Influenza B virus (strain B/Lee/1940)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Betainfluenzavirus; Influenza B virus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9QLL6|PB2_INBLE Polymerase basic protein 2 OS=Influenza B virus (strain B/Lee/1940) OX=518987 GN=PB2 PE=1 SV=1
MM1 pKa = 7.72DD2 pKa = 4.07TFITKK7 pKa = 9.94NFQTTIIQKK16 pKa = 10.29AKK18 pKa = 8.68NTMAEE23 pKa = 4.08FSEE26 pKa = 4.98DD27 pKa = 3.91PEE29 pKa = 4.24LQPAVLFNICVHH41 pKa = 6.7LEE43 pKa = 3.78VCYY46 pKa = 10.78VISDD50 pKa = 3.68MNFLDD55 pKa = 4.78EE56 pKa = 4.48EE57 pKa = 4.92GKK59 pKa = 9.08TYY61 pKa = 9.54TALEE65 pKa = 4.39GQGKK69 pKa = 7.68EE70 pKa = 3.94QNLRR74 pKa = 11.84PQYY77 pKa = 10.54EE78 pKa = 4.57VIEE81 pKa = 4.19GMPRR85 pKa = 11.84NIAWMVQRR93 pKa = 11.84SLAQEE98 pKa = 4.15HH99 pKa = 6.56GIEE102 pKa = 4.27TPRR105 pKa = 11.84YY106 pKa = 8.63LADD109 pKa = 3.7LFDD112 pKa = 4.32YY113 pKa = 8.3KK114 pKa = 9.99TKK116 pKa = 10.82RR117 pKa = 11.84FIEE120 pKa = 4.0VGITKK125 pKa = 10.32GLADD129 pKa = 4.42DD130 pKa = 4.63YY131 pKa = 11.19FWKK134 pKa = 10.59KK135 pKa = 10.28KK136 pKa = 9.87EE137 pKa = 3.82KK138 pKa = 10.49LGNSMEE144 pKa = 4.41LMIFSYY150 pKa = 10.74NQDD153 pKa = 2.94YY154 pKa = 11.15SLSDD158 pKa = 3.39EE159 pKa = 4.4SSLDD163 pKa = 3.54EE164 pKa = 4.39EE165 pKa = 4.91GKK167 pKa = 10.97GRR169 pKa = 11.84VLSRR173 pKa = 11.84LTEE176 pKa = 4.09LQAEE180 pKa = 4.6LSLKK184 pKa = 10.43NLWQVLIGEE193 pKa = 4.37EE194 pKa = 4.42EE195 pKa = 3.98IEE197 pKa = 4.22KK198 pKa = 11.23GIDD201 pKa = 3.36FKK203 pKa = 11.4LGQTISKK210 pKa = 9.34LRR212 pKa = 11.84NISVPAGFSNFEE224 pKa = 4.0GMRR227 pKa = 11.84SYY229 pKa = 11.04IDD231 pKa = 4.25NIDD234 pKa = 3.38PKK236 pKa = 11.14GAIEE240 pKa = 4.13RR241 pKa = 11.84NLARR245 pKa = 11.84MSPLVSVTPKK255 pKa = 9.85KK256 pKa = 10.83LKK258 pKa = 10.23WEE260 pKa = 4.3DD261 pKa = 3.41LRR263 pKa = 11.84PIGPHH268 pKa = 6.43IYY270 pKa = 9.79NHH272 pKa = 6.28EE273 pKa = 4.17LPEE276 pKa = 4.04VPYY279 pKa = 10.96NAFLLMSDD287 pKa = 3.99EE288 pKa = 4.95LGLANMTEE296 pKa = 4.57GKK298 pKa = 9.69SKK300 pKa = 10.66KK301 pKa = 9.72PKK303 pKa = 9.48TLAKK307 pKa = 9.86EE308 pKa = 3.88CLEE311 pKa = 4.37RR312 pKa = 11.84YY313 pKa = 7.57STLRR317 pKa = 11.84DD318 pKa = 3.24QTDD321 pKa = 4.33PILIMKK327 pKa = 9.41SEE329 pKa = 4.03KK330 pKa = 10.78ANEE333 pKa = 3.86NFLWRR338 pKa = 11.84LWRR341 pKa = 11.84DD342 pKa = 3.5CVNTISNEE350 pKa = 3.92EE351 pKa = 4.17TGNEE355 pKa = 3.95LQKK358 pKa = 10.18TNYY361 pKa = 9.82AKK363 pKa = 10.3WATGDD368 pKa = 3.24GLTYY372 pKa = 10.2QKK374 pKa = 10.63IMKK377 pKa = 9.33EE378 pKa = 3.96VAIDD382 pKa = 3.82DD383 pKa = 3.53EE384 pKa = 4.71TMYY387 pKa = 10.76QEE389 pKa = 4.61EE390 pKa = 4.16PKK392 pKa = 10.33IPNKK396 pKa = 10.07CRR398 pKa = 11.84VAAWVQAEE406 pKa = 4.25MNLLSTLTSKK416 pKa = 10.85RR417 pKa = 11.84ALDD420 pKa = 3.91LPEE423 pKa = 5.27IGPDD427 pKa = 3.43VAPVEE432 pKa = 4.56HH433 pKa = 6.53VGSEE437 pKa = 3.58RR438 pKa = 11.84RR439 pKa = 11.84KK440 pKa = 9.81YY441 pKa = 9.86FVNEE445 pKa = 3.34INYY448 pKa = 9.25CKK450 pKa = 10.56ASTVMMKK457 pKa = 10.46YY458 pKa = 10.74VLFHH462 pKa = 6.42TSLLNEE468 pKa = 4.68SNASMGKK475 pKa = 9.99YY476 pKa = 9.62KK477 pKa = 10.59VIPITNRR484 pKa = 11.84VVNEE488 pKa = 3.96KK489 pKa = 11.04GEE491 pKa = 4.32SFDD494 pKa = 3.5MLYY497 pKa = 11.24GLAVKK502 pKa = 9.91GQSHH506 pKa = 6.71LRR508 pKa = 11.84GDD510 pKa = 3.87TDD512 pKa = 3.58VVTVVTFEE520 pKa = 4.42FSSTDD525 pKa = 3.3PRR527 pKa = 11.84VDD529 pKa = 3.05SGKK532 pKa = 8.47WPKK535 pKa = 9.4YY536 pKa = 7.19TVFKK540 pKa = 10.52IGSLFVSGRR549 pKa = 11.84EE550 pKa = 3.9KK551 pKa = 10.45PVYY554 pKa = 10.01LYY556 pKa = 11.09CRR558 pKa = 11.84VNGTNKK564 pKa = 9.61IQMKK568 pKa = 8.84WGMEE572 pKa = 3.68ARR574 pKa = 11.84RR575 pKa = 11.84CLLQSMQQMEE585 pKa = 5.01AIVDD589 pKa = 3.85QEE591 pKa = 4.33SSIQGYY597 pKa = 11.18DD598 pKa = 3.05MTKK601 pKa = 10.52ACFKK605 pKa = 10.54GDD607 pKa = 3.06RR608 pKa = 11.84VNNPKK613 pKa = 9.16TFSIGTQEE621 pKa = 3.93GKK623 pKa = 10.13LVKK626 pKa = 10.6GSFGKK631 pKa = 10.38ALRR634 pKa = 11.84VIFTKK639 pKa = 10.82CLMHH643 pKa = 6.24YY644 pKa = 10.55VFGNAQLEE652 pKa = 4.61GFSAEE657 pKa = 4.01SRR659 pKa = 11.84RR660 pKa = 11.84LLLLIQALKK669 pKa = 10.37DD670 pKa = 3.68RR671 pKa = 11.84KK672 pKa = 10.3GPWVFDD678 pKa = 3.76LEE680 pKa = 4.23GMYY683 pKa = 10.42FGVEE687 pKa = 3.99EE688 pKa = 5.11CISNNPWVIQSAYY701 pKa = 8.73WFNEE705 pKa = 3.17WLGIEE710 pKa = 4.2KK711 pKa = 10.34EE712 pKa = 4.23GSKK715 pKa = 10.48VLEE718 pKa = 4.8SIDD721 pKa = 4.39EE722 pKa = 4.35IMDD725 pKa = 3.59EE726 pKa = 4.19

Molecular weight: 83.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P06817|VNB_INBLE Glycoprotein NB OS=Influenza B virus (strain B/Lee/1940) OX=518987 GN=NB PE=1 SV=1
MM1 pKa = 7.86SNMDD5 pKa = 3.51IDD7 pKa = 4.77SINTGTIDD15 pKa = 3.52KK16 pKa = 9.66TPEE19 pKa = 3.76EE20 pKa = 4.5LTPGTSGATRR30 pKa = 11.84PIIKK34 pKa = 9.3PATLAPPSNKK44 pKa = 8.5RR45 pKa = 11.84TRR47 pKa = 11.84NPSPEE52 pKa = 3.93RR53 pKa = 11.84TTTSSEE59 pKa = 3.53TDD61 pKa = 2.75IGRR64 pKa = 11.84KK65 pKa = 7.2IQKK68 pKa = 9.44KK69 pKa = 5.81QTPTEE74 pKa = 4.07IKK76 pKa = 9.51KK77 pKa = 10.27SVYY80 pKa = 10.67KK81 pKa = 10.08MVVKK85 pKa = 10.78LGEE88 pKa = 4.6FYY90 pKa = 11.28NQMMVKK96 pKa = 10.47AGLNDD101 pKa = 3.47DD102 pKa = 4.0MEE104 pKa = 5.91RR105 pKa = 11.84NLIQNAQAVEE115 pKa = 4.5RR116 pKa = 11.84ILLAATDD123 pKa = 4.01DD124 pKa = 4.89KK125 pKa = 10.92KK126 pKa = 9.49TEE128 pKa = 4.0YY129 pKa = 9.71QKK131 pKa = 11.0KK132 pKa = 9.79RR133 pKa = 11.84NARR136 pKa = 11.84DD137 pKa = 3.37VKK139 pKa = 10.45EE140 pKa = 4.19GKK142 pKa = 10.27EE143 pKa = 4.03EE144 pKa = 3.84IDD146 pKa = 3.46HH147 pKa = 6.46NKK149 pKa = 9.42TGGTFYY155 pKa = 11.63KK156 pKa = 9.77MVRR159 pKa = 11.84DD160 pKa = 3.85DD161 pKa = 3.34KK162 pKa = 10.95TIYY165 pKa = 10.34FSPIKK170 pKa = 9.7ITFLKK175 pKa = 10.81EE176 pKa = 3.78EE177 pKa = 4.34VKK179 pKa = 10.15TMYY182 pKa = 8.64KK183 pKa = 7.92TTMGSDD189 pKa = 3.29GFSGLNHH196 pKa = 6.33IMIGHH201 pKa = 5.5SQMNDD206 pKa = 2.51VCFQRR211 pKa = 11.84SKK213 pKa = 9.93GLKK216 pKa = 9.68RR217 pKa = 11.84VGLDD221 pKa = 3.12PSLISTFAGSTLPRR235 pKa = 11.84RR236 pKa = 11.84SGTTGVAIKK245 pKa = 10.82GGGTLVDD252 pKa = 3.34EE253 pKa = 5.92AIRR256 pKa = 11.84FIGRR260 pKa = 11.84AMADD264 pKa = 3.07RR265 pKa = 11.84GLLRR269 pKa = 11.84DD270 pKa = 4.04IKK272 pKa = 11.18AKK274 pKa = 9.04TAYY277 pKa = 9.86EE278 pKa = 4.34KK279 pKa = 10.71ILLNLKK285 pKa = 9.2NKK287 pKa = 10.06CSAPQQKK294 pKa = 10.63ALVDD298 pKa = 3.53QVIGSRR304 pKa = 11.84NPGIADD310 pKa = 3.55IEE312 pKa = 4.43DD313 pKa = 3.74LTLLARR319 pKa = 11.84SMVVVRR325 pKa = 11.84PSVASKK331 pKa = 10.56VVLPISIYY339 pKa = 11.18AKK341 pKa = 10.05IPQLGFNTEE350 pKa = 4.14EE351 pKa = 4.38YY352 pKa = 11.58SMVGYY357 pKa = 10.14EE358 pKa = 5.9AMALYY363 pKa = 11.21NMATPVSILRR373 pKa = 11.84MGDD376 pKa = 3.35DD377 pKa = 4.8AKK379 pKa = 11.28DD380 pKa = 3.32KK381 pKa = 11.01SQLFFMSCFGAAYY394 pKa = 9.59EE395 pKa = 4.14DD396 pKa = 4.13LRR398 pKa = 11.84VLSALTGTEE407 pKa = 4.23FKK409 pKa = 10.48PRR411 pKa = 11.84SALKK415 pKa = 10.56CKK417 pKa = 10.08GFHH420 pKa = 6.04VPAKK424 pKa = 9.45EE425 pKa = 3.85QVEE428 pKa = 4.49GMGAALMSIKK438 pKa = 10.49LQFWAPMTRR447 pKa = 11.84SGGNEE452 pKa = 3.52VSGEE456 pKa = 4.13GGSGQISCSPVFAVEE471 pKa = 4.22RR472 pKa = 11.84PIALSKK478 pKa = 9.66QAVRR482 pKa = 11.84RR483 pKa = 11.84MLSMNVEE490 pKa = 3.96GRR492 pKa = 11.84DD493 pKa = 3.42ADD495 pKa = 3.91VKK497 pKa = 11.31GNLLKK502 pKa = 10.64MMNDD506 pKa = 2.88SMAKK510 pKa = 8.11KK511 pKa = 8.72TSGNAFIGKK520 pKa = 9.8KK521 pKa = 8.63MFQISDD527 pKa = 3.7KK528 pKa = 11.21NKK530 pKa = 9.17VNPIEE535 pKa = 4.46IPIKK539 pKa = 8.61QTIPNFFFGRR549 pKa = 11.84DD550 pKa = 3.22TAEE553 pKa = 5.21DD554 pKa = 4.25YY555 pKa = 11.47DD556 pKa = 4.45DD557 pKa = 5.76LDD559 pKa = 3.91YY560 pKa = 11.81

Molecular weight: 61.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

4718

100

770

428.9

48.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.104 ± 0.42

2.056 ± 0.305

5.002 ± 0.312

6.973 ± 0.699

3.603 ± 0.267

7.27 ± 0.389

1.696 ± 0.302

6.783 ± 0.439

7.694 ± 0.313

8.923 ± 0.462

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.858 ± 0.344

5.341 ± 0.487

4.133 ± 0.34

3.243 ± 0.352

5.129 ± 0.412

6.507 ± 0.403

6.231 ± 0.479

5.384 ± 0.275

1.166 ± 0.221

2.904 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski