.84 LEE58 pKa = 4.59 LDD60 pKa = 3.78 HH61 pKa = 6.99 IVNKK65 pKa = 10.57 AVGGTDD71 pKa = 4.2 DD72 pKa = 4.03 KK73 pKa = 11.72 TNLQILCYY81 pKa = 9.44 QCHH84 pKa = 4.95 KK85 pKa = 9.73 TKK87 pKa = 9.37 TQSEE91 pKa = 4.72 SKK93 pKa = 10.7 AGGVLQFLGG102 pKa = 3.78
Molecular weight: 11.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 9.502
IPC_protein 9.663
Toseland 10.599
ProMoST 10.116
Dawson 10.672
Bjellqvist 10.306
Wikipedia 10.804
Rodwell 11.155
Grimsley 10.701
Solomon 10.73
Lehninger 10.716
Nozaki 10.599
DTASelect 10.277
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.599
Patrickios 10.906
IPC_peptide 10.745
IPC2_peptide 9.268
IPC2.peptide.svr19 8.512
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
12519
38
1174
223.6
24.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.659 ± 0.515
0.983 ± 0.196
6.382 ± 0.266
5.408 ± 0.204
3.507 ± 0.177
6.63 ± 0.355
2.388 ± 0.235
6.814 ± 0.227
6.973 ± 0.262
8.699 ± 0.248
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.436 ± 0.194
5.136 ± 0.233
3.459 ± 0.336
4.849 ± 0.202
4.321 ± 0.229
6.422 ± 0.491
6.502 ± 0.396
5.799 ± 0.285
1.222 ± 0.106
3.411 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here