Lactococcus phage asccphi28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; unclassified Salasmaviridae

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1ABJ6|B1ABJ6_9CAUD Uncharacterized protein OS=Lactococcus phage asccphi28 OX=503388 PE=4 SV=1
MM1 pKa = 8.42AEE3 pKa = 4.74EE4 pKa = 4.21LTQAQLIEE12 pKa = 4.37QKK14 pKa = 10.82LQLTQMEE21 pKa = 4.95LEE23 pKa = 4.22ASTSFEE29 pKa = 4.99AISEE33 pKa = 4.11MLLGIMDD40 pKa = 4.61SEE42 pKa = 5.18AILYY46 pKa = 7.38TVQIQAIHH54 pKa = 6.82KK55 pKa = 9.48LQDD58 pKa = 2.93IYY60 pKa = 11.59YY61 pKa = 8.09GTASRR66 pKa = 11.84LEE68 pKa = 4.17EE69 pKa = 3.85AVHH72 pKa = 7.06KK73 pKa = 10.99NEE75 pKa = 4.2QLKK78 pKa = 11.1ANNLVLAGVKK88 pKa = 10.49NSDD91 pKa = 3.23IEE93 pKa = 4.44TEE95 pKa = 3.94LTEE98 pKa = 4.31EE99 pKa = 4.11EE100 pKa = 4.34KK101 pKa = 10.8EE102 pKa = 4.13YY103 pKa = 10.69LAVMEE108 pKa = 4.95DD109 pKa = 3.94SEE111 pKa = 5.44GEE113 pKa = 3.97

Molecular weight: 12.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1ABJ1|B1ABJ1_9CAUD Upper collar OS=Lactococcus phage asccphi28 OX=503388 PE=4 SV=1
MM1 pKa = 7.11TLLMVVRR8 pKa = 11.84EE9 pKa = 4.18TLMKK13 pKa = 10.49QLTGFNSTIRR23 pKa = 11.84HH24 pKa = 5.2LMEE27 pKa = 5.07KK28 pKa = 9.29FTSKK32 pKa = 10.62PIIAHH37 pKa = 6.02KK38 pKa = 9.95RR39 pKa = 11.84LSVRR43 pKa = 11.84GGYY46 pKa = 9.66LVV48 pKa = 3.36

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

1

28

5840

48

722

208.6

23.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.36 ± 0.445

0.702 ± 0.131

5.616 ± 0.316

6.507 ± 0.784

4.623 ± 0.437

5.325 ± 0.578

1.764 ± 0.165

7.055 ± 0.276

8.185 ± 0.78

8.339 ± 0.653

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.242

7.5 ± 0.47

3.031 ± 0.328

4.623 ± 0.409

3.116 ± 0.313

6.147 ± 0.411

7.363 ± 0.513

5.805 ± 0.336

1.37 ± 0.239

4.863 ± 0.47
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski