Desulfuromonas soudanensis
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3469 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M3QFF4|A0A0M3QFF4_9DELT Autotransporter assembly factor TamA OS=Desulfuromonas soudanensis OX=1603606 GN=DSOUD_1192 PE=3 SV=1
MM1 pKa = 7.56 SNLTPSPVIDD11 pKa = 4.72 AGTDD15 pKa = 3.25 QYY17 pKa = 12.07 ALTGKK22 pKa = 9.5 VVNLAGTSYY31 pKa = 11.32 DD32 pKa = 3.6 SAGGPLTYY40 pKa = 10.13 QWNLVSTPSGSATTLSGANTLTPSFTADD68 pKa = 3.25 LPGDD72 pKa = 3.83 YY73 pKa = 10.49 VLSLLADD80 pKa = 4.36 DD81 pKa = 6.42 GEE83 pKa = 4.65 LQSQTDD89 pKa = 4.06 TVTVHH94 pKa = 6.88 ANVINCTFDD103 pKa = 3.38 NPATLGMSGVIEE115 pKa = 4.18 TSLSGDD121 pKa = 3.35 IVPLCDD127 pKa = 3.43 GWVLSANTTDD137 pKa = 5.03 NRR139 pKa = 11.84 VEE141 pKa = 3.98 LQNVFTEE148 pKa = 4.12 EE149 pKa = 4.19 FEE151 pKa = 4.7 RR152 pKa = 11.84 SVSVDD157 pKa = 2.94 GAVRR161 pKa = 11.84 DD162 pKa = 3.84 MEE164 pKa = 4.91 FDD166 pKa = 3.99 DD167 pKa = 5.75 EE168 pKa = 5.21 SGLLYY173 pKa = 10.42 AVKK176 pKa = 10.28 KK177 pKa = 9.93 DD178 pKa = 3.57 LPSISRR184 pKa = 11.84 VDD186 pKa = 3.76 MQAGTANLIPLPSLPLDD203 pKa = 3.8 LALGGSNKK211 pKa = 9.91 LFVSLRR217 pKa = 11.84 SDD219 pKa = 3.5 PLNIYY224 pKa = 10.55 ADD226 pKa = 3.75 YY227 pKa = 11.44 SSISLVNGATGSVLKK242 pKa = 9.05 TWPDD246 pKa = 3.18 TSFSADD252 pKa = 3.97 SILLAYY258 pKa = 9.92 DD259 pKa = 3.63 RR260 pKa = 11.84 SGQQLVAGEE269 pKa = 4.2 VWINPAEE276 pKa = 4.25 LKK278 pKa = 10.41 RR279 pKa = 11.84 YY280 pKa = 10.09 AFDD283 pKa = 4.06 EE284 pKa = 4.78 GALTLNLVDD293 pKa = 3.17 QRR295 pKa = 11.84 GYY297 pKa = 11.12 VGTSAGDD304 pKa = 3.58 LQISPDD310 pKa = 3.28 GRR312 pKa = 11.84 HH313 pKa = 5.71 AAFTTGSGNGSVNPYY328 pKa = 9.79 TIFDD332 pKa = 4.66 FSTDD336 pKa = 3.31 DD337 pKa = 3.28 FGTVYY342 pKa = 11.09 GEE344 pKa = 4.23 WDD346 pKa = 3.14 TDD348 pKa = 3.59 AYY350 pKa = 9.42 PLSGSFSPDD359 pKa = 2.17 GNYY362 pKa = 9.37 FVATNGSAIQLFDD375 pKa = 3.65 VANHH379 pKa = 6.26 NLLAEE384 pKa = 4.38 YY385 pKa = 9.55 PVSCNGLLEE394 pKa = 4.43 TEE396 pKa = 4.18 MVTRR400 pKa = 11.84 FSRR403 pKa = 11.84 GGNILFAYY411 pKa = 6.82 GTCTGASSSTGRR423 pKa = 11.84 IFWQVIEE430 pKa = 4.18 PP431 pKa = 3.79
Molecular weight: 45.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.846
IPC_protein 3.872
Toseland 3.643
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.694
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.986
Patrickios 1.316
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|A0A0M3QER3|A0A0M3QER3_9DELT Chromosomal replication initiator protein DnaA OS=Desulfuromonas soudanensis OX=1603606 GN=dnaA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSKK9 pKa = 9.13 VKK11 pKa = 10.4 RR12 pKa = 11.84 KK13 pKa = 7.77 RR14 pKa = 11.84 THH16 pKa = 5.86 GFRR19 pKa = 11.84 KK20 pKa = 10.09 RR21 pKa = 11.84 MQTKK25 pKa = 9.99 NGQSVLKK32 pKa = 10.26 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.05 GRR39 pKa = 11.84 KK40 pKa = 8.49 ILAATIPVKK49 pKa = 10.79
Molecular weight: 5.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.427
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.149
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.981
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3469
0
3469
1153148
40
4379
332.4
36.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.067 ± 0.059
1.163 ± 0.023
5.223 ± 0.037
6.761 ± 0.054
4.074 ± 0.031
8.659 ± 0.048
1.964 ± 0.023
5.426 ± 0.039
3.958 ± 0.047
11.457 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.138 ± 0.019
2.862 ± 0.045
4.9 ± 0.03
3.084 ± 0.025
6.815 ± 0.059
5.471 ± 0.033
5.156 ± 0.061
7.205 ± 0.036
1.042 ± 0.018
2.578 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here