Natrialba phage PhiCh1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Myohalovirus; Natrialba virus PhiCh1

Average proteome isoelectric point is 4.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8JKZ3|Q8JKZ3_9CAUD Uncharacterized protein OS=Natrialba phage PhiCh1 OX=114777 GN=PhiCh1_290 PE=4 SV=1
MM1 pKa = 7.91VSEE4 pKa = 4.4SDD6 pKa = 3.52EE7 pKa = 4.26FACANCEE14 pKa = 4.03EE15 pKa = 4.34VHH17 pKa = 7.23DD18 pKa = 4.75DD19 pKa = 3.75RR20 pKa = 11.84GDD22 pKa = 4.4AIACCGDD29 pKa = 2.72IPMVVSRR36 pKa = 11.84GPAGTTVKK44 pKa = 10.75GPDD47 pKa = 3.18PAAFADD53 pKa = 4.13ATDD56 pKa = 3.56SHH58 pKa = 7.19PGGEE62 pKa = 4.39ADD64 pKa = 4.55VEE66 pKa = 4.34

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8JL46|Q8JL46_9CAUD Capsid protein gpB OS=Natrialba phage PhiCh1 OX=114777 PE=4 SV=1
MM1 pKa = 7.12PTVARR6 pKa = 11.84PRR8 pKa = 11.84SPTATRR14 pKa = 11.84AATAKK19 pKa = 10.02ARR21 pKa = 11.84RR22 pKa = 11.84ASRR25 pKa = 11.84PTGRR29 pKa = 11.84LRR31 pKa = 11.84PSGCSRR37 pKa = 11.84SRR39 pKa = 11.84TASRR43 pKa = 11.84RR44 pKa = 11.84TPRR47 pKa = 11.84ISRR50 pKa = 11.84RR51 pKa = 11.84WRR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.52RR56 pKa = 11.84ATASSWTTTAKK67 pKa = 10.26LAPRR71 pKa = 11.84RR72 pKa = 11.84TLTKK76 pKa = 10.32SRR78 pKa = 11.84TTAKK82 pKa = 9.81SRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84SSGCRR91 pKa = 11.84STDD94 pKa = 3.23NQHH97 pKa = 4.27THH99 pKa = 5.36

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

18337

54

581

187.1

20.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.578 ± 0.351

0.987 ± 0.127

10.105 ± 0.344

10.214 ± 0.351

2.841 ± 0.12

7.242 ± 0.238

2.35 ± 0.18

4.619 ± 0.207

2.596 ± 0.166

6.98 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.164

2.874 ± 0.14

4.614 ± 0.208

2.967 ± 0.158

6.811 ± 0.353

6.839 ± 0.374

6.13 ± 0.271

6.98 ± 0.268

1.674 ± 0.108

2.476 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski