Geobacter sp. DSM 2909 
Average proteome isoelectric point is 6.69 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 4159 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A2T5RE77|A0A2T5RE77_9DELT AlpA family transcriptional regulator OS=Geobacter sp. DSM 2909 OX=2135459 GN=C7376_101171 PE=4 SV=1MM1 pKa = 7.86  DD2 pKa = 4.07  FEE4 pKa = 5.16  EE5 pKa = 4.7  PQFHH9 pKa = 6.35  YY10 pKa = 10.58  WDD12 pKa = 3.93  VFPKK16 pKa = 8.94  TVKK19 pKa = 10.41  VSLTGWSVTIPLSVRR34 pKa = 11.84  GAPTGQIEE42 pKa = 4.79  FEE44 pKa = 4.35  SADD47 pKa = 3.89  SNIAWVDD54 pKa = 2.94  EE55 pKa = 4.16  DD56 pKa = 4.31  GRR58 pKa = 11.84  LNLGWQAGATVVMAYY73 pKa = 10.32  DD74 pKa = 3.47  SEE76 pKa = 4.47  NRR78 pKa = 11.84  DD79 pKa = 3.3  SVRR82 pKa = 11.84  YY83 pKa = 8.89  IQVEE87 pKa = 4.31  VVDD90 pKa = 3.91  YY91 pKa = 9.97  GQNGGGGYY99 pKa = 10.25  DD100 pKa = 3.45  GYY102 pKa = 11.49  GYY104 pKa = 10.28  DD105 pKa = 3.86  YY106 pKa = 8.18  PTT108 pKa = 3.79  
 12.05 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.782 
IPC2_protein 3.999 
IPC_protein 3.935 
Toseland    3.732 
ProMoST     4.088 
Dawson      3.923 
Bjellqvist  4.075 
Wikipedia   3.859 
Rodwell     3.757 
Grimsley    3.643 
Solomon     3.897 
Lehninger   3.859 
Nozaki      4.037 
DTASelect   4.253 
Thurlkill   3.783 
EMBOSS      3.859 
Sillero     4.05 
Patrickios  0.896 
IPC_peptide 3.897 
IPC2_peptide  4.024 
IPC2.peptide.svr19  3.933 
 Protein with the highest isoelectric point: 
>tr|A0A2T5R736|A0A2T5R736_9DELT L D-transpeptidase ErfK/SrfK OS=Geobacter sp. DSM 2909 OX=2135459 GN=C7376_11459 PE=3 SV=1MM1 pKa = 8.14  DD2 pKa = 4.81  SLRR5 pKa = 11.84  KK6 pKa = 9.49  LVAGVTRR13 pKa = 11.84  RR14 pKa = 11.84  YY15 pKa = 9.44  PIHH18 pKa = 6.49  TKK20 pKa = 10.67  SFMTWKK26 pKa = 10.51  SRR28 pKa = 11.84  FHH30 pKa = 7.58  RR31 pKa = 11.84  SNTDD35 pKa = 2.61  GNGFRR40 pKa = 11.84  RR41 pKa = 11.84  FLSAIGTICADD52 pKa = 3.72  KK53 pKa = 10.71  PLADD57 pKa = 3.75  PCRR60 pKa = 11.84  EE61 pKa = 3.64  LRR63 pKa = 11.84  IRR65 pKa = 11.84  AFLYY69 pKa = 10.71  VDD71 pKa = 4.69  FIWRR75 pKa = 11.84  GCC77 pKa = 3.51  
 9.02 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.351 
IPC2_protein 9.619 
IPC_protein 10.526 
Toseland    10.716 
ProMoST     10.467 
Dawson      10.804 
Bjellqvist  10.555 
Wikipedia   11.038 
Rodwell     10.935 
Grimsley    10.847 
Solomon     10.965 
Lehninger   10.935 
Nozaki      10.73 
DTASelect   10.54 
Thurlkill   10.716 
EMBOSS      11.125 
Sillero     10.745 
Patrickios  10.716 
IPC_peptide 10.965 
IPC2_peptide  9.867 
IPC2.peptide.svr19  8.54 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        4159 
0
4159 
1352630
29
3828
325.2
35.96
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        9.011 ± 0.041
1.32 ± 0.017
5.461 ± 0.038
6.799 ± 0.041
4.179 ± 0.026
8.13 ± 0.056
1.942 ± 0.017
5.959 ± 0.029
4.747 ± 0.039
10.086 ± 0.044
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.449 ± 0.016
3.127 ± 0.03
4.628 ± 0.028
2.724 ± 0.023
6.981 ± 0.043
6.053 ± 0.03
5.267 ± 0.029
7.299 ± 0.03
1.046 ± 0.014
2.789 ± 0.021
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here