Bacteroides intestinalis CAG:564

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2827 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5MC35|R5MC35_9BACE 3-dehydroquinate synthase OS=Bacteroides intestinalis CAG:564 OX=1263049 GN=BN711_01090 PE=4 SV=1
MM1 pKa = 7.15MCMTAVTFSACSDD14 pKa = 4.09DD15 pKa = 5.93DD16 pKa = 6.48DD17 pKa = 5.6DD18 pKa = 5.7DD19 pKa = 6.09KK20 pKa = 11.8SINVPEE26 pKa = 4.46SVVQALKK33 pKa = 10.48QKK35 pKa = 9.71YY36 pKa = 8.66PSATGIEE43 pKa = 4.34WEE45 pKa = 4.17QKK47 pKa = 8.26GTYY50 pKa = 9.34FVADD54 pKa = 3.49CWLDD58 pKa = 3.57GKK60 pKa = 10.61EE61 pKa = 4.15SNIWFDD67 pKa = 4.71PNANWLMTEE76 pKa = 5.69SEE78 pKa = 4.57IFWNDD83 pKa = 2.77LPEE86 pKa = 4.42AVQTAFGSGEE96 pKa = 4.0YY97 pKa = 10.86ANWVQDD103 pKa = 3.45DD104 pKa = 5.49YY105 pKa = 12.15YY106 pKa = 11.47FLLYY110 pKa = 9.72PLQPMQYY117 pKa = 9.8VIEE120 pKa = 4.35VKK122 pKa = 10.43QGNQKK127 pKa = 9.48YY128 pKa = 8.17QLFYY132 pKa = 11.4SEE134 pKa = 5.93DD135 pKa = 3.57GGLMNTVNVTGKK147 pKa = 10.26DD148 pKa = 3.39DD149 pKa = 4.1TIYY152 pKa = 10.32PPKK155 pKa = 10.52VV156 pKa = 3.02

Molecular weight: 17.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5MEF9|R5MEF9_9BACE Cyclic nucleotide-binding domain protein OS=Bacteroides intestinalis CAG:564 OX=1263049 GN=BN711_00187 PE=4 SV=1
MM1 pKa = 7.48LNVDD5 pKa = 4.09IGWFCTNFIAFEE17 pKa = 4.17VGVILSISSRR27 pKa = 11.84RR28 pKa = 11.84FTLNNKK34 pKa = 9.26IPFLYY39 pKa = 10.55FLDD42 pKa = 4.1EE43 pKa = 4.16ATLCFSRR50 pKa = 11.84KK51 pKa = 6.75QRR53 pKa = 11.84SRR55 pKa = 11.84LRR57 pKa = 11.84RR58 pKa = 11.84FTGNMAAAAA67 pKa = 4.14

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2827

0

2827

1100216

29

2484

389.2

44.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.947 ± 0.047

1.249 ± 0.016

5.553 ± 0.03

6.453 ± 0.041

4.557 ± 0.025

6.864 ± 0.037

1.877 ± 0.021

6.769 ± 0.041

6.46 ± 0.034

9.062 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.02

5.248 ± 0.032

3.821 ± 0.027

3.523 ± 0.024

4.558 ± 0.029

6.169 ± 0.034

5.73 ± 0.031

6.28 ± 0.036

1.458 ± 0.02

4.709 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski