Macaca mulatta papillomavirus 3
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385AHR9|A0A385AHR9_9PAPI Regulatory protein E2 OS=Macaca mulatta papillomavirus 3 OX=2294151 GN=E2 PE=3 SV=1
MM1 pKa = 7.45 HH2 pKa = 7.27 GAKK5 pKa = 8.12 PTLSDD10 pKa = 2.97 IVLALEE16 pKa = 4.55 PPPEE20 pKa = 4.43 AVSLHH25 pKa = 6.54 CNEE28 pKa = 4.44 QLDD31 pKa = 4.0 SSEE34 pKa = 5.14 EE35 pKa = 3.92 EE36 pKa = 4.73 DD37 pKa = 4.74 GDD39 pKa = 5.08 DD40 pKa = 3.69 GLDD43 pKa = 3.62 YY44 pKa = 10.69 IVEE47 pKa = 4.24 GDD49 pKa = 4.17 EE50 pKa = 4.7 EE51 pKa = 5.46 DD52 pKa = 5.52 DD53 pKa = 4.78 EE54 pKa = 4.49 PTEE57 pKa = 4.09 PAGQAIYY64 pKa = 10.36 RR65 pKa = 11.84 VVTSCGEE72 pKa = 3.78 CGRR75 pKa = 11.84 AVRR78 pKa = 11.84 LAVRR82 pKa = 11.84 SSEE85 pKa = 3.69 ADD87 pKa = 2.88 IRR89 pKa = 11.84 ALQDD93 pKa = 3.68 LLLGSLSIVCPVCAA107 pKa = 4.8
Molecular weight: 11.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.897
IPC_protein 3.846
Toseland 3.656
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.567
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.101
Thurlkill 3.694
EMBOSS 3.732
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.863
Protein with the highest isoelectric point:
>tr|A0A385AHF8|A0A385AHF8_9PAPI Protein E7 OS=Macaca mulatta papillomavirus 3 OX=2294151 GN=E7 PE=3 SV=1
MM1 pKa = 7.34 TLNSTLYY8 pKa = 10.1 LAPSKK13 pKa = 11.22 YY14 pKa = 9.95 PLLQLLSSQTPTTPTRR30 pKa = 11.84 PIPAAAPPGAPRR42 pKa = 11.84 KK43 pKa = 9.78 DD44 pKa = 2.96 KK45 pKa = 11.02 SRR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 6.15 LYY51 pKa = 11.02 LNDD54 pKa = 4.55 PDD56 pKa = 5.06 PDD58 pKa = 4.14 SDD60 pKa = 4.61 CSCSSPTLRR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 LEE74 pKa = 4.19 RR75 pKa = 11.84 WDD77 pKa = 3.28 GWHH80 pKa = 6.7 PQQAPWTVSAKK91 pKa = 10.66 GNTVTLTATGVNGPTVSVTLHH112 pKa = 6.06 LL113 pKa = 4.6
Molecular weight: 12.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.242
IPC2_protein 9.297
IPC_protein 9.575
Toseland 9.94
ProMoST 9.706
Dawson 10.175
Bjellqvist 9.911
Wikipedia 10.365
Rodwell 10.409
Grimsley 10.248
Solomon 10.248
Lehninger 10.204
Nozaki 10.028
DTASelect 9.882
Thurlkill 10.014
EMBOSS 10.35
Sillero 10.116
Patrickios 10.087
IPC_peptide 10.233
IPC2_peptide 8.946
IPC2.peptide.svr19 8.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2372
107
649
338.9
37.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.01 ± 0.271
2.53 ± 0.418
6.282 ± 0.507
4.933 ± 0.665
3.373 ± 0.459
7.04 ± 0.455
2.403 ± 0.325
3.668 ± 0.338
4.553 ± 0.704
8.558 ± 0.508
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.813 ± 0.392
2.867 ± 0.441
6.83 ± 0.925
4.637 ± 0.479
5.818 ± 0.231
8.179 ± 0.523
6.492 ± 0.913
6.872 ± 0.39
1.602 ± 0.317
3.541 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here