Geobacter sp. DSM 2909
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4159 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T5RE77|A0A2T5RE77_9DELT AlpA family transcriptional regulator OS=Geobacter sp. DSM 2909 OX=2135459 GN=C7376_101171 PE=4 SV=1
MM1 pKa = 7.86 DD2 pKa = 4.07 FEE4 pKa = 5.16 EE5 pKa = 4.7 PQFHH9 pKa = 6.35 YY10 pKa = 10.58 WDD12 pKa = 3.93 VFPKK16 pKa = 8.94 TVKK19 pKa = 10.41 VSLTGWSVTIPLSVRR34 pKa = 11.84 GAPTGQIEE42 pKa = 4.79 FEE44 pKa = 4.35 SADD47 pKa = 3.89 SNIAWVDD54 pKa = 2.94 EE55 pKa = 4.16 DD56 pKa = 4.31 GRR58 pKa = 11.84 LNLGWQAGATVVMAYY73 pKa = 10.32 DD74 pKa = 3.47 SEE76 pKa = 4.47 NRR78 pKa = 11.84 DD79 pKa = 3.3 SVRR82 pKa = 11.84 YY83 pKa = 8.89 IQVEE87 pKa = 4.31 VVDD90 pKa = 3.91 YY91 pKa = 9.97 GQNGGGGYY99 pKa = 10.25 DD100 pKa = 3.45 GYY102 pKa = 11.49 GYY104 pKa = 10.28 DD105 pKa = 3.86 YY106 pKa = 8.18 PTT108 pKa = 3.79
Molecular weight: 12.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.999
IPC_protein 3.935
Toseland 3.732
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.859
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.05
Patrickios 0.896
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.933
Protein with the highest isoelectric point:
>tr|A0A2T5R736|A0A2T5R736_9DELT L D-transpeptidase ErfK/SrfK OS=Geobacter sp. DSM 2909 OX=2135459 GN=C7376_11459 PE=3 SV=1
MM1 pKa = 8.14 DD2 pKa = 4.81 SLRR5 pKa = 11.84 KK6 pKa = 9.49 LVAGVTRR13 pKa = 11.84 RR14 pKa = 11.84 YY15 pKa = 9.44 PIHH18 pKa = 6.49 TKK20 pKa = 10.67 SFMTWKK26 pKa = 10.51 SRR28 pKa = 11.84 FHH30 pKa = 7.58 RR31 pKa = 11.84 SNTDD35 pKa = 2.61 GNGFRR40 pKa = 11.84 RR41 pKa = 11.84 FLSAIGTICADD52 pKa = 3.72 KK53 pKa = 10.71 PLADD57 pKa = 3.75 PCRR60 pKa = 11.84 EE61 pKa = 3.64 LRR63 pKa = 11.84 IRR65 pKa = 11.84 AFLYY69 pKa = 10.71 VDD71 pKa = 4.69 FIWRR75 pKa = 11.84 GCC77 pKa = 3.51
Molecular weight: 9.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.619
IPC_protein 10.526
Toseland 10.716
ProMoST 10.467
Dawson 10.804
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.935
Grimsley 10.847
Solomon 10.965
Lehninger 10.935
Nozaki 10.73
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.965
IPC2_peptide 9.867
IPC2.peptide.svr19 8.54
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4159
0
4159
1352630
29
3828
325.2
35.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.011 ± 0.041
1.32 ± 0.017
5.461 ± 0.038
6.799 ± 0.041
4.179 ± 0.026
8.13 ± 0.056
1.942 ± 0.017
5.959 ± 0.029
4.747 ± 0.039
10.086 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.449 ± 0.016
3.127 ± 0.03
4.628 ± 0.028
2.724 ± 0.023
6.981 ± 0.043
6.053 ± 0.03
5.267 ± 0.029
7.299 ± 0.03
1.046 ± 0.014
2.789 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here