Escherichia phage EcS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Winklervirus; unclassified Winklervirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5ZBZ6|A0A2Z5ZBZ6_9CAUD Anaerobic ribonucleoside-triphosphate reductase-activating protein OS=Escherichia phage EcS1 OX=2083276 PE=3 SV=1
MM1 pKa = 7.33TKK3 pKa = 10.09QEE5 pKa = 4.44IMDD8 pKa = 3.98LQLAGLFVKK17 pKa = 10.4EE18 pKa = 4.36LDD20 pKa = 3.69DD21 pKa = 5.54DD22 pKa = 4.1RR23 pKa = 11.84VLVEE27 pKa = 4.23GTSPAGLDD35 pKa = 3.47YY36 pKa = 11.03LIEE39 pKa = 4.27EE40 pKa = 4.72DD41 pKa = 6.02FEE43 pKa = 4.95AFWVYY48 pKa = 10.55EE49 pKa = 3.8ATTTGNYY56 pKa = 9.69ISVDD60 pKa = 3.17AFGGFDD66 pKa = 4.89DD67 pKa = 6.71AYY69 pKa = 10.92DD70 pKa = 3.79CAKK73 pKa = 10.61NLQQ76 pKa = 3.53

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5ZCM8|A0A2Z5ZCM8_9CAUD Baseplate wedge tail fiber connector OS=Escherichia phage EcS1 OX=2083276 PE=4 SV=1
MM1 pKa = 7.18MLITTDD7 pKa = 4.33SIMKK11 pKa = 8.72TWLSYY16 pKa = 10.47GRR18 pKa = 11.84AHH20 pKa = 7.17PRR22 pKa = 11.84TGRR25 pKa = 11.84RR26 pKa = 11.84WYY28 pKa = 10.8LEE30 pKa = 3.84AVCRR34 pKa = 11.84EE35 pKa = 3.58TGFRR39 pKa = 11.84VNSKK43 pKa = 9.07FACKK47 pKa = 8.29PTKK50 pKa = 10.02NQVRR54 pKa = 11.84KK55 pKa = 7.96FKK57 pKa = 10.4RR58 pKa = 11.84WARR61 pKa = 11.84HH62 pKa = 4.23QIEE65 pKa = 4.83FKK67 pKa = 10.93LYY69 pKa = 8.4WDD71 pKa = 4.9EE72 pKa = 4.05II73 pKa = 4.29

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

295

0

295

54697

38

1258

185.4

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.94 ± 0.171

1.132 ± 0.062

6.446 ± 0.109

7.017 ± 0.175

4.371 ± 0.107

6.163 ± 0.176

1.916 ± 0.081

7.017 ± 0.11

7.419 ± 0.19

7.627 ± 0.145

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.938 ± 0.091

5.145 ± 0.126

3.488 ± 0.095

3.437 ± 0.107

4.391 ± 0.119

6.249 ± 0.118

6.081 ± 0.225

6.633 ± 0.104

1.506 ± 0.061

4.082 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski