Amino acid dipepetide frequency for Escherichia phage PBECO4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.369AlaAla: 3.369 ± 0.247
0.571AlaCys: 0.571 ± 0.081
3.152AlaAsp: 3.152 ± 0.18
3.586AlaGlu: 3.586 ± 0.204
2.029AlaPhe: 2.029 ± 0.154
2.926AlaGly: 2.926 ± 0.228
0.64AlaHis: 0.64 ± 0.074
3.458AlaIle: 3.458 ± 0.178
3.625AlaLys: 3.625 ± 0.223
4.246AlaLeu: 4.246 ± 0.219
1.369AlaMet: 1.369 ± 0.129
2.788AlaAsn: 2.788 ± 0.189
1.773AlaPro: 1.773 ± 0.216
2.01AlaGln: 2.01 ± 0.173
1.911AlaArg: 1.911 ± 0.114
2.896AlaSer: 2.896 ± 0.221
2.561AlaThr: 2.561 ± 0.251
3.507AlaVal: 3.507 ± 0.246
0.581AlaTrp: 0.581 ± 0.07
2.148AlaTyr: 2.148 ± 0.146
0.0AlaXaa: 0.0 ± 0.0
Cys
0.542CysAla: 0.542 ± 0.083
0.167CysCys: 0.167 ± 0.047
0.887CysAsp: 0.887 ± 0.096
0.828CysGlu: 0.828 ± 0.096
0.552CysPhe: 0.552 ± 0.068
0.847CysGly: 0.847 ± 0.131
0.236CysHis: 0.236 ± 0.043
1.3CysIle: 1.3 ± 0.127
0.857CysLys: 0.857 ± 0.085
0.808CysLeu: 0.808 ± 0.094
0.227CysMet: 0.227 ± 0.048
0.828CysAsn: 0.828 ± 0.089
0.424CysPro: 0.424 ± 0.066
0.384CysGln: 0.384 ± 0.064
0.414CysArg: 0.414 ± 0.062
0.965CysSer: 0.965 ± 0.094
1.241CysThr: 1.241 ± 0.134
0.946CysVal: 0.946 ± 0.099
0.158CysTrp: 0.158 ± 0.035
0.532CysTyr: 0.532 ± 0.073
0.0CysXaa: 0.0 ± 0.0
Asp
3.655AspAla: 3.655 ± 0.193
0.798AspCys: 0.798 ± 0.107
4.325AspAsp: 4.325 ± 0.232
4.906AspGlu: 4.906 ± 0.275
3.271AspPhe: 3.271 ± 0.187
4.768AspGly: 4.768 ± 0.223
0.729AspHis: 0.729 ± 0.101
5.95AspIle: 5.95 ± 0.292
4.138AspLys: 4.138 ± 0.208
5.073AspLeu: 5.073 ± 0.262
2.118AspMet: 2.118 ± 0.147
3.832AspAsn: 3.832 ± 0.212
2.088AspPro: 2.088 ± 0.157
1.192AspGln: 1.192 ± 0.106
2.0AspArg: 2.0 ± 0.137
4.738AspSer: 4.738 ± 0.226
3.714AspThr: 3.714 ± 0.215
4.729AspVal: 4.729 ± 0.27
0.739AspTrp: 0.739 ± 0.082
3.684AspTyr: 3.684 ± 0.203
0.0AspXaa: 0.0 ± 0.0
Glu
3.192GluAla: 3.192 ± 0.198
0.956GluCys: 0.956 ± 0.107
4.256GluAsp: 4.256 ± 0.275
5.261GluGlu: 5.261 ± 0.327
3.438GluPhe: 3.438 ± 0.187
2.926GluGly: 2.926 ± 0.198
1.537GluHis: 1.537 ± 0.127
5.438GluIle: 5.438 ± 0.229
4.098GluLys: 4.098 ± 0.214
6.531GluLeu: 6.531 ± 0.298
2.581GluMet: 2.581 ± 0.168
3.714GluAsn: 3.714 ± 0.195
2.039GluPro: 2.039 ± 0.139
2.699GluGln: 2.699 ± 0.179
2.492GluArg: 2.492 ± 0.151
4.236GluSer: 4.236 ± 0.208
3.369GluThr: 3.369 ± 0.193
4.65GluVal: 4.65 ± 0.269
0.956GluTrp: 0.956 ± 0.105
4.207GluTyr: 4.207 ± 0.241
0.0GluXaa: 0.0 ± 0.0
Phe
1.724PheAla: 1.724 ± 0.116
0.857PheCys: 0.857 ± 0.097
3.428PheAsp: 3.428 ± 0.212
2.768PheGlu: 2.768 ± 0.143
1.852PhePhe: 1.852 ± 0.157
2.936PheGly: 2.936 ± 0.181
0.778PheHis: 0.778 ± 0.091
3.891PheIle: 3.891 ± 0.194
3.665PheLys: 3.665 ± 0.204
2.611PheLeu: 2.611 ± 0.163
1.419PheMet: 1.419 ± 0.115
3.29PheAsn: 3.29 ± 0.177
1.222PhePro: 1.222 ± 0.104
1.852PheGln: 1.852 ± 0.127
1.586PheArg: 1.586 ± 0.12
3.182PheSer: 3.182 ± 0.178
2.551PheThr: 2.551 ± 0.152
2.955PheVal: 2.955 ± 0.189
0.581PheTrp: 0.581 ± 0.08
2.207PheTyr: 2.207 ± 0.148
0.0PheXaa: 0.0 ± 0.0
Gly
2.758GlyAla: 2.758 ± 0.239
0.828GlyCys: 0.828 ± 0.101
3.093GlyAsp: 3.093 ± 0.233
3.724GlyGlu: 3.724 ± 0.224
2.611GlyPhe: 2.611 ± 0.137
3.044GlyGly: 3.044 ± 0.263
0.837GlyHis: 0.837 ± 0.078
3.95GlyIle: 3.95 ± 0.212
4.029GlyLys: 4.029 ± 0.233
3.921GlyLeu: 3.921 ± 0.202
1.497GlyMet: 1.497 ± 0.127
3.96GlyAsn: 3.96 ± 0.221
0.709GlyPro: 0.709 ± 0.09
1.704GlyGln: 1.704 ± 0.156
2.098GlyArg: 2.098 ± 0.158
4.315GlySer: 4.315 ± 0.268
4.926GlyThr: 4.926 ± 0.307
4.0GlyVal: 4.0 ± 0.26
0.601GlyTrp: 0.601 ± 0.088
2.995GlyTyr: 2.995 ± 0.193
0.0GlyXaa: 0.0 ± 0.0
His
0.788HisAla: 0.788 ± 0.092
0.276HisCys: 0.276 ± 0.055
1.497HisAsp: 1.497 ± 0.138
1.34HisGlu: 1.34 ± 0.128
0.877HisPhe: 0.877 ± 0.096
0.995HisGly: 0.995 ± 0.1
0.394HisHis: 0.394 ± 0.063
1.359HisIle: 1.359 ± 0.115
1.448HisLys: 1.448 ± 0.129
1.291HisLeu: 1.291 ± 0.133
0.611HisMet: 0.611 ± 0.084
1.291HisAsn: 1.291 ± 0.118
0.709HisPro: 0.709 ± 0.087
0.483HisGln: 0.483 ± 0.069
0.749HisArg: 0.749 ± 0.085
1.182HisSer: 1.182 ± 0.117
0.995HisThr: 0.995 ± 0.091
0.965HisVal: 0.965 ± 0.098
0.256HisTrp: 0.256 ± 0.052
0.946HisTyr: 0.946 ± 0.105
0.0HisXaa: 0.0 ± 0.0
Ile
3.546IleAla: 3.546 ± 0.187
0.857IleCys: 0.857 ± 0.103
5.97IleAsp: 5.97 ± 0.247
5.438IleGlu: 5.438 ± 0.258
2.561IlePhe: 2.561 ± 0.156
3.881IleGly: 3.881 ± 0.198
1.891IleHis: 1.891 ± 0.15
5.29IleIle: 5.29 ± 0.27
5.753IleLys: 5.753 ± 0.245
5.004IleLeu: 5.004 ± 0.239
1.911IleMet: 1.911 ± 0.146
5.034IleAsn: 5.034 ± 0.256
2.699IlePro: 2.699 ± 0.16
3.103IleGln: 3.103 ± 0.187
3.33IleArg: 3.33 ± 0.189
5.832IleSer: 5.832 ± 0.334
4.699IleThr: 4.699 ± 0.252
4.325IleVal: 4.325 ± 0.201
0.749IleTrp: 0.749 ± 0.096
2.926IleTyr: 2.926 ± 0.153
0.0IleXaa: 0.0 ± 0.0
Lys
3.34LysAla: 3.34 ± 0.205
0.946LysCys: 0.946 ± 0.106
4.492LysAsp: 4.492 ± 0.2
5.724LysGlu: 5.724 ± 0.323
3.448LysPhe: 3.448 ± 0.197
2.788LysGly: 2.788 ± 0.161
1.379LysHis: 1.379 ± 0.126
5.034LysIle: 5.034 ± 0.256
5.27LysLys: 5.27 ± 0.285
5.408LysLeu: 5.408 ± 0.252
2.63LysMet: 2.63 ± 0.189
4.315LysAsn: 4.315 ± 0.181
2.0LysPro: 2.0 ± 0.15
2.325LysGln: 2.325 ± 0.186
2.857LysArg: 2.857 ± 0.194
4.522LysSer: 4.522 ± 0.244
4.039LysThr: 4.039 ± 0.194
4.64LysVal: 4.64 ± 0.189
0.591LysTrp: 0.591 ± 0.08
4.275LysTyr: 4.275 ± 0.219
0.0LysXaa: 0.0 ± 0.0
Leu
3.842LeuAla: 3.842 ± 0.216
1.182LeuCys: 1.182 ± 0.101
5.477LeuAsp: 5.477 ± 0.249
4.975LeuGlu: 4.975 ± 0.222
3.005LeuPhe: 3.005 ± 0.177
4.138LeuGly: 4.138 ± 0.25
1.527LeuHis: 1.527 ± 0.12
4.975LeuIle: 4.975 ± 0.207
5.733LeuLys: 5.733 ± 0.262
5.536LeuLeu: 5.536 ± 0.259
2.62LeuMet: 2.62 ± 0.154
5.202LeuAsn: 5.202 ± 0.256
3.044LeuPro: 3.044 ± 0.182
3.064LeuGln: 3.064 ± 0.188
3.152LeuArg: 3.152 ± 0.173
5.96LeuSer: 5.96 ± 0.269
4.335LeuThr: 4.335 ± 0.208
5.044LeuVal: 5.044 ± 0.221
0.877LeuTrp: 0.877 ± 0.089
4.187LeuTyr: 4.187 ± 0.245
0.0LeuXaa: 0.0 ± 0.0
Met
1.557MetAla: 1.557 ± 0.119
0.364MetCys: 0.364 ± 0.054
1.675MetAsp: 1.675 ± 0.144
1.625MetGlu: 1.625 ± 0.142
1.428MetPhe: 1.428 ± 0.12
1.182MetGly: 1.182 ± 0.115
0.601MetHis: 0.601 ± 0.075
2.049MetIle: 2.049 ± 0.151
2.64MetLys: 2.64 ± 0.18
2.226MetLeu: 2.226 ± 0.159
0.857MetMet: 0.857 ± 0.085
1.98MetAsn: 1.98 ± 0.135
0.69MetPro: 0.69 ± 0.081
1.064MetGln: 1.064 ± 0.095
1.084MetArg: 1.084 ± 0.106
2.118MetSer: 2.118 ± 0.146
1.822MetThr: 1.822 ± 0.116
1.557MetVal: 1.557 ± 0.11
0.335MetTrp: 0.335 ± 0.051
1.872MetTyr: 1.872 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
3.241AsnAla: 3.241 ± 0.186
0.67AsnCys: 0.67 ± 0.097
3.872AsnAsp: 3.872 ± 0.195
4.138AsnGlu: 4.138 ± 0.226
2.611AsnPhe: 2.611 ± 0.15
4.748AsnGly: 4.748 ± 0.272
1.113AsnHis: 1.113 ± 0.103
5.487AsnIle: 5.487 ± 0.239
3.891AsnLys: 3.891 ± 0.19
4.354AsnLeu: 4.354 ± 0.196
1.517AsnMet: 1.517 ± 0.135
4.394AsnAsn: 4.394 ± 0.237
2.601AsnPro: 2.601 ± 0.164
1.882AsnGln: 1.882 ± 0.149
2.217AsnArg: 2.217 ± 0.139
4.354AsnSer: 4.354 ± 0.238
4.64AsnThr: 4.64 ± 0.23
4.187AsnVal: 4.187 ± 0.205
0.483AsnTrp: 0.483 ± 0.073
2.768AsnTyr: 2.768 ± 0.157
0.0AsnXaa: 0.0 ± 0.0
Pro
1.625ProAla: 1.625 ± 0.159
0.296ProCys: 0.296 ± 0.054
2.591ProAsp: 2.591 ± 0.148
2.946ProGlu: 2.946 ± 0.17
1.379ProPhe: 1.379 ± 0.094
1.586ProGly: 1.586 ± 0.132
0.404ProHis: 0.404 ± 0.059
2.049ProIle: 2.049 ± 0.139
2.039ProLys: 2.039 ± 0.15
2.118ProLeu: 2.118 ± 0.143
0.64ProMet: 0.64 ± 0.074
2.01ProAsn: 2.01 ± 0.145
0.916ProPro: 0.916 ± 0.115
0.975ProGln: 0.975 ± 0.096
0.985ProArg: 0.985 ± 0.087
2.611ProSer: 2.611 ± 0.171
1.901ProThr: 1.901 ± 0.171
2.808ProVal: 2.808 ± 0.204
0.355ProTrp: 0.355 ± 0.055
1.694ProTyr: 1.694 ± 0.133
0.0ProXaa: 0.0 ± 0.0
Gln
1.803GlnAla: 1.803 ± 0.189
0.562GlnCys: 0.562 ± 0.081
2.01GlnAsp: 2.01 ± 0.152
2.601GlnGlu: 2.601 ± 0.181
1.724GlnPhe: 1.724 ± 0.129
1.97GlnGly: 1.97 ± 0.161
0.798GlnHis: 0.798 ± 0.073
2.187GlnIle: 2.187 ± 0.134
2.217GlnLys: 2.217 ± 0.142
3.133GlnLeu: 3.133 ± 0.188
1.103GlnMet: 1.103 ± 0.108
1.951GlnAsn: 1.951 ± 0.167
1.015GlnPro: 1.015 ± 0.117
1.685GlnGln: 1.685 ± 0.138
1.379GlnArg: 1.379 ± 0.115
2.443GlnSer: 2.443 ± 0.178
1.724GlnThr: 1.724 ± 0.118
1.951GlnVal: 1.951 ± 0.145
0.364GlnTrp: 0.364 ± 0.063
2.689GlnTyr: 2.689 ± 0.187
0.0GlnXaa: 0.0 ± 0.0
Arg
1.842ArgAla: 1.842 ± 0.141
0.522ArgCys: 0.522 ± 0.082
2.197ArgAsp: 2.197 ± 0.182
2.325ArgGlu: 2.325 ± 0.137
1.832ArgPhe: 1.832 ± 0.139
2.02ArgGly: 2.02 ± 0.132
0.621ArgHis: 0.621 ± 0.084
2.867ArgIle: 2.867 ± 0.167
2.65ArgLys: 2.65 ± 0.153
2.837ArgLeu: 2.837 ± 0.16
1.162ArgMet: 1.162 ± 0.114
2.414ArgAsn: 2.414 ± 0.163
0.965ArgPro: 0.965 ± 0.1
1.625ArgGln: 1.625 ± 0.137
1.31ArgArg: 1.31 ± 0.118
2.512ArgSer: 2.512 ± 0.14
2.384ArgThr: 2.384 ± 0.169
2.157ArgVal: 2.157 ± 0.163
0.522ArgTrp: 0.522 ± 0.071
1.96ArgTyr: 1.96 ± 0.142
0.0ArgXaa: 0.0 ± 0.0
Ser
3.438SerAla: 3.438 ± 0.203
0.857SerCys: 0.857 ± 0.108
4.098SerAsp: 4.098 ± 0.221
4.098SerGlu: 4.098 ± 0.197
3.448SerPhe: 3.448 ± 0.161
4.541SerGly: 4.541 ± 0.289
0.995SerHis: 0.995 ± 0.105
5.477SerIle: 5.477 ± 0.239
4.926SerLys: 4.926 ± 0.217
5.802SerLeu: 5.802 ± 0.245
1.694SerMet: 1.694 ± 0.137
4.187SerAsn: 4.187 ± 0.204
2.049SerPro: 2.049 ± 0.121
1.891SerGln: 1.891 ± 0.158
2.079SerArg: 2.079 ± 0.145
5.586SerSer: 5.586 ± 0.358
6.64SerThr: 6.64 ± 0.264
5.31SerVal: 5.31 ± 0.23
0.63SerTrp: 0.63 ± 0.076
3.33SerTyr: 3.33 ± 0.186
0.0SerXaa: 0.0 ± 0.0
Thr
3.231ThrAla: 3.231 ± 0.192
0.493ThrCys: 0.493 ± 0.08
4.364ThrAsp: 4.364 ± 0.227
4.059ThrGlu: 4.059 ± 0.193
3.093ThrPhe: 3.093 ± 0.184
3.881ThrGly: 3.881 ± 0.282
1.093ThrHis: 1.093 ± 0.089
4.945ThrIle: 4.945 ± 0.216
3.97ThrLys: 3.97 ± 0.21
5.517ThrLeu: 5.517 ± 0.215
1.458ThrMet: 1.458 ± 0.114
3.96ThrAsn: 3.96 ± 0.218
2.522ThrPro: 2.522 ± 0.163
1.941ThrGln: 1.941 ± 0.166
2.059ThrArg: 2.059 ± 0.133
4.551ThrSer: 4.551 ± 0.307
4.029ThrThr: 4.029 ± 0.261
4.453ThrVal: 4.453 ± 0.229
0.709ThrTrp: 0.709 ± 0.089
3.31ThrTyr: 3.31 ± 0.182
0.0ThrXaa: 0.0 ± 0.0
Val
2.827ValAla: 2.827 ± 0.256
0.847ValCys: 0.847 ± 0.086
4.098ValAsp: 4.098 ± 0.234
4.246ValGlu: 4.246 ± 0.206
2.965ValPhe: 2.965 ± 0.171
2.847ValGly: 2.847 ± 0.181
1.645ValHis: 1.645 ± 0.141
4.64ValIle: 4.64 ± 0.212
4.67ValLys: 4.67 ± 0.204
7.152ValLeu: 7.152 ± 0.264
1.557ValMet: 1.557 ± 0.117
3.32ValAsn: 3.32 ± 0.189
3.024ValPro: 3.024 ± 0.232
3.221ValGln: 3.221 ± 0.194
2.443ValArg: 2.443 ± 0.156
4.857ValSer: 4.857 ± 0.216
3.773ValThr: 3.773 ± 0.273
4.177ValVal: 4.177 ± 0.219
0.709ValTrp: 0.709 ± 0.071
3.418ValTyr: 3.418 ± 0.182
0.0ValXaa: 0.0 ± 0.0
Trp
0.562TrpAla: 0.562 ± 0.075
0.236TrpCys: 0.236 ± 0.043
0.877TrpAsp: 0.877 ± 0.105
0.709TrpGlu: 0.709 ± 0.071
0.591TrpPhe: 0.591 ± 0.081
0.522TrpGly: 0.522 ± 0.074
0.207TrpHis: 0.207 ± 0.051
0.719TrpIle: 0.719 ± 0.093
0.68TrpLys: 0.68 ± 0.077
0.69TrpLeu: 0.69 ± 0.078
0.384TrpMet: 0.384 ± 0.06
0.719TrpAsn: 0.719 ± 0.095
0.128TrpPro: 0.128 ± 0.038
0.276TrpGln: 0.276 ± 0.053
0.394TrpArg: 0.394 ± 0.063
0.601TrpSer: 0.601 ± 0.072
0.699TrpThr: 0.699 ± 0.086
0.867TrpVal: 0.867 ± 0.081
0.167TrpTrp: 0.167 ± 0.045
0.808TrpTyr: 0.808 ± 0.088
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.266TyrAla: 2.266 ± 0.136
0.857TyrCys: 0.857 ± 0.095
4.088TyrAsp: 4.088 ± 0.26
3.28TyrGlu: 3.28 ± 0.189
2.64TyrPhe: 2.64 ± 0.157
3.054TyrGly: 3.054 ± 0.157
1.025TyrHis: 1.025 ± 0.11
3.734TyrIle: 3.734 ± 0.207
3.724TyrLys: 3.724 ± 0.208
3.615TyrLeu: 3.615 ± 0.181
1.359TyrMet: 1.359 ± 0.121
3.99TyrAsn: 3.99 ± 0.226
1.31TyrPro: 1.31 ± 0.112
1.931TyrGln: 1.931 ± 0.158
2.187TyrArg: 2.187 ± 0.145
3.615TyrSer: 3.615 ± 0.201
3.596TyrThr: 3.596 ± 0.211
3.212TyrVal: 3.212 ± 0.199
0.473TyrTrp: 0.473 ± 0.069
2.778TyrTyr: 2.778 ± 0.163
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 551 proteins (101510 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski