Treponema phagedenis F0421

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema phagedenis

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3146 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7NWI8|E7NWI8_TREPH PBS lyase HEAT-like repeat protein OS=Treponema phagedenis F0421 OX=754027 GN=HMPREF9554_02453 PE=4 SV=1
II1 pKa = 6.74TKK3 pKa = 9.94VSEE6 pKa = 4.46TISFDD11 pKa = 4.01DD12 pKa = 4.47DD13 pKa = 3.77CTFGNVEE20 pKa = 4.41TKK22 pKa = 10.68LSNGWTVTQKK32 pKa = 10.45FSWSFDD38 pKa = 3.08SYY40 pKa = 11.28YY41 pKa = 10.65EE42 pKa = 4.1PEE44 pKa = 4.04IDD46 pKa = 4.03YY47 pKa = 10.94QCEE50 pKa = 4.05DD51 pKa = 3.77VGDD54 pKa = 3.97LSIFDD59 pKa = 4.89KK60 pKa = 11.45NMEE63 pKa = 4.48PYY65 pKa = 10.78SNEE68 pKa = 3.74LTSEE72 pKa = 4.14EE73 pKa = 3.89EE74 pKa = 3.92KK75 pKa = 10.75AIQRR79 pKa = 11.84LCIKK83 pKa = 10.58DD84 pKa = 3.71ADD86 pKa = 4.12EE87 pKa = 4.55LTDD90 pKa = 4.53AVYY93 pKa = 10.36QQTDD97 pKa = 3.12WKK99 pKa = 10.76SLAEE103 pKa = 4.06EE104 pKa = 3.73VRR106 pKa = 11.84EE107 pKa = 4.09YY108 pKa = 11.55NKK110 pKa = 10.45NPYY113 pKa = 9.5SYY115 pKa = 11.38YY116 pKa = 10.93GVTPLDD122 pKa = 4.42FII124 pKa = 5.75

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7NXD5|E7NXD5_TREPH Uncharacterized protein (Fragment) OS=Treponema phagedenis F0421 OX=754027 GN=HMPREF9554_02754 PE=4 SV=1
RR1 pKa = 7.29QNQTVVSNSLYY12 pKa = 9.24QFRR15 pKa = 11.84VRR17 pKa = 11.84YY18 pKa = 8.92IKK20 pKa = 10.77NLFEE24 pKa = 4.14IVKK27 pKa = 9.09EE28 pKa = 3.86RR29 pKa = 11.84LVGIISVFGLKK40 pKa = 10.54LSRR43 pKa = 11.84GSCCTAVRR51 pKa = 11.84PNN53 pKa = 3.19

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3146

0

3146

824179

37

2111

262.0

29.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.888 ± 0.052

1.233 ± 0.019

4.906 ± 0.034

6.762 ± 0.056

5.247 ± 0.044

6.195 ± 0.041

1.657 ± 0.019

8.167 ± 0.048

7.767 ± 0.043

9.616 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.022

4.625 ± 0.038

3.656 ± 0.029

3.441 ± 0.026

4.165 ± 0.034

6.357 ± 0.032

5.554 ± 0.033

5.941 ± 0.039

0.872 ± 0.016

3.539 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski