bank vole virus 1
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4PJ44|A0A2H4PJ44_9MONO Attachment protein OS=bank vole virus 1 OX=2756244 GN=G PE=3 SV=1
MM1 pKa = 7.39 SGVLSALKK9 pKa = 10.02 EE10 pKa = 4.12 FKK12 pKa = 10.03 DD13 pKa = 3.25 AKK15 pKa = 10.34 LRR17 pKa = 11.84 PKK19 pKa = 10.5 HH20 pKa = 6.87 DD21 pKa = 3.29 GLTRR25 pKa = 11.84 GAITSLKK32 pKa = 10.04 HH33 pKa = 4.95 KK34 pKa = 9.96 VAVIVPGQEE43 pKa = 3.7 GSKK46 pKa = 10.54 VRR48 pKa = 11.84 WQLLKK53 pKa = 11.14 LLIGVAWSDD62 pKa = 3.2 AANPSVQTGVMLSLISLISEE82 pKa = 4.21 SPANMVRR89 pKa = 11.84 RR90 pKa = 11.84 LNNDD94 pKa = 3.08 PDD96 pKa = 3.76 LAITIVEE103 pKa = 3.91 FTINSEE109 pKa = 4.08 GEE111 pKa = 3.73 LRR113 pKa = 11.84 FASRR117 pKa = 11.84 GMSYY121 pKa = 10.91 DD122 pKa = 3.73 DD123 pKa = 4.4 QMSTYY128 pKa = 11.06 LRR130 pKa = 11.84 MRR132 pKa = 11.84 DD133 pKa = 3.71 NAPQAAQGEE142 pKa = 4.84 HH143 pKa = 6.67 PFEE146 pKa = 5.54 EE147 pKa = 4.22 SDD149 pKa = 4.03 AWNQDD154 pKa = 3.88 DD155 pKa = 5.66 LPMDD159 pKa = 4.33 EE160 pKa = 5.18 YY161 pKa = 10.8 MIANTTVQVQLWNLLIKK178 pKa = 10.59 AVTAPDD184 pKa = 3.51 TARR187 pKa = 11.84 DD188 pKa = 3.61 SEE190 pKa = 4.05 QRR192 pKa = 11.84 RR193 pKa = 11.84 WLKK196 pKa = 9.9 FVQQRR201 pKa = 11.84 RR202 pKa = 11.84 VEE204 pKa = 4.14 AFYY207 pKa = 11.04 KK208 pKa = 10.45 LHH210 pKa = 6.82 SIWADD215 pKa = 3.25 KK216 pKa = 10.42 VRR218 pKa = 11.84 NLLAADD224 pKa = 4.28 LSVRR228 pKa = 11.84 RR229 pKa = 11.84 YY230 pKa = 8.67 MIRR233 pKa = 11.84 TLIEE237 pKa = 3.86 IQRR240 pKa = 11.84 MGQTKK245 pKa = 9.91 GRR247 pKa = 11.84 LLEE250 pKa = 4.35 VIADD254 pKa = 3.39 IGNYY258 pKa = 9.27 IEE260 pKa = 4.31 EE261 pKa = 4.44 SGLAGFQLTIRR272 pKa = 11.84 YY273 pKa = 9.32 GIEE276 pKa = 3.46 TRR278 pKa = 11.84 FAALALNEE286 pKa = 4.18 FQGDD290 pKa = 3.56 LATIEE295 pKa = 4.7 RR296 pKa = 11.84 LMKK299 pKa = 10.39 LYY301 pKa = 11.16 LEE303 pKa = 4.86 MGPTAPFMVLLEE315 pKa = 5.44 DD316 pKa = 3.55 STQTKK321 pKa = 8.98 FAPGNYY327 pKa = 8.46 PLLWSYY333 pKa = 12.11 AMGVGSALDD342 pKa = 3.12 RR343 pKa = 11.84 AMSNLNFNRR352 pKa = 11.84 PYY354 pKa = 9.94 MDD356 pKa = 2.96 YY357 pKa = 11.0 GYY359 pKa = 10.84 FRR361 pKa = 11.84 LGYY364 pKa = 9.84 KK365 pKa = 9.74 IVRR368 pKa = 11.84 QSEE371 pKa = 4.17 GSIDD375 pKa = 3.27 SRR377 pKa = 11.84 MANEE381 pKa = 5.13 LEE383 pKa = 5.66 LSPQDD388 pKa = 3.71 QARR391 pKa = 11.84 LRR393 pKa = 11.84 ALVSGIGGRR402 pKa = 11.84 EE403 pKa = 3.66 DD404 pKa = 3.44 VEE406 pKa = 4.49 NIEE409 pKa = 4.12 ARR411 pKa = 11.84 GGNFQIADD419 pKa = 3.37 IEE421 pKa = 4.51 TTEE424 pKa = 4.08 GEE426 pKa = 4.27 EE427 pKa = 4.2 LAGDD431 pKa = 4.03 EE432 pKa = 4.22 AQEE435 pKa = 4.05 LPRR438 pKa = 11.84 TRR440 pKa = 11.84 RR441 pKa = 11.84 TRR443 pKa = 11.84 RR444 pKa = 11.84 ARR446 pKa = 11.84 SARR449 pKa = 11.84 SSRR452 pKa = 11.84 AQAGAGDD459 pKa = 5.02 GININSYY466 pKa = 10.21 SRR468 pKa = 11.84 AVEE471 pKa = 3.72 EE472 pKa = 4.24 ALRR475 pKa = 11.84 GSLTDD480 pKa = 5.25 RR481 pKa = 11.84 EE482 pKa = 4.46 DD483 pKa = 3.3 QSGDD487 pKa = 3.34 QLYY490 pKa = 10.93 NSPDD494 pKa = 3.3 YY495 pKa = 9.79 TRR497 pKa = 11.84 EE498 pKa = 3.95 MEE500 pKa = 4.23 TEE502 pKa = 4.13 EE503 pKa = 4.17 EE504 pKa = 5.06 DD505 pKa = 4.07 EE506 pKa = 6.89 DD507 pKa = 4.07 EE508 pKa = 5.08 GQDD511 pKa = 3.38 NPLGLRR517 pKa = 11.84 YY518 pKa = 9.7 NDD520 pKa = 3.52 EE521 pKa = 4.09 SLLRR525 pKa = 4.7
Molecular weight: 59.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.986
IPC2_protein 4.965
IPC_protein 4.902
Toseland 4.774
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.698
Rodwell 4.749
Grimsley 4.685
Solomon 4.851
Lehninger 4.8
Nozaki 4.952
DTASelect 5.105
Thurlkill 4.762
EMBOSS 4.724
Sillero 5.029
Patrickios 4.304
IPC_peptide 4.851
IPC2_peptide 5.016
IPC2.peptide.svr19 5.034
Protein with the highest isoelectric point:
>tr|A0A2H4PJ60|A0A2H4PJ60_9MONO Isoform of A0A2H4PJ62 C protein OS=bank vole virus 1 OX=2756244 GN=P\V\C PE=4 SV=1
MM1 pKa = 7.8 PSKK4 pKa = 10.31 LWKK7 pKa = 9.79 SLKK10 pKa = 9.81 KK11 pKa = 10.58 LRR13 pKa = 11.84 VPRR16 pKa = 11.84 PRR18 pKa = 11.84 NNSVPEE24 pKa = 4.3 STSPTPTSHH33 pKa = 7.58 DD34 pKa = 3.21 QTTDD38 pKa = 3.03 PEE40 pKa = 4.17 VRR42 pKa = 11.84 VRR44 pKa = 11.84 IGVRR48 pKa = 11.84 RR49 pKa = 11.84 NPDD52 pKa = 2.97 LVGVEE57 pKa = 4.13 RR58 pKa = 11.84 QTKK61 pKa = 8.25 VLKK64 pKa = 10.26 QDD66 pKa = 3.5 LALVILTQLRR76 pKa = 11.84 DD77 pKa = 3.6 LEE79 pKa = 4.53 KK80 pKa = 10.4 DD81 pKa = 3.6 YY82 pKa = 10.59 PAQVPHH88 pKa = 7.45 IITSLKK94 pKa = 10.18 YY95 pKa = 9.44 STLQFVKK102 pKa = 9.48 TILMRR107 pKa = 11.84 VMEE110 pKa = 4.64 GLPINSTWVRR120 pKa = 11.84 QVEE123 pKa = 4.5 EE124 pKa = 4.68 YY125 pKa = 10.36 ICTTSQEE132 pKa = 4.2 VQNLKK137 pKa = 10.54 EE138 pKa = 3.84 AVEE141 pKa = 4.42 WVRR144 pKa = 11.84 KK145 pKa = 8.5 MMEE148 pKa = 4.28 DD149 pKa = 3.51 QDD151 pKa = 3.88 RR152 pKa = 11.84 TT153 pKa = 3.67
Molecular weight: 17.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.844
IPC2_protein 8.756
IPC_protein 8.756
Toseland 9.648
ProMoST 9.268
Dawson 9.823
Bjellqvist 9.472
Wikipedia 9.955
Rodwell 10.233
Grimsley 9.867
Solomon 9.897
Lehninger 9.867
Nozaki 9.677
DTASelect 9.458
Thurlkill 9.692
EMBOSS 10.043
Sillero 9.75
Patrickios 9.823
IPC_peptide 9.897
IPC2_peptide 7.98
IPC2.peptide.svr19 7.711
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
1
7
4962
153
2206
708.9
79.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.784 ± 0.68
1.653 ± 0.276
5.361 ± 0.446
5.482 ± 0.537
3.083 ± 0.51
5.744 ± 0.582
2.559 ± 0.517
7.517 ± 0.691
5.038 ± 0.573
10.238 ± 0.559
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.257 ± 0.314
5.28 ± 0.442
4.938 ± 0.461
3.99 ± 0.423
5.401 ± 0.59
8.283 ± 0.716
6.308 ± 0.67
5.865 ± 0.772
1.028 ± 0.247
4.152 ± 0.653
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here