Klebsiella phage vB_KpnP_IME337

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; unclassified Drulisvirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6CUL9|A0A5J6CUL9_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_IME337 OX=2601650 PE=4 SV=1
MM1 pKa = 7.53SIKK4 pKa = 10.12PGSIVEE10 pKa = 4.02MLEE13 pKa = 4.44LGPEE17 pKa = 4.53PIDD20 pKa = 3.8PKK22 pKa = 10.59LALYY26 pKa = 7.34YY27 pKa = 10.97APGTQHH33 pKa = 6.81RR34 pKa = 11.84VIGYY38 pKa = 9.86DD39 pKa = 3.19PVTWEE44 pKa = 4.25VEE46 pKa = 4.64LINPDD51 pKa = 3.73NGSEE55 pKa = 4.05DD56 pKa = 3.63PGDD59 pKa = 3.42GVTFFPGEE67 pKa = 3.81YY68 pKa = 10.1KK69 pKa = 10.88LIVEE73 pKa = 4.84

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6CUF5|A0A5J6CUF5_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_IME337 OX=2601650 PE=4 SV=1
MM1 pKa = 7.7ASRR4 pKa = 11.84YY5 pKa = 9.16GKK7 pKa = 10.03NPEE10 pKa = 3.92TLLMRR15 pKa = 11.84RR16 pKa = 11.84QQPTIDD22 pKa = 3.41GLARR26 pKa = 11.84EE27 pKa = 4.55YY28 pKa = 10.64SAKK31 pKa = 10.43AALRR35 pKa = 11.84QHH37 pKa = 6.15YY38 pKa = 7.59EE39 pKa = 3.85KK40 pKa = 10.33QAKK43 pKa = 8.74RR44 pKa = 11.84LGMTLRR50 pKa = 11.84GYY52 pKa = 10.27CYY54 pKa = 9.65RR55 pKa = 11.84FNVRR59 pKa = 11.84GIVV62 pKa = 3.12

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13893

38

1243

267.2

29.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.199 ± 0.587

1.13 ± 0.192

6.183 ± 0.241

5.816 ± 0.4

3.095 ± 0.135

7.752 ± 0.386

1.843 ± 0.23

4.355 ± 0.191

5.003 ± 0.328

8.594 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.793 ± 0.249

4.11 ± 0.24

3.714 ± 0.244

4.729 ± 0.334

5.924 ± 0.293

6.442 ± 0.35

5.809 ± 0.348

6.996 ± 0.203

1.49 ± 0.116

4.024 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski